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High-throughput cultivation and screening platform for unicellular phototrophs

  • In this work we describe and test high-throughput methods with the model organism Synechocystis sp. PCC6803. The required technical automation for these processes was achieved with a Tecan Freedom Evo 200 pipetting robot. The cultivation was performed in 2.2 ml deepwell microtiter plates within a cultivation chamber outfitted with programmable shaking conditions, variable illumination, variable temperature, and an adjustable CO2 atmosphere. Each microtiter-well within the chamber functions as a separate cultivation vessel with reproducible conditions. The automated measurement of various parameters such as growth, full absorption spectrum, chlorophyll concentration, MALDI-TOF-MS, as well as a novel vitality measurement protocol, have already been established and can be monitored during cultivation. Measurement of growth parameters can be used as inputs for the system to allow for periodic automatic dilutions and therefore a semi-continuous cultivation of hundreds of cultures in parallel. The system also allows the automatic generation of mid and long term backups of cultures to repeat experiments or to retrieve strains of interest.

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Metadaten
Author:Ulrich M. TillichGND, Nick Wolter, Katja Schulze, Dan Kramer, Oliver BrödelORCiD, Marcus FrohmeORCiDGND
URN:urn:nbn:de:kobv:526-opus4-6007
DOI:https://doi.org/10.1186/s12866-014-0239-x
ISSN:1471-2180
Parent Title (English):BMC Microbiology
Document Type:Article
Language:English
Year of Publication:2014
Date of first Publication:2014/09/16
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/06/28
Tag:HTS; automated cultivation; cyanobacteria; high throughput; synechocystis
Volume:14
Issue:239
Page Number:13
Source:Tillich et al.: High-throughput cultivation andscreening platform for unicellular phototrophs. BMC Microbiology 2014 14:239. doi:10.1186/s12866-014-0239-x
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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