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Binding affinity data of DNA aptamers for therapeutic anthracyclines from microscale thermophoresis and surface plasmon resonance spectroscopy

  • Anthracyclines like daunorubicin (DRN) and doxorubicin (DOX) play an undisputed key role in cancer treatment, but their chronic administration can cause severe side effects. For precise anthracycline analytical systems, aptamers are preferable recognition elements. Here, we describe the detailed characterisation of a single-stranded DNA aptamer DRN-10 and its truncated versions for DOX and DRN detection. Binding affinities were determined from surface plasmon resonance (SPR) and microscale thermophoresis (MST) and combined with conformational data from circular dichroism (CD). Both aptamers displayed similar nanomolar binding affinities to DRN and DOX, even though their rate constants differed as shown by SPR recordings. SPR kinetic data unravelled a two-state reaction model including a 1 : 1 binding and a subsequent conformational change of the binding complex. This model was supported by CD spectra. In addition, the dissociation constants determined with MST were always lower than that from SPR, and especially for the truncated aptamer they differed by two orders of magnitude. This most probably reflects the methodological difference, namely labelling for MST vs. immobilisation for SPR. From CD recordings, we suggested a specific G-quadruplex as structural basis for anthracycline binding. We concluded that the aptamer DRN-10 is a promising recognition element for anthracycline detection systems and further selected aptamers can be also characterised with the combined methodological approach presented here.

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Metadaten
Author:Stephan Sass, Walter F. M. Stöcklein, Anja Klevesath, Jeanne Hurpin, Marcus Menger, Carsten HilleORCiD
URN:urn:nbn:de:kobv:526-opus4-16783
DOI (Version of Record):https://doi.org/10.1039/C9AN01247H
Parent Title (English):Analyst
Publisher:Royal Society of Chemistry (RSC)
Document Type:Article
Language:English
Year of Publication:2019
Publishing Institution:Technische Hochschule Wildau
Release Date:2022/11/29
Volume:144
Issue:20
First Page:6064
Last Page:6073
Source:Sass, S., Stöcklein, W., Klevesath, A., Hurpin, J., Menger, M., & Hille, C. (2019). Binding affinity data of DNA aptamers for therapeutic anthracyclines from microscale thermophoresis and surface plasmon resonance spectroscopy. Analyst, The, 144, 6064–6073. doi:10.1039/C9AN01247H
Faculties an central facilities:Zentrale Einrichtungen
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 543 Analytische Chemie
Licence (German):Das Dokument ist urheberrechtlich geschützt
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