TY - JOUR A1 - Kagel, Heike A1 - Jacobs, Hannes A1 - Bier, Frank Fabian A1 - Glökler, Jörn A1 - Frohme, Marcus T1 - A Novel Microtiter Plate Format High Power Open Source LED Array JF - Photonics N2 - Many photochemical or photobiological applications require the use of high power ultraviolet light sources, such as high-pressure mercury arc lamps. In addition, many photo-induced chemical, biochemical and biological applications require either a combinatorial setting or a parallel assay of multiple samples under the same environmental conditions to ensure reproducibility. To achieve this, alternative, controllable light sources, such as ultraviolet light emitting diodes (UV LEDs) with high power and spatial control are required. Preferably, LEDs are arranged in a suitable standardized 96-well microtiter plate format. We designed such an array and established the methods required for heat management and enabling stable, controllable illumination over time. KW - optics KW - photonics KW - light KW - LED array KW - High-power LED KW - 365 nm KW - microtiter plate Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11307 SN - 2304-6732 VL - 6 IS - 1 ER - TY - JOUR A1 - Kniha, Edwin A1 - Dvořák, Vít A1 - Halada, Petr A1 - Milchram, Markus A1 - Obwaller, Adelheid G. A1 - Kuhls, Katrin A1 - Schlegel, Susanne A1 - Köhsler, Martina A1 - Poeppl, Wolfgang A1 - Bakran-Lebl, Karin A1 - Fuehrer, Hans-Peter A1 - Volfová, Věra A1 - Mooseder, Gerhard A1 - Ivovic, Vladimir A1 - Volf, Petr A1 - Walochnik, Julia T1 - Integrative Approach to Phlebotomus mascittii Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights JF - Pathogens N2 - Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites Leishmania spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly Phlebotomus mascittii is found, an assumed but unproven vector of Leishmania infantum. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of Ph. mascittii are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European Ph. mascittii populations was made by two marker genes, cytochrome c oxidase subunit 1 (COI), and cytochrome b (cytb), as well as MALDI-TOF mass spectra. Our study provides new important records of Ph. mascittii in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of Ph. mascittii. KW - Transphlebotomus KW - Central Europe KW - autoimmunofluorescence KW - MALDI-TOF mass spectrometry KW - genotyping KW - leishmaniasis Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13600 SN - 2076-0817 VL - 9 IS - 12 PB - MDPI ER - TY - JOUR A1 - Demski, Nils Maximilian A1 - Seidlitz, Holger A1 - Kuke, Felix A1 - Dorn, Oliver Niklas A1 - Zoglauer, Janina A1 - Hückstaedt, Tobias A1 - Kamm, Paul Hans A1 - García-Moreno, Francisco A1 - Kremp, Noah A1 - Dreyer, Christian A1 - Oberschmidt, Dirk T1 - Flow-Line-Reducing Tetrahedral Metal Effect Pigments for Injection Molding: A Yield-Rate-Improved Particle Manufacturing Method Based on Soft UVImprint Lithography JF - Polymers N2 - This publication presents an improved manufacturing method for tetrahedral metal effect pigment particles that demonstrates reduced flowlines in injection-molded polymer components compared with conventional platelet-shaped pigment particles. The previously published cold forming process for tetrahedral particles, made entirely from aluminum, faced manufacturing challenges, resulting in a high reject rate due to particle adhesion to the micro-structured mold roller. In contrast, this study introduces a new manufacturing method for tetrahedral particles, now consisting of metallized UV-cured thermoset polymer. These particles, dispersed in amorphous matrix thermoplastics, have shown to maintain their shape during the injection molding process. The manufacturing technique for these novel particles is based on UV imprint lithography, omitting the reject rates compared with the previously presented cold rolling process of tetrahedral full aluminum particles. Thus, the novel manufacturing technique for tetrahedral pigment particles shows increased potential for automation through roll-to-roll manufacturing in the future. KW - flowline KW - injection molding KW - metal effect pigment KW - tetrahedral polymer particle KW - weld line Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20850 SN - 2073-4360 VL - 17 IS - 19 PB - MDPI ER - TY - JOUR A1 - Baleela, Rania A1 - Llewellyn, Martin S. A1 - Fitzpatrick, Sinead A1 - Kuhls, Katrin A1 - Schönian, Gabriele A1 - Miles, Michael A. A1 - Mauricio, Isabel L. T1 - Leishmania donovani populations in Eastern Sudan: temporal structuring and a link between human and canine transmission JF - Parasites & Vectors N2 - Visceral leishmaniasis (VL), caused by the members of the Leishmania donovani complex, has been responsible for devastating VL epidemics in the Sudan. Multilocus microsatellite and sequence typing studies can provide valuable insights into the molecular epidemiology of leishmaniasis, when applied at local scales. Here we present population genetic data for a large panel of strains and clones collected in endemic Sudan between 1993 and 2001. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5921 SN - 1756-3305 VL - 7 IS - 496 ER - TY - JOUR A1 - Tanne, Johannes A1 - Kracher, Daniel A1 - Dietzel, Birgit A1 - Schulz, Burkhard A1 - Ludwig, Roland A1 - Lisdat, Fred A1 - Scheller, Frieder W. A1 - Bier, Frank Fabian T1 - Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes JF - Biosensors N2 - Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose. KW - multiwalled carbon nanotube KW - polyaniline KW - nanohybrids KW - cellobiose dehydrogenase Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5826 SN - 2079-6374 VL - 4 IS - 4 SP - 370 EP - 386 ER - TY - JOUR A1 - Fischbach, Jens A1 - Loh, Qiuting A1 - Bier, Frank Fabian A1 - Lim, Theam Soon A1 - Frohme, Marcus A1 - Glökler, Jörn T1 - Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method JF - Scientific Reports N2 - We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the presence of manganese as quencher and optimize conditions for an online detection. Of the selected dyes, the inexpensive alizarin red S was found to selectively detect pyrophosphate under LAMP and PCR conditions and is superior with respect to its defined red-shifted spectrum, long shelf life and low toxicity. In addition, the newly identified properties may also be useful in other enzymatic assays which do not generate nucleic acids but are based on inorganic pyrophosphate. Finally, we propose that our screening method may provide a blueprint for rapid screening of compounds for detecting inorganic pyrophosphate. KW - biochemical assay KW - fluorescent dye KW - screening Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9248 SN - 2045-2322 VL - 7 ER - TY - JOUR A1 - Rievaj, Vladimir A1 - Stopka, Ondrej A1 - Vrabel, Jan A1 - Mokrickova, Lenka A1 - Schmidt, Conrad T1 - The Impact of Air Resistance on the Fuel Consumption in Real Conditions within the Transport Operation JF - Communications - Scientific Letters of the University of Zilina N2 - This paper presents the impact of air resistance (air resistance) on fuel consumption of the particular vehicle under real traffic conditions. In the introductory and subsequent part of the paper, the individual resistances influencing the moving vehicle are characterized. In the following chapter, the specific factors such as air resistance, driving speed, air temperature and air resistance coefficient in the context of their impact on fuel consumption of the particular vehicle are identified and described; as well as the comparisons of fuel consumption when the vehicle is moving on the highway with closed and open windows under different conditions of road traffic are performed. KW - air resistance KW - vehicles fuel consumption KW - resistance KW - driving speed KW - air temperature KW - air resistance coefficient Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21159 VL - 18 IS - 2 SP - 57 EP - 61 PB - University of Žilina CY - Žilina ER - TY - CHAP A1 - Neumann, Gaby T1 - From human experts to AI agents: Agentic AI for knowledge-intense problem-solving in logistics? T2 - Proceedings of the 26th European Conference on Knowledge Management : VOL 1 N2 - Logistics planning is a complex and complicated problem solving process requiring a wide range of knowledge and competence. A planning person or team represents a particular set of knowledge, skills, experience and personal attitudes. His, her or their level of competence decides about success or failure of the planning and about the quality of its results. With this, logistics planning is knowledge-based and knowledge-creating. Because of this, Knowledge Management can provide tremendous support to the planning person. Even though, there is a wide variety of (software) tools applicable to solve specific sub-problems throughout the planning process, human expertise is the key to develop creative planning solutions so far. Latest developments with regard to Artificial Intelligence (AI) might change this picture. AI is one of the most promising technological advancements, but also one of the most ambivalently discussed topics these days. It has surpassed humans at a number of tasks already and continues in doing so at new tasks in an increasing rate. Because of this, AI is capable to assist humans in many different ways. The latest boost of development moved AI application from those sophisticated communicators or task-oriented AI to intelligent agents or agentic AI allowing for autonomous, goal-driven decision-making. Hybrid approaches combine those advanced AI capabilities with human oversight, offering context-aware adaptability without full autonomy. In this way, artificial intelligence and human intelligence join forces in teams, for example, to solve complex problems creatively. Against this background, the paper analyses agentic AI with regard to its potential to support creative problem solving in a knowledge-intense field like logistics planning. Will AI agents become co-workers in logistics planning teams or can agentic AI design logistics solutions autonomously? With this, the paper focusses on chances and challenges coming with the highly dynamic evolution of AI within a complex, knowledge-intense application area where human experience, problem-solving capability and creativity is decisive. It contributes to discussions about necessary adjustments in how organizations manage their knowledge considering artificial and human intelligence. KW - AI agent KW - agentic AI KW - knowledge management KW - knowledge sharing KW - logistics planning KW - problem-solving Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21223 SP - 719 EP - 727 PB - Academic Conferences and Publishing International CY - Reading ER - TY - JOUR A1 - Popa, Mihaela A1 - Schmidt, Conrad T1 - On the Rail-Based Freight Corridor Between CE and SEE Regions and the Main Obstacles on Romanian Territory JF - Transport Problems N2 - The project “Freight and Logistics Advancement in Central/South-East Europe - Validation of trade and transport processes, Implementation of improvement actions, Application of co-coordinated structures” (in short - FLAVIA) is carried out under the Central Europe – Cooperation for Success Programme, cofunded by the European Regional Development Fund. One of the main objectives of FLAVIA project is to consolidate a logistic corridor from Central Europe (CE) to the South-East Europe (SEE) and the Black Sea Region, based on rail and inland waterways intermodal transport. In this paper we discuss the partial outputs of FLAVIA project, mainly related to the identified actual status of rail-based intermodal transport and trade obstacles of the freight flows on the Romanian territory and the used methodology. Several operational and long-term measures to improve the trade and intermodal transport are listed, considering the advantages of the geo-strategic potential of Romania location, connected with the several improvement directions already considered into the new released intermodal strategy. KW - FLAVIA project KW - intermodal transport corridor KW - European transnational fright flow Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21193 UR - http://transportproblems.polsl.pl/pl/Archiwum/2013/zeszyt1/2013t8z1_05.pdf VL - 8 IS - 1 SP - 47 EP - 56 PB - Silesian University of Technology ER - TY - JOUR A1 - Vrabel, Jan A1 - Stopka, Ondrej A1 - Rievaj, Vladimir A1 - Sarkan, Branislav A1 - Pruskova, Kristyna A1 - Michalk, Philip T1 - Measuring the Resistance of Tires for Passenger Vehicle against the Rolling and Sliding on Loading Area of the Flatbed Truck when Providing the Transport Services JF - Communications - Scientific Letters of the University of Zilina N2 - This paper deals with the size of the forces affecting the vehicle during the transportation on a flatbed truck, railway wagon or container. It is necessary to secure the vehicle against these forces properly in order to prevent vehicles replacement. The laboratory measurements were performed in order to find out the size of coefficient of friction between the tire and the vehicles loading area. During the measurements, vehicle had different gears in order to determine to what extent the size of mechanical resistances affects the fixing and securing the vehicle against its replacement on the loading area. However, this paper does not propose the solution for securing the passenger vehicle on the loading area of a truck, railway wagon or container. KW - mechanical resistance of the vehicle KW - fixing KW - securing KW - loading area KW - sliding friction KW - dynamic coefficient of the sliding friction Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21168 VL - 18 IS - 2 SP - 124 EP - 128 PB - University of Žilina ER - TY - JOUR A1 - Kubanova, Jaroslava A1 - Schmidt, Conrad T1 - Multimodal and Intermodal Transportation Systems JF - Communications - Scientific Letters of the University of Zilina N2 - The aim of this paper is to provide information on the general characterization of multimodal transport, intermodal and combined transport. There is also mentioned the distribution of basic transport processes and types of transhipment. Finally, this paper describes the practical examples of multimodal transport. KW - multimodal transport KW - intermodal transport unit KW - transhipment KW - transport process Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21177 VL - 18 IS - 2 SP - 104 EP - 108 PB - University of Žilina ER -