Comparison of Sonodynamic Treatment Set-Ups for Cancer Cells with Organic Sonosensitizers and Nanosonosensitizers
- Cancer sonodynamic therapy (SDT) is the therapeutic strategy of a high-frequency ultrasound (US) combined with a special sonosensitizer that becomes cytotoxic upon US exposure. The growing number of newly discovered sonosensitizers and custom US in vitro treatment solutions push the SDT field into a need for systemic studies and reproducible in vitro experimental set-ups. In the current research, we aimed to compare two of the most used and suitable SDT in vitro set-ups—“sealed well” and “transducer in well”—in one systematic study. We assessed US pressure, intensity, and temperature distribution in wells under US irradiation. Treatment efficacy was evaluated for both set-ups towards cancer cell lines of different origins, treated with two promising sonosensitizer candidates—carbon nanoparticle C60 fullerene (C60) and herbal alkaloid berberine. C60 was found to exhibit higher sonotoxicity toward cancer cells than berberine. The higher efficacy of sonodynamic treatment with a “transducer in well” set-up than a “sealed well” set-up underlined its promising application for SDT in vitro studies. The “transducer in well” set-up is recommended for in vitro US treatment investigations based on its US-field homogeneity and pronounced cellular effects. Moreover, SDT with C60 and berberine could be exploited as a promising combinative approach for cancer treatment.
Author: | Aleksandar Radivoievych, Svitlana PrylutskaORCiD, Oliver Zolk, Uwe RitterORCiD, Marcus FrohmeORCiDGND, Anna GrebinykORCiD |
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URN: | urn:nbn:de:kobv:526-opus4-18223 |
DOI (Version of Record): | https://doi.org/10.3390/pharmaceutics15112616 |
ISSN: | 1999-4923 |
Parent Title (English): | Pharmaceutics |
Publisher: | MDPI |
Document Type: | Article |
Language: | English |
Year of Publication: | 2023 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2023/11/14 |
Tag: | C60 fullerene; apoptosis; berberine; sonodynamic therapy; ultrasound |
Volume: | 15 |
Issue: | 11 |
Article Number: | 2616 |
Source: | Radivoievych, A.; Prylutska, S.; Zolk, O.; Ritter, U.; Frohme, M.; Grebinyk, A. Comparison of Sonodynamic Treatment Set-Ups for Cancer Cells with Organic Sonosensitizers and Nanosonosensitizers. Pharmaceutics 2023, 15, 2616. https://doi.org/10.3390/pharmaceutics15112616 |
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Funding: | Publikationsfonds der TH Wildau |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |