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Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing

  • During the last decades the ground-breeding Montagu’s harrier ( Circus pygargus , Linnaeus, 1758) has changed its breeding habitats in Europe to agricultural areas in which many local populations would be close to extinction without a special nest protection regime. Although Montagu’s harrier is a well-studied species in terms of ecology and breeding biology, its genetic structure and population genetics are almost unknown. As there is a lack of good genetic markers we developed a set of 19 microsatellite markers comprising 16 new STR markers which were identified by next-generation sequencing (NGS) using 454 shot-gun pyrosequencing of genomic DNA. The STR markers were arranged into three multiplex PCR sets for high throughput genotyping and characterised. The marker set provides a powerful tool for kinship analysis. The combined non-exclusion probability for parent pairs was 1.13* 10-11. Only three loci showed PIC values < 0.50. In total, 121 known family relationships were compared with genetically calculated ones to test the markers suitability for parentage analysis. In 97.5% of all cases full-sibships were accurately determined and 97.6% of all mothers were assigned correctly to their chicks. The present multiplex PCR panels can be used to investigate several hypotheses concerning breeding behaviour, kinship, exchange rates between populations and phylogeography.

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Metadaten
Author:Susann Janowski, Markus GrohmeGND, Marcus FrohmeORCiDGND, Michael Wink
URN:urn:nbn:de:kobv:526-opus4-5966
DOI:https://doi.org/10.2174/1874453201407010011
ISSN:1874-4532
Parent Title (English):The Open Ornithology Journal
Document Type:Article
Language:English
Year of Publication:2014
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/06/28
Tag:Circus pygargus; Dipetalogaster maxima; Montagu’s harrier; genotyping; microsatellites; multiplex PCR; next-generation sequencing; parentage analysis
Volume:7
First Page:11
Last Page:18
Source:Janowski et al. Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun.The Open Ornithology Journal, 2014, 7, 11-18. DOI: 10.2174/1874453201407010011
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY-NC 3.0 - Namensnennung-Nicht kommerziell 3.0 Unported
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