TY - CHAP A1 - Rösch, Olga ED - Rösch, Olga T1 - Gibt es ein Medium im Deutschen der Gegenwart? T2 - Neue Fragen der Linguistik N2 - Das deutsche verbale Genussystem ist in der Grammatikschreibung zweigliedrig. Den grammatischen Verbformen Aktiv und Passiv werden entsprechend aktivische und passivische Bedeutungen zugeordnet, und somit gelten die semantischen Kategorien Aktiv und Passiv im Gegenwartdeutsch als grammatikalisiert. Der semantischen Kategorie Medium entsprechen im Deutschen keine grammatischen Formparadigmen. Aber auch die Darstellungen des semantischen Begriffes Medium selbst werden in der Literatur eher universalgrammatisch und im engeren Sinne sogar universalsemantisch als einzelsprachlich verstanden. In meiner Untersuchung geht es jedoch nicht primär um das Verständnis des Mediums, sondern um das Verständnis einer bestimmten Konstruktion, die einige Berührungspunkte mit dem früheren Medium aufweist. Die Rede ist von einer formal gekennzeichneten Klasse der Funktionsverbgefüge (FVG), die vielfach als passivische bzw. passivwertige (pw) FVG bezeichnet werden. Y1 - 1991 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12265 SN - 3-484-30270-4 SP - 253 EP - 261 PB - Niemeyer CY - Tübingen ER - TY - JOUR A1 - Hornemann, Andrea A1 - Sinning, Denise A1 - Cortes, Sofia A1 - Campino, Lenea A1 - Emmer, Peggy A1 - Kuhls, Katrin A1 - Ulm, Gerhard A1 - Frohme, Marcus A1 - Beckhoff, Burkhard T1 - A pilot study on fingerprinting Leishmania species from the Old World using Fourier transform infrared spectroscopy JF - Analytical and Bioanalytical Chemistry N2 - Leishmania species are protozoan parasites and the causative agents of leishmaniasis, a vector borne disease that imposes a large health burden on individuals living mainly in tropical and subtropical regions. Different Leishmania species are responsible for the distinct clinical patterns, such as cutaneous, mucocutaneous, and visceral leishmaniasis, with the latter being potentially fatal if left untreated. For this reason, it is important to perform correct species identification and differentiation. Fourier transform infrared spectroscopy (FTIR) is an analytical spectroscopic technique increasingly being used as a potential tool for identification of microorganisms for diagnostic purposes. By employing mid-infrared (MIR) spectral data, it is not only possible to assess the chemical structures but also to achieve differentiation supported by multivariate statistic analysis. This work comprises a pilot study on differentiation of Leishmania species of the Old World (L. major, L. tropica, L. infantum, and L. donovani) as well as hybrids of distinct species by using vibrational spectroscopic fingerprints. Films of intact Leishmania parasites and their deoxyribonucleic acid (DNA) were characterized comparatively with respect to their biochemical nature and MIR spectral patterns. The strains’ hyperspectral datasets were multivariately examined by means of variance-based principal components analysis (PCA) and distance-based hierarchical cluster analysis (HCA). With the implementation of MIR spectral datasets we show that a phenotypic differentiation of Leishmania at species and intra-species level is feasible. Thus, FTIR spectroscopy can be further exploited for building up spectral databases of Leishmania parasites in view of high-throughput analysis of clinical specimens. KW - Fourier transform infrared spectroscopy KW - hierarchical cluster analysis (HCA) KW - principal components analysis (PCA) KW - Leishmania KW - DNA KW - multivariate differentiation Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10080 SN - 1432-1130 ER - TY - JOUR A1 - Lux, Helge A1 - Edling, Matthias A1 - Siemroth, Peter A1 - Schrader, Sigurd T1 - Fast and Cost-Effective Synthesis of High-Quality Graphene on Copper Foils Using High-Current Arc Evaporation JF - Materials N2 - In this paper, we present an innovative and ultra-fast process for the deposition of high-quality graphene on different metal foils and thin metal films. The graphene layer can be homogeneously deposited in only 30 s process time. Due to the weak adhesion to the substrate material, the monolayer graphene is easy to transfer using the established processes. For the production, we use magnetic filtered high-current arc evaporation (Φ-HCA) with a solid, graphitic carbon source. This ultra-fast growth process can pave the way towards a cost-effective graphene synthesis for the mass production e.g., in a roll-to-roll process, avoiding time consuming established processes. KW - graphene KW - PVD KW - arc evaporation KW - copper KW - transfer KW - Φ-HCA Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10359 SN - 1996-1944 VL - 11 IS - 5 SP - 1 EP - 10 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Grebinyk, Sergii A1 - Prylutska, Svitlana A1 - Ritter, Uwe A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification JF - Data in Brief N2 - The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions. This data article refers to the research article “C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al. Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10368 SN - 2352-3409 VL - 19 SP - 2047 EP - 2052 ER - TY - JOUR A1 - Rösch, Olga A1 - Tolkiehn, Günter-Ulrich T1 - Zum Diskurs über die Sprache in der Wissenschaftskommunikation JF - Die neue Hochschule : DNH N2 - Der Diskurs über die Wissenschaftssprache darf nicht von außerwissenschaftlichen Interessen bestimmt werden. Er muss in den Bereich der Wissenschaft zurückgeholt und dort freiheitlich mit wissenschaftlicher Rationalität geführt werden. KW - Sprache KW - Lehrsprache KW - Wissenschaftssprache KW - Wissenschaftsfreiheit KW - Technologietransfer Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10465 SN - 0340-448X IS - 4 SP - 26 EP - 29 ER - TY - JOUR A1 - Fischbach, Jens A1 - Frohme, Marcus A1 - Glökler, Jörn T1 - Hinge-initiated Primer-dependent Amplification of Nucleic Acids (HIP) – A New Versatile Isothermal Amplification Method JF - Scientific Reports N2 - The growing demand for cost-effective nucleic acid detection assays leads to an increasing number of different isothermal amplification reaction methods. However, all of the most efficient methods suffer from highly complex assay conditions due to the use of complicated primer sets and/or auxiliary enzymes. The present study describes the application of a new linker moiety that can be incorporated between a primer and a secondary target binding site which can act both as a block to polymerase extension as well as a hinge for refolding. This novel “hinge-primer” approach results in an efficient regeneration of the primer binding site and thus improves the strand-displacement and amplification process under isothermal conditions. Our investigations revealed that the reaction with forward and reverse hinge-primer including an abasic site is very efficient. The assay complexity can be reduced by combining the hinge-primer with a corresponding linear primer. Furthermore, the reaction speed can be increased by reducing the length of the amplified target sequence. We tested the sensitivity down to 104 copies and found a linear correlation between reaction time and input copy number. Our approach overcomes the usually cumbersome primer-design and extends the range of isothermal amplification methods using a polymerase with strand-displacement activity. KW - DNA synthesis KW - pathogen Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9824 SN - 2045-2322 VL - 7 ER - TY - CHAP A1 - Marc, Goerigk A1 - Liebchen, Christian ED - D'Angelo, Gianlorenzo ED - Dollevoet, Twan T1 - An Improved Algorithm for the Periodic Timetabling Problem T2 - 17th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2017) N2 - We consider the computation of periodic timetables, which is a key task in the service design process of public transportation companies. We propose a new approach for solving the periodic timetable optimisation problem. It consists of a (partially) heuristic network aggregation to reduce the problem size and make it accessible to standard mixed-integer programming (MIP) solvers. We alternate the invocation of a MIP solver with the well-known problem specific modulo network simplex heuristic (ModSim). This iterative approach helps the ModSim-method to overcome local minima efficiently, and provides the MIP solver with better initial solutions. Our computational experiments are based on the 16 railway instances of the PESPlib, which is the only currently available collection of periodic event scheduling problem instances. For each of these instances, we are able to reduce the objective values of previously best known solutions by at least 10.0%, and up to 22.8% with our iterative combined method. KW - periodic timetabling KW - railway optimisation KW - modulo network simplex KW - periodic event scheduling problem Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9899 SN - 978-3-95977-042-2 SP - 12:1 EP - 12:14 PB - Schloss Dagstuhl / Leibniz-Zentrum für Informatik CY - Dagstuhl ER - TY - JOUR A1 - Karakuş, Mehmet A1 - Nasereddin, Abed A1 - Onay, Hüseyin A1 - Karaca, Emin A1 - Özkeklikçi, Ahmet A1 - Jaffe, Charles L. A1 - Kuhls, Katrin A1 - Özbilgin, Ahmet A1 - Ertabaklar, Hatice A1 - Demir, Samiye A1 - Özbel, Yusuf A1 - Töz, Seray T1 - Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT) JF - PLoS Neglected Tropical Diseases N2 - Turkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Şanlıurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Şanlıurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies. Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-9313 SN - 1935-2735 VL - 11 IS - 4 ER - TY - JOUR A1 - Pfeil, Juliane A1 - Frohme, Marcus A1 - Schulze, Katja T1 - Mobile microscopy for the examination of blood samples JF - EMBnet.journal N2 - The analysis of blood is one of the best possibilities to diagnose and control diseases and deficiency symptoms. Common blood tests that are performed in medical laboratories are time-consuming and work-intensive. In under-developed areas, there is often also a lack of specialised staff and facilities. The development of a mobile microscopic system that contains an automated image analysis and that can be used via a smartphone, could represent a valuable help to improve the diagnostic care, especially in those areas. it aims to enable a very fast, cheap, location- and knowledge-independent application for many use cases. Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10509 SN - 2226-6089 VL - 23 SP - e904 ER - TY - JOUR A1 - Gerhard, Christoph A1 - Gimpel, Thomas A1 - Tasche, Daniel A1 - Koch, Jennifer A1 - Brückner, Stephan A1 - Flachenecker, Günter A1 - Wieneke, Stephan A1 - Schade, Wolfgang A1 - Viöl, Wolfgang T1 - Atmospheric pressure plasma-assisted femtosecond laser engraving of aluminium JF - Journal of Physics D: Applied Physics N2 - In this contribution, we report on the impact of direct dielectric barrier discharge argon plasma at atmospheric pressure on femtosecond laser engraving of aluminium. It is shown that the assisting plasma strongly affects the surface geometry and formation of spikes of both laser-engraved single lines and patterns of adjacent lines with an appropriate overlap. Further, it was observed that the overall ablation depth is significantly increased in case of large-scale patterning whereas no notable differences in ablation depth are found for single lines. Several possible mechanisms and underlying effects of this behaviour are suggested. The increase in ablation depth is supposed to be due to a plasma-induced removal of debris particles from the cutting point via charging and oxidation as supported by EDX analysis of the re-solidified debris. Furthermore, the impact of a higher degree of surface wrinkling as well as direct interactions of plasma species with the aluminium surface on the ablation process are discussed. Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10521 SN - 1361-6463 VL - 51 SP - 1 EP - 7 ER - TY - CHAP A1 - Borndörfer, Ralf A1 - Karbstein, Marika A1 - Liebchen, Christian A1 - Lindner, Niels ED - Borndörfer, Ralf ED - Storandt, Sabine T1 - A Simple Way to Compute the Number of Vehicles That Are Required to Operate a Periodic Timetable T2 - 18th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2018) N2 - We consider the following planning problem in public transportation: Given a periodic timetable, how many vehicles are required to operate it? In [Julius Paetzold et al., 2017], for this sequential approach, it is proposed to first expand the periodic timetable over time, and then answer the above question by solving a flow-based aperiodic optimization problem. In this contribution we propose to keep the compact periodic representation of the timetable and simply solve a particular perfect matching problem. For practical networks, it is very much likely that the matching problem decomposes into several connected components. Our key observation is that there is no need to change any turnaround decision for the vehicles of a line during the day, as long as the timetable stays exactly the same. KW - vehicle scheduling KW - periodic timetabling KW - bipartite matching Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10549 SN - 978-3-95977-096-5 SP - 16:1 EP - 16:15 PB - Schloss Dagstuhl - Leibniz-Zentrum für Informatik GmbH CY - Wadern ER -