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Integrative Approach to Phlebotomus mascittii Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights

  • Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites Leishmania spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly Phlebotomus mascittii is found, an assumed but unproven vector of Leishmania infantum. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of Ph. mascittii are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European Ph. mascittii populations was made by two marker genes, cytochrome c oxidase subunit 1 (COI), and cytochrome b (cytb), as well as MALDI-TOF mass spectra. Our study provides new important records of Ph. mascittii in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of Ph. mascittii.

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Author:Edwin Kniha, Vít Dvořák, Petr Halada, Markus Milchram, Adelheid G. Obwaller, Katrin KuhlsORCiD, Susanne Schlegel, Martina Köhsler, Wolfgang Poeppl, Karin Bakran-Lebl, Hans-Peter Fuehrer, Věra Volfová, Gerhard Mooseder, Vladimir Ivovic, Petr Volf, Julia Walochnik
URN:urn:nbn:de:kobv:526-opus4-13600
DOI:https://doi.org/10.3390/pathogens9121032
ISSN:2076-0817
Parent Title (English):Pathogens
Publisher:MDPI
Document Type:Article
Language:English
Year of Publication:2020
Date of first Publication:2020/12/09
Publishing Institution:Technische Hochschule Wildau
Release Date:2021/01/08
Tag:Central Europe; MALDI-TOF mass spectrometry; Transphlebotomus; autoimmunofluorescence; genotyping; leishmaniasis
Volume:9
Issue:12
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Creative Commons - CC BY - Namensnennung 4.0 International
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