Superoxide Biosensing with Engineered Cytochrome c
- Several mutation positions have been chosen for introducing positively charged lysines in human cytochrome c (cyt c) with the aim of increasing the reaction rate with superoxide radicals (SO) and thus, the sensitivity of an electrochemical cyt c based SO biosensor. The impact of the mutations on structural and redox properties as well as on the reaction rate with SO are verified. Four mutants show a higher reaction rate with the radical compared to the wild type. These mutants are used for the construction of SO sensors based on thiol-modified gold electrodes and covalently fixed proteins. The E66K mutant electrode has a clearly higher sensitivity in comparison to the wildtype based sensor.
Author: | F. Wegerich, Paola Turano, Marco Allegrozzi, H. Möhwald, Fred LisdatORCiDGND |
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URN: | urn:nbn:de:kobv:526-opus4-6523 |
DOI: | https://doi.org/10.1016/j.proche.2009.07.321 |
ISSN: | 1876-6196 |
Parent Title (English): | Procedia Chemistry |
Document Type: | Article |
Language: | English |
Year of Publication: | 2009 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2016/07/12 |
Tag: | biosensor; cytochrome c; protein engineering; superoxide |
Volume: | 1 |
Issue: | 1 |
First Page: | 1287 |
Last Page: | 1290 |
Source: | F. Wegerich et al. Superoxide Biosensing with Engineered Cytochrome c. Procedia Chemistry 1 (2009) 1287–1290. doi:10.1016/j.proche.2009.07.321 |
Faculties an central facilities: | Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014) |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Licence (German): | Creative Commons - CC BY-NC-ND 3.0 - Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Unported |