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Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes
- Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.
Author: | Praneeth Reddy Devulapally, Jörg Bürger, Thorsten Mielke, Zoltán Konthur, Hans Lehrach, Marie-Laure Yaspo, Jörn GlöklerORCiD, Hans-Jörg Warnatz |
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URN: | urn:nbn:de:kobv:526-opus4-10538 |
DOI: | https://doi.org/10.1186/s13073-018-0542-5 |
ISSN: | 1756-994X |
Parent Title (English): | Genome Medicine |
Document Type: | Article |
Language: | English |
Year of Publication: | 2018 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2018/10/08 |
Volume: | 10 |
First Page: | 1 |
Last Page: | 12 |
Source: | Devulapally, P., Buerger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M., et al. (2018). Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes Genome Medicine. 10, 1-12. https://doi.org/10.1186/s13073-018-0542-5 |
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |