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Isothermal amplifications – a comprehensive review on current methods

  • AbstractThe introduction of nucleic acid amplification techniques has revolutionized the field of medical diagnostics in the last decade. The advent of PCR catalyzed the increasing application of DNA, not just for molecular cloning but also for molecular based diagnostics. Since the introduction of PCR, a deeper understanding of molecular mechanisms and enzymes involved in DNA/RNA replication has spurred the development of novel methods devoid of temperature cycling. Isothermal amplification methods have since been introduced utilizing different mechanisms, enzymes, and conditions. The ease with which isothermal amplification methods have allowed nucleic acid amplification to be carried out has had a profound impact on the way molecular diagnostics are being designed after the turn of the millennium. With all the advantages isothermal amplification brings, the issues or complications surrounding each method are heterogeneous making it difficult to identify the best approach for an end-user. This review pays special attention to the various isothermal amplification methods by classifying them based on the mechanistic characteristics which include reaction formats, amplification information, promoter, strand break, and refolding mechanisms. We would also compare the efficiencies and usefulness of each method while highlighting the potential applications and detection methods involved. This review will serve as an overall outlook on the journey and development of isothermal amplification methods as a whole.

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Metadaten
Author:Jörn GlöklerORCiD, Theam Soon Lim, Jeunice Ida, Marcus FrohmeORCiDGND
URN:urn:nbn:de:kobv:526-opus4-15154
DOI (Version of Record):https://doi.org/10.1080/10409238.2021.1937927
Parent Title (English):Critical Reviews in Biochemistry and Molecular Biology
Publisher:Taylor & Francis
Document Type:Article
Language:English
Year of Publication:2021
Publishing Institution:Technische Hochschule Wildau
Release Date:2021/09/15
First Page:1
Last Page:44
Source:Jörn Glökler, Theam Soon Lim, Jeunice Ida & Marcus Frohme (2021) Isothermal amplifications – a comprehensive review on current methods, Critical Reviews in Biochemistry and Molecular Biology, DOI: 10.1080/10409238.2021.1937927
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie
Licence (German):Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International
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