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Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

  • G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.

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Metadaten
Author:Noorsharmimi Omar, Qiuting Loh, Gee Jun Tye, Yee Siew Choong, Rahmah Noordin, Jörn GlöklerORCiD, Theam Soon Lim
URN:urn:nbn:de:kobv:526-opus4-6267
DOI:https://doi.org/10.3390/s140100346
ISSN:1424-8220
Parent Title (English):Sensors
Document Type:Article
Language:English
Year of Publication:2013
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/07/05
Tag:DNAzyme; G-quadruplex; antibody; gold nanoparticles
Volume:14
Issue:1
First Page:346
Last Page:355
Source:Omar, N.; Loh, Q.; Tye, G.J.; Choong, Y.S.; Noordin, R.; Glökler, J.; Lim, T.S. Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors 2014, 14, 346-355.
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY 3.0 - Namensnennung 3.0 Unported
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