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Electrophoretic µPAD for Purification and Analysis of DNA Samples

  • In this work, the fabrication and characterization of a simple, inexpensive, and effective microfluidic paper analytic device (µPAD) for monitoring DNA samples is reported. The glass microfiber-based chip has been fabricated by a new wax-based transfer-printing technique and an electrode printing process. It is capable of moving DNA effectively in a time-dependent fashion. The nucleic acid sample is not damaged by this process and is accumulated in front of the anode, but not directly on the electrode. Thus, further DNA processing is feasible. The system allows the DNA to be purified by separating it from other components in sample mixtures such as proteins. Furthermore, it is demonstrated that DNA can be moved through several layers of the glass fiber material. This proof of concept will provide the basis for the development of rapid test systems, e.g., for the detection of pathogens in water samples.

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Metadaten
Author:Natascha Katharina Heinsohn, Robert Raimund Niedl, Alexander Anielski, Fred LisdatORCiDGND, Carsten Beta
URN:urn:nbn:de:kobv:526-opus4-15763
DOI (Version of Record):https://doi.org/10.3390/bios12020062
ISSN:2079-6374
Parent Title (English):Biosensors
Publisher:MDPI
Document Type:Article
Language:English
Year of Publication:2022
Date of first Publication:2022/01/24
Publishing Institution:Technische Hochschule Wildau
Release Date:2022/02/07
Tag:DNA; cross layer chip; fiber-electrophoresis chip; imprinted electrodes; microfluidic paper analytic device (µPAD); patterning glass microfiber; polymerase chain reaction (PCR); purification
Volume:12
Issue:2
Article Number:62
Source:Heinsohn, N.K.; Niedl, R.R.; Anielski, A.; Lisdat, F.; Beta, C. Electrophoretic µPAD for Purification and Analysis of DNA Samples. Biosensors 2022, 12, 62. https://doi.org/10.3390/bios12020062
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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