Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex
- A nanosized drug complex was explored to improve the efficiency of cancer chemotherapy, complementing it with nanodelivery and photodynamic therapy. For this, nanomolar amounts of a non-covalent nanocomplex of Doxorubicin (Dox) with carbon nanoparticle C60 fullerene (C60) were applied in 1:1 and 2:1 molar ratio, exploiting C60 both as a drug-carrier and as a photosensitizer. The fluorescence microscopy analysis of human leukemic CCRF-CEM cells, in vitro cancer model, treated with nanocomplexes showed Dox’s nuclear and C60’s extranuclear localization. It gave an opportunity to realize a double hit strategy against cancer cells based on Dox’s antiproliferative activity and C60’s photoinduced pro-oxidant activity. When cells were treated with 2:1 C60-Dox and irradiated at 405 nm the high cytotoxicity of photo-irradiated C60-Dox enabled a nanomolar concentration of Dox and C60 to efficiently kill cancer cells in vitro. The high pro-oxidant and pro-apoptotic efficiency decreased IC50 16, 9 and 7 × 103-fold, if compared with the action of Dox, non-irradiated nanocomplex, and C60’s photodynamic effect, correspondingly. Hereafter, a strong synergy of therapy arising from the combination of C60-mediated Dox delivery and C60 photoexcitation was revealed. Our data indicate that a combination of chemo- and photodynamic therapies with C60-Dox nanoformulation provides a promising synergetic approach for cancer treatment.
Author: | Anna GrebinykORCiD, Svitlana PrylutskaORCiD, Oksana Chepurna, Sergii GrebinykORCiD, Yuriy PrylutskyyORCiD, Uwe RitterORCiD, Tymish Y. Ohulchanskyy, Olga Matyshevska, Thomas DandekarORCiDGND, Marcus FrohmeORCiDGND |
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URN: | urn:nbn:de:kobv:526-opus4-12940 |
DOI: | https://doi.org/10.3390/nano9111540 |
ISSN: | 2079-4991 |
Parent Title (English): | Nanomaterials |
Publisher: | MDPI |
Document Type: | Article |
Language: | English |
Year of Publication: | 2019 |
Date of first Publication: | 2019/10/30 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2019/12/18 |
Tag: | C60 fullerene; apoptosis; doxorubicin; leukemic cell; nanocomplex; photodynamic chemotherapy; synergistic effect |
Volume: | 9 |
Issue: | 11 |
Source: | Grebinyk, A., Prylutska, S., Chepurna, O., Grebinyk, S., Prylutskyy, Y., Ritter, U., et al. (2019). Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex Nanomaterials. 9 (11), 1540. |
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Funding: | DFG-geförderter Publikationsfonds |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |