Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
- Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy.
Download full text files
Additional Services
| Author: | Grit Sandig, Hans Henning von Horsten, Lars Radke, Véronique Blanchard, Marcus FrohmeORCiDGND, Christoph Giese, Volker Sandig, Stephan Hinderlich |
|---|---|
| URN: | urn:nbn:de:kobv:526-opus4-9620 |
| DOI: | https://doi.org/10.3390/bioengineering4020038 |
| ISSN: | 2306-5354 |
| Parent Title (German): | Bioengineering |
| Document Type: | Article |
| Language: | English |
| Year of Publication: | 2017 |
| Publishing Institution: | Technische Hochschule Wildau |
| Release Date: | 2017/05/15 |
| Tag: | CHO; glycoengineering; respiratory syncytial virus; vaccine; xylose |
| Volume: | 4 |
| Issue: | 2 |
| Source: | Sandig, G.; von Horsten, H.H.; Radke, L.; Blanchard, V.; Frohme, M.; Giese, C.; Sandig, V.; Hinderlich, S. Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans. Bioengineering 2017, 4, 38. |
| Faculties an central facilities: | Fachbereich Ingenieur- und Naturwissenschaften |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 66 Chemische Verfahrenstechnik / 660 Chemische Verfahrenstechnik |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
