Mohammad Akhoundi, Tim Downing, Jan Votýpka, Katrin Kuhls, Julius Lukeš, Arnaud Cannet, Christophe Ravel, Pierre Marty, Pascal Delaunay, Mohamed Kasbari, Bruno Granouillac, Luigi Gradoni, Denis Sereno
- Progress in the diagnosis of leishmaniases depends on the development of effective methods and the discovery of suitable biomarkers. We propose firstly an update classification of Leishmania species and their synonymies. We demonstrate a global map highlighting the geography of known endemic Leishmania species pathogenic to humans. We summarize a complete list of techniques currently in use and discuss their advantages and limitations. The available data highlights the benefits of molecular markers in terms of their sensitivity and specificity to quantify variation from the subgeneric level to species complexes, (sub) species within complexes, and individual populations and infection foci. Each DNA-based detection method is supplied with a comprehensive description of markers and primers and proposal for a classification based on the role of each target and primer in the detection, identification and quantification of leishmaniasis infection. We outline a genome-wide map of genes informative for diagnosis that have been used for Leishmania genotyping. Furthermore, we propose a classification method based on the suitability of well-studied molecular markers for typing the 21 known Leishmania species pathogenic to humans. This can be applied to newly discovered species and to hybrid strains originating from inter-species crosses. Developing more effective and sensitive diagnostic methods and biomarkers is vital for enhancing Leishmania infection control programs.
MetadatenAuthor: | Mohammad Akhoundi, Tim Downing, Jan Votýpka, Katrin KuhlsORCiD, Julius Lukeš, Arnaud Cannet, Christophe Ravel, Pierre Marty, Pascal Delaunay, Mohamed Kasbari, Bruno Granouillac, Luigi Gradoni, Denis Sereno |
---|
URN: | urn:nbn:de:kobv:526-opus4-9908 |
---|
DOI: | https://doi.org/10.1016/j.mam.2016.11.012 |
---|
ISSN: | 0098-2997 |
---|
Parent Title (English): | Molecular Aspects of Medicine |
---|
Document Type: | Article |
---|
Language: | English |
---|
Year of Publication: | 2017 |
---|
Publishing Institution: | Technische Hochschule Wildau |
---|
Release Date: | 2017/09/15 |
---|
Tag: | diagnostic method; genome-wide map; hybrid strain; molecular marker; sympatric species |
---|
Volume: | 57 |
---|
First Page: | 1 |
---|
Last Page: | 29 |
---|
Source: | M. Akhoundi et al., Leishmania infections: Molecular targets and diagnosis, In Molecular Aspects of Medicine, Volume 57, 2017, Pages 1-29, ISSN 0098-2997, https://doi.org/10.1016/j.mam.2016.11.012. |
---|
Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
---|
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
---|
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
---|