Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands
- G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands DNA microarray with a fluorescent-labeled G-quadruplex ligand. Among these putative G-quadruplex-forming sequences, G-quadruplex formation was verified for 10 randomly selected sequences by CD spectroscopy and DMS footprinting analysis. In this study, the biological function of the 10 G-quadruplex-forming sequences in the transcriptional regulation has been analyzed using a reporter assay.
Author: | Daniyah H. Bay, Annika Busch, Fred LisdatORCiDGND, Keisuke Iida, Kazunori Ikebukuro, Kazuo Nagasawa, Isao Karube, Wataru Yoshida |
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URN: | urn:nbn:de:kobv:526-opus4-9811 |
DOI: | https://doi.org/10.1186/s12867-017-0094-z |
ISSN: | 1471-2199 |
Parent Title (English): | BMC Molecular Biology |
Document Type: | Article |
Language: | English |
Year of Publication: | 2017 |
Publishing Institution: | Technische Hochschule Wildau |
Release Date: | 2017/08/10 |
Tag: | 7OTD; Cdc6; Dele; G-quadruplex; transcriptional regulation |
Volume: | 18 |
Issue: | 17 |
Source: | Bay et al. BMC Molecular Biol (2017) 18:17. DOI 10.1186/s12867-017-0094-z |
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |