TY - JOUR A1 - Momanyi, Job A1 - Herzog, Michael A1 - Muchiri, Peter T1 - Analysis of Thermomechanical Properties of Selected Class of Recycled Thermoplastic Materials Based on Their Applications JF - Recycling N2 - Polypropylene and polystyrene are petroleum-based thermoplastics which are commonly used and disposed of in the environment after their service life, leading to environmental degradation. There is a need to recycle polypropylene and polystyrene, but the effect of recycling on thermo-mechanical properties is not well understood. This study aims to determine thermo-mechanical properties of the recycled polypropylene and recycled polystyrene and compare them with corresponding virgin polypropylene and newly produced polystyrene (general purpose polystyrene 1540 and high impact polystyrene 7240). The study was carried out by preparing bar-shaped samples of recycled polypropylene, recycled polystyrene, general purpose polystyrene 1540, and high impact polystyrene 7240 by compression molding using a hot press and thermally characterizing them to determine glass transition temperature and melting temperature using differential scanning calorimetry. The changes in Young’s modulus, tensile strength, hardness, and toughness due to recycling activities were determined at room temperature (24 °C), 40 °C, 60 °C, and 80 °C. The thermo-mechanical properties of recycled polystyrene (PS) were found to be comparable to those of high impact polystyrene (HIPS) 7240. The study revealed that the hardness and toughness for the recycled polymers were higher than those of corresponding virgin polymers. On the other hand, tensile strength and Young's modulus for the recycled polymers were lower than those of the virgin polymers. Understanding the thermo-mechanical properties of the recycled polymers will contribute to more industrial applications hence increase the rate of recycling, resulting in a reduction in environmental pollution. KW - polymer KW - thermoplastic KW - thermo-mechanical property KW - recycled polypropylene KW - recycled polystyrene Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12509 SN - 2313-4321 VL - 4 ER - TY - JOUR A1 - Njuguna, James K. A1 - Muchiri, Peter A1 - Mwema, Fredrick M. A1 - Karuri, Nancy W. A1 - Herzog, Michael A1 - Dimitrov, Kiril T1 - Determination of thermo-mechanical properties of recycled polyurethane from glycolysis polyol JF - Scientific African N2 - Polyurethane foam is one of the most versatile polymers widely used in the automotive industry. However, due to the rising amount of polyurethane foam waste in the environment, there is growing research attention focusing on circular economy solutions to closing the material loop. This study aimed to determine the possible changes in thermo-mechanical properties between rigid polyurethane prepared using polyols derived from depolymerization of commercial polyurethane foam with benchmark rigid polyurethane (Ben PU). Polyurethane foams containing dispersion polyol were reacted with dipropylene glycol (DPG) and diethylene glycol (DEG) with a ratio of DPG: DEG of 1:1 in the presence of a consumable catalyst (Di-n-butyl amine). The recovered polyol was used as a raw material replacing 100% benchmark rigid polyurethane petroleum-based polyester polyol to produce the recycled polyurethane (Rec PU). Thermal analysis was conducted to measure the recycled polyurethane's glass transition temperatures (Tg) using differential scanning calorimetry (DSC). Tensile strength, elastic modulus, toughness, and hardness test of the recycled polyurethane were conducted under three different temperatures; 24°C, 40°C, and 60°C. From the DSC results, the glass transition temperatures for the recycled and the benchmark rigid polyurethane occurred at 43°C and 50.4°C, respectively. Both polymers showed the brittle-ductile transition from 24°C to 40°C. Tensile strength for recycled polyurethane was lower than that of benchmark rigid polyurethane by 29-43% and a corresponding 24-50% decrease in elastic modulus. Recycled polyurethane recorded lower toughness than petroleum-based pure polyurethane by 13-16%. However, the recycled polymer recorded high shored D values than the benchmark rigid polyurethane by 9-29%. This study reveals that recycled polyol could be used as feedstock for polyurethane production with applications tailored to its mechanical properties. KW - polyurethane foam KW - recycling KW - glycolysis KW - rigid polyurethane KW - thermo-mechanical property KW - circular economy Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14911 SN - 2468-2276 VL - 12 ER - TY - JOUR A1 - Reinhardt, Heiner A1 - Mahdaviasl, Mahtab A1 - Prell, Bastian A1 - Mauersberger, Anton A1 - Klimant, Philipp A1 - Reiff-Stephan, Jörg A1 - Ihlenfeldt, Steffen T1 - Efficient data acquisition for traceability and analytics JF - Procedia CIRP N2 - Implementing processes for traceability is required in various industries to assure product quality during manufacturing, provide evidence on required processing conditions or facilitate product recalls. Commonly, radio-frequency identification (RFID) or code recognition techniques (e.g. Data Matrix) are applied to track the flow of workpieces through a manufacturing system and link processing data accordingly. Although the analysis of tracking data is well-examined, we still see a gap in the research on the trade-off between data acquisition, data analytics and data quality. Here, we present a framework to increase the value of existing data by enabling data analytics while addressing common pitfalls and reducing the costs of data management. KW - identification KW - knowledge management KW - manufacturing system KW - process control KW - quality assurance KW - sustainable development KW - decision making KW - complexity KW - analysis KW - design Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-19232 SN - 2212-8271 VL - 122 SP - 73 EP - 78 PB - Elsevier ER - TY - JOUR A1 - Schokraie, Elham A1 - Hotz-Wagenblatt, Agnes A1 - Warnken, Uwe A1 - Mali, Brahim A1 - Frohme, Marcus A1 - Förster, Frank A1 - Dandekar, Thomas A1 - Hengherr, Steffen A1 - Schill, Ralph O. A1 - Schnölzer, Martina T1 - Proteomic Analysis of Tardigrades: Towards a Better Understanding of Molecular Mechanisms by Anhydrobiotic Organisms JF - PLoS ONE N2 - Tardigrades are small, multicellular invertebrates which are able to survive times of unfavourable environmental conditions using their well-known capability to undergo cryptobiosis at any stage of their life cycle. Milnesium tardigradum has become a powerful model system for the analysis of cryptobiosis. While some genetic information is already available for Milnesium tardigradum the proteome is still to be discovered. Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6214 SN - 1932-6203 VL - 5 IS - 3 ER - TY - JOUR A1 - Schokraie, Elham A1 - Warnken, Uwe A1 - Hotz-Wagenblatt, Agnes A1 - Grohme, Markus A1 - Hengherr, Steffen A1 - Förster, Frank A1 - Schill, Ralph O. A1 - Frohme, Marcus A1 - Dandekar, Thomas A1 - Schnölzer, Martina T1 - Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state JF - PLoS ONE N2 - Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state. Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6392 SN - 1932-6203 VL - 7 IS - 9 ER - TY - JOUR A1 - Förster, Frank A1 - Beisser, Daniela A1 - Grohme, Markus A1 - Liang, Chunguang A1 - Mali, Brahim A1 - Siegl, Alexander Matthias A1 - Engelmann, Julia C. A1 - Shkumatov, Alexander A1 - Schokraie, Elham A1 - Müller, Tobias A1 - Schnölzer, Martina A1 - Schill, Ralph O. A1 - Frohme, Marcus A1 - Dandekar, Thomas T1 - Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations JF - Bioinformatics and Biology Insights N2 - Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum. To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions. ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum. These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant. KW - RNA KW - expressed sequence tag KW - cluster KW - protein family KW - adaptation KW - tardigrada KW - transcriptome Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6153 SN - 1177-9322 VL - 2012 IS - 6 SP - 69 EP - 96 ER - TY - JOUR A1 - Förster, Frank A1 - Liang, Chunguang A1 - Shkumatov, Alexander A1 - Beisser, Daniela A1 - Engelmann, Julia C. A1 - Schnölzer, Martina A1 - Frohme, Marcus A1 - Müller, Tobias A1 - Schill, Ralph O. A1 - Dandekar, Thomas T1 - Tardigrade workbench: comparing stress-related proteins, sequence-similar and functional protein clusters as well as RNA elements in tardigrades JF - BMC Genomics N2 - Background Tardigrades represent an animal phylum with extraordinary resistance to environmental stress. Results To gain insights into their stress-specific adaptation potential, major clusters of related and similar proteins are identified, as well as specific functional clusters delineated comparing all tardigrades and individual species (Milnesium tardigradum, Hypsibius dujardini, Echiniscus testudo, Tulinus stephaniae, Richtersius coronifer) and functional elements in tardigrade mRNAs are analysed. We find that 39.3% of the total sequences clustered in 58 clusters of more than 20 proteins. Among these are ten tardigrade specific as well as a number of stress-specific protein clusters. Tardigrade-specific functional adaptations include strong protein, DNA- and redox protection, maintenance and protein recycling. Specific regulatory elements regulate tardigrade mRNA stability such as lox P DICE elements whereas 14 other RNA elements of higher eukaryotes are not found. Further features of tardigrade specific adaption are rapidly identified by sequence and/or pattern search on the web-tool tardigrade analyzer http://waterbear.bioapps.biozentrum.uni-wuerzburg.de. The work-bench offers nucleotide pattern analysis for promotor and regulatory element detection (tardigrade specific; nrdb) as well as rapid COG search for function assignments including species-specific repositories of all analysed data. Conclusion Different protein clusters and regulatory elements implicated in tardigrade stress adaptations are analysed including unpublished tardigrade sequences. Y1 - 2009 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6461 SN - 1471-2164 VL - 10 IS - 469 ER - TY - JOUR A1 - Mali, Brahim A1 - Grohme, Markus A1 - Förster, Frank A1 - Dandekar, Thomas A1 - Schnölzer, Martina A1 - Reuter, Dirk A1 - Wełnicz, Weronika A1 - Schill, Ralph O. A1 - Frohme, Marcus T1 - Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradum in comparison with Hypsibius dujardini and Richtersius coronifer JF - BMC Genomics N2 - The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability of an organism to survive the loss of almost all cellular water without sustaining irreversible damage. Although there are several physiological, morphological and ecological studies on tardigrades, only limited DNA sequence information is available. Therefore, we explored the transcriptome in the active and anhydrobiotic state of the tardigrade Milnesium tardigradum which has extraordinary tolerance to desiccation and freezing. In this study, we present the first overview of the transcriptome of M. tardigradum and its response to desiccation and discuss potential parallels to stress responses in other organisms. Y1 - 2010 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6207 SN - 1471-2164 VL - 11 IS - 168 ER - TY - JOUR A1 - Janowski, Susann A1 - Gross, Ina A1 - Sauer-Gürth, Hedwig A1 - Tietze, Dieter Thomas A1 - Grohme, Markus A1 - Frohme, Marcus A1 - Becker, Peter A1 - Wink, Michael T1 - New Microsatellite Markers for the Common Tern (Sterna hirundo) Developed with 454 Shot-Gun Pyrosequencing JF - The Open Ornithology Journal N2 - Long term studies, focusing on population- and socio-biology research, require the unequivocal identification of individuals. DNA studies with Short Tandem Repeats (STR loci) became a widespread tool in population genetics. We used the next-generation sequencing (NGS) approach with 454 shot-gun pyrosequencing to identify 13 new polymorphic STR loci for the Common Tern, Sterna hirundo. To enlarge the marker set we added two more loci originally developed for Black-legged Kittiwake (Rissa tridactyla) and Red-billed Gull (Chroicocephalus scopulinus) and arranged these 15 loci into three multiplex PCR panels for high throughput genotyping. Loci characterization demonstrated that our marker set is of high quality. A PIC value of about 0.67 and a power of exclusion value of 0.99 were reached. Deviation from Hardy-Weinberg expectations of some loci and low frequencies for null alleles are interpreted as a result of inbreeding and founder effect in the investigated tern colony. We used a test data set of this well-studied breeding colony of Common Tern at Banter Lake, Wilhelmshaven, Germany, to perform a parentage test. Parent-chick relationships, known from the social pedigree of that colony, were compared with genetically calculated ones. In order to test our markers and the used parentage program COLONY, we conducted six competing data sets with varying completeness of included parental genotypes. By including fully sampled parent pairs of known family assignment, results were correct for nest mates, single parents and parent pairs. Our marker set provides a powerful tool to investigate life-time reproductive success and other issues of population and socio-biology for Common Terns, e.g. in the aforementioned colony monitored for decades. KW - common tern KW - COLONY software KW - genotyping KW - microsatellite KW - multiplex PCR KW - next-generation sequencing KW - parentage analysis KW - Sterna hirundo Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6789 SN - 1874-4532 VL - 9 SP - 50 EP - 59 ER - TY - JOUR A1 - Nitsche, Andreas A1 - Kurth, Andreas A1 - Dunkhorst, Anna A1 - Pänke, Oliver A1 - Sielaff, Hendrik A1 - Junge, Wolfgang A1 - Muth, Doreen A1 - Scheller, Frieder W. A1 - Stöcklein, Walter A1 - Dahmen, Claudia A1 - Pauli, Georg A1 - Kage, Andreas T1 - One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX JF - BMC Biotechnology N2 - As a new class of therapeutic and diagnostic reagents, more than fifteen years ago RNA and DNA aptamers were identified as binding molecules to numerous small compounds, proteins and rarely even to complete pathogen particles. Most aptamers were isolated from complex libraries of synthetic nucleic acids by a process termed SELEX based on several selection and amplification steps. Here we report the application of a new one-step selection method (MonoLEX) to acquire high-affinity DNA aptamers binding Vaccinia virus used as a model organism for complex target structures. Y1 - 2007 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6246 SN - 1472-6750 VL - 7 IS - 48 ER - TY - JOUR A1 - Wang, Chong A1 - Grohme, Markus A1 - Mali, Brahim A1 - Schill, Ralph O. A1 - Frohme, Marcus T1 - Towards Decrypting Cryptobiosis—Analyzing Anhydrobiosis in the Tardigrade Milnesium tardigradum Using Transcriptome Sequencing JF - PLoS ONE N2 - Many tardigrade species are capable of anhydrobiosis; however, mechanisms underlying their extreme desiccation resistance remain elusive. This study attempts to quantify the anhydrobiotic transcriptome of the limno-terrestrial tardigrade Milnesium tardigradum. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6029 SN - 1932-6203 VL - 9 IS - 3 ER - TY - JOUR A1 - Grohme, Markus A1 - Mali, Brahim A1 - Wełnicz, Weronika A1 - Michel, Stephanie A1 - Schill, Ralph O. A1 - Frohme, Marcus T1 - The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum JF - Bioinformatics and Biology Insights N2 - Limno-terrestrial tardigrades are small invertebrates that are subjected to periodic drought of their micro-environment. They have evolved to cope with these unfavorable conditions by anhydrobiosis, an ametabolic state of low cellular water. During drying and rehydration, tardigrades go through drastic changes in cellular water content. By our transcriptome sequencing effort of the limno-terrestrial tardigrade Milnesium tardigradum and by a combination of cloning and targeted sequence assembly, we identified transcripts encoding eleven putative aquaporins. Analysis of these sequences proposed 2 classical aquaporins, 8 aquaglyceroporins and a single potentially intracellular unorthodox aquaporin. Using quantitative real-time PCR we analyzed aquaporin transcript expression in the anhydrobiotic context. We have identified additional unorthodox aquaporins in various insect genomes and have identified a novel common conserved structural feature in these proteins. Analysis of the genomic organization of insect aquaporin genes revealed several conserved gene clusters. KW - unorthodox aquaporin KW - anhydrobiosis KW - tardigrada Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6092 SN - 1177-9322 VL - 2013 IS - 7 SP - 153 EP - 165 ER - TY - JOUR A1 - Beisser, Daniela A1 - Grohme, Markus A1 - Kopka, Joachim A1 - Frohme, Marcus A1 - Schill, Ralph O. A1 - Hengherr, Steffen A1 - Dandekar, Thomas A1 - Klau, Gunnar W. A1 - Dittrich, Marcus A1 - Müller, Tobias T1 - Integrated pathway modules using time-course metabolic profiles and EST data from Milnesium tardigradum JF - BMC Systems Biology N2 - Tardigrades are multicellular organisms, resistant to extreme environmental changes such as heat, drought, radiation and freezing. They outlast these conditions in an inactive form (tun) to escape damage to cellular structures and cell death. Tardigrades are apparently able to prevent or repair such damage and are therefore a crucial model organism for stress tolerance. Cultures of the tardigrade Milnesium tardigradum were dehydrated by removing the surrounding water to induce tun formation. During this process and the subsequent rehydration, metabolites were measured in a time series by GC-MS. Additionally expressed sequence tags are available, especially libraries generated from the active and inactive state. The aim of this integrated analysis is to trace changes in tardigrade metabolism and identify pathways responsible for their extreme resistance against physical stress. KW - integrated network analysis KW - functional module KW - metabolic profile KW - metabolic pathway KW - trend test Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6146 SN - 1752-0509 VL - 6 IS - 72 ER - TY - JOUR A1 - Janowski, Susann A1 - Grohme, Markus A1 - Frohme, Marcus A1 - Wink, Michael T1 - Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing JF - The Open Ornithology Journal N2 - During the last decades the ground-breeding Montagu’s harrier ( Circus pygargus , Linnaeus, 1758) has changed its breeding habitats in Europe to agricultural areas in which many local populations would be close to extinction without a special nest protection regime. Although Montagu’s harrier is a well-studied species in terms of ecology and breeding biology, its genetic structure and population genetics are almost unknown. As there is a lack of good genetic markers we developed a set of 19 microsatellite markers comprising 16 new STR markers which were identified by next-generation sequencing (NGS) using 454 shot-gun pyrosequencing of genomic DNA. The STR markers were arranged into three multiplex PCR sets for high throughput genotyping and characterised. The marker set provides a powerful tool for kinship analysis. The combined non-exclusion probability for parent pairs was 1.13* 10-11. Only three loci showed PIC values < 0.50. In total, 121 known family relationships were compared with genetically calculated ones to test the markers suitability for parentage analysis. In 97.5% of all cases full-sibships were accurately determined and 97.6% of all mothers were assigned correctly to their chicks. The present multiplex PCR panels can be used to investigate several hypotheses concerning breeding behaviour, kinship, exchange rates between populations and phylogeography. KW - Circus pygargus KW - Dipetalogaster maxima KW - genotyping KW - microsatellite KW - Montagu’s harrier KW - multiplex PCR KW - next-generation sequencing KW - parentage analysis Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5966 SN - 1874-4532 VL - 7 SP - 11 EP - 18 ER - TY - JOUR A1 - Petrillo, Mauro A1 - Fabbri, Marco A1 - Kagkli, Dafni Maria A1 - Querci, Maddalena A1 - Van den Eede, Guy A1 - Alm, Erik A1 - Aytan-Aktug, Derya A1 - Capella-Gutierrez, Salvador A1 - Carrillo, Catherine A1 - Cestaro, Alessandro A1 - Chan, Kok-Gan A1 - Coque, Teresa A1 - Endrullat, Christoph A1 - Gut, Ivo A1 - Hammer, Paul A1 - Kay, Gemma L. A1 - Madec, Jean-Yves A1 - Mather, Alison E. A1 - McHardy, Alice Carolyn A1 - Naas, Thierry A1 - Paracchini, Valentina A1 - Peter, Silke A1 - Pightling, Arthur A1 - Raffael, Barbara A1 - Rossen, John A1 - Ruppé, Etienne A1 - Schlaberg, Robert A1 - Vanneste, Kevin A1 - Weber, Lukas M. A1 - Westh, Henrik A1 - Angers-Loustau, Alexandre T1 - A roadmap for the generation of benchmarking resources for antimicrobial resistance detection using next generation sequencing [version 1; peer review: 2 approved with reservations] JF - F1000Research N2 - Next Generation Sequencing technologies significantly impact the field of Antimicrobial Resistance (AMR) detection and monitoring, with immediate uses in diagnosis and risk assessment. For this application and in general, considerable challenges remain in demonstrating sufficient trust to act upon the meaningful information produced from raw data, partly because of the reliance on bioinformatics pipelines, which can produce different results and therefore lead to different interpretations. With the constant evolution of the field, it is difficult to identify, harmonise and recommend specific methods for large-scale implementations over time. In this article, we propose to address this challenge through establishing a transparent, performance-based, evaluation approach to provide flexibility in the bioinformatics tools of choice, while demonstrating proficiency in meeting common performance standards. The approach is two-fold: first, a community-driven effort to establish and maintain “live” (dynamic) benchmarking platforms to provide relevant performance metrics, based on different use-cases, that would evolve together with the AMR field; second, agreed and defined datasets to allow the pipelines’ implementation, validation, and quality-control over time. Following previous discussions on the main challenges linked to this approach, we provide concrete recommendations and future steps, related to different aspects of the design of benchmarks, such as the selection and the characteristics of the datasets (quality, choice of pathogens and resistances, etc.), the evaluation criteria of the pipelines, and the way these resources should be deployed in the community. KW - antimicrobial resistance KW - bioinformatics KW - next-generation sequencing KW - benchmarking Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-15699 ER - TY - JOUR A1 - Endrullat, Christoph A1 - Glökler, Jörn A1 - Franke, Philipp A1 - Frohme, Marcus T1 - Standardization and quality management in next-generation sequencing JF - Applied & Translational Genomics N2 - DNA sequencing continues to evolve quickly even after > 30 years. Many new platforms suddenly appeared and former established systems have vanished in almost the same manner. Since establishment of next-generation sequencing devices, this progress gains momentum due to the continually growing demand for higher throughput, lower costs and better quality of data. In consequence of this rapid development, standardized procedures and data formats as well as comprehensive quality management considerations are still scarce. Here, we listed and summarized current standardization efforts and quality management initiatives from companies, organizations and societies in form of published studies and ongoing projects. These comprise on the one hand quality documentation issues like technical notes, accreditation checklists and guidelines for validation of sequencing workflows. On the other hand, general standard proposals and quality metrics are developed and applied to the sequencing workflow steps with the main focus on upstream processes. Finally, certain standard developments for downstream pipeline data handling, processing and storage are discussed in brief. These standardization approaches represent a first basis for continuing work in order to prospectively implement next-generation sequencing in important areas such as clinical diagnostics, where reliable results and fast processing is crucial. Additionally, these efforts will exert a decisive influence on traceability and reproducibility of sequence data. KW - next-generation sequencing KW - quality management KW - data quality KW - standardization KW - validation KW - guideline Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6637 SN - 2212-0661 VL - 10 SP - 2 EP - 9 ER - TY - JOUR A1 - Mohr, Gunther A1 - Nowakowski, Susanna A1 - Altenburg, Simon J. A1 - Maierhofer, Christiane A1 - Hilgenberg, Kai T1 - Experimental Determination of the Emissivity of Powder Layers and Bulk Material in Laser Powder Bed Fusion Using Infrared Thermography and Thermocouples JF - Metals N2 - Recording the temperature distribution of the layer under construction during laser powder bed fusion (L-PBF) is of utmost interest for a deep process understanding as well as for quality assurance and in situ monitoring means. While having a notable number of thermal monitoring approaches in additive manufacturing (AM), attempts at temperature calibration and emissivity determination are relatively rare. This study aims for the experimental temperature adjustment of an off-axis infrared (IR) thermography setup used for in situ thermal data acquisition in L-PBF processes. The temperature adjustment was conducted by means of the so-called contact method using thermocouples at two different surface conditions and two different materials: AISI 316L L-PBF bulk surface, AISI 316L powder surface, and IN718 powder surface. The apparent emissivity values for the particular setup were determined. For the first time, also corrected, closer to real emissivity values of the bulk or powder surface condition are published. In the temperature region from approximately 150 °C to 580 °C, the corrected emissivity was determined in a range from 0.2 to 0.25 for a 316L L-PBF bulk surface, in a range from 0.37 to 0.45 for 316L powder layer, and in a range from 0.37 to 0.4 for IN718 powder layer. KW - laser powder bed fusion (L-PBF) KW - selective laser melting (SLM) KW - laser beam melting (LBM) KW - thermography KW - emissivity KW - calibration KW - thermocouples KW - 316L KW - IN718 KW - process monitoring Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13564 SN - 2075-4701 VL - 10 IS - 11 PB - MDPI ER - TY - JOUR A1 - Ristić, Alenka A1 - Furbo, Simon A1 - Moser, Christoph A1 - Schranzhofer, Hermann A1 - Lazaro, Ana A1 - Delgado, Monica A1 - Peñalosa, Conchita A1 - Zalewski, Laurent A1 - Diarce, Gonzalo A1 - Alkan, Cemil A1 - Gunasekara, Saman N. A1 - Haussmann, Thomas A1 - Gschwander, Stefan A1 - Rathgeber, Christoph A1 - Schmit, Henri A1 - Barreneche, Camila A1 - Cabeza, Luiza A1 - Ferrer, Gerard A1 - Konuklu, Yeliz A1 - Paksoy, Halime A1 - Rammelberg, Holger A1 - Munz, Gunther A1 - Herzog, Thomas H. A1 - Jänchen, Jochen A1 - del Barrio, Elena Palomo T1 - IEA SHC Task 42 / ECES Annex 29 WG A1: Engineering and Processing of PCMs, TCMs and Sorption Materials JF - Energy Procedia N2 - An overview on the recent results on the engineering and characterization of sorption materials, PCMs and TCMs investigated in the working group WG A1 “Engineering and processing of TES materials” of IEA SHC Task 42 / ECES Annex 29 (Task 4229) entitled “Compact Thermal Energy Storage” is presented. KW - engineering KW - processing KW - PCM KW - TCM KW - sorption material Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6629 SN - 1876-6102 VL - 91 SP - 207 EP - 217 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Grynyuk, I. A1 - Kolp, Benjamin A1 - Hurmach, V. A1 - Sliva, T. A1 - Amirkhanov, Volodymyr A1 - Trush, V. A1 - Matyshevska, Olga A1 - Slobodyanik, M. A1 - Prylutskyy, Yuriy A1 - Frohme, Marcus A1 - Ritter, Uwe T1 - C60 Fullerene Effects on Diphenyl-N-(trichloroacetyl)-amidophosphate Interaction with DNA In Silico and Its Cytotoxic Activity Against Human Leukemic Cell Line In Vitro JF - Nanoscale Research Letters N2 - New representative of carbacylamidophosphates - diphenyl-N-(trichloroacetyl)-amidophosphate (HL), which contains two phenoxy substituents near the phosphoryl group, was synthesized, identified by elemental analysis and IR and NMR spectroscopy, and tested as a cytotoxic agent itself and in combination with C60 fullerene. According to molecular simulation results, C60 fullerene and HL could interact with DNA and form a rigid complex stabilized by stacking interactions of HL phenyl groups with C60 fullerene and DNA G nucleotide, as well as by interactions of HL CCl3 group by ion-π bonds with C60 molecule and by electrostatic bonds with DNA G nucleotide. With the use of MTT test, the cytotoxic activity of HL against human leukemic CCRF-CM cells with IC50 value detected at 10 μM concentration at 72 h of cells treatment was shown. Under combined action of 16 μM C60 fullerene and HL, the value of IC50 was detected at lower 5 μM HL concentration and at earlier 48 h period of incubation, besides the cytotoxic effect of HL was observed at a low 2.5 μM concentration at which HL by itself had no influence on cell viability. Binding of C60 fullerene and HL with minor DNA groove with formation of a stable complex is assumed to be one of the possible reasons of their synergistic inhibition of CCRF-CЕM cells proliferation. Application of C60 fullerene in combination with 2.5 μM HL was shown to have no harmful effect on structural stability of blood erythrocytes membrane. Thus, combined action of C60 fullerene and HL in a low concentration potentiated HL cytotoxic effect against human leukemic cells and was not followed by hemolytic effect. KW - diphenyl-N-(trichloroacetyl)-amidophosphate (HL) KW - C60 fullerene KW - leukemic CCRF-CEM cell KW - DNA KW - molecular simulation Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10515 SN - 1556-276X VL - 2018 SP - 1 EP - 9 ER - TY - JOUR A1 - Hollmann, Susanne A1 - Frohme, Marcus A1 - Endrullat, Christoph A1 - Kremer, Andreas A1 - D'Elia, Domenica A1 - Regierer, Babette A1 - Nechyporenko, Alina T1 - Ten simple rules on how to write a standard operating procedure JF - PLoS Computational Biology N2 - Research publications and data nowadays should be publicly available on the internet and, theoretically, usable for everyone to develop further research, products, or services. The long-term accessibility of research data is, therefore, fundamental in the economy of the research production process. However, the availability of data is not sufficient by itself, but also their quality must be verifiable. Measures to ensure reuse and reproducibility need to include the entire research life cycle, from the experimental design to the generation of data, quality control, statistical analysis, interpretation, and validation of the results. Hence, high-quality records, particularly for providing a string of documents for the verifiable origin of data, are essential elements that can act as a certificate for potential users (customers). These records also improve the traceability and transparency of data and processes, therefore, improving the reliability of results. Standards for data acquisition, analysis, and documentation have been fostered in the last decade driven by grassroot initiatives of researchers and organizations such as the Research Data Alliance (RDA). Nevertheless, what is still largely missing in the life science academic research are agreed procedures for complex routine research workflows. Here, well-crafted documentation like standard operating procedures (SOPs) offer clear direction and instructions specifically designed to avoid deviations as an absolute necessity for reproducibility. Therefore, this paper provides a standardized workflow that explains step by step how to write an SOP to be used as a starting point for appropriate research documentation. Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13598 SN - 1553-7358 VL - 16 IS - 9 SP - e1008095 ER - TY - JOUR A1 - Prylutska, Svitlana A1 - Grynyuk, I. A1 - Grebinyk, Anna A1 - Hurmach, V. A1 - Shatrava, Iu. A1 - Sliva, T. A1 - Amirkhanov, Volodymyr A1 - Prylutskyy, Yuriy A1 - Matyshevska, Olga A1 - Slobodyanik, M. A1 - Frohme, Marcus A1 - Ritter, Uwe T1 - Cytotoxic Effects of Dimorfolido-N-Trichloroacetylphosphorylamide and Dimorfolido-N-Benzoylphosphorylamide in Combination with C60 Fullerene on Leukemic Cells and Docking Study of Their Interaction with DNA JF - Nanoscale Research Letters N2 - Dimorfolido-N-trichloroacetylphosphorylamide (HL1) and dimorfolido-N-benzoylphosphorylamide (HL2) as representatives of carbacylamidophosphates were synthesized and identified by the methods of IR, 1H, and 31P NMR spectroscopy. In vitro HL1 and HL2 at 1 mM concentration caused cell specific and time-dependent decrease of leukemic cell viability. Compounds caused the similar gradual decrease of Jurkat cells viability at 72 h (by 35%). HL1 had earlier and more profound toxic effect as compared to HL2 regardless on leukemic cell line. Viability of Molt-16 and CCRF-CEM cells under the action of HL1 was decreased at 24 h (by 32 and 45%, respectively) with no substantial further reducing up to 72 h. Toxic effect of HL2 was detected only at 72 h of incubation of Jurkat and Molt-16 cells (cell viability was decreased by 40 and 45%, respectively). It was shown that C60 fullerene enhanced the toxic effect of HL2 on leukemic cells. Viability of Jurkat and CCRF-CEM cells at combined action of C60 fullerene and HL2 was decreased at 72 h (by 20 and 24%, respectively) in comparison with the effect of HL2 taken separately. In silico study showed that HL1 and HL2 can interact with DNA and form complexes with DNA both separately and in combination with C60 fullerene. More stable complexes are formed when DNA interacts with HL1 or C60 + HL2 structure. Strong stacking interactions can be formed between HL2 and C60 fullerene. Differences in the types of identified bonds and ways of binding can determine distinction in cytotoxic effects of studied compounds. KW - dimorfolido-N-trichloroacetylphosphorylamide KW - dimorfolido-N-benzoylphosphorylamide KW - C60 fullerene KW - leukemic cell KW - DNA KW - computer modeling Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9273 SN - 1556-276X VL - 12 IS - 124 ER - TY - JOUR A1 - Akhoundi, Mohammad A1 - Kuhls, Katrin A1 - Cannet, Arnaud A1 - Votýpka, Jan A1 - Marty, Pierre A1 - Delaunay, Pascal A1 - Sereno, Denis T1 - A Historical Overview of the Classification, Evolution, and Dispersion of Leishmania Parasites and Sandflies JF - PLoS Neglected Tropical Diseases N2 - The aim of this study is to describe the major evolutionary historical events among Leishmania, sandflies, and the associated animal reservoirs in detail, in accordance with the geographical evolution of the Earth, which has not been previously discussed on a large scale. Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5853 SN - 1935-2735 VL - 10 IS - 3 ER - TY - JOUR A1 - Amro, Ahmad A1 - Al-Dwibe, Hamida A1 - Gashout, Aisha A1 - Moskalenko, Olga A1 - Galafin, Marlena A1 - Hamarsheh, Omar A1 - Frohme, Marcus A1 - Jaeschke, Anja A1 - Schönian, Gabriele A1 - Kuhls, Katrin T1 - Spatiotemporal and molecular epidemiology of cutaneous leishmaniasis in Libya JF - PLoS Neglected Tropical Diseases N2 - Cutaneous leishmaniasis (CL) is a skin infection caused by a single-celled parasite that is transmitted by the bite of a phlebotomine sandfly. CL is the most common form of leishmaniasis characterized by localized lesions in the skin and mucous membranes. The disease is prevalent in all countries around the Mediterranean Basin. In this paper, we describe spatiotemporal and eco-epidemiological parameters of CL in Libya. Moreover, we explored current spatiotemporal distributions of CL cases and explored the future projection of the disease. Our study indicates the presence of higher risk of CL in the coastal regions of Libya. Future projection until 2060 showed a trend of increasing incidence of CL in the north-western part of Libya, a spread along the coastal region and a possible emergence of new endemics in the north-eastern districts of Libya. These scenarios should be considered by health authorities in order to develop appropriate intervention strategies and plan effective control programs. Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10054 SN - 1935-2735 VL - 11 IS - 9 ER - TY - JOUR A1 - Kuhls, Katrin A1 - Moskalenko, Olga A1 - Sukiasyan, Anna A1 - Manukyan, Dezdemonia A1 - Melik-Andreasyan, Gayane A1 - Atshemyan, Liana A1 - Apresyan, Hripsime A1 - Strelkova, Margarita V. A1 - Jaeschke, Anja A1 - Wieland, Ralf A1 - Frohme, Marcus A1 - Cortes, Sofia A1 - Keshishyan, Ara T1 - Microsatellite based molecular epidemiology of Leishmania infantum from re-emerging foci of visceral leishmaniasis in Armenia and pilot risk assessment by ecological niche modeling JF - PLoS Neglected Tropical Diseases N2 - Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. In Armenia visceral leishmaniasis (VL) is re-emerging since 1999 after a long break of 30 years, with 167 cases recorded until 2019. Molecular diagnosis of VL was implemented only in 2016, and the causative agent was identified as L. infantum. In the present study we expanded the investigation of the causative agent to a characterization at strain level and the identification of its phylogenetic position among the L. infantum genotypes circulating worldwide. This is the first study addressing genetic diversity and population structure of L. infantum in Armenia and in Transcaucasia. Armenia is an extremely interesting region due to its bio-geographic specificities e.g. the high number of different climates in this small mountainous country and the observed high diversity of sand fly species, part of which occurring in very high altitudes. Ecological niche modeling based on registered VL cases and sand fly vectors collected in active VL foci revealed that the risk of further spread of VL is very high due to climate change. Studies of this region should be expanded to enable targeted control measures. Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14004 SN - 1935-2735 VL - 15 IS - 4 SP - e0009288 PB - Public Library of Science (PLoS) ER - TY - JOUR A1 - Amro, Ahmad A1 - Moskalenko, Olga A1 - Hamarsheh, Omar A1 - Frohme, Marcus T1 - Spatiotemporal analysis of cutaneous leishmaniasis in Palestine and foresight study by projections modelling until 2060 based on climate change prediction JF - PLoS ONE N2 - Background Cutaneous leishmaniasis (CL) is a vector-borne parasitic diseases of public health importance that is prevalent in the West Bank but not in the Gaza Strip. The disease caused by parasitic protozoans from the genus Leishmania and it is transmitted by infected phlebotomine sand flies. The aim of our study is to investigate the eco-epidemiological parameters and spatiotemporal projections of CL in Palestine over a 30-years period from 1990 through 2020 and to explore future projections until 2060. Methodology/Principal findings This long-term descriptive epidemiological study includes investigation of demographic characteristics of reported patients by the Palestinian Ministry of Health (PMoH). Moreover, we explored spatiotemporal distribution of CL including future projection based on climate change scenarios. The number of CL patients reported during this period was 5855 cases, and the average annual incidence rate (AAIR) was 18.5 cases/105 population. The male to female ratio was 1.25:1. Patients-age ranged from 2 months to 89 years (mean = 22.5, std 18.67, and the median was 18 years). More than 65% of the cases came from three governates in the West Bank; Jenin 29% (1617 cases), Jericho 25% (1403), and Tubas 12% (658) with no cases reported in the Gaza Strip. Seasonal occurrence of CL starts to increase in December and peaked during March and April of the following year. Current distribution of CL indicate that Jericho, Tubas, Jenin and Nablus have the most suitable climatic settings for the sandfly vectors. Future projections until 2060 suggest an increasing incidence from northwest of Jenin down to the southwest of Ramallah, disappearance of the foci in Jericho and Tubas throughout the Jordan Vally, and possible emergence of new foci in Gaza Strip. Conclusions/Significance The future projection of CL in Palestine until 2060 show a tendency of increasing incidence in the north western parts of the West Bank, disappearance from Jericho and Tubas throughout the Jordan Vally, and emergence of new CL endemic foci in the Gaza Strip. These results should be considered to implement effective control and surveillance systems to counteract spatial expansion of CL vectors. Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16166 SN - 1932-6203 VL - 17 IS - 6 PB - Public Library of Science (PLoS) ER - TY - JOUR A1 - Marcos-Zambrano, Laura Judith A1 - López-Molina, Víctor Manuel A1 - Bakir-Gungor, Burcu A1 - Frohme, Marcus A1 - Karaduzovic-Hadziabdic, Kanita A1 - Klammsteiner, Thomas A1 - Ibrahimi, Eliana A1 - Lahti, Leo A1 - Loncar-Turukalo, Tatjana A1 - Dhamo, Xhilda A1 - Simeon, Andrea A1 - Nechyporenko, Alina A1 - Pio, Gianvito A1 - Przymus, Piotr A1 - Sampri, Alexia A1 - Trajkovik, Vladimir A1 - Lacruz-Pleguezuelos, Blanca A1 - Aasmets, Oliver A1 - Araujo, Ricardo A1 - Anagnostopoulos, Ioannis A1 - Aydemir, Önder A1 - Berland, Magali A1 - Calle, M. Luz A1 - Ceci, Michelangelo A1 - Duman, Hatice A1 - Gündoğdu, Aycan A1 - Havulinna, Aki S. A1 - Kaka Bra, Kardokh Hama Najib A1 - Kalluci, Eglantina A1 - Karav, Sercan A1 - Lode, Daniel A1 - Lopes, Marta B. A1 - May, Patrick A1 - Nap, Bram A1 - Nedyalkova, Miroslava A1 - Paciência, Inês A1 - Pasic, Lejla A1 - Pujolassos, Meritxell A1 - Shigdel, Rajesh A1 - Susín, Antonio A1 - Thiele, Ines A1 - Truică, Ciprian-Octavian A1 - Wilmes, Paul A1 - Yilmaz, Ercument A1 - Yousef, Malik A1 - Claesson, Marcus Joakim A1 - Truu, Jaak A1 - Carrillo de Santa Pau, Enrique T1 - A toolbox of machine learning software to support microbiome analysis JF - Frontiers in Microbiology N2 - The human microbiome has become an area of intense research due to its potential impact on human health. However, the analysis and interpretation of this data have proven to be challenging due to its complexity and high dimensionality. Machine learning (ML) algorithms can process vast amounts of data to uncover informative patterns and relationships within the data, even with limited prior knowledge. Therefore, there has been a rapid growth in the development of software specifically designed for the analysis and interpretation of microbiome data using ML techniques. These software incorporate a wide range of ML algorithms for clustering, classification, regression, or feature selection, to identify microbial patterns and relationships within the data and generate predictive models. This rapid development with a constant need for new developments and integration of new features require efforts into compile, catalog and classify these tools to create infrastructures and services with easy, transparent, and trustable standards. Here we review the state-of-the-art for ML tools applied in human microbiome studies, performed as part of the COST Action ML4Microbiome activities. This scoping review focuses on ML based software and framework resources currently available for the analysis of microbiome data in humans. The aim is to support microbiologists and biomedical scientists to go deeper into specialized resources that integrate ML techniques and facilitate future benchmarking to create standards for the analysis of microbiome data. The software resources are organized based on the type of analysis they were developed for and the ML techniques they implement. A description of each software with examples of usage is provided including comments about pitfalls and lacks in the usage of software based on ML methods in relation to microbiome data that need to be considered by developers and users. This review represents an extensive compilation to date, offering valuable insights and guidance for researchers interested in leveraging ML approaches for microbiome analysis. KW - microbiome KW - machine learning KW - software KW - feature generation KW - feature analysis KW - data integration KW - microbial gene prediction KW - microbial metabolic modeling Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18271 UR - https://www.frontiersin.org/articles/10.3389/fmicb.2023.1250806/ SN - 1664-302X VL - 14 PB - Frontiers ER - TY - JOUR A1 - Haklay, Muki A1 - Fraisl, Dilek A1 - Greshake Tzovaras, Bastian A1 - Hecker, Susanne A1 - Gold, Margaret A1 - Hager, Gerid A1 - Ceccaroni, Luigi A1 - Kieslinger, Barbara A1 - Wehn, Uta A1 - Woods, Sasha A1 - Nold, Christian A1 - Balázs, Bálint A1 - Mazzonetto, Marzia A1 - Rüfenacht, Simone A1 - Shanley, Lea A. A1 - Wagenknecht, Katherin A1 - Motion, Alice A1 - Sforzi, Andrea A1 - Riemenschneider, Dorte A1 - Dorler, Daniel A1 - Heigl, Florian A1 - Schaefer, Teresa A1 - Lindner, Ariel A1 - Weißpflug, Maike A1 - Mačiulienė, Monika A1 - Vohland, Katrin T1 - Contours of citizen science: a vignette study JF - Royal Society Open Science N2 - Citizen science has expanded rapidly over the past decades. Yet, defining citizen science and its boundaries remained a challenge, and this is reflected in the literature—for example in the proliferation of typologies and definitions. There is a need for identifying areas of agreement and disagreement within the citizen science practitioners community on what should be considered as citizen science activity. This paper describes the development and results of a survey that examined this issue, through the use of vignettes—short case descriptions that describe an activity, while asking the respondents to rate the activity on a scale from ‘not citizen science’ (0%) to ‘citizen science’ (100%). The survey included 50 vignettes, of which five were developed as clear cases of not-citizen science activities, five as widely accepted citizen science activities and the others addressing 10 factors and 61 sub-factors that can lead to controversy about an activity. The survey has attracted 333 respondents, who provided over 5100 ratings. The analysis demonstrates the plurality of understanding of what citizen science is and calls for an open understanding of what activities are included in the field. Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14781 VL - 8 IS - 8 ER - TY - JOUR A1 - Böttcher, René A1 - Hoogland, A. Marije A1 - Dits, Natasja A1 - Verhoef, Esther A1 - Kweldam, Charlotte A1 - Waranecki, Piotr A1 - Bangma, Chris H. A1 - van Leenders, Geert J.L.H. A1 - Jenster, Guido T1 - Novel long non-coding RNAs are specific diagnostic and prognostic markers for prostate cancer JF - Oncotarget N2 - Current prostate cancer (PCa) biomarkers such as PSA are not optimal in distinguishing cancer from benign prostate diseases and predicting disease outcome. To discover additional biomarkers, we investigated PCa-specific expression of novel unannotated transcripts. Using the unique probe design of Affymetrix Human Exon Arrays, we identified 334 candidates (EPCATs), of which 15 were validated by RT-PCR. Combined into a diagnostic panel, 11 EPCATs classified 80% of PCa samples correctly, while maintaining 100% specificity. High specificity was confirmed by in situ hybridization for EPCAT4R966 and EPCAT2F176 (SChLAP1) on extensive tissue microarrays. Besides being diagnostic, EPCAT2F176 and EPCAT4R966 showed significant association with pT-stage and were present in PIN lesions. We also found EPCAT2F176 and EPCAT2R709 to be associated with development of metastases and PCa-related death, and EPCAT2F176 to be enriched in lymph node metastases. Functional significance of expression of 9 EPCATs was investigated by siRNA transfection, revealing that knockdown of 5 different EPCATs impaired growth of LNCaP and 22RV1 PCa cells. Only the minority of EPCATs appear to be controlled by androgen receptor or ERG. Although the underlying transcriptional regulation is not fully understood, the novel PCa-associated transcripts are new diagnostic and prognostic markers with functional relevance to prostate cancer growth. KW - long non-coding RNA KW - prostate cancer KW - in situ hybridization KW - exon array KW - biomarker Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6329 SN - 1949-2553 VL - 6 IS - 6 SP - 4036 EP - 4050 ER - TY - JOUR A1 - Smekhova, Alevtina A1 - Szyjka, Thomas A1 - La Torre, Enrico A1 - Ollefs, Katharina A1 - Eggert, Benedikt A1 - Coester, Birte A1 - Wilhelm, Fabrice A1 - Bali, Rantej A1 - Lindner, Jürgen A1 - Rogalev, Andrei A1 - Többens, Daniel Maria A1 - Weschke, Eugen A1 - Luo, Chen A1 - Chen, Kai A1 - Radu, Florin A1 - Schmitz-Antoniak, Carolin A1 - Wende, Heiko T1 - Irradiation-induced enhancement of Fe and Al magnetic polarizations in Fe60Al40 films JF - New Journal of Physics N2 - The rise of Fe magnetic moment, changes in Al electronic structure and a variation of Al magnetic polarization in thin films of transition metal aluminide Fe60Al40 have been probed through the order-disorder phase transition by soft X-ray absorption spectroscopy and X-ray resonant magnetic reflectivity in the extreme ultraviolet regime. In a course of the transition induced by 20 keV Ne+ irradiation with low fluences (1014 ions·cm-2), X-ray magnetic circular dichroism spectra taken at the Fe L2,3 absorption edges at room and low temperatures revealed a pronounced increase of Fe 3d states spin-polarization. X-ray resonant magnetic reflectivity applied to the Al L2,3 and Fe M2,3 edges allowed to detect the magnetic polarization of Al atoms in the films. The changes in Al electronic structure have been seen by alteration of Al K edge X-ray absorption near edge structure. A difference in anisotropy fields for films before and after irradiation has been observed by element-specific hysteresis loops recorded at low temperatures in absorption and reflection geometries at the Fe L2,3 and M2,3 edges, respectively. An attempt to reduce the top oxide layer by an inductively coupled hydrogen plasma has shown a possibility to recover the chemically ordered phase. KW - chemical disorder KW - ion-irradiation KW - XANES KW - XMCD KW - plasma treatment Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18381 SN - 1367-2630 VL - 26 PB - Institute of Physics Publishing (IOP) ER - TY - JOUR A1 - Kagel, Heike A1 - Bier, Frank Fabian A1 - Frohme, Marcus A1 - Glökler, Jörn T1 - A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids JF - Scientific Reports N2 - Most biochemical reactions depend on the pH value of the aqueous environment and some are strongly favoured to occur in an acidic environment. A non-invasive control of pH to tightly regulate such reactions with defined start and end points is a highly desirable feature in certain applications, but has proven difficult to achieve so far. We report a novel optical approach to reversibly control a typical biochemical reaction by changing the pH and using acid phosphatase as a model enzyme. The reversible photoacid G-acid functions as a proton donor, changing the pH rapidly and reversibly by using high power UV LEDs as an illumination source in our experimental setup. The reaction can be tightly controlled by simply switching the light on and off and should be applicable to a wide range of other enzymatic reactions, thus enabling miniaturization and parallelization through non-invasive optical means. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12685 SN - 2045-2322 VL - 9 ER - TY - JOUR A1 - Kagel, Heike A1 - Jacobs, Hannes A1 - Bier, Frank Fabian A1 - Glökler, Jörn A1 - Frohme, Marcus T1 - A Novel Microtiter Plate Format High Power Open Source LED Array JF - Photonics N2 - Many photochemical or photobiological applications require the use of high power ultraviolet light sources, such as high-pressure mercury arc lamps. In addition, many photo-induced chemical, biochemical and biological applications require either a combinatorial setting or a parallel assay of multiple samples under the same environmental conditions to ensure reproducibility. To achieve this, alternative, controllable light sources, such as ultraviolet light emitting diodes (UV LEDs) with high power and spatial control are required. Preferably, LEDs are arranged in a suitable standardized 96-well microtiter plate format. We designed such an array and established the methods required for heat management and enabling stable, controllable illumination over time. KW - optics KW - photonics KW - light KW - LED array KW - High-power LED KW - 365 nm KW - microtiter plate Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11307 SN - 2304-6732 VL - 6 IS - 1 ER - TY - JOUR A1 - Kniha, Edwin A1 - Dvořák, Vít A1 - Halada, Petr A1 - Milchram, Markus A1 - Obwaller, Adelheid G. A1 - Kuhls, Katrin A1 - Schlegel, Susanne A1 - Köhsler, Martina A1 - Poeppl, Wolfgang A1 - Bakran-Lebl, Karin A1 - Fuehrer, Hans-Peter A1 - Volfová, Věra A1 - Mooseder, Gerhard A1 - Ivovic, Vladimir A1 - Volf, Petr A1 - Walochnik, Julia T1 - Integrative Approach to Phlebotomus mascittii Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights JF - Pathogens N2 - Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites Leishmania spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly Phlebotomus mascittii is found, an assumed but unproven vector of Leishmania infantum. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of Ph. mascittii are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European Ph. mascittii populations was made by two marker genes, cytochrome c oxidase subunit 1 (COI), and cytochrome b (cytb), as well as MALDI-TOF mass spectra. Our study provides new important records of Ph. mascittii in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of Ph. mascittii. KW - Transphlebotomus KW - Central Europe KW - autoimmunofluorescence KW - MALDI-TOF mass spectrometry KW - genotyping KW - leishmaniasis Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13600 SN - 2076-0817 VL - 9 IS - 12 PB - MDPI ER - TY - JOUR A1 - Demski, Nils Maximilian A1 - Seidlitz, Holger A1 - Kuke, Felix A1 - Dorn, Oliver Niklas A1 - Zoglauer, Janina A1 - Hückstaedt, Tobias A1 - Kamm, Paul Hans A1 - García-Moreno, Francisco A1 - Kremp, Noah A1 - Dreyer, Christian A1 - Oberschmidt, Dirk T1 - Flow-Line-Reducing Tetrahedral Metal Effect Pigments for Injection Molding: A Yield-Rate-Improved Particle Manufacturing Method Based on Soft UVImprint Lithography JF - Polymers N2 - This publication presents an improved manufacturing method for tetrahedral metal effect pigment particles that demonstrates reduced flowlines in injection-molded polymer components compared with conventional platelet-shaped pigment particles. The previously published cold forming process for tetrahedral particles, made entirely from aluminum, faced manufacturing challenges, resulting in a high reject rate due to particle adhesion to the micro-structured mold roller. In contrast, this study introduces a new manufacturing method for tetrahedral particles, now consisting of metallized UV-cured thermoset polymer. These particles, dispersed in amorphous matrix thermoplastics, have shown to maintain their shape during the injection molding process. The manufacturing technique for these novel particles is based on UV imprint lithography, omitting the reject rates compared with the previously presented cold rolling process of tetrahedral full aluminum particles. Thus, the novel manufacturing technique for tetrahedral pigment particles shows increased potential for automation through roll-to-roll manufacturing in the future. KW - flowline KW - injection molding KW - metal effect pigment KW - tetrahedral polymer particle KW - weld line Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20850 SN - 2073-4360 VL - 17 IS - 19 PB - MDPI ER - TY - JOUR A1 - Baleela, Rania A1 - Llewellyn, Martin S. A1 - Fitzpatrick, Sinead A1 - Kuhls, Katrin A1 - Schönian, Gabriele A1 - Miles, Michael A. A1 - Mauricio, Isabel L. T1 - Leishmania donovani populations in Eastern Sudan: temporal structuring and a link between human and canine transmission JF - Parasites & Vectors N2 - Visceral leishmaniasis (VL), caused by the members of the Leishmania donovani complex, has been responsible for devastating VL epidemics in the Sudan. Multilocus microsatellite and sequence typing studies can provide valuable insights into the molecular epidemiology of leishmaniasis, when applied at local scales. Here we present population genetic data for a large panel of strains and clones collected in endemic Sudan between 1993 and 2001. Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5921 SN - 1756-3305 VL - 7 IS - 496 ER - TY - JOUR A1 - Tanne, Johannes A1 - Kracher, Daniel A1 - Dietzel, Birgit A1 - Schulz, Burkhard A1 - Ludwig, Roland A1 - Lisdat, Fred A1 - Scheller, Frieder W. A1 - Bier, Frank Fabian T1 - Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes JF - Biosensors N2 - Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose. KW - multiwalled carbon nanotube KW - polyaniline KW - nanohybrids KW - cellobiose dehydrogenase Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5826 SN - 2079-6374 VL - 4 IS - 4 SP - 370 EP - 386 ER - TY - JOUR A1 - Fischbach, Jens A1 - Loh, Qiuting A1 - Bier, Frank Fabian A1 - Lim, Theam Soon A1 - Frohme, Marcus A1 - Glökler, Jörn T1 - Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method JF - Scientific Reports N2 - We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the presence of manganese as quencher and optimize conditions for an online detection. Of the selected dyes, the inexpensive alizarin red S was found to selectively detect pyrophosphate under LAMP and PCR conditions and is superior with respect to its defined red-shifted spectrum, long shelf life and low toxicity. In addition, the newly identified properties may also be useful in other enzymatic assays which do not generate nucleic acids but are based on inorganic pyrophosphate. Finally, we propose that our screening method may provide a blueprint for rapid screening of compounds for detecting inorganic pyrophosphate. KW - biochemical assay KW - fluorescent dye KW - screening Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9248 SN - 2045-2322 VL - 7 ER - TY - JOUR A1 - Rievaj, Vladimir A1 - Stopka, Ondrej A1 - Vrabel, Jan A1 - Mokrickova, Lenka A1 - Schmidt, Conrad T1 - The Impact of Air Resistance on the Fuel Consumption in Real Conditions within the Transport Operation JF - Communications - Scientific Letters of the University of Zilina N2 - This paper presents the impact of air resistance (air resistance) on fuel consumption of the particular vehicle under real traffic conditions. In the introductory and subsequent part of the paper, the individual resistances influencing the moving vehicle are characterized. In the following chapter, the specific factors such as air resistance, driving speed, air temperature and air resistance coefficient in the context of their impact on fuel consumption of the particular vehicle are identified and described; as well as the comparisons of fuel consumption when the vehicle is moving on the highway with closed and open windows under different conditions of road traffic are performed. KW - air resistance KW - vehicles fuel consumption KW - resistance KW - driving speed KW - air temperature KW - air resistance coefficient Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21159 VL - 18 IS - 2 SP - 57 EP - 61 PB - University of Žilina CY - Žilina ER - TY - CHAP A1 - Neumann, Gaby T1 - From human experts to AI agents: Agentic AI for knowledge-intense problem-solving in logistics? T2 - Proceedings of the 26th European Conference on Knowledge Management : VOL 1 N2 - Logistics planning is a complex and complicated problem solving process requiring a wide range of knowledge and competence. A planning person or team represents a particular set of knowledge, skills, experience and personal attitudes. His, her or their level of competence decides about success or failure of the planning and about the quality of its results. With this, logistics planning is knowledge-based and knowledge-creating. Because of this, Knowledge Management can provide tremendous support to the planning person. Even though, there is a wide variety of (software) tools applicable to solve specific sub-problems throughout the planning process, human expertise is the key to develop creative planning solutions so far. Latest developments with regard to Artificial Intelligence (AI) might change this picture. AI is one of the most promising technological advancements, but also one of the most ambivalently discussed topics these days. It has surpassed humans at a number of tasks already and continues in doing so at new tasks in an increasing rate. Because of this, AI is capable to assist humans in many different ways. The latest boost of development moved AI application from those sophisticated communicators or task-oriented AI to intelligent agents or agentic AI allowing for autonomous, goal-driven decision-making. Hybrid approaches combine those advanced AI capabilities with human oversight, offering context-aware adaptability without full autonomy. In this way, artificial intelligence and human intelligence join forces in teams, for example, to solve complex problems creatively. Against this background, the paper analyses agentic AI with regard to its potential to support creative problem solving in a knowledge-intense field like logistics planning. Will AI agents become co-workers in logistics planning teams or can agentic AI design logistics solutions autonomously? With this, the paper focusses on chances and challenges coming with the highly dynamic evolution of AI within a complex, knowledge-intense application area where human experience, problem-solving capability and creativity is decisive. It contributes to discussions about necessary adjustments in how organizations manage their knowledge considering artificial and human intelligence. KW - AI agent KW - agentic AI KW - knowledge management KW - knowledge sharing KW - logistics planning KW - problem-solving Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21223 SP - 719 EP - 727 PB - Academic Conferences and Publishing International CY - Reading ER - TY - JOUR A1 - Popa, Mihaela A1 - Schmidt, Conrad T1 - On the Rail-Based Freight Corridor Between CE and SEE Regions and the Main Obstacles on Romanian Territory JF - Transport Problems N2 - The project “Freight and Logistics Advancement in Central/South-East Europe - Validation of trade and transport processes, Implementation of improvement actions, Application of co-coordinated structures” (in short - FLAVIA) is carried out under the Central Europe – Cooperation for Success Programme, cofunded by the European Regional Development Fund. One of the main objectives of FLAVIA project is to consolidate a logistic corridor from Central Europe (CE) to the South-East Europe (SEE) and the Black Sea Region, based on rail and inland waterways intermodal transport. In this paper we discuss the partial outputs of FLAVIA project, mainly related to the identified actual status of rail-based intermodal transport and trade obstacles of the freight flows on the Romanian territory and the used methodology. Several operational and long-term measures to improve the trade and intermodal transport are listed, considering the advantages of the geo-strategic potential of Romania location, connected with the several improvement directions already considered into the new released intermodal strategy. KW - FLAVIA project KW - intermodal transport corridor KW - European transnational fright flow Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21193 UR - http://transportproblems.polsl.pl/pl/Archiwum/2013/zeszyt1/2013t8z1_05.pdf VL - 8 IS - 1 SP - 47 EP - 56 PB - Silesian University of Technology ER - TY - JOUR A1 - Vrabel, Jan A1 - Stopka, Ondrej A1 - Rievaj, Vladimir A1 - Sarkan, Branislav A1 - Pruskova, Kristyna A1 - Michalk, Philip T1 - Measuring the Resistance of Tires for Passenger Vehicle against the Rolling and Sliding on Loading Area of the Flatbed Truck when Providing the Transport Services JF - Communications - Scientific Letters of the University of Zilina N2 - This paper deals with the size of the forces affecting the vehicle during the transportation on a flatbed truck, railway wagon or container. It is necessary to secure the vehicle against these forces properly in order to prevent vehicles replacement. The laboratory measurements were performed in order to find out the size of coefficient of friction between the tire and the vehicles loading area. During the measurements, vehicle had different gears in order to determine to what extent the size of mechanical resistances affects the fixing and securing the vehicle against its replacement on the loading area. However, this paper does not propose the solution for securing the passenger vehicle on the loading area of a truck, railway wagon or container. KW - mechanical resistance of the vehicle KW - fixing KW - securing KW - loading area KW - sliding friction KW - dynamic coefficient of the sliding friction Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21168 VL - 18 IS - 2 SP - 124 EP - 128 PB - University of Žilina ER - TY - JOUR A1 - Kubanova, Jaroslava A1 - Schmidt, Conrad T1 - Multimodal and Intermodal Transportation Systems JF - Communications - Scientific Letters of the University of Zilina N2 - The aim of this paper is to provide information on the general characterization of multimodal transport, intermodal and combined transport. There is also mentioned the distribution of basic transport processes and types of transhipment. Finally, this paper describes the practical examples of multimodal transport. KW - multimodal transport KW - intermodal transport unit KW - transhipment KW - transport process Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21177 VL - 18 IS - 2 SP - 104 EP - 108 PB - University of Žilina ER - TY - JOUR A1 - Lizbetin, Jan A1 - Vejs, Petr A1 - Caha, Zdenek A1 - Lizbetinova, Lenka A1 - Michalk, Philip T1 - The Possibilities of Dynamic Shipment Weighing in Rail Freight Transport JF - Communications - Scientific Letters of the University of Zilina N2 - This article deals with an analysis of the methods of the dynamic weighing of rail vehicles. The measurement of axle load (both static and dynamic) is one of the current standard processes within the technical specifications for interoperability (TSI). KW - rail transport KW - vehicle weighing KW - interoperability Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21186 VL - 18 IS - 2 SP - 113 EP - 117 PB - University of Žilina ER - TY - JOUR A1 - Lehnert, Martin A1 - Liebchen, Christian A1 - Schiefelbusch, Martin A1 - Bruder, Peter A1 - Harmann, Dennis T1 - Insights into Ridepooling efficiency: A case study analysis using a novel operational performance measure framework JF - Journal of Urban Mobility N2 - The efficiency of demand responsive transport (DRT) services, such as ridepooling, has garnered significant attention across various (simulation) studies. However, a deeper look into the metrics employed to assess system performance exposes notable discrepancies in their utilization. Notably, certain indicators, such as the pooling rate, demonstrate a susceptibility to manipulation based on input parameters, potentially skewing results to give a more favorable impression of service performance. In this paper, we show that, under such circumstances, achieving a fair comparison between study outcomes and with traditional (public) transport modes becomes challenging. In light of these challenges, this study introduces a novel operational performance indicator: Operational System Efficiency (OSE). OSE is tailored to evaluate the operational efficiency of ridepooling systems in a holistic way. In particular, it combines important operational indicators such as detour factor and empty kilometers share, which are considered in numerous analyses. Hereby, OSE is fostering a more equitable assessment of service performance. We applied this methodology and calculated the indicators provided in other studies to two real-world trip datasets from ridepooling services in Berlin and Münster, Germany, and compared the results. Distinct disparities emerge in comparison with conventional efficiency indicators. Consequently, the proposed OSE holds promise for stakeholders, including service providers, public transport companies, and regulatory authorities, as a valuable tool for determining the suitability of a ridepooling service for a given locale relative to other transport modalities, while also providing a transparent assessment of its efficiency. KW - demand responsive transport (DRT) KW - operational system efficiency KW - Pooling index KW - vehicle occupancy Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20971 SN - 2667-0917 VL - 9 PB - Elsevier ER - TY - JOUR A1 - Herkenhoff, Marcos Edgar A1 - Brödel, Oliver A1 - Frohme, Marcus T1 - Hops across Continents: Exploring How Terroir Transforms the Aromatic Profiles of Five Hop (Humulus lupulus) Varieties Grown in Their Countries of Origin and in Brazil JF - Plants N2 - Humulus lupulus, or hops, is a vital ingredient in brewing, contributing bitterness, flavor, and aroma. The female plants produce strobiles rich in essential oils and acids, along with bioactive compounds like polyphenols, humulene, and myrcene, which offer health benefits. This study examined the aromatic profiles of five hop varieties grown in Brazil versus their countries of origin. Fifty grams of pelletized hops from each strain were collected and analyzed using HS-SPME/GC-MS to identify volatile compounds, followed by statistical analysis with PLS-DA and ANOVA. The study identified 330 volatile compounds and found significant aromatic differences among hops from different regions. For instance, H. Mittelfrüher grown in Brazil has a fruity and herbaceous profile, while the German-grown variety is more herbal and spicy. Similar variations were noted in the Magnum, Nugget, Saaz, and Sorachi Ace varieties. The findings underscore the impact of terroir on hop aromatic profiles, with Brazilian-grown hops displaying distinct profiles compared to their counterparts from their countries of origin, including variations in aromatic notes and α-acid content. KW - HS-SPME/GC-MS KW - Humulus lupulus KW - aromatic profile KW - terroir impact KW - volatile compounds Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-19470 VL - 13 IS - 19 ER - TY - JOUR A1 - Herkenhoff, Marcos Edgar A1 - Brödel, Oliver A1 - Dilarri, Guilherme A1 - Bajay, Miklos Maximiliano A1 - Frohme, Marcus A1 - Costamilan, Carlos André da Veiga Lima Rosa T1 - Characterization of Volatile Compounds in Amarillo, Ariana, Cascade, Centennial, and El Dorado Hops Using HS-SPME/GC-MS JF - Compounds N2 - Humulus lupulus L. (hops) is essential in brewing due to its contributions to bitterness, flavor, and aroma. This study compared the volatile profiles of five commercially important hop varieties—Amarillo, Ariana, Cascade, Centennial, and El Dorado—grown in their main regions of origin (United States for Amarillo, Cascade, and El Dorado; Germany for Ariana; and Brazil for Centennial). Headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry (HS-SPME/GC-MS) enabled the identification of 312 volatile compounds, including monoterpenes (e.g., myrcene, linalool, geraniol), sesquiterpenes (e.g., humulene, caryophyllene), esters, alcohols, aldehydes, and ketones. Amarillo showed the highest myrcene content (22.61% of the total volatile area), while Centennial was distinguished by elevated γ-muurolene (20.59%), and El Dorado by the highest level of undecan-2-one (10.47%), highlighting marked varietal differences in key aroma-active constituents. Multivariate, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), clearly discriminated the five varieties: PC1 (41.04% of the variance) separated samples enriched in fruity/floral monoterpenes and esters from those dominated by woody/resinous sesquiterpenes, whereas PC2 (25.93% of the variance) reflected variation in medium-chain esters, ketones, and waxy compounds. These chemometric patterns demonstrate that both genetic background and growing region terroir strongly shape hop volatile composition and, consequently, aroma potential, providing brewers with objective criteria for selecting hop varieties to achieve specific sensory profiles in beer. KW - Humulus lupulus KW - PCA KW - PLS-DA KW - aroma profile KW - brewing science KW - gas chromatography-mass spectrometry KW - hops KW - terroir KW - volatile compounds Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21029 SN - 2673-6918 VL - 6 IS - 1 PB - MDPI ER - TY - JOUR A1 - Ksianzou, Viachaslau A1 - Villringer, Claus A1 - Grytsenko, Kostyantyn A1 - Pekur, Demyd A1 - Lytvyn, Peter A1 - Sopinskyy, Mykola A1 - Lebedyeva, Iryna A1 - Niemczyk, Agata A1 - Baranowska, Jolanta T1 - Structural and Optical Anomalies in Thin Films Grown in a Magnetic Field by Electron-Assisted Vacuum Deposition of PTFE JF - Macromolecular Materials and Engineering N2 - Polytetrafluoroethylene (PTFE) films are deposited in parallel and perpendicular magnetic fields (MF) by electron-enhanced vacuum deposition (EVD) and EVD + low-temperature plasma (LTP) methods. The structure, morphology, and nanomechanical properties of the films are studied by infrared spectroscopy (IRS), atomic force microscopy (AFM), and spectroscopic ellipsometry. The structure of the thicker films is closer to that of bulk PTFE than that of thin films. The films' crystallinity and surface roughness are higher than those deposited without MF. The birefringence of the refractive index (n) of the films deposited in the MF is inverse to the anisotropy of the n of the films deposited without MF. The hardness of the films is close to that of bulk PTFE. KW - birefringence KW - deposition in vacuum KW - morphology KW - plasma KW - thin polytetrafluoroethylene film Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-19712 VL - 310 IS - 3 PB - Wiley ER - TY - JOUR A1 - Sishodia, Keshav A1 - Ben Ltaief, Ltaief A1 - Scheel, Niklas A1 - Földes, István B. A1 - Roos, Andreas Hult A1 - Albrecht, Martin A1 - Staněk, Matyáš A1 - Jurkovičová, Lucie A1 - Hort, Ondrej A1 - Nejdl, Jaroslav A1 - García-Alfonso, Ernesto A1 - Halberstadt, Nadine A1 - Andreasson, Jakob A1 - Klimešová, Eva A1 - Krikunova, Maria A1 - Krishnan, Sivarama A1 - Heidenreich, Andreas A1 - Mudrich, Marcel T1 - XUV fluorescence as a probe of interatomic coulombic decay of resonantly excited He nanodroplets JF - Scientific Reports N2 - Superfluid He nanodroplets resonantly excited by extreme ultraviolet (XUV) pulses can relax via interatomic coulombic decay (ICD), generally considered an ultrafast process. Here, we introduce a novel approach to probe the dynamics of ICD in He nanodroplets over timescales ranging from femtoseconds to nanoseconds. Our method relies on detecting XUV fluorescence emitted from the nanodroplets as they are driven into a nanoplasma by subsequent intense infrared pulses. Nanoplasma ignition is facilitated by tunnel ionization of XUV-excited He∗ atoms attached to the droplets; it thus serves as a sensitive probe of their relaxation dynamics. The observed nanosecond-scale decay is attributed to ICD between pairs of He∗ atoms undergoing roaming motion on the droplet surface, as supported by quantum-mechanical and classical model calculations. Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21050 VL - 16 IS - 1 PB - Springer Nature ER - TY - CHAP A1 - Gross, Matthias A1 - Kuhl, Thorsten A1 - Stephan, Frank A1 - Li, Xiangkun A1 - Philipp, Sebastian A1 - Grebinyk, Anna A1 - Khachatryan, Vitali A1 - Qian, Houjun A1 - Aboulbanine, Zakaria A1 - Amirkhanyan, Zohrab A1 - Riemer, Felix A1 - Oppelt, Anne A1 - Krasilnikov, Mikhail ED - Istituto Nazionale Di Fisca Nucleare, T1 - A beam line setup for flash radiation therapy with focused electron beams at the Pitz facility at DESY in Zeuthen: basic concept and dosimetry simulations T2 - IPAC'23 : 14th International Particle Accelerator Conference, 7-12 May 2023, Venice, Italy : proceedings N2 - The aim of this work is to demonstrate the principal possibility to enhance the electron beam dose deposition in the depth of the sample for radiation therapy purposes. Trains of electron bunches of 22 MeV generated at PITZ are focused inside the sample using a dedicated fast deflector and a solenoid magnet. To explore the capabilities of the proposed setup, dose distributions are calculated for multiple electron bunches focused in a single point inside a water phantom. Electron beam focusing produces dose peaks with a tunable maximal dose depth which is interesting for healthy tissue sparing at the surface and enhancing treatment quality. The duration of the full bunch train is 1 ms. During this time interval, the FLASH effect could be efficiently triggered inside the irradiated target volume. Monte Carlo simulations based on the FLUKA code were performed to evaluate the depth dose curves distributions in a water phantom. Using the PITZ electron beam parameters, simulations have shown the possibility to produce a peak dose in water seven times higher than compared to the dose at the surface. Moreover, the RMS size homogeneous area around the maximal dose is approximately 25 mm. Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21132 SP - 5000 EP - 5003 PB - JACoW Publishing ER - TY - CHAP A1 - Krasilnikov, Mikhail A1 - Aboulbanine, Zakaria A1 - Adhikari, Gowri A1 - Aftab, Namra A1 - Asoyan, Aida A1 - Davtyan, Hakob A1 - Georgiev, Georgi A1 - Good, James A1 - Grebinyk, Anna A1 - Gross, Matthias A1 - Hoffmann, Andreas A1 - Kongmon, Ekkachai A1 - Li, Xiangkun A1 - Lueangaramwong, Anusorn A1 - Melkumyan, David A1 - Mohanty, Sandeep A1 - Niemczyk, Raffael A1 - Oppelt, Anne A1 - Qian, Houjun A1 - Richard, Christopher A1 - Schneidmiller, Evgeny A1 - Stephan, Frank A1 - Vashchenko, Grygorii ED - Istituto Nazionale Di Fisca Nucleare, T1 - THz SASE FEL at PITZ: lasing at a wavelength of 100μm T2 - IPAC'23 : 14th International Particle Accelerator Conference, 7-12 May 2023, Venice, Italy : proceedings N2 - Development of an accelerator-based tunable THz source prototype for pump-probe experiments at the European XFEL is ongoing at the Photo Injector Test facility at DESY in Zeuthen (PITZ). The proof-of-principle experiments on the THz SASE FEL are performed utilizing the LCLS-I undulator installed in the PITZ beamline. The first lasing at a center wavelength of 100 µm was observed in the summer of 2022. The lasing of the narrowband THz source was achieved using an electron beam with an energy of ~17 MeV and a bunch charge up to several nC. Optimization of beam transport and matching resulted in the measurement of THz radiation with a pulse energy of tens of µJ, measured with pyroelectric detectors. The THz FEL gain curves were measured by means of specially designed short coils along the undulator. The results of the first characterization of the THz source at PITZ will be presented. Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21113 SP - 3948 EP - 3951 PB - JACoW Publishing ER - TY - JOUR A1 - Speck, Sandy A1 - Verona, Claudio A1 - Reiff-Stephan, Jörg A1 - Böhme, Andrea A1 - Foitzik, Andreas T1 - Development of a Realistic Brain Phantom for Medical Education: An Ethical and Technical Alternative to Animal Testing JF - Key Engineering Materials N2 - This study presents the development of a realistic brain phantom as a humane, practical alternative to animal use in medical training, in line with the 3R principles and Directive 2010/63/EU. The phantom is designed to support ultrasound-based education for diagnostic and interventional procedures while improving patient safety through controlled, repeatable practice. High‑resolution CT data were converted to CAD models and additively manufactured as moulds; a gelatin‑based soft‑tissue matrix with glycerin and potassium sorbate provides elasticity and durability. Functional features include fluid‑fillable ventricular structures enabling drainage and puncture simulations, and an integrated high‑density tumour insert to model advanced scenarios. Ultrasound evaluation demonstrated clear echogenic differentiation between tumour, ventricles, and surrounding tissue, confirming the model’s imaging fidelity and suitability for hands‑on training. The phantom proved cost‑efficient and reusable, facilitating standardised teaching of complex procedures without ethical approval barriers. A limitation is the material’s gradual mechanical instability under repeated probe contact, motivating investigations into synthetic or hybrid matrices to enhance longevity and reuse. Overall, the brain phantom constitutes an ethical, technically robust, and resource‑efficient training tool that addresses critical needs in healthcare quality and safety by reducing avoidable errors through simulation‑based competence development. KW - 3D printing KW - 3R principle KW - additive manufacturing KW - biomaterial KW - brain phantom KW - ethical alternative KW - medical training KW - phantoms for imaging techniques KW - simulation model KW - ultrasound diagnostics Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21070 VL - 1038 SP - 67 EP - 72 ER - TY - CHAP A1 - Gross, Matthias A1 - Hoffmann, Andreas A1 - Georgiev, Georgi A1 - Kuhl, Thorsten A1 - Li, Xiangkun A1 - Oppelt, Anne A1 - Boonpornprasert, Prach A1 - Philipp, Sebastian A1 - Grebinyk, Anna A1 - Vashchenko, Grygorii A1 - Aftab, Namra A1 - Adhikari, Gowri A1 - Richard, Christopher A1 - Stephan, Frank A1 - Khachatryan, Vitali A1 - Riemer, Felix A1 - Krasilnikov, Mikhail A1 - Amirkhanyan, Zohrab ED - Istituto Nazionale Di Fisca Nucleare, T1 - Comparison of measurements and simulation results of dose for the FLASH radiation therapy beamline at PITZ T2 - IPAC'23 : 14th International Particle Accelerator Conference, 7-12 May 2023, Venice, Italy : proceedings N2 - The high-brightness electron beam at the Photo Injector Test facility at DESY in Zeuthen (PITZ) is now also used for FLASHlab@PITZ: an R&D platform for studying radiation biology and the FLASH effect in radiation therapy. The available parameter space of the electron beam with a momentum of 22 MeV/c allows bunch charges from 10 pC up to 5nC, bunch durations of 0.1–60ps, and bunch train lengths up to 1 ms. The number of bunches in the single train can currently be varied between 1 and 1000 bunches, with an upgrade to 4500 foreseen in 2023. Radiation biology studies require accurate dose prediction, therefore Monte Carlo simulations based on the FLUKA code were performed. According to estimations, dose delivery of 0.002 Gy (low charge case 0.1pC) and 10Gy (high charge case 5nC) is possible, if the beam is confined to a circular area with a radius of 5 mm with a lead collimator. For the Monte Carlo simulations, the experimental setup was accurately modeled, including the exit window, lead collimator, etc. Dose measurements were used to compare simulations with experiments. Dose profiles were experimentally measured with Gafchromic films and then compared with Monte Carlo simulations. The first experiments at FLASHlab@PITZ in 2023 have demonstrated flexible dose options for studying the FLASH effect and radiation biology studies. Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21126 SP - 4996 EP - 4999 PB - JACoW Publishing ER - TY - JOUR A1 - Rogge, Christian A1 - Zinn, Steffen A1 - Prosposito, Paolo A1 - Francini, Roberto A1 - Foitzik, Andreas T1 - Transmitted light pH optode for small sample volumes JF - Journal of Sensors and Sensor Systems N2 - An innovative concept of a low-cost pH optode with working volumes of less than 150 µL is presented. The pH monitoring is based on the color changing effect of pH indicators. The optode includes an RGB color sensor patch TCS34725 from Adafruit, a controllable LED and reactor slides and is addressed by a self-written LabVIEW© software. Utilizing the hue value of the HSV color model, it is possible to analyze the color change of the indicator and estimate the pH value of the analyzed samples by exploiting sigmoidal fit models. Measurements carried out with phenol red and DMEM (Dulbecco's Modified Eagle's Medium) reported a standard error of calibration in the physiologic pH range (6.5–7.5) of ±0.04 pH units. Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10093 SN - 2194-878X VL - 6 IS - 2 SP - 351 EP - 359 ER - TY - JOUR A1 - Kraus, Mirjam A1 - Böhme, Andrea A1 - Rinati, Gianluca Verona T1 - Verification of a Novel Mathematical Model for Determination of the Biomass Specific Growth Rate in Bioprocesses Using Relative Change in Biomass Measurements JF - Defect and Diffusion Forum N2 - This study presents a new mathematical model for determining the specific growth rate of biomass in biotechnological production processes, which aims to optimize the production of biotechnological products such as the advanced material polyhydroxyalkanoates. The specific growth rate is classified by the FDA as a critical process parameter that affects product quality and quantity, but is difficult for laboratory personnel to determine. Therefore, a simple and robust method for real-time monitoring and control is crucial. According to the current state of the art, the established Luedeking-Piret model for determining the specific growth rate requires the determination of the biomass as an absolute value to initialize the model and to determine two further model parameters. However, determining the biomass is time-consuming and error-prone. The new relative model replaces this value with the relative change in biomass, which can be easily recorded using standard laboratory methods such as optical density measurement. This eliminates the need for time-consuming and resource-intensive preliminary work. Despite this simplification, simulation tests have shown that the new model delivers identical results to the established model. It represents an independent, precise alternative and offers advantages in terms of handling. The results underline the model’s potential to make bioprocesses more sustainable and efficient. Especially in research, material consumption, laboratory time and costs can be reduced compared to the established model. Future experiments will further investigate the performance of the new approach compared to the established model. KW - biomass specific growth rate KW - cultivation process KW - off gas KW - oxygen uptake rate KW - real-time monitoring KW - sustainable bioprocess Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21081 VL - 446 SP - 57 EP - 62 PB - Trans Tech Publications ER - TY - JOUR A1 - Roedenbeck, Marc A1 - Herold, Marcel T1 - Personality Profiles in Online Job Advertisements: Applying a Weighted Closed Vocabulary Approach to Identify Generic Profiles and Clusters JF - SAGE Open N2 - On the background of the supplementary applicant-job fit theory, this study tests the application of a weighted closed vocabulary approach as introduced by Ostendorf on a large corpus of online job advertisements (OJA). Therefore, a large dataset of online job advertisements (OJAs) was scraped from a German job portal (N = 151k), and a sample (n = 3,239) was selected based upon Porter’s Value Chain and corresponding job functions. The dataset was pre-processed with techniques of natural language processing, and the predefined words extracted from the OJAs were weighted with the average subject matter expert rating, as offered in the vocabulary of Ostendorf. The results reveal that, first, 0 to 12 words describing personality are used across OJAs, second, that HR, sales, and finance positions show values higher than sample mean (3.22), third, that HR positions show loadings across all dimensions of personality above the sample means (extroversion: 2.32, agreeableness: 3.98, conscientiousness: 4.28, emotional stability: 3.54, openness: 2.0) followed by sales. The job profiles being significantly parallel on equal levels are HR clerk and referee, as well as IT, R&D, and procurement. While conscientiousness is reported to be an important predictor of performance, our study showed only low to average importance in OJAs. KW - LIWC KW - cluster analysis KW - natural language processing KW - online job advertisement KW - personality KW - profile analysis Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21282 VL - 16 IS - 1 PB - Sage ER - TY - JOUR A1 - Shams, S. Fatemeh A1 - Schmitz, Detlef A1 - Smekhova, Alevtina A1 - Ghazanfari, Mohammad Reza A1 - Giesen, Margret A1 - Weschke, Eugen A1 - Chen, Kai A1 - Luo, Chen A1 - Radu, Florin A1 - Schmitz-Antoniak, Carolin T1 - Element-specific contributions to improved magnetic heating of theranostic CoFe2O4 nanoparticles decorated with Pd JF - Scientific Reports N2 - Decoration with Pd clusters increases the magnetic heating ability of cobalt ferrite (CFO) nanoparticles by a factor of two. The origin of this previous finding is unraveled by element-specific X-ray absorption spectroscopy (XAS) and magnetic circular dichroism (XMCD) combined with atomic multiplet simulations and density functional theory (DFT) calculations. While the comparison of XAS spectra with atomic multiplet simulations show that the inversion degree is not affected by Pd decoration and, thus, can be excluded as a reason for the improved heating performance, XMCD reveals two interrelated responsible sources: significantly larger Fe and Co magnetic moments verify an increased total magnetization which enhances the magnetic heating ability. This is accompanied by a remarkable change in the field-dependent magnetization particularly for Co ions which exhibit an increased low-field susceptibility and a reduced spin canting behavior in higher magnetic fields. Using DFT calculations, these findings are explained by reduced superexchange between ions on octahedral lattice sites via oxygen in close vicinity of Pd, which reinforces the dominating antiparallel superexchange interaction between ions on octahedral and tetrahedral lattice sites and thus reduces spin canting. The influence of the delocalized nature of Pd 4d electrons on the neighboring ions is discussed and the conclusions are illustrated with spin density isosurfaces of the involved ions. The presented results pave the way to design nanohybrids with tailored electronic structure and magnetic properties. Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14756 SN - 2045-2322 VL - 11 ER - TY - JOUR A1 - Kalishettyhalli Mahadevaiah, Mamathamba A1 - Pérez, Eduardo A1 - Lisker, Marco A1 - Schubert, Markus Andreas A1 - Perez-Bosch Quesada, Emilio A1 - Wenger, Christian A1 - Mai, Andreas T1 - Modulating the Filamentary-Based Resistive Switching Properties of HfO₂ Memristive Devices by Adding Al₂O₃ Layers JF - Electronics N2 - The resistive switching properties of HfO₂ based 1T-1R memristive devices are electrically modified by adding ultra-thin layers of Al₂O₃ into the memristive device. Three different types of memristive stacks are fabricated in the 130 nm CMOS technology of IHP. The switching properties of the memristive devices are discussed with respect to forming voltages, low resistance state and high resistance state characteristics and their variabilities. The experimental I–V characteristics of set and reset operations are evaluated by using the quantum point contact model. The properties of the conduction filament in the on and off states of the memristive devices are discussed with respect to the model parameters obtained from the QPC fit. Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16172 SN - 2079-9292 VL - 11 IS - 10 CY - MDPI ER - TY - JOUR A1 - Hoogland, A. Marije A1 - Böttcher, René A1 - Verhoef, Esther A1 - Jenster, Guido A1 - van Leenders, Geert J.L.H. T1 - Gene-expression analysis of gleason grade 3 tumor glands embedded in low- and high-risk prostate cancer JF - Oncotarget N2 - The Gleason score (GS) of prostate cancer on diagnostic biopsies is an important parameter for therapeutic decision-making. Biopsy GS under-estimates the actual GS at radical prostatectomy in a significant number of patients due to samplingartifact. The aim of this study was to identify markers that are differentially expressed in Gleason grade 3 (GG3) tumor glands embedded in GS 4 + 3 = 7 and GS 3 + 3 = 6 prostate cancer using laser capture microdissection and RNA sequencing. GG3 tumor glands embedded in nine GS 3 + 3 = 6 and nine GS 4 + 3 = 7 prostate cancers were isolated by laser capture microdissection of frozen radical prostatectomy specimens. After RNA amplification and RNA sequencing, differentially expressed genes in both GG3 components were identified by a 2log fold change > 1.0 and p-value < 0.05. We applied immunohistochemistry on a tissue micro-array representing 481 radical prostatectomy samples for further validation on protein level. A total of 501 genes were up-regulated and 421 down-regulated in GG3 glands embedded in GS 4 + 3 = 7 as compared to GS 3 + 3 = 6 prostate cancer. We selected HELLS, ZIC2 and ZIC5 genes for further validation. ZIC5 mRNA was up-regulated 17 fold (p = 8.4E–07), ZIC2 8 fold (p = 1.3E–05) and HELLS 2 fold (p = 0.006) in GG3 glands derived from GS 4 + 3 = 7. HELLS expression of ≥ 1% occurred in 10% GS < 7, 17% GS 7 and 43% GS >7 prostate cancer (p < 0.001). Using a cut-off of ≥ 1%, protein expression of ZIC5 was present in 28% GS < 7, 43% GS 7 and 57% GS > 7 cancer (p < 0.001). ZIC2 was neither associated with GS nor outcome in our validation set. HELLS was independently predictive for biochemical-recurrence after radical prostatectomy (HR 2.3; CI 1.5–3.6; p < 0.01). In conclusion, HELLS and ZIC5 might be promising candidate markers for selection of biopsy GS 6 prostate cancer being at risk for up-grading at prostatectomy. KW - prostate cancer KW - RNA sequencing KW - laser capture microdissection KW - immunohistochemistry Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6573 SN - 1949-2553 VL - 7 IS - 25 SP - 37846 EP - 37856 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Buchelnikov, Anatoliy A1 - Tverdokhleb, Nina A1 - Grebinyk, Sergii A1 - Evstigneev, Maxim A1 - Matyshevska, Olga A1 - Cherepanov, Vsevolod A1 - Prylutskyy, Yuriy A1 - Yashchuk, Valeriy A1 - Naumovets, Anton A1 - Ritter, Uwe A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells JF - Pharmaceutics N2 - A herbal alkaloid Berberine (Ber), used for centuries in Ayurvedic, Chinese, Middle-Eastern, and native American folk medicines, is nowadays proved to function as a safe anticancer agent. Yet, its poor water solubility, stability, and bioavailability hinder clinical application. In this study, we have explored a nanosized carbon nanoparticle—C60 fullerene (C60)—for optimized Ber delivery into leukemic cells. Water dispersions of noncovalent C60-Ber nanocomplexes in the 1:2, 1:1, and 2:1 molar ratios were prepared. UV–Vis spectroscopy, dynamic light scattering (DLS), and atomic force microscopy (AFM) evidenced a complexation of the Ber cation with the negatively charged C60 molecule. The computer simulation showed that π-stacking dominates in Ber and C60 binding in an aqueous solution. Complexation with C60 was found to promote Ber intracellular uptake. By increasing C60 concentration, the C60-Ber nanocomplexes exhibited higher antiproliferative potential towards CCRF-CEM cells, in accordance with the following order: free Ber < 1:2 < 1:1 < 2:1 (the most toxic). The activation of caspase 3/7 and accumulation in the sub-G1 phase of CCRF-CEM cells treated with C60-Ber nanocomplexes evidenced apoptosis induction. Thus, this study indicates that the fast and easy noncovalent complexation of alkaloid Ber with C60 improved its in vitro efficiency against cancer cells. KW - C60 fullerene KW - berberine KW - noncovalent nanocomplex KW - UV–Vis KW - DLS and AFM measurements KW - drug release KW - leukemic cell KW - uptake KW - cytotoxicity KW - apoptosis Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12932 SN - 1999-4923 VL - 11 IS - 11 PB - MDPI ER - TY - JOUR A1 - Köberlein, Jana A1 - Bank, Lukas A1 - Roth, Stefan A1 - Köse, Ekrem A1 - Kuhlmann, Timm A1 - Prell, Bastian A1 - Stange, Maximilian A1 - Münnich, Marc A1 - Flum, Dominik A1 - Moog, Daniel A1 - Ihlenfeldt, Steffen A1 - Sauer, Alexander A1 - Weigold, Matthias A1 - Schilp, Johannes T1 - Simulation Modeling for Energy-Flexible Manufacturing: Pitfalls and How to Avoid Them JF - Energies N2 - Due to the high share of industry in total electricity consumption, industrial demand-side management can make a relevant contribution to the stability of power systems. At the same time, companies get the opportunity to reduce their electricity procurement costs by taking advantage of increasingly fluctuating prices on short-term electricity markets, the provision of system services on balancing power markets, or by increasing the share of their own consumption from on-site generated renewable energy. Demand-side management requires the ability to react flexibly to the power supply situation without negatively affecting production targets. It also means that the management and operation of production must consider not only production-related parameters but also parameters of energy availability, which further increase the complexity of decision-making. Although simulation studies are a recognized tool for supporting decision-making processes in production and logistics, the simultaneous simulation of material and energy flows has so far been limited mainly to issues of energy efficiency as opposed to energy flexibility, where application-oriented experience is still limited. We assume that the consideration of energy flexibility in the simulation of manufacturing systems will amplify already known pitfalls in conducting simulation studies. Based on five representative industrial use cases, this article provides practitioners with application-oriented experiences of the coupling of energy and material flows in simulation modeling of energy-flexible manufacturing, identifies challenges in the simulation of energy-flexible production systems, and proposes approaches to face these challenges. Seven pitfalls that pose a particular challenge in simulating energy-flexible manufacturing have been identified, and possible solutions and measures for avoiding them are shown. It has been found that, among other things, consistent management of all parties involved, early clarification of energy-related, logistical, and resulting technical requirements for models and software, as well as the application of suitable methods for validation and verification are central to avoiding these pitfalls. The identification and characterization of challenges and the derivation of recommendations for coping with them can raise awareness of typical pitfalls. This paper thus helps to ensure that simulation studies of energy-flexible production systems can be carried out more efficiently in the future. KW - industrial demand-side management KW - energy-flexible manufacturing system KW - simulation pitfall KW - simulation of material and energy flows Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16152 SN - 1996-1073 VL - 15 IS - 10 PB - MDPI ER - TY - JOUR A1 - Kölsch, Adrian A1 - Radon, C. A1 - Golub, M. A1 - Baumert, A. A1 - Bürger, Jörg A1 - Mielke, Thorsten A1 - Lisdat, Fred A1 - Feoktystov, Artem A1 - Pieper, Jörg A1 - Zouni, Athina A1 - Wendler, Petra T1 - Current limits of structural biology: The transient interaction between cytochrome c6 and photosystem I JF - Current Research in Structural Biology N2 - Trimeric photosystem I from the cyanobacterium Thermosynechococcus elongatus (TePSI) is an intrinsic membrane protein, which converts solar energy into electrical energy by oxidizing the soluble redox mediator cytochrome c6 (Cyt c6) and reducing ferredoxin. Here, we use cryo-electron microscopy and small angle neutron scattering (SANS) to characterize the transient binding of Cyt c6 to TePSI. The structure of TePSI cross-linked to Cyt c6 was solved at a resolution of 2.9 Å and shows additional cofactors as well as side chain density for 84% of the peptide chain of subunit PsaK, revealing a hydrophobic, membrane intrinsic loop that enables binding of associated proteins. Due to the poor binding specificity, Cyt c6 could not be localized with certainty in our cryo-EM analysis. SANS measurements confirm that Cyt c6 does not bind to TePSI at protein concentrations comparable to those for cross-linking. However, SANS data indicate a complex formation between TePSI and the non-native mitochondrial cytochrome from horse heart (Cyt cHH). Our study pinpoints the difficulty of identifying very small binding partners (less than 5% of the overall size) in EM structures when binding affinities are poor. We relate our results to well resolved co-structures with known binding affinities and recommend confirmatory methods for complexes with KM values higher than 20 μM. KW - photosystem I KW - cryo-EM KW - Thermosynechococcus elongatus KW - small angle neutron scattering KW - cytochrome c6 KW - electron transfer KW - photo-biotechnology Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13628 SN - 2665-928X VL - 2 SP - 171 EP - 179 ER - TY - JOUR A1 - Devulapally, Praneeth Reddy A1 - Bürger, Jörg A1 - Mielke, Thorsten A1 - Konthur, Zoltán A1 - Lehrach, Hans A1 - Yaspo, Marie-Laure A1 - Glökler, Jörn A1 - Warnatz, Hans-Jörg T1 - Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes JF - Genome Medicine N2 - Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies. Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10538 SN - 1756-994X VL - 10 SP - 1 EP - 12 ER - TY - JOUR A1 - Baumann, Elisabeth A1 - Pohle, Ulrike A1 - Zhang, Edward A1 - Allen, Thomas A1 - Villringer, Claus A1 - Pulwer, Silvio A1 - Gerhardt, Holger A1 - Laufer, Jan T1 - A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor JF - Photoacoustics N2 - Optical-resolution photoacoustic microscopy (OR-PAM) combines high spatial resolution and strong absorption-based contrast in tissue, which has enabled structural and spectroscopic imaging of endogenous chromophores, primarily hemoglobin. Conventional piezoelectric ultrasound transducers are typically placed far away from the photoacoustic source due to their opacity, which reduces acoustic sensitivity. Optical ultrasound sensors are an alternative as their transparency allows them to be positioned close to the sample with minimal source-detector distances. In this work, a backward-mode OR-PAM system based on a planar Fabry-Pérot ultrasound sensor and coaxially aligned excitation and interrogation beams was developed. Two 3D imaging modes, using raster-scanning for enhanced image quality and continuous-scanning for fast imaging, were implemented and tested on a leaf skeleton phantom. In fast imaging mode, a scan-rate of 100,000 A-lines/s was achieved. 3D images of a zebrafish embryo were acquired in vivo in raster-scanning mode. The transparency of the FP sensor in the visible and near-infrared wavelength region makes it suitable for combined functional and molecular imaging applications using OR-PAM and multi-photon fluorescence microscopy. KW - optical-resolution photoacoustic microscopy KW - backward-mode imaging KW - dual-wavelength KW - continuous scanning KW - in vivo imaging KW - optical ultrasound sensing KW - planar Fabry-Pérot interferometer Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14701 SN - 2213-5979 VL - 24 SP - 100293 PB - Elsevier ER - TY - JOUR A1 - Stumper, Nele A1 - Berger, Jörn A1 - Klotsche, Jens A1 - Gedat, Egbert A1 - Hoff, Paula A1 - Schmittat, Gabriela A1 - Burmester, Gerd-Rüdiger A1 - Krönke, Gerhard A1 - Backhaus, Marina A1 - Haugen, Ida Kristin A1 - Ohrndorf, Sarah T1 - To optimise the diagnostic process of rheumatic diseases affecting the hands using fluorescence optical imaging (FOI) JF - RMD Open N2 - Background Accurate and rapid diagnosis of rheumatic diseases is essential for further treatment decision. Different rheumatic diseases present characteristic patterns (image features) in fluorescence optical imaging (FOI). We developed an atlas of FOI image features and tested its ability to differentiate various rheumatic diseases. Methods FOI images from patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), connective tissue diseases (CTD) and osteoarthritis (OA) were analysed by two readers blinded for diagnosis and calibrated against each other, using the prima vista mode (PVM) and an automated 5-phase model. Twenty-six different reoccurring typical signal enhancement patterns (features) indicating inflamed joints, nail or skin were defined and all FOI images were scored accordingly. The feature frequency in each patient cohort and phase (PVM, 5-phase) was counted. Contingency tables were created with categorical variable counts and diagnosis using common formulae. Findings Four hundred thirty-eight patients with RA (n=117), PsA (n=110), CTD (n=121) and OA (n=90) were included. Once the data had been categorised, a two-step diagnostic pathway was developed: in the first step, OA was best distinguished from the other diseases with high specificity by five patterns (specificity >0.9, diagnostic OR between 2.34 and 8.24). In a second step, the remaining autoimmune diseases were differentiated from each other by a certain number of features (five for RA, 12 for PsA and four for CTD). Interpretation This was the first study to show that feature analysis in FOI helps to differentiate typical rheumatic diseases from each other, potentially simplifying and speeding up the diagnostic process. Therefore, FOI could be considered an additional component of a wider range of imaging techniques used in rheumatology. Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20698 VL - 11 IS - 3 PB - EULAR ER - TY - JOUR A1 - Gedat, Egbert A1 - Berger, Jörn A1 - Kiesel, Denise A1 - Failli, Vieri A1 - Briel, Andreas A1 - Welker, Pia T1 - Features Found in Indocyanine Green-Based Fluorescence Optical Imaging of Inflammatory Diseases of the Hands JF - Diagnostics N2 - Rheumatologists in Europe and the USA increasingly rely on fluorescence optical imaging (FOI, Xiralite) for the diagnosis of inflammatory diseases. Those include rheumatoid arthritis, psoriatic arthritis, and osteoarthritis, among others. Indocyanine green (ICG)-based FOI allows visualization of impaired microcirculation caused by inflammation in both hands in one examination. Thousands of patients are now documented and most literature focuses on inflammatory arthritides, which affect synovial joints and their related structures, making it a powerful tool in the diagnostic process of early undifferentiated arthritis and rheumatoid arthritis. However, it has become gradually clear that this technique has the potential to go even further than that. FOI allows visualization of other types of tissues. This means that FOI can also support the diagnostic process of vasculopathies, myositis, collagenoses, and other connective tissue diseases. This work summarizes the most prominent imaging features found in FOI examinations of inflammatory diseases, outlines the underlying anatomical structures, and introduces a nomenclature for the features and, thus, supports the idea that this tool is a useful part of the imaging repertoire in rheumatology clinical practice, particularly where other imaging methods are not easily available. KW - optical diagnosis imaging KW - fluorescence optical imaging KW - indocyanine green fluorescence KW - image feature KW - image analysis KW - rheumatoid disease Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16359 SN - 2075-4418 VL - 12 IS - 8 PB - MDPI ER - TY - JOUR A1 - Bay, Daniyah H. A1 - Busch, Annika A1 - Lisdat, Fred A1 - Iida, Keisuke A1 - Ikebukuro, Kazunori A1 - Nagasawa, Kazuo A1 - Karube, Isao A1 - Yoshida, Wataru T1 - Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands JF - BMC Molecular Biology N2 - G-quadruplex is a DNA secondary structure that has been shown to play an important role in biological systems. In a previous study, we identified 1998 G-quadruplex-forming sequences using a mouse CpG islands DNA microarray with a fluorescent-labeled G-quadruplex ligand. Among these putative G-quadruplex-forming sequences, G-quadruplex formation was verified for 10 randomly selected sequences by CD spectroscopy and DMS footprinting analysis. In this study, the biological function of the 10 G-quadruplex-forming sequences in the transcriptional regulation has been analyzed using a reporter assay. KW - Cdc6 KW - Dele KW - G-quadruplex KW - transcriptional regulation KW - 7OTD Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9811 SN - 1471-2199 VL - 18 IS - 17 ER - TY - JOUR A1 - Belcheva, Veronika A1 - Ermakova, Tatiana A1 - Fabian, Benjamin T1 - Understanding Website Privacy Policies—A Longitudinal Analysis Using Natural Language Processing JF - Information N2 - Privacy policies are the main method for informing Internet users of how their data are collected and shared. This study aims to analyze the deficiencies of privacy policies in terms of readability, vague statements, and the use of pacifying phrases concerning privacy. This represents the undertaking of a step forward in the literature on this topic through a comprehensive analysis encompassing both time and website coverage. It characterizes trends across website categories, top-level domains, and popularity ranks. Furthermore, studying the development in the context of the General Data Protection Regulation (GDPR) offers insights into the impact of regulations on policy comprehensibility. The findings reveal a concerning trend: privacy policies have grown longer and more ambiguous, making it challenging for users to comprehend them. Notably, there is an increased proportion of vague statements, while clear statements have seen a decrease. Despite this, the study highlights a steady rise in the inclusion of reassuring statements aimed at alleviating readers’ privacy concerns. KW - privacy policy KW - longitudinal analysis KW - text analysis KW - NLP KW - readability KW - vagueness Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18268 SN - 2078-2489 VL - 14 IS - 11 PB - MDPI ER - TY - JOUR A1 - Rothe, Felix A1 - Berger, Jörn A1 - Welker, Pia A1 - Fiebelkorn, Richard A1 - Kupper, Stefan A1 - Kiesel, Denise A1 - Gedat, Egbert A1 - Ohrndorf, Sarah T1 - Fluorescence optical imaging feature selection with machine learning for differential diagnosis of selected rheumatic diseases JF - Frontiers in Medicine N2 - Background and objective: Accurate and fast diagnosis of rheumatic diseases affecting the hands is essential for further treatment decisions. Fluorescence optical imaging (FOI) visualizes inflammation-induced impaired microcirculation by increasing signal intensity, resulting in different image features. This analysis aimed to find specific image features in FOI that might be important for accurately diagnosing different rheumatic diseases. Patients and methods: FOI images of the hands of patients with different types of rheumatic diseases, such as rheumatoid arthritis (RA), osteoarthritis (OA), and connective tissue diseases (CTD), were assessed in a reading of 20 different image features in three phases of the contrast agent dynamics, yielding 60 different features for each patient. The readings were analyzed for mutual differential diagnosis of the three diseases (One-vs-One) and each disease in all data (One-vs-Rest). In the first step, statistical tools and machine-learning-based methods were applied to reveal the importance rankings of the features, that is, to find features that contribute most to the model-based classification. In the second step machine learning with a stepwise increasing number of features was applied, sequentially adding at each step the most crucial remaining feature to extract a minimized subset that yields the highest diagnostic accuracy. Results: In total, n = 605 FOI of both hands were analyzed (n = 235 with RA, n = 229 with OA, and n = 141 with CTD). All classification problems showed maximum accuracy with a reduced set of image features. For RA-vs.-OA, five features were needed for high accuracy. For RA-vs.-CTD ten, OA-vs.-CTD sixteen, RA-vs.-Rest five, OA-vs.-Rest eleven, and CTD-vs-Rest fifteen, features were needed, respectively. For all problems, the final importance ranking of the features with respect to the contrast agent dynamics was determined. Conclusions: With the presented investigations, the set of features in FOI examinations relevant to the differential diagnosis of the selected rheumatic diseases could be remarkably reduced, providing helpful information for the physician. KW - rheumatoid arthritis KW - osteoarthritis KW - connective tissue disease KW - gradient boosting machine KW - forward selection algorithm Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-17922 SN - 2296-858X VL - 10 PB - Frontiers ER - TY - JOUR A1 - Gossing, Wilhelm A1 - Frohme, Marcus A1 - Radke, Lars T1 - Biomarkers for Liquid Biopsies of Pituitary Neuroendocrine Tumors JF - Biomedicines N2 - Pituitary neuroendocrine tumors (PitNET) do not only belong to the most common intracranial neoplasms but seem to be generally more common than has been thought. Minimally invasive liquid biopsies have the potential to improve their early screening efficiency as well as monitor prognosis by facilitating the diagnostic procedures. This review aims to assess the potential of using liquid biopsies of different kinds of biomarker species that have only been obtained from solid pituitary tissues so far. Numerous molecules have been associated with the development of a PitNET, suggesting that it often develops from the cumulative effects of many smaller genetic or epigenetic changes. These minor changes eventually pile up to switch critical molecules into tumor-promoting states, which may be the key regulatory nodes representing the most potent marker substances for a diagnostic test. Drugs targeting these nodes may be superior for the therapeutic outcome and therefore the identification of such pituitary-specific cellular key nodes will help to accelerate their application in medicine. The ongoing genetic degeneration in pituitary adenomas suggests that repeated tumor profiling via liquid biopsies will be necessary for personalized and effective treatment solutions. KW - pituitary KW - biomarker KW - liquid biopsy KW - cfRNA KW - ctDNA KW - miRNA KW - epigenetics KW - lncRNA Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13307 SN - 2227-9059 VL - 8 IS - 6 ER - TY - JOUR A1 - Gossing, Wilhelm A1 - Radke, Lars A1 - Biering, Henrik A1 - Diederich, Sven A1 - Mai, Knut A1 - Frohme, Marcus T1 - The ElonginB/C-Cullin5-SOCS-Box-Complex Is a Potential Biomarker for Growth Hormone Disorders JF - Biomedicines N2 - Insulin-like growth factor 1 (IGF-1) is the standard biochemical marker for the diagnosis and treatment control of acromegaly and growth hormone deficiency (GHD). However, its limitations necessitate the screening for new specific and sensitive biomarkers. The elonginB/C-cullin5-SOCS-box-complex (ECS-complex) (an intracellular five-protein complex) is stimulated by circulating growth hormone (GH) and regulates GH receptor levels through a negative feedback loop. It mediates the cells’ sensitivity for GH and therefore, represents a potent new biomarker for those diseases. In this study, individual ECS-complex proteins were measured in whole blood samples of patients with acromegaly (n = 32) or GHD (n = 12) via ELISA and compared to controls. Hierarchical clustering of the results revealed that by combining the three ECS-complex proteins suppressor of cytokine signaling 2 (SOCS2), cullin-5 and ring-box protein 2 (Rbx-2), 93% of patient samples could be separated from controls, despite many patients having a normal IGF-1 or not receiving medical treatment. SOCS2 showed the best individual diagnostic performance with an overall accuracy of 0.93, while the combination of the three proteins correctly identified all patients and controls. This resulted in perfect sensitivity and specificity for all patient groups, which demonstrates potential benefits of the ECS-complex proteins as clinical biomarkers for the diagnostics of GH-related diseases and substantiates their important role in GH metabolism. KW - acromegaly KW - growth hormone deficiency KW - biomarker KW - SOCS2 KW - liquid biopsy KW - pituitary Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13802 SN - 2227-9059 VL - 9 IS - 2 PB - MDPI ER - TY - JOUR A1 - Marehn, David Thomas A1 - Wilhelm, Detlef A1 - Pospisil, Heike A1 - Pizzoferrato, Roberto T1 - Double entry method for the verification of data a chromatography data system receives JF - Journal of Sensors and Sensor Systems N2 - The importance of software validation increases since the need for high usability and suitability of software applications grows. In order to reduce costs and manage risk factors, more and more recommendations and rules have been established. In the field of pharmacy the vendors of so-called chromatography data systems (CDSs) had to implement the guidelines of the Code of Federal Regulations Title 21 (CFR 21) during the last few years in order to fulfill the increasing requirements. The CFR 21 part 11 deals with electronic records and signatures. This part is binding for each company in the regulated environment that wishes to create, edit and sign electronic information instead of printing them on paper. Subsection CFR 21 part 11.10(h) explains how to perform an input check for manual user entries as well as for data that will be collected from an external device. In this article we present an approach performing the double entry method on data provided by the hardware instrument in order to investigate possible influences on the raw data by the handling CDS. A software tool has been written which allows us to communicate with a high-performance liquid chromatography (HPLC) detector and acquire data from it. The communication is completely independent of a CDS which is started separately and connected to the same system. Using this configuration we made a parallel data acquisition of two instances at the same time possible. Two CDSs have been tested and for at least one of them it has been shown that a comparison of the acquired data can be done as with the double entry method for the data verification. For the second CDS we checked whether it would be applicable after a few modifications. The given approach could be either used for a live data verification of produced raw data or as a single test during a software operational qualification to verify the data acquisition functionality of the software. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12349 SN - 2194-878X VL - 8 SP - 207 EP - 214 ER - TY - JOUR A1 - Tannenberg, Robert A1 - Tscheuschner, Georg A1 - Raab, Christopher A1 - Flemig, Sabine A1 - Döring, Sarah A1 - Ponader, Marco A1 - Thurmann, Melinda A1 - Paul, Martin A1 - Weller, Michael G. T1 - Simplified Sample Preparation and Lateral Flow Immunoassay for the Detection of Plant Viruses JF - Biosensors N2 - Lateral flow immunoassays (LFAs) are widely used for on-site testing; however, their use for the rapid detection of plant viruses in the field is often limited by inconvenient sample preparation. Here, we present a new sampling method and a simplified dipstick LFA format for the detection and monitoring of cowpea chlorotic mottle virus (CCMV) as a model plant pathogen. The assay employs a monoclonal mouse antibody for capture and a poly-clonal rabbit antibody conjugated to 80 nm gold nanoparticles for detection. Conventional sample and conjugate pads are omitted, allowing the test strips to be dipped directly into wells containing plant extract and antibody–gold conjugate. No plastic casing was required, which could lead to a reduction in waste. It was shown that CCMV concentrations as low as 3.5 μg/L or 350 pg per sample could be reliably detected in 15 min. Specificity tests confirmed that other plant viruses, cowpea mosaic virus (CPMV) and tobacco mosaic virus (TMV), did not produce false-positive results. In addition, we describe a new method for on-site sampling using a manual punch and a syringe equipped with a frit. This step combines grinding the sample, extraction, filtration, and reconstitution and mixing of the antibody-gold conjugate, enabling the analysis of punched leaf disks without laboratory equipment. When applied to CCMV-infected cowpea plants, the assay revealed systemic infection before visual symptoms became apparent. This work demonstrates that simplified LFAs combined with innovative sampling techniques can provide sensitive, specific, and rapid diagnostics for crop monitoring and support early intervention strategies in agriculture. KW - LFA format KW - Vigna unguiculata KW - cowpea chlorotic mottle virus KW - crop monitoring KW - lateral flow immunoassay KW - plant virus KW - sample preparation KW - waste reduction Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21294 VL - 16 IS - 2 PB - MDPI ER - TY - JOUR A1 - Zesch, Christoph A1 - Finkelstein, Daniel A1 - Regehly, Martin A1 - Reif, Jürgen A1 - Schrader, Sigurd T1 - Efficient nanosecond barium nitrate Raman laser for the visible spectral range JF - Optics Express N2 - We report on the design and performance of two solid-state Raman lasers, pumped by a frequency-doubled Nd:YAG laser at 532 nm (8 ns FWHM, 10 Hz, up to 10 mJ), that generate either the first or second Stokes radiation with wavelengths of 563 nm and 598 nm. Barium nitrate crystals with a 1 cm² face area and lengths of 3 cm and 5 cm were used as the Raman-active medium in stable semi-spherical resonators. The Raman lasers produced first Stokes and second Stokes pulses, achieving pulse energies up to 2.3 mJ and conversion efficiencies up to 35% (first Stokes, 5 cm crystal) and 21% (second Stokes, 5 cm crystal). The output beams exhibit nearly Gaussian profiles with beam quality factors of M² = 1.67 and M² = 1.83, respectively. The proposed design and characterization demonstrated a Raman laser with high efficiency and superior beam quality. After subsequent frequency doubling, the output appears well dedicated for future differential absorption LiDAR (DIAL) applications. KW - Gaussian beam KW - laser source KW - Nd:YAG laser KW - Raman laser KW - solid state laser KW - ultraviolet laser Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21320 VL - 34 IS - 4 SP - 6355 EP - 6363 PB - Optica ER - TY - JOUR A1 - Steglich, Patrick A1 - Mai, Christian A1 - Villringer, Claus A1 - Dietzel, Birgit A1 - Bondarenko, Siegfried A1 - Ksianzou, Viachaslau A1 - Villasmunta, Francesco A1 - Zesch, Christoph A1 - Pulwer, Silvio A1 - Burger, Martin A1 - Bauer, Joachim A1 - Heinrich, Friedhelm A1 - Schrader, Sigurd A1 - Vitale, Francesco A1 - De Matteis, Fabio A1 - Prosposito, Paolo A1 - Casalboni, Mauro A1 - Mai, Andreas T1 - Silicon-organic hybrid photonics: an overview of recent advances, electro-optical effects and CMOS integration concepts JF - Journal of Physics: Photonics N2 - In recent decades, much research effort has been invested in the development of photonic integrated circuits, and silicon-on-insulator technology has been established as a reliable platform for highly scalable silicon-based electro-optical modulators. However, the performance of such devices is restricted by the inherent material properties of silicon. An approach to overcoming these deficiencies is to integrate organic materials with exceptionally high optical nonlinearities into a silicon-on-insulator photonic platform. Silicon–organic hybrid photonics has been shown to overcome the drawbacks of silicon-based modulators in terms of operating speed, bandwidth, and energy consumption. This work reviews recent advances in silicon–organic hybrid photonics and covers the latest improvements to single components and device concepts. Special emphasis is given to the in-device performance of novel electro-optical polymers and the use of different electro-optical effects, such as the linear and quadratic electro-optical effect, as well as the electric-field-induced linear electro-optical effect. Finally, the inherent challenges of implementing non-linear optical polymers on a silicon photonic platform are discussed and a perspective for future directions is given. Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13882 SN - 2515-7647 VL - 3 IS - 2 ER - TY - JOUR A1 - Regehly, Martin A1 - Hecht, Stefan T1 - Split-Aperture Xolography – Linear Volumetric Photoactivation with Short Axial Dimension and Low out of Focus Excitation JF - Advanced Science N2 - Spatially confined photo-excitation with the lowest possible activation of the remaining volume is of central importance for high-resolution high-density optical data storage, fluorescence microscopy, 3D-lithography, and 3D-printing. Two-photon absorption (2PA) enables such applications yet leads to slow processing speed due to the underlying non-linear absorption process. Here, Split-Aperture Xolography (SAX), is introduced which uses stepwise excitation of dual-color responsive molecules to initiate a linear volumetric photo-reaction process that is up to several orders of magnitude more efficient than 2PA. The capabilities of SAX are investigated in a scenario study for focusing systems with high numerical aperture (NA) using a Python implementation of vectorial diffraction theory. The intersecting half-cones generated by the split illuminated entrance aperture of the objective reduce the axial focal spot size of the activation distribution by up to a factor of two compared to 2PA targeting the same electronic transition. A steep average decline of the activation probability with the fifth power away from focus is found for a wide range of directions. This is significantly better in comparison to 2PA and prevents that undesired out-of-focus excitation events sum-up with subsequent irradiations. This approach is expected to be advantageous for volumetric methods at the nanoscale. KW - photoswitch KW - split-aperture xolography KW - two-photon absorption KW - vectorial diffraction KW - volumetric 3D printing Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20749 VL - 12 IS - 31 PB - Wiley ER - TY - JOUR A1 - Bauer, Joachim A1 - Heinrich, Friedhelm A1 - Villasmunta, Francesco A1 - Villringer, Claus A1 - Reck, Johanna A1 - Peters, Sven A1 - Treffer, Alexander A1 - Kuhnt, Christian A1 - Marschmeyer, Steffen A1 - Fursenko, Oksana A1 - Stolarek, David A1 - Mai, Andreas A1 - Regehly, Martin T1 - Reflectometric method for measuring residual oxides in through-silicon vias for 3D chip integration JF - Optics Express N2 - A significant aspect of fabricating 3D chip architectures is ensuring proper contact between the different layers of the chip, which often requires removing the underside of isolation layers before filling vias with conductive material. Currently, scanning electron microscopy is the established method for investigating such structures. In this paper, we propose a rapid, non-destructive optical analysis technique for the simultaneous measurement of through-silicon vias (TSV) depths, silicon wafer thickness, and residual oxide thickness. The proposed method utilizes Fourier peak shift analysis (FPSA) of reflectance measurements in the near-infrared (1200 nm—2200 nm) spectral regions. The application of FPSA to representative samples taken from a commercial TSV integration process for MEMS and CMOS fabrication demonstrated good agreement with reference scanning electron microscopy measurements, confirming the feasibility of the method for in-line and in-situ metrology. The results indicate that FPSA has great potential for real-time process monitoring and control during 3D chip manufacturing. KW - effective refractive index KW - Fourier transforms KW - near infrared KW - ray tracing KW - scanning electron microscopy KW - thin film Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20633 VL - 33 IS - 15 SP - 32175 EP - 32189 PB - Optica ER - TY - JOUR A1 - Holland-Moritz, Ralph A1 - Vandenhouten, Ralf T1 - Detection of Movement Profiles in Facility Management Using Complex Event Processing JF - International Journal of Information Science N2 - This paper presents a solution for detecting movement and spreading profiles by analyzing sensor data in the facility management. First of all, a scenario is defined and described. On the basis of this scenario an approach is made using integration of events over time and location. For making the integration possible the required information, which the data objects have to provide, is identified. At the end a solution is presented using Complex Event Processing to implement the integration of events. KW - facility management KW - complex event processing KW - sensor KW - detector KW - movement profile KW - spatial dimension KW - integration KW - alarm reliability KW - event driven architecture KW - evaluation KW - security KW - intrusion KW - fire Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9495 UR - http://article.sapub.org/10.5923.j.ijis.20130301.02.html SN - 2163-193X VL - 3 IS - 1 SP - 7 EP - 12 ER - TY - JOUR A1 - Balabanov, Stefan A1 - Wilhelm, Thomas A1 - Venz, Simone A1 - Keller, Gunhild A1 - Scharf, Christian A1 - Pospisil, Heike A1 - Braig, Melanie A1 - Barett, Christine A1 - Bokemeyer, Carsten A1 - Walther, Reinhard A1 - Brümmendorf, Tim H. A1 - Schuppert, Andreas T1 - Combination of a Proteomics Approach and Reengineering of Meso Scale Network Models for Prediction of Mode-of-Action for Tyrosine Kinase Inhibitors JF - PLoS ONE N2 - In drug discovery, the characterisation of the precise modes of action (MoA) and of unwanted off-target effects of novel molecularly targeted compounds is of highest relevance. Recent approaches for identification of MoA have employed various techniques for modeling of well defined signaling pathways including structural information, changes in phenotypic behavior of cells and gene expression patterns after drug treatment. However, efficient approaches focusing on proteome wide data for the identification of MoA including interference with mutations are underrepresented. As mutations are key drivers of drug resistance in molecularly targeted tumor therapies, efficient analysis and modeling of downstream effects of mutations on drug MoA is a key to efficient development of improved targeted anti-cancer drugs. Here we present a combination of a global proteome analysis, reengineering of network models and integration of apoptosis data used to infer the mode-of-action of various tyrosine kinase inhibitors (TKIs) in chronic myeloid leukemia (CML) cell lines expressing wild type as well as TKI resistance conferring mutants of BCR-ABL. The inferred network models provide a tool to predict the main MoA of drugs as well as to grouping of drugs with known similar kinase inhibitory activity patterns in comparison to drugs with an additional MoA. We believe that our direct network reconstruction approach, demonstrated on proteomics data, can provide a complementary method to the established network reconstruction approaches for the preclinical modeling of the MoA of various types of targeted drugs in cancer treatment. Hence it may contribute to the more precise prediction of clinically relevant on- and off-target effects of TKIs. Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6370 SN - 1932-6203 VL - 8 IS - 1 ER - TY - JOUR A1 - Thiemann, Alexander A1 - Fu, Junjie A1 - Seifert, Felix A1 - Grant-Downton, Robert T A1 - Schrag, Tobias A A1 - Pospisil, Heike A1 - Frisch, Matthias A1 - Melchinger, Albrecht E A1 - Scholten, Stefan T1 - Genome-wide meta-analysis of maize heterosis reveals the potential role of additive gene expression at pericentromeric loci JF - BMC Plant Biology N2 - The identification of QTL involved in heterosis formation is one approach to unravel the not yet fully understood genetic basis of heterosis - the improved agronomic performance of hybrid F1 plants compared to their inbred parents. The identification of candidate genes underlying a QTL is important both for developing markers and determining the molecular genetic basis of a trait, but remains difficult owing to the large number of genes often contained within individual QTL. To address this problem in heterosis analysis, we applied a meta-analysis strategy for grain yield (GY) of Zea mays L. as example, incorporating QTL-, hybrid field-, and parental gene expression data. KW - heterosis KW - maize KW - QTL KW - grain yield KW - additive gene expression Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5999 SN - 1471-2229 VL - 14 IS - 88 ER - TY - JOUR A1 - Ummanni, Ramesh A1 - Mundt, Frederike A1 - Pospisil, Heike A1 - Venz, Simone A1 - Scharf, Christian A1 - Barett, Christine A1 - Fälth, Maria A1 - Köllermann, Jens A1 - Walther, Reinhard A1 - Schlomm, Thorsten A1 - Sauter, Guido A1 - Bokemeyer, Carsten A1 - Sültmann, Holger A1 - Schuppert, Andreas A1 - Brümmendorf, Tim H. A1 - Balabanov, Stefan T1 - Identification of Clinically Relevant Protein Targets in Prostate Cancer with 2D-DIGE Coupled Mass Spectrometry and Systems Biology Network Platform JF - PLoS ONE N2 - Prostate cancer (PCa) is the most common type of cancer found in men and among the leading causes of cancer death in the western world. In the present study, we compared the individual protein expression patterns from histologically characterized PCa and the surrounding benign tissue obtained by manual micro dissection using highly sensitive two-dimensional differential gel electrophoresis (2D-DIGE) coupled with mass spectrometry. Proteomic data revealed 118 protein spots to be differentially expressed in cancer (n = 24) compared to benign (n = 21) prostate tissue. These spots were analysed by MALDI-TOF-MS/MS and 79 different proteins were identified. Using principal component analysis we could clearly separate tumor and normal tissue and two distinct tumor groups based on the protein expression pattern. By using a systems biology approach, we could map many of these proteins both into major pathways involved in PCa progression as well as into a group of potential diagnostic and/or prognostic markers. Due to complexity of the highly interconnected shortest pathway network, the functional sub networks revealed some of the potential candidate biomarker proteins for further validation. By using a systems biology approach, our study revealed novel proteins and molecular networks with altered expression in PCa. Further functional validation of individual proteins is ongoing and might provide new insights in PCa progression potentially leading to the design of novel diagnostic and therapeutic strategies. Y1 - 2011 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6432 SN - 1932-6203 VL - 6 IS - 2 ER - TY - JOUR A1 - Standfuß, Christoph A1 - Pospisil, Heike A1 - Klein, Andreas T1 - SNP microarray analyses reveal copy number alterations and progressive genome reorganization during tumor development in SVT/t driven mice breast cancer JF - BMC Cancer N2 - Tumor development is known to be a stepwise process involving dynamic changes that affect cellular integrity and cellular behavior. This complex interaction between genomic organization and gene, as well as protein expression is not yet fully understood. Tumor characterization by gene expression analyses is not sufficient, since expression levels are only available as a snapshot of the cell status. So far, research has mainly focused on gene expression profiling or alterations in oncogenes, even though DNA microarray platforms would allow for high-throughput analyses of copy number alterations (CNAs). KW - breast cancer KW - genome reorganization KW - copy number alteration KW - CNV KW - fragile site Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6414 SN - 1471-2407 VL - 12 IS - 380 ER - TY - JOUR A1 - Klein, Andreas A1 - Pospisil, Heike T1 - Gene Expression Profiling of Pancreatic Cancer Reveals a Significant Deregulation of the TGF-β Pathway and the Discovery of Genes for Prognosis JF - Global Journal of Human Genetics & Gene Therapy N2 - We have re-analyzed previously published gene expression data from ninety-four pancreatic ductal adenocarcinomas (PDAC) samples. We determined the gene expression profile of genes differentially expressed in PDAC compared to non-malignant pancreatic tissue. Using the 100 top-ranked genes, we were able to discriminate between PDAC and non-malignant pancreatic tissue. A hierarchical cluster analysis revealed only a 6 % false discovery rate. The prognostic strength of these discriminative genes was underscored by a SVM classification and 3-fold cross validation with an 89 % correct class assignment. The annotation of the 100 top-ranked genes revealed that most of the genes were involved in the processes of signal transduction, cell adhesion, extracellular matrix organization and cell migration. The most greatly affected signal cascade was the transforming growth factor β receptor signaling pathway, which was significantly enriched in the top-ranked genes. Furthermore, we identified eleven genes that were associated with good prognosis. KW - pancreatic ductal adenocarcinoma KW - gene expression profiling KW - TGF-β Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6519 SN - 2311-0309 VL - 2 IS - 1 SP - 30 EP - 39 ER - TY - JOUR A1 - Hannemann, Juliane A1 - Meyer-Staeckling, Sönke A1 - Kemming, Dirk A1 - Alpers, Iris A1 - Joosse, Simon A. A1 - Pospisil, Heike A1 - Kurtz, Stefan A1 - Görndt, Jennifer A1 - Püschel, Klaus A1 - Riethdorf, Sabine A1 - Pantel, Klaus A1 - Brandt, Burkhard T1 - Quantitative High-Resolution Genomic Analysis of Single Cancer Cells JF - PLoS ONE N2 - During cancer progression, specific genomic aberrations arise that can determine the scope of the disease and can be used as predictive or prognostic markers. The detection of specific gene amplifications or deletions in single blood-borne or disseminated tumour cells that may give rise to the development of metastases is of great clinical interest but technically challenging. In this study, we present a method for quantitative high-resolution genomic analysis of single cells. Cells were isolated under permanent microscopic control followed by high-fidelity whole genome amplification and subsequent analyses by fine tiling array-CGH and qPCR. The assay was applied to single breast cancer cells to analyze the chromosomal region centred by the therapeutical relevant EGFR gene. This method allows precise quantitative analysis of copy number variations in single cell diagnostics. Y1 - 2011 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6426 SN - 1932-6203 VL - 6 IS - 11 ER - TY - JOUR A1 - Silbermann, Jascha A1 - Wernicke, Catrin A1 - Pospisil, Heike A1 - Frohme, Marcus T1 - RefPrimeCouch—a reference gene primer CouchApp JF - Database: The Journal of Biological Databases and Curation N2 - To support a quantitative real-time polymerase chain reaction standardization project, a new reference gene database application was required. The new database application was built with the explicit goal of simplifying not only the development process but also making the user interface more responsive and intuitive. To this end, CouchDB was used as the backend with a lightweight dynamic user interface implemented client-side as a one-page web application. Data entry and curation processes were streamlined using an OpenRefine-based workflow. The new RefPrimeCouch database application provides its data online under an Open Database License. Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6330 SN - 1758-0463 VL - 2013 ER - TY - JOUR A1 - Duscharla, Divya A1 - Bhumireddy, Sudarshana Reddy A1 - Lakshetti, Sridhar A1 - Pospisil, Heike A1 - Murthy, P. V. L. N. A1 - Walther, Reinhard A1 - Sripadi, Prabhakar A1 - Ummanni, Ramesh T1 - Prostate Cancer Associated Lipid Signatures in Serum Studied by ESI-Tandem Mass Spectrometryas Potential New Biomarkers JF - PLoS ONE N2 - Prostate cancer (PCa) is one amongst the most common cancersin western men. Incidence rate ofPCa is on the rise worldwide. The present study deals with theserum lipidome profiling of patients diagnosed with PCa to identify potential new biomarkers. We employed ESI-MS/MS and GC-MS for identification of significantly altered lipids in cancer patient’s serum compared to controls. Lipidomic data revealed 24 lipids are significantly altered in cancer patinet’s serum (n = 18) compared to normal (n = 18) with no history of PCa. By using hierarchical clustering and principal component analysis (PCA) we could clearly separate cancer patients from control group. Correlation and partition analysis along with Formal Concept Analysis (FCA) have identified that PC (39:6) and FA (22:3) could classify samples with higher certainty. Both the lipids, PC (39:6) and FA (22:3) could influence the cataloging of patients with 100% sensitivity (all 18 control samples are classified correctly) and 77.7% specificity (of 18 tumor samples 4 samples are misclassified) with p-value of 1.612×10−6 in Fischer’s exact test. Further, we performed GC-MS to denote fatty acids altered in PCa patients and found that alpha-linolenic acid (ALA) levels are altered in PCa. We also performed an in vitro proliferation assay to determine the effect of ALA in survival of classical human PCa cell lines LNCaP and PC3. We hereby report that the altered lipids PC (39:6) and FA (22:3) offer a new set of biomarkers in addition to the existing diagnostic tests that could significantly improve sensitivity and specificity in PCa diagnosis. Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5863 SN - 1932-6203 VL - 11 IS - 3 ER - TY - JOUR A1 - Gladisch, Johannes A1 - Sarauli, David A1 - Schäfer, Daniel A1 - Dietzel, Birgit A1 - Schulz, Burkhard A1 - Lisdat, Fred T1 - Towards a novel bioelectrocatalytic platform based on “wiring” of pyrroloquinoline quinone-dependent glucose dehydrogenase with an electrospun conductive polymeric fiber architecture JF - Scientific Reports N2 - Electrospinning is known as a fabrication technique for electrode architectures that serve as immobilization matrices for biomolecules. The current work demonstrates a novel approach to construct a conductive polymeric platform, capable not only of immobilization, but also of electrical connection of the biomolecule with the electrode. It is produced upon electrospinning from mixtures of three different highly conductive sulfonated polyanilines and polyacrylonitrile on ITO electrodes. The resulting fiber mats are with a well-retained conductivity. After coupling the enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) to polymeric structures and addition of the substrate glucose an efficient bioelectrocatalysis is demonstrated. Depending on the choice of the sulfonated polyanilline mediatorless bioelectrocatalysis starts at low potentials; no large overpotential is needed to drive the reaction. Thus, the electrospun conductive immobilization matrix acts here as a transducing element, representing a promising strategy to use 3D polymeric scaffolds as wiring agents for active enzymes. In addition, the mild and well reproducible fabrication process and the active role of the polymer film in withdrawing electrons from the reduced PQQ-GDH lead to a system with high stability. This could provide access to a larger group of enzymes for bioelectrochemical applications including biosensors and biofuel cells. Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5846 SN - 2045-2322 VL - 6 ER - TY - JOUR A1 - Seifert, Felix A1 - Thiemann, Alexander A1 - Pospisil, Heike A1 - Scholten, Stefan T1 - Re-annotation of the maize oligonucleotide array JF - Maydica N2 - The microarray technology has become an established approach for large-scale gene expression analysis with mature protocols for sample, microarray, and data processing. The maize oligonucleotide array (maizearray) is one of the few microarray platforms designed for genome-wide gene expression analysis in Zea mays L. Many datas-ets addressing various genetic, physiological and developmental topics generated with this platform are available. The original 57,452 microarray probes were compiled based on expressed sequence tags (ESTs). Meanwhile the maize genome sequence became available providing the possibility for an improved annotation of the microar-ray probe set. In this study we determined the genome positions of all maizearray probes to obtain current gene annotations and generated current Gene Ontology (GO) annotations. These new data allow tracing redundancy of the probe set and interfering cross-hybridizations, and doubled the number of genes with functional GO data. Our re-annotation will largely improve the functional analysis of available and future datasets generated on this microarray platform. KW - maize oligonucleotide array KW - microarray KW - annotation Y1 - 2012 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6178 UR - https://journals-crea.4science.it/index.php/maydica/article/view/628 SN - 0025-6153 VL - 57 IS - 1 SP - 49 EP - 55 ER - TY - JOUR A1 - Marczok, Stefanie A1 - Bortz, Birgit A1 - Wang, Chong A1 - Pospisil, Heike T1 - Comprehensive Analysis of Genome Rearrangements in Eight Human Malignant Tumor Tissues JF - PLoS ONE N2 - Carcinogenesis is a complex multifactorial, multistage process, but the precise mechanisms are not well understood. In this study, we performed a genome-wide analysis of the copy number variation (CNV), breakpoint region (BPR) and fragile sites in 2,737 tumor samples from eight tumor entities and in 432 normal samples. CNV detection and BPR identification revealed that BPRs tended to accumulate in specific genomic regions in tumor samples whereas being dispersed genome-wide in the normal samples. Hotspots were observed, at which segments with similar alteration in copy number were overlapped along with BPRs adjacently clustered. Evaluation of BPR occurrence frequency showed that at least one was detected in about and more than 15% of samples for each tumor entity while BPRs were maximal in 12% of the normal samples. 127 of 2,716 tumor-relevant BPRs (termed ‘common BPRs’) exhibited also a noticeable occurrence frequency in the normal samples. Colocalization assessment identified 20,077 CNV-affecting genes and 169 of these being known tumor-related genes. The most noteworthy genes are KIAA0513 important for immunologic, synaptic and apoptotic signal pathways, intergenic non-coding RNA RP11-115C21.2 possibly acting as oncogene or tumor suppressor by changing the structure of chromatin, and ADAM32 likely importance in cancer cell proliferation and progression by ectodomain-shedding of diverse growth factors, and the well-known tumor suppressor gene p53. The BPR distributions indicate that CNV mutations are likely non-random in tumor genomes. The marked recurrence of BPRs at specific regions supports common progression mechanisms in tumors. The presence of hotspots together with common BPRs, despite its small group size, imply a relation between fragile sites and cancer-gene alteration. Our data further suggest that both protein-coding and non-coding genes possessing a range of biological functions might play a causative or functional role in tumor biology. This research enhances our understanding of the mechanisms for tumorigenesis and progression. Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6607 SN - 1932-6203 VL - 11 IS - 7 ER - TY - JOUR A1 - Pfeil, Juliane A1 - Siptroth, Julienne A1 - Pospisil, Heike A1 - Frohme, Marcus A1 - Hufert, Frank T. A1 - Moskalenko, Olga A1 - Yateem, Murad A1 - Nechyporenko, Alina T1 - Classification of Microbiome Data from Type 2 Diabetes Mellitus Individuals with Deep Learning Image Recognition JF - Big Data and Cognitive Computing N2 - Microbiomic analysis of human gut samples is a beneficial tool to examine the general well-being and various health conditions. The balance of the intestinal flora is important to prevent chronic gut infections and adiposity, as well as pathological alterations connected to various diseases. The evaluation of microbiome data based on next-generation sequencing (NGS) is complex and their interpretation is often challenging and can be ambiguous. Therefore, we developed an innovative approach for the examination and classification of microbiomic data into healthy and diseased by visualizing the data as a radial heatmap in order to apply deep learning (DL) image classification. The differentiation between 674 healthy and 272 type 2 diabetes mellitus (T2D) samples was chosen as a proof of concept. The residual network with 50 layers (ResNet-50) image classification model was trained and optimized, providing discrimination with 96% accuracy. Samples from healthy persons were detected with a specificity of 97% and those from T2D individuals with a sensitivity of 92%. Image classification using DL of NGS microbiome data enables precise discrimination between healthy and diabetic individuals. In the future, this tool could enable classification of different diseases and imbalances of the gut microbiome and their causative genera. KW - human intestinal microbiome KW - next-generation sequencing KW - type 2 diabetes KW - deep learning KW - image classification Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-17184 SN - 2504-2289 VL - 7 IS - 1 PB - MDPI ER - TY - JOUR A1 - Siptroth, Julienne A1 - Moskalenko, Olga A1 - Krumbiegel, Carsten A1 - Ackermann, Jörg A1 - Koch, Ina A1 - Pospisil, Heike T1 - Variation of butyrate production in the gut microbiome in type 2 diabetes patients JF - International Microbiology N2 - Background Diabetes mellitus type 2 is a common disease that poses a challenge to the healthcare system. The disease is very often diagnosed late. A better understanding of the relationship between the gut microbiome and type 2 diabetes can support early detection and form an approach for therapies. Microbiome analysis offers a potential opportunity to find markers for this disease. Next-generation sequencing methods can be used to identify the bacteria present in the stool sample and to generate a microbiome profile through an analysis pipeline. Statistical analysis, e.g., using Student’s t-test, allows the identification of significant differences. The investigations are not only focused on single bacteria, but on the determination of a comprehensive profile. Also, the consideration of the functional microbiome is included in the analyses. The dataset is not from a clinical survey, but very extensive. Results By examining 946 microbiome profiles of diabetes mellitus type 2 sufferers (272) and healthy control persons (674), a large number of significant genera (25) are revealed. It is possible to identify a large profile for type 2 diabetes disease. Furthermore, it is shown that the diversity of bacteria per taxonomic level in the group of persons with diabetes mellitus type 2 is significantly reduced compared to a healthy control group. In addition, six pathways are determined to be significant for type 2 diabetes describing the fermentation to butyrate. These parameters tend to have high potential for disease detection. Conclusions With this investigation of the gut microbiome of persons with diabetes type 2 disease, we present significant bacteria and pathways characteristic of this disease. KW - NGS KW - type 2 diabetes KW - gut KW - butyrate KW - statistical analysis Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-17802 VL - 26 SP - 601 EP - 610 PB - Springer Nature ER - TY - JOUR A1 - Siptroth, Julienne A1 - Moskalenko, Olga A1 - Krumbiegel, Carsten A1 - Ackermann, Jörg A1 - Koch, Ina A1 - Pospisil, Heike T1 - Investigation of metabolic pathways from gut microbiome analyses regarding type 2 diabetes mellitus using artificial neural networks JF - Discover Artificial Intelligence N2 - Background Type 2 diabetes mellitus is a prevalent disease that contributes to the development of various health issues, including kidney failure and strokes. As a result, it poses a significant challenge to the worldwide healthcare system. Research into the gut microbiome has enabled the identification and description of various diseases, with bacterial pathways playing a critical role in this context. These pathways link individual bacteria based on their biological functions. This study deals with the classification of microbiome pathway profiles of type 2 diabetes mellitus patients. Methods Pathway profiles were determined by next-generation sequencing of 16S rDNA from stool samples, which were subsequently assigned to bacteria. Then, the involved pathways were assigned by the identified gene families. The classification of type 2 diabetes mellitus is enabled by a constructed neural network. Furthermore, a feature importance analysis was performed via a game theoretic approach (SHapley Additive exPlanations). The study not only focuses on the classification using neural networks, but also on identifying crucial bacterial pathways. Results It could be shown that a neural network classification of type 2 diabetes mellitus and a healthy comparison group is possible with an excellent prediction accuracy. It was possible to create a ranking to identify the pathways that have a high impact on the model prediction accuracy. In this way, new associations between the alteration of, e.g. a biosynthetic pathway and the presence of diabetes mellitus type 2 disease can also be discovered. The basis is formed by 946 microbiome pathway profiles from diabetes mellitus type 2 patients (272) and healthy comparison persons (674). Conclusion With this study of the gut microbiome, we present an approach using a neural network to obtain a classification of healthy and type 2 diabetes mellitus and to identify the critical features. Intestinal bacteria pathway profiles form the basis. KW - type 2 diabetes mellitus KW - gut KW - NGS KW - artificial neural network KW - machine learning KW - explainable artificial intelligence (XAI) Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-17497 SN - 2731-0809 VL - 3 IS - 1 PB - Springer Nature ER - TY - BOOK ED - Assmann, Tom ED - Bürklen, Anna ED - Gruber, Johannes ED - Knese, Dennis ED - Mayregger, Patrick ED - Rudolph, Christian T1 - Radlogistik: Grundlagen zu Logistik und Wirtschaftsverkehr mit Lasten- und Transporträdern N2 - Aus den Straßen europäischer Städte sind sie kaum noch wegzudenken: Lasten- und Transporträder. Im Personenverkehr sind sie für Einkäufe und Kindertransporte bereits Standard, ebenso im Kuriersegment sowie der Paketzustellung. Besonders in der Logistik und im Wirtschaftsverkehr liegt noch ein sehr breit gefächertes und ausdifferenziertes Einsatzpotenzial. Die Renaissance des Lastenrads als Transportmittel nimmt auch im gewerblichen Sektor an Bedeutung zu. Das Open Access-Buch bietet in dieser Form erstmals systematisch aufbereitetes Wissen für Praktiker in Wirtschaft, Politik und Verwaltung sowie für Studierende in den Bereichen Logistik und Verkehrsplanung. Zu den einzelnen Aspekten der Radlogistik (u. a. Fahrzeuge und Technik, gewerblicher Einsatz, logistischer Einsatz, Planung, Infrastrukturen, intermodale Einbindung, Best-Practice-Beispiele, Entwicklungstrends) haben national renommierte Wissenschaftler:innen Beiträge verfasst. Dieses Buch bündelt den aktuellen Wissensstand auf Basis von Forschungsergebnissen und stellt ihn kompakt und übersichtlich dar. KW - Güterverkehr KW - Lastenrad KW - Letzte Meile KW - Logistik KW - Logistikplanung KW - Verkehr KW - Verkehrsplanung KW - Wirtschaftsverkehr Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-19318 SN - 978-3-658-44449-5 ER - TY - JOUR A1 - Herold, Marcel A1 - Roedenbeck, Marc T1 - AI-Driven Research in the Recruitment and Selection Process: Application of an AI Taxonomy With a Systematic Literature Review JF - SAGE Open N2 - A review of the literature on the application of artificial intelligence (AI) in the recruitment and selection process (RSP) was conducted, but no relevant studies were identified. While several reviews have focussed on AI in human resource management in general, none of these have examined the RSP in detail or employed an AI taxonomy for clustering. Consequently, we applied an AI taxonomy identified in the literature with the aim to identify the stages of the RSP in the focus of research and the algorithms mostly used. We conducted a systematic literature review underpinned by a concept matrix, complemented by a computational literature review (CLR), that employed natural language processing (NLP). The initial 4,579 studies were sourced from three databases and narrowed down to a total of 502. Our major findings indicate that the majority of studies were categorised under the stages “assessment & selection” and “processing incoming applications” in the RSP. The predominant algorithms in use pertain to the field of NLP and machine learning. The CLR emphasised the significance of ethics in AI research. While our study has expanded the general AI taxonomy by incorporating an ethical perspective and is one of the studies with the most articles used to reflect this topic, it is solely focussing on describing the past. Nevertheless, this article helps to align research on exploring and testing alternative approaches with those most frequently used. KW - AI KW - HRM KW - computational literature review KW - literature review KW - recruitment and selection KW - artificial intelligence KW - human resources management (HRM) Y1 - 2025 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20736 VL - 15 IS - 3 PB - Sage ER - TY - JOUR A1 - Feifel, Sven Christian A1 - Stieger, Kai Ralf A1 - Kapp, Andreas A1 - Weber, Dennis A1 - Allegrozzi, Marco A1 - Piccioli, Mario A1 - Turano, Paola A1 - Lisdat, Fred T1 - Insights into Interprotein Electron Transfer of Human Cytochrome c Variants Arranged in Multilayer Architectures by Means of an Artificial Silica Nanoparticle Matrix JF - ACS Omega N2 - The redox behavior of proteins plays a crucial part in the design of bioelectronic systems. We have demonstrated several functional systems exploiting the electron exchange properties of the redox protein cytochrome c (cyt c) in combination with enzymes and photoactive proteins. The operation is based on an effective reaction at modified electrodes but also to a large extent on the capability of self-exchange between cyt c molecules in a surface-fixed state. In this context, different variants of human cyt c have been examined here with respect to an altered heterogeneous electron transfer (ET) rate in a monolayer on electrodes as well as an enhanced self-exchange rate while being incorporated in multilayer architectures. For this purpose, mutants of the wild-type (WT) protein have been prepared to change the chemical nature of the surface contact area near the heme edge. The structural integrity of the variants has been verified by NMR and UV–vis measurements. It is shown that the single-point mutations can significantly influence the heterogeneous ET rate at thiol-modified gold electrodes and that electroactive protein/silica nanoparticle multilayers can be constructed with all forms of human cyt c prepared. The kinetic behavior of electron exchange for the mutant proteins in comparison with that of the WT has been found altered in some multilayer arrangements. Higher self-exchange rates have been found for K79A. The results demonstrate that the position of the introduced change in the charge situation of cyt c has a profound influence on the exchange behavior. In addition, the behavior of the cyt c variants in assembled multilayers is found to be rather similar to the situation of cyt c self-exchange in solution verified by NMR. Y1 - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-6753 SN - 2470-1343 VL - 1 IS - 6 SP - 1058 EP - 1066 ER - TY - JOUR A1 - Feifel, Sven Christian A1 - Lisdat, Fred T1 - Silica nanoparticles for the layer-by-layer assembly of fully electro-active cytochrome c multilayers JF - Journal of Nanobiotechnology N2 - For bioanalytical systems sensitivity and biomolecule activity are critical issues. The immobilization of proteins into multilayer systems by the layer-by-layer deposition has become one of the favorite methods with this respect. Moreover, the combination of nanoparticles with biomolecules on electrodes is a matter of particular interest since several examples with high activities and direct electron transfer have been found. Our study describes the investigation on silica nanoparticles and the redox protein cytochrome c for the construction of electro-active multilayer architectures, and the electron transfer within such systems. The novelty of this work is the construction of such artificial architectures with a non-conducting building block. Furthermore a detailed study of the size influence of silica nanoparticles is performed with regard to formation and electrochemical behavior of these systems. Y1 - 2011 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-5819 SN - 1477-3155 VL - 9 IS - 59 ER - TY - CHAP A1 - Gruber, Johannes A1 - Plener, Martin A1 - Rudolph, Christian A1 - Seiffert, Robert ED - Assmann, Tom ED - Bürklen, Anna ED - Gruber, Johannes ED - Knese, Dennis ED - Mayregger, Patrick ED - Rudolph, Christian T1 - Gewerblicher Einsatz von Lastenrädern – Vielfalt der Nutzungsmöglichkeiten T2 - Radlogistik: Grundlagen zu Logistik und Wirtschaftsverkehr mit Lasten- und Transporträdern N2 - Dieser Beitrag beleuchtet die Vielfalt der Nutzungsmöglichkeiten von Lastenrädern im gewerblichen Einsatz, welche sich am stetig wachsenden Interesse von privatwirtschaftlichen und öffentlichen Organisationen an der Lastenradnutzung zeigt. Am Beispiel der rund 750 Teilnehmenden an einem deutschlandweiten Lastenrad-Testprogramm werden gewerbliche Nutzer charakterisiert. Des Weiteren bietet der Beitrag einen Vorschlag zur Segmentierung der äußerst heterogenen Anwendungsfelder, die sich nicht nur auf Logistik- und Dienstleistungsbranchen beschränken. Y1 - 2024 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-19737 SP - 67 EP - 88 PB - Springer Fachmedien Wiesbaden ER - TY - JOUR A1 - Adomavičiũtė-Grabusovė, Sonata A1 - Hinkel, Jonas A1 - Usenov, Iskander A1 - Novikov, Alexander S. A1 - Sakharova, Tatiana A1 - Döhler, Torsten A1 - Geißler, Ute A1 - Feliksberger, Elena A1 - Artyushenko, Viacheslav T1 - Microstructuring of the end-surface for silver halide polycrystalline fibers to suppress Fresnel reflection JF - Optical Materials Express N2 - Silver halide polycrystalline infrared fibers (PIR) have unique properties such as excellent transmittance in the spectral range from 3 to 17 µm, while also being highly flexible, non-toxic, and non-hygroscopic. They are used in industry and medicine for CO2-laser power delivery, flexible infrared imaging, and remote process spectroscopy. While PIR fibers possess a quite low attenuation (0.1-0.5 dB/m) in the 8-12 µm range, their total transmittance is limited by significant Fresnel reflections at the fiber end faces due to the high refractive index of silver halide (>2.1). Functionalization of these surfaces with specially designed Anti-Reflective Microstructures (ARMs) enables a striking enhancement of fiber transmittance. In this work, direct imprinting (or embossing) of microstructures to fiber ends and their profiling with a microstructured knife was applied to fabricate such ARMs. The resulting two-dimensional Moth-eye microstructures and one-dimensional microgrooves at the PIR-fiber ends enable to an increase of fiber transmittance in a broadband range of (5-17 µm) as well as to reach up to 20% improvement for PIR-fiber laser cables used for power delivery of CO2-lasers at 10.6 µm. KW - effective refractive index KW - fresnel reflection KW - infrared fiber KW - infrared imaging KW - quantum cascade semiconductor laser KW - refractive index Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-15549 VL - 12 IS - 1 SP - 34 EP - 48 PB - Optica Publishing Group ER - TY - JOUR A1 - Tarabrin, Mikhail K. A1 - Bushunov, Andrey A. A1 - Teslenko, Andrei A. A1 - Sakharova, Tatiana A1 - Hinkel, Jonas A1 - Usenov, Iskander A1 - Döhler, Torsten A1 - Geißler, Ute A1 - Artyushenko, Viacheslav A1 - Lazarev, Vladimir A. T1 - Fabrication of an antireflection microstructure on AgClBr polycrystalline fiber by single pulse femtosecond laser ablation JF - Optical Materials Express N2 - Polycrystalline infrared (PIR) fibers are used for numerous applications, one of those being power delivery for CO2 lasers. However, the fiber tip surface's transmittance cannot be increased with conventional antireflection coatings due to the surface unevenness. Antireflection microstructures (ARMs) offer an alternative way of increasing transmittance. In this work, ARMs were fabricated on the fiber tip surface of an AgClBr fiber by single-pulse femtosecond laser ablation. A single-surface transmittance of 92.8% at 10.6 μm, a CO2 laser operation wavelength, was achieved. The proposed method can help significantly improve the systems' efficiency, where power delivery for CO2 lasers or sources operating in the wide wavelength range is required. Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-15384 VL - 11 IS - 2 SP - 487 EP - 496 PB - Optical Society of America ER - TY - JOUR A1 - Hauschultz, Mike Thomas A1 - Friedo, Maria Helene A1 - Geißler, Ute A1 - Böhme, Andrea A1 - Krenz-Baath, René T1 - Influence of Process Parameters Variation on Microstructure and Mechanical Properties of SLM-Printed 316L Stainless Steel JF - Solid State Phenomena N2 - This study presents the characterization of 316L stainless steels fabricated by selective laser melting (SLM), focusing on the influence of printing parameters on microstructure and mechanical properties. The choice of process parameters is crucial for achieving desired material properties, as it directly affects the microstructure and mechanical behavior, which is important when optimizing for potential applications in several fields, such as aerospace and automotive. In this study, different scanning speeds were tested to identify optimal settings, followed by the evaluation of the effects of orientation relative to the build plate and hatching strategies to enhance performance. To assess the impact of these factors, tensile tests, microhardness measurements, and X-ray diffraction (XRD) analyses were conducted. Tensile tests revealed that higher laser scanning speed generally reduces ultimate tensile strength and elongation, likely due to an increase in porosity and a less homogeneous fusion of layers. The analysis of samples printed with different orientation relative to the build plate highlighted a strong mechanical anisotropy, with the samples printed vertically exhibiting lower tensile strength and ductility compared to horizontally printed samples. Microhardness testing further confirmed an anisotropy in material properties. XRD analysis reveals a preferential orientation of austenitic grains depending on building direction. This, in turn, influences the anisotropic behavior. These findings highlight the critical role of process parameters in tailoring the microstructure and mechanical performance of SLM-produced parts, thereby providing insights into the optimization of additive manufacturing for specific applications. KW - 316L stainless steel KW - additive manufacturing KW - parameter optimization KW - selective laser melting Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21048 VL - 383 SP - 75 EP - 80 PB - Trans Tech Publications ER - TY - JOUR A1 - Engels, Andreas A1 - Speck, Sandy A1 - Schlegel, Volker A1 - Jacobs, Hannes A1 - Krenz-Baath, René A1 - Böhme, Andrea T1 - Substrate Tubing Heater Suitable for Large Volume 3D Printing with Extrusion of Thermo-Reversible Hydrogels JF - Key Engineering Materials N2 - The advancement of 3-dimensional printing technology over the past ten years has raised interest in and accessibility to these devices. Due to its consistent growth and demand, 3D printing is becoming a consumer-friendly, reasonably priced craft. Due to technological advancements, it is becoming increasingly integrated into broader fields of science and research, as well as the manufacturing sector. The need for customized solutions is constantly growing across several industries. The 3D extrusion of hydrogels is advancing in the field of biomaterials with a broad spectrum of biomedical applications. Hydrogel extrusion prints heads often use stepper motors or pneumatic pressure systems to push the substrate onto a surface. These techniques are well-suited for materials with high viscosity. While these systems are usually bound to the syringe volume, a refillable reservoir enables them to print above the syringe’s limitations. We developed a low-cost standalone heating system for flexible tubes to control the temperature, hence avoiding jellification and clogging of the tubing system leading to the nozzle of the print head. The system connects to an in-house made peristaltic pump, which forces the e.g. gelatin through the nozzle with low pulsation, enabling us to extrude multiple layers of precise tempered gelatin. The heating system is based on easily available materials and electronic components and does not require expensive tools. KW - biomaterial printing KW - direct ink writing KW - gelatine extrusion KW - hydrogel extrusion KW - substrate tube heater Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21092 VL - 1038 SP - 25 EP - 30 PB - Trans Tech Publications ER - TY - JOUR A1 - Werner, Ronald A1 - Prosposito, Paolo A1 - Böhme, Andrea A1 - Krenz-Baath, René T1 - Optimization of Hydrogen Alarm Sensor on Semiconductor Basis JF - Key Engineering Materials N2 - A semiconductor based hydrogen sensor system was optimized by various modifications, which allow an improved detection of very low concentrations of hydrogen in air. The foundation for new investigations on the sensor structure are modifications of substrate and gate structures. Establishment of reference structures is a major aim. A possible drift compensation could be the use of aluminum or alloys for sensor system in order to stabilize signal in Metal Oxide Semiconductor (MOS) respectively Metal Electrolyte Insulator Semiconductor (MEIS) structures. Gold is more likely not capable to function for drift compensation as a pure metal. Nafion™ treatment for cover up the palladium gate seems not to be suitable as a reference, besides could be an option to stabilize sensor signal responses and protect sensors from environmental influences. KW - alarm sensor KW - hydrogen sensor KW - optimized structure KW - semiconductor sensor Y1 - 2026 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21104 VL - 1039 SP - 9 EP - 14 PB - Trans Tech Publications ER -