Re-Emerging Foci of Visceral Leishmaniasis in Armenia – First Molecular Diagnosis of Clinical Samples
- Visceral leishmaniasis (VL) was firstly reported in Armenia in 1913. Following a considerable increase of the number of cases until the mid 1950s, the diseases disappeared after 1969 and re-emerged in 1999. Scientific literature about VL in Armenia is available only in Russian or Armenian. This paper presents a historical overview about leishmaniasis in Armenia based on this literature as well as an epidemiological update since the re-emergence of the disease. In 1999- 2016, 116 indigenous VL cases were recorded mainly in children in 8 of the 11 districts, however VL is underreported because of lack of trained medical personal and diagnostic facilities. The aim of this work was to apply for the first time molecular diagnosis of VL in Armenia. Out of 25 VL suspected patients, 22 were positive by microscopy and PCR. Genotyping using ITS1-PCR-RFLP and sequencing identified the causative agent of VL in Armenia as Leishmania infantum. The present work is an important step towards the inclusion of molecular techniques in the current diagnosis of VL in Armenia and the establishment of local molecular diagnostic facilities.
Author: | Anna Sukiasyan, Ara Keshishyan, Dezdemonia Manukyan, Gayane Melik-Andreasyan, Liana Atshemyan, Hripsime Apresyan, Margarita V. Strelkova, Marcus FrohmeORCiDGND, Sofia CortesORCiD, Katrin KuhlsORCiD |
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URN: | urn:nbn:de:kobv:526-opus4-12018 |
DOI: | https://doi.org/10.1017/S0031182019000064 |
ISSN: | 1469-8161 |
Parent Title (English): | Parasitology |
Document Type: | Article |
Language: | English |
Year of Publication: | 2019 |
Embargo Date: | 2019/08/13 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2019/04/12 |
Page Number: | 20 |
Source: | Sukiasyan, A., Keshishyan, A., Manukyan, D., Melik-Andreasyan, G., Atshemyan, L., Apresyan, H., . . . Kuhls, K. (n.d.). Re-Emerging foci of visceral leishmaniasis in Armenia – first molecular diagnosis of clinical samples. Parasitology, 1-8. doi:10.1017/S0031182019000064 |
Copyright: | This article has been published in a revised form in Parasitology [http://doi.org/10.1017/S0031182019000064]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © Katrin Kuhls. |
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |