Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer
- G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.
Author: | Noorsharmimi Omar, Qiuting Loh, Gee Jun Tye, Yee Siew Choong, Rahmah Noordin, Jörn GlöklerORCiD, Theam Soon Lim |
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URN: | urn:nbn:de:kobv:526-opus4-6267 |
DOI: | https://doi.org/10.3390/s140100346 |
ISSN: | 1424-8220 |
Parent Title (English): | Sensors |
Document Type: | Article |
Language: | English |
Year of Publication: | 2013 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2016/07/05 |
Tag: | DNAzyme; G-quadruplex; antibody; gold nanoparticle |
Volume: | 14 |
Issue: | 1 |
First Page: | 346 |
Last Page: | 355 |
Source: | Omar, N.; Loh, Q.; Tye, G.J.; Choong, Y.S.; Noordin, R.; Glökler, J.; Lim, T.S. Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer. Sensors 2014, 14, 346-355. |
Faculties an central facilities: | Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014) |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Licence (German): | Creative Commons - CC BY 3.0 - Namensnennung 3.0 Unported |