A New Triple System DNA-Nanosilver-Berberine for Cancer Therapy

  • The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA.In the current work we have investigated the effects of Berberine on the human T-cell leukemia cell line in vitro.Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time-and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction.Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers.The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanopoarticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment.

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Author:Anna GrebinykORCiD, Valeriy Yashchuk, Nataliya Bashmakova, Dmytro Gryn, Tobias Hagemann, Antonina Naumenko, Nataliya Kutsevol, Thomas DandekarORCiDGND, Marcus FrohmeORCiDGND
URN:urn:nbn:de:kobv:526-opus4-10628
DOI:https://doi.org/10.1007/s13204-018-0688-x
ISSN:2190-5517
Parent Title (English):Applied Nanoscience
Document Type:Article
Language:English
Year of Publication:2019
Year of first Publication:2019
Embargo Date:2019/03/03
Publishing Institution:Technische Hochschule Wildau
Release Date:2018/10/22
Tag:Berberine; DNA delivery system; apoptosis; nanosilver
Source:Grebinyk, A., Yashchuk, V., Bashmakova, N., Gryn, D., Hagemann, T., Naumenko, A., et al. (2018). A new triple system DNA-Nanosilver-Berberine for cancer therapy. In Applied Nanoscience. http://dx.doi.org/10.1007/s13204-018-0688-x.
Related Identifier:https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/1610
Faculties an central facilities:Fachbereich Ingenieur- und Naturwissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):Das Dokument ist urheberrechtlich gesch├╝tzt
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