Superoxide Biosensing with Engineered Cytochrome c

  • Several mutation positions have been chosen for introducing positively charged lysines in human cytochrome c (cyt c) with the aim of increasing the reaction rate with superoxide radicals (SO) and thus, the sensitivity of an electrochemical cyt c based SO biosensor. The impact of the mutations on structural and redox properties as well as on the reaction rate with SO are verified. Four mutants show a higher reaction rate with the radical compared to the wild type. These mutants are used for the construction of SO sensors based on thiol-modified gold electrodes and covalently fixed proteins. The E66K mutant electrode has a clearly higher sensitivity in comparison to the wildtype based sensor.

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Metadaten
Author:F. Wegerich, Paola Turano, Marco Allegrozzi, H. Möhwald, Fred LisdatORCiDGND
URN:urn:nbn:de:kobv:526-opus4-6523
DOI:https://doi.org/10.1016/j.proche.2009.07.321
ISSN:1876-6196
Parent Title (English):Procedia Chemistry
Document Type:Article
Language:English
Year of Publication:2009
Publishing Institution:Technische Hochschule Wildau
Release Date:2016/07/12
Tag:biosensor; cytochrome c; protein engineering; superoxide
Volume:1
Issue:1
First Page:1287
Last Page:1290
Source:F. Wegerich et al. Superoxide Biosensing with Engineered Cytochrome c. Procedia Chemistry 1 (2009) 1287–1290. doi:10.1016/j.proche.2009.07.321
Faculties an central facilities:Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Licence (German):Creative Commons - CC BY-NC-ND 3.0 - Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Unported
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