TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Chepurna, Oksana A1 - Grebinyk, Sergii A1 - Prylutskyy, Yuriy A1 - Ritter, Uwe A1 - Ohulchanskyy, Tymish Y. A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex JF - Nanomaterials N2 - A nanosized drug complex was explored to improve the efficiency of cancer chemotherapy, complementing it with nanodelivery and photodynamic therapy. For this, nanomolar amounts of a non-covalent nanocomplex of Doxorubicin (Dox) with carbon nanoparticle C60 fullerene (C60) were applied in 1:1 and 2:1 molar ratio, exploiting C60 both as a drug-carrier and as a photosensitizer. The fluorescence microscopy analysis of human leukemic CCRF-CEM cells, in vitro cancer model, treated with nanocomplexes showed Dox’s nuclear and C60’s extranuclear localization. It gave an opportunity to realize a double hit strategy against cancer cells based on Dox’s antiproliferative activity and C60’s photoinduced pro-oxidant activity. When cells were treated with 2:1 C60-Dox and irradiated at 405 nm the high cytotoxicity of photo-irradiated C60-Dox enabled a nanomolar concentration of Dox and C60 to efficiently kill cancer cells in vitro. The high pro-oxidant and pro-apoptotic efficiency decreased IC50 16, 9 and 7 × 103-fold, if compared with the action of Dox, non-irradiated nanocomplex, and C60’s photodynamic effect, correspondingly. Hereafter, a strong synergy of therapy arising from the combination of C60-mediated Dox delivery and C60 photoexcitation was revealed. Our data indicate that a combination of chemo- and photodynamic therapies with C60-Dox nanoformulation provides a promising synergetic approach for cancer treatment. KW - photodynamic chemotherapy KW - synergistic effect KW - C60 fullerene KW - doxorubicin KW - nanocomplex KW - leukemic cell KW - apoptosis Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12940 SN - 2079-4991 VL - 9 IS - 11 PB - MDPI ER - TY - JOUR A1 - Rösch, Olga A1 - Tolkiehn, Günter-Ulrich A1 - Lehnert, Ralph T1 - Die Landessprache in der Lehre – welche Bedeutung kommt ihr bei der Internationalisierung zu? JF - Die neue Hochschule : DNH N2 - Der Artikel setzt sich kritisch mit dem Trend auseinander, ganze Studiengänge von Landessprache auf Englisch umzustellen und dies als "Internationaliserung" zu bezeichnen. KW - Landessprache KW - Lehre KW - Englisch KW - Internationalisierung Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12984 SN - 0340-488X VL - 2019 IS - 6 SP - 12 EP - 15 ER - TY - JOUR A1 - Lux, Helge A1 - Edling, Matthias A1 - Lucci, Massimiliano A1 - Kitzmann, Julia A1 - Villringer, Claus A1 - Siemroth, Peter A1 - De Matteis, Fabio A1 - Schrader, Sigurd T1 - The Role of Substrate Temperature and Magnetic Filtering for DLC by Cathodic Arc Evaporation JF - Coatings N2 - Diamond-like carbon (DLC) films were deposited using two different types of high current arc evaporation. The first process used a magnetic particle filter to remove droplets from the plasma. For the second process, the samples were put into a metallic cage which was placed directly above the plasma source. For both processes, we varied the substrate temperature from 21 to 350 °C in order to investigate the temperature effect. The samples were characterized using SEM, AFM, XPS, Raman Spectroscopy, Ellipsometry, Photometry, and Nano Indentation in order to compare both methods of deposition and provide a careful characterization of such DLC films. We found that the sp3 content and the hardness can be precisely adjusted by changing the substrate temperature. Furthermore, in the case of unfiltered deposition, the optical constants can be shifted in the direction of higher absorbance in order to produce black and hard carbon coatings. KW - DLC KW - ta-C KW - arc evaporation KW - substrate temperature KW - hardness KW - optical constant KW - ellipsometry Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12295 SN - 2079-6412 VL - 9 IS - 5 SP - 345 ER - TY - JOUR A1 - Steglich, Patrick A1 - Villringer, Claus A1 - Dietzel, Birgit A1 - Mai, Christian A1 - Schrader, Sigurd A1 - Casalboni, Mauro A1 - Mai, Andreas T1 - On-Chip Dispersion Measurement of the Quadratic Electro-Optic Effect in Nonlinear Optical Polymers Using a Photonic Integrated Circuit Technology JF - IEEE Photonics Journal N2 - A novel method to determine the dispersion of the quadratic electro-optic effect in nonlinear optical materials by using a silicon-on-insulator microring resonator is presented. The microring consists of a silicon slot waveguide enabling large dc electric field strength at low applied voltages. The dispersion of third-order hyperpolarizability of a linear conjugated dye is approximated by using a two-level model for the off-resonant spectral region. As an example, the dispersion of the resonance wavelength of the resonator filled with a dye doped polymer was measured in dependence of the applied dc voltage. The polymer was poly (methylmethacrylate) doped with 5 wt% disperse red 1 (DR1), and the measurements have been carried out at the telecommunication wavelength band around 1550 nm (optical C-band). The described measurements represent a new technique to determine the dispersion of the third-order susceptibility and molecular hyperpolarizability of the material filled into the slot of the ring-resonator. KW - electro-optical device KW - nonlinear optics KW - integrated optics KW - integrated optics material KW - optical material KW - integrated optics device KW - polymer active device Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12307 SN - 1943-0655 VL - 11 IS - 3 ER - TY - JOUR A1 - Mikayelyan, Eduard A1 - Grodd, Linda A1 - Ksianzou, Viachaslau A1 - Wesner, Daniel A1 - Rodygin, Alexander I. A1 - Schönherr, Holger A1 - Luponosov, Yuriy N. A1 - Ponomarenko, Sergei A. A1 - Ivanov, Dimitri A. A1 - Pietsch, Ullrich A1 - Grigorian, Souren T1 - Phase Transitions and Formation of a Monolayer-Type Structure in Thin Oligothiophene Films: Exploration with a Combined In Situ X-ray Diffraction and Electrical Measurements JF - Nanoscale Research Letters N2 - A combination of in situ electrical and grazing-incidence X-ray diffraction (GIXD) is a powerful tool for studies of correlations between the microstructure and charge transport in thin organic films. The information provided by such experimental approach can help optimizing the performance of the films as active layers of organic electronic devices. In this work, such combination of techniques was used to investigate the phase transitions in vacuum-deposited thin films of a common organic semiconductor dihexyl-quarterthiophene (DH4T). A transition from the initial highly crystalline phase to a mesophase was detected upon heating, while only a partial backward transition was observed upon cooling to room temperature. In situ electrical conductivity measurements revealed the impact of both transitions on charge transport. This is partly accounted for by the fact that the initial crystalline phase is characterized by inclination of molecules in the plane perpendicular to the π-π stacking direction, whereas the mesophase is built of molecules tilted in the direction of π-π stacking. Importantly, in addition to the two phases of DH4T characteristic of the bulk, a third interfacial substrate-stabilized monolayer-type phase was observed. The existence of such interfacial structure can have important implications for the charge mobility, being especially favorable for lateral two-dimensional charge transport in the organic field-effect transistors geometry. KW - thin film KW - interfacial monolayer KW - oligomer KW - quarterthiophene KW - in situ GIXD KW - phase transition KW - mesophase Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12314 SN - 1556-276X VL - 14 ER - TY - JOUR A1 - Zhao, Shuang A1 - Caruso, Frank A1 - Dähne, Lars A1 - Decher, Gero A1 - De Geest, Bruno G. A1 - Fan, Jinchen A1 - Feliu, Neus A1 - Gogotsi, Yury A1 - Hammond, Paula T. A1 - Hersam, Mark C. A1 - Khademhosseini, Ali A1 - Kotov, Nicholas A1 - Leporatti, Stefano A1 - Li, Yan A1 - Lisdat, Fred A1 - Liz-Marzán, Luis M. A1 - Moya, Sergio A1 - Mulvaney, Paul A1 - Rogach, Andrey L. A1 - Roy, Sathi A1 - Shchukin, Dmitry G. A1 - Skirtach, Andre G. A1 - Stevens, Molly M. A1 - Sukhorukov, Gleb B. A1 - Weiss, Paul S. A1 - Yue, Zhao A1 - Zhu, Dingcheng A1 - Parak, Wolfgang J. T1 - The Future of Layer-by-Layer Assembly: A Tribute to ACS Nano Associate Editor Helmuth Möhwald JF - ACS Nano N2 - Layer-by-layer (LbL) assembly is a widely used tool for engineering materials and coatings. In this Perspective, dedicated to the memory of ACS Nano associate editor Prof. Dr. Helmuth Möhwald, we discuss the developments and applications that are to come in LbL assembly, focusing on coatings, bulk materials, membranes, nanocomposites, and delivery vehicles. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12356 SN - 1936-086X VL - 13 IS - 6 SP - 6151 EP - 6169 ER - TY - JOUR A1 - Kober, Liane A1 - Hollert, Henner A1 - Frohme, Marcus T1 - Quantification of nitroaromatic explosives in contaminated soil using MALDI-TOF mass spectrometry JF - Analytical and Bioanalytical Chemistry N2 - Contamination from various sources is a global environmental and health threat, with mining and military activities in particular having spread nitroaromatic compounds, such as 2,4,6-trinitrotoluene and its degradation products and by-products, to the soil. The investigation and monitoring of large contaminated areas requires new detection methods since the established ones are expensive and time-consuming. Hence, we established a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI--TOF MS) method using 1,5-diaminonaphthalene as the matrix substance and an internal standard for quantification. Analyzing standard substances, we found specific signals for radical and fragment ions of different nitrotoluenes and nitrobenzenes with good reproducibility and detection limits down to 0.25 ng/μL. The analysis of soil sample extracts from a former production site showed clear signals for 2,4,6-trinitrotoluene and the primary degradation products aminodinitrotoluenes. Furthermore, quantification gave results comparable to those obtained by conventional liquid chromatography--tandem mass spectrometry analysis. The MALDI-TOF MS method has a comparatively lower reproducibility, with relative standard deviations of 6% to 20% for multiple measurements of standard solutions and soil sample extracts. Nevertheless, a comparison of both methods revealed the advantages of MALDI-TOF MS analysis of explosive-contaminated areas with regard to costs, time, and handling. Finally, our MALDI-TOF MS method fulfills all the needs for high sample throughput and can therefore be a valuable screening tool for explosive-contaminated areas. KW - explosives KW - soil contamination KW - TNT KW - matrix-assisted laser desorption/ionization quantification Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12388 SN - 1618-2650 VL - 411 IS - 23 SP - 5993 EP - 6003 ER - TY - JOUR A1 - Bondarenko, Siegfried A1 - Villringer, Claus A1 - Steglich, Patrick T1 - Comparative Study of Nano-Slot Silicon Waveguides Covered by Dye Doped and Undoped Polymer Cladding JF - Applied Sciences N2 - Nonlinear optical dyes doped in optical polymer matrices are widely used for electro-optical devices. Linear optical properties change with dye concentration, which leads to a change in modal properties, especially in nano-structured integrated waveguides such as silicon slot-waveguides. Here, we investigate the influence of a nonlinear optical dye on the performance of a silicon-organic hybrid slot-waveguide. A simulation study of the modal and optical confinement properties is carried out and dependence of the structural parameters of the slot-waveguide and the organic cladding material is taken into account. As cladding material, a guest-host polymer system is employed comprising the nonlinear optical dye Disperse Red 1 (DR1) doped in a poly[methyl methacrylate] (PMMA) matrix. The refractive indices of doped and undoped PMMA were deduced from ellipsometric data. We present a guideline for an optimized slot-waveguide design for the fabrication in silicon-on-insulator technology giving rise to scalable, high-performance integrated electro-optical modulators. KW - silicon photonics KW - modeling and simulation at the nanoscale KW - nonlinear optics at the nanoscale KW - slot waveguide KW - silicon-on-insulator technology Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10716 SN - 2076-3417 VL - 9 IS - 1 PB - MDPI ER - TY - JOUR A1 - Steglich, Patrick A1 - Hülsemann, Marcel A1 - Dietzel, Birgit A1 - Mai, Andreas T1 - Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review JF - Molecules N2 - Recent developments in optical biosensors based on integrated photonic devices are reviewed with a special emphasis on silicon-on-insulator ring resonators. The review is mainly devoted to the following aspects: (1) Principles of sensing mechanism, (2) sensor design, (3) biofunctionalization procedures for specific molecule detection and (4) system integration and measurement set-ups. The inherent challenges of implementing photonics-based biosensors to meet specific requirements of applications in medicine, food analysis, and environmental monitoring are discussed. KW - biosensor KW - biophotonics KW - integrated optical sensor KW - aptamer KW - biomaterial KW - optical sensor KW - silicon photonics KW - ring resonator KW - lab-on-a-chip Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10822 SN - 1420-3049 VL - 24 IS - 3 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Grebinyk, Sergii A1 - Prylutskyy, Yuriy A1 - Ritter, Uwe A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro JF - Nanoscale Research Letters N2 - Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function. The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells. KW - C60 fullerene KW - doxorubicin KW - noncovalent complex KW - leukemic cell KW - cytotoxicity KW - accumulation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10966 SN - 1556-276X N1 - Das Dokument wurde zurückgezogen. Die korrigierte Version ist unter dem folgenden Link zu finden: https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-11415 VL - 14 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Grebinyk, Sergii A1 - Prylutskyy, Yuriy A1 - Ritter, Uwe A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro JF - Nanoscale Research Letters N2 - Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function. The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells. KW - C60 fullerene KW - doxorubicin KW - noncovalent complex KW - leukemic cell KW - cytotoxicity KW - accumulation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11415 SN - 1556-276X VL - 14 ER - TY - JOUR A1 - Ida, Jeunice A1 - Chan, Soo Khim A1 - Glökler, Jörn A1 - Lim, Yee Ying A1 - Choong, Yee Siew A1 - Lim, Theam Soon T1 - G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing JF - Molecules N2 - G-quadruplexes are made up of guanine-rich RNA and DNA sequences capable of forming noncanonical nucleic acid secondary structures. The base-specific sterical configuration of G-quadruplexes allows the stacked G-tetrads to bind certain planar molecules like hemin (iron (III)-protoporphyrin IX) to regulate enzymatic-like functions such as peroxidase-mimicking activity, hence the use of the term DNAzyme/RNAzyme. This ability has been widely touted as a suitable substitute to conventional enzymatic reporter systems in diagnostics. This review will provide a brief overview of the G-quadruplex architecture as well as the many forms of reporter systems ranging from absorbance to luminescence readouts in various platforms. Furthermore, some challenges and improvements that have been introduced to improve the application of G-quadruplex in diagnostics will be highlighted. As the field of diagnostics has evolved to apply different detection systems, the need for alternative reporter systems such as G-quadruplexes is also paramount. KW - G-quadruplex KW - DNAzyme KW - colorimetric KW - fluorescence KW - luminescence KW - diagnostics Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11438 SN - 1420-3049 VL - 24 IS - 6 ER - TY - JOUR A1 - Göbel, Gero A1 - Talke, Anja A1 - Ahnert, Uwe A1 - Lisdat, Fred T1 - Electrochemical Activity Determination of Catechol-O-methyl Transferase by Selective Dopamine Detection JF - ChemElectroChem N2 - For the treatment of Parkinson's disease, as one of the most frequent diseases of the central nervous system, several key enzymes for dopamine metabolism [e. g. catechol-O-methyl transferase (COMT)] are drug targets. For an efficient and long-lasting treatment, the activity of this enzyme should be monitored. In this study, an electrochemical approach using differential pulse voltammetry (DPV) is introduced for the activity determination. The applied electrode material, fluorine-doped tin oxide (FTO), is characterized by a clear discrimination between substrate and product of COMT, a high stability of the dopamine signal during consecutive measurements, and a linear dependency on the dopamine concentration in the range of the maximum reaction rate of COMT. Despite these advantageous results, dopamine detection in the complete activity assay is influenced by each of the added essential assay components, even though none of the added components reveal a current signal at the FTO electrode itself. After adjusting the potential range and the assay composition, these effects can be circumvented. By following the dopamine concentrations during COMT action, it can be shown that the activity of COMT can be detected by using differential pulse voltammetry (DPV) at an FTO electrode and, by analyzing different COMT amounts, quantification can be demonstrated. KW - catechol-O-methyl transferase KW - dopamine KW - flow system KW - fluorine-doped tin oxide KW - sensing Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13900 VL - 6 IS - 17 SP - 4533 EP - 4540 PB - Wiley ER - TY - CHAP A1 - Lindner, Niels A1 - Liebchen, Christian T1 - New Perspectives on PESP: T-Partitions and Separators T2 - 19th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2019) N2 - In the planning process of public transportation companies, designing the timetable is among the core planning steps. In particular in the case of periodic (or cyclic) services, the Periodic Event Scheduling Problem (PESP) is well-established to compute high-quality periodic timetables. We are considering algorithms for computing good solutions and dual bounds for the very basic PESP with no additional extra features as add-ons. The first of these algorithms generalizes several primal heuristics that have been proposed, such as single-node cuts and the modulo network simplex algorithm. We consider partitions of the graph, and identify so-called delay cuts as a structure that allows to generalize several previous heuristics. In particular, when no more improving delay cut can be found, we already know that the other heuristics could not improve either. This heuristic already had been proven to be useful in computational experiments [Ralf Borndörfer et al., 2019], and we locate it in the more general concept of what we denote T-partitions. With the second of these algorithms we propose to turn a strategy, that has been discussed in the past, upside-down: Instead of gluing together the network line-by-line in a bottom-up way, we develop a divide-and-conquer-like top-down approach to separate the initial problem into two easier subproblems such that the information loss along their cutset edges is as small as possible. We are aware that there may be PESP instances that do not fit well the separator setting. Yet, on the RxLy-instances of PESPlib in our experimental computations, we come up with good primal solutions and dual bounds. In particular, on the largest instance (R4L4), this new separator approach, which applies a state-of-the-art solver as subroutine, is able to come up with better dual bounds than purely applying this state-of-the-art solver in the very same time. KW - periodic event scheduling problem KW - periodic timetabling KW - graph partitioning KW - graph separators KW - balanced cuts Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-20074 SP - 2:1 EP - 2:18 PB - Schloss Dagstuhl, Leibniz-Zentrum für Informatik CY - Dagstuhl ER - TY - JOUR A1 - Arango Pérez, Andrés T1 - Implementing Augmented Reality in the Flight Deck for Single Pilot Operations JF - Anwendungen und Konzepte der Wirtschaftsinformatik N2 - Single Pilot Operations is a current topic with the potential to significantly affect the future of commercial aviation. While financially attractive for airlines, Single Pilot Operations bring forth important safety concerns, especially regarding the lack of human redundancy in the flight deck, an increased workload for the single pilot, reduced situational awareness and a higher risk of human error. It is assumed that potential problems affecting Single Pilot Operations could be addressed by implementing an Augmented Reality (AR) device in the flight deck, by presenting additional information and supporting hints within the pilot’s field of view. Concretely, AR could be used to help reduce the single pilot’s workload, improve situational awareness and reduce the risk of human error. This paper sets out to demonstrate two use cases for augmented reality in the flight deck. A system, called Pilot Assist, was developed that allows pilots to conduct checklists interactively with a Microsoft HoloLens. The system also provides a holographic Head-up-Display. Pilot Assist was developed and demonstrated with a fixed base Airbus A320 simulator at the Technical University of Wildau. With the HoloLens’ spatial mapping capabilities – scanning and recognizing the environment around the user – it was possible to create a system that guides the pilot through the conduction of checklists. This is done by prompting the user towards the location of each checklist item in the cockpit, where information regarding necessary actions is projected. Furthermore, Pilot Assist is integrated with the aircraft systems, making it possible to obtain aircraft status data in real time, thus allowing error-checking of the pilot’s actions as well as automating the progress through checklists. The holographic Head-up-Display allows the user to look at the surrounding environment while presenting critical flight data within the user’s field of view. The holographic Head-up-Display is intended to contribute to the pilot’s situational awareness. Experts in the aviation field, including pilots, researchers and engineers had the chance to qualitatively assess the Pilot Assist tool. They pointed to limitations of both Pilot Assist and the HoloLens itself, but shared optimism as to how this technology and similar applications could indeed impact the future of flight operations. Concerns regarding the HoloLens’ weight, comfort and narrow field of view were expressed. However, continued development of head mounted devices (e.g. HoloLens 2) is expected in the coming years. Further research into augmented reality applications in the flight deck is needed to advance this and other use cases. Nonetheless, the experts agreed Pilot Assist provides beneficial support during single pilot operation considering the current prototypical nature of the system. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16649 VL - 9 SP - 60 EP - 72 PB - Arbeitskreis Wirtschaftsinformatik (AKWI) ER - TY - JOUR A1 - Peuschke-Bischof, Tobias T1 - Der Mensch als Einflussfaktor beim manuellen Fahren JF - Anwendungen und Konzepte der Wirtschaftsinformatik N2 - Dieser Ausschnitt aus einer Masterarbeit dient der Betrachtung der Aufnahme und Verarbeitung von Informationen durch den Menschen im Straßenverkehr. Dazu werden unter anderem die Prozesse der Informationsaufnahme, Informationsverarbeitung und die Überführung der Informationen in eine Handlung genauer betrachtet, um erkennen zu können, welchen Nutzen Fahrerassistenzsysteme erzielen können, indem sie dem Menschen die Fahraufgabe entweder teilweise oder sogar vollständig abnehmen. KW - Informationsverarbeitung KW - Manuelles Fahren KW - Autonomes Fahren KW - Fahrerassistenzsystem KW - Wahrnehmung KW - salience effort expectancy value model KW - Situationsbewusstsein KW - extended control model Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16655 VL - 9 SP - 83 EP - 87 PB - Arbeitskreis Wirtschaftsinformatik (AKWI) ER - TY - BOOK ED - Steglich, Patrick ED - De Matteis, Fabio T1 - Fiber Optics - From Fundamentals to Industrial Applications N2 - Optical fibers in metrology, telecommunications, sensors, manufacturing, and health science have gained massive research interest. The number of applications is increasing at a fast pace. This book aims to present a collection of recent advances in fiber optics, addressing both fundamental and industrial applications. It covers the current progress and latest breakthroughs in emergent applications of fiber optics. The book includes five chapters on recent developments in optical fiber communications and fiber sensors, as well as the design, simulation, and fabrication of novel fiber concepts. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18093 UR - https://www.intechopen.com/books/7294 SN - 978-1-83881-157-0 PB - IntechOpen CY - London ER - TY - JOUR A1 - Fursenko, Oksana A1 - Lukosius, Mindaugas A1 - Bauer, Joachim A1 - Villringer, Claus A1 - Lux, Helge A1 - Bärwolf, Florian A1 - Lisker, Marco A1 - Mai, Andreas T1 - Diagnostic of graphene on Ge(100)/Si(100) in a 200 mm wafer Si technology environment by spectroscopic ellipsometry/reflectometry JF - Journal of Vacuum Science & Technology B N2 - Comprehensive diagnostics is a prerequisite for the application of graphene in semiconductor technologies. Here, the authors present long-term investigations of graphene on 200-mm Ge(100)/Si(100) wafers under clean room environmental conditions. Diagnostic of graphene was performed by a fast and nondestructive metrology method based on the combination of spectroscopic ellipsometry and reflectometry (SE/R), realized within a wafer optical metrology tool. A robust procedure for unambiguous thickness monitoring of a multilayer film stack, including graphene, interface layer GeOx underneath graphene, and surface roughness is developed and applied for process control. The authors found a relationship between the quality of graphene and the growth of GeOx beneath graphene. Enhanced oxidation of Ge beneath graphene was registered as a long-term process. SE/R measurements were validated and complemented using atomic force microscopy, scanning electron microscopy, Raman spectroscopy, and secondary ion mass spectrometry. This comparative study shows a high potential for optical metrology of graphene deposited on Ge/Si structures, due to its great sensitivity, repeatability, and flexibility, realized in a nondestructive way. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16083 VL - 37 IS - 6 PB - American Vacuum Society (AVS) ER - TY - INPR A1 - Gatto, Emanuela A1 - Kubitzky, Sascha A1 - Schriever, Marc A1 - Cesaroni, Simona A1 - Mazzuca, Claudia A1 - Marafon, Giulia A1 - Venanzi, Mariano A1 - De Zotti, Marta T1 - Building Supramolecular DNA-Inspired Nanowires on Gold Surfaces: From 2D to 3D T2 - Angewandte Chemie International Edition N2 - Three building blocks have been designed to chemically link to a gold surface and vertically self-assemble through thymine–adenine hydrogen bonds. Starting from these building blocks, two different films were engineered on gold surface. Film 1 consists of adenine linked to lipoic acid (Lipo–A) to covalently bind to the gold surface, and ZnTPP linked to a thymine (T–ZnTPP). Film 2 has an additional noncovalently linked layer: a helical undecapeptide analogue of the trichogin GA IV peptide, in which four glycines were replaced by four lysines to favor a helical conformation and reduce flexibility and the two extremities were functionalized with thymine and adenine to enable Lipo–A and T–ZnTPP binding, respectively. These films were characterized by electrochemical and spectroscopic techniques, and were very stable over time and when in contact with solution. Under illumination, they could generate current with higher efficiency than similar previously described systems. KW - peptide KW - photocurrent generation KW - self-assembled monolayer KW - self-assembly KW - supramolecular chemistry Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16113 PB - Wiley ER - TY - CHAP A1 - Villringer, Claus A1 - Gilani, Taravat Saeb A1 - Zhang, Edward A1 - Pulwer, Silvio A1 - Steglich, Patrick A1 - Schrader, Sigurd A1 - Laufer, Jan T1 - Development of tuneable Fabry-Pérot sensors for parallelised photoacoustic signal acquisition T2 - Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108780M (8 March 2019) N2 - Fabry-Pérot (FP) sensors have enabled high resolution 3D photoacoustic (PA) imaging in backward mode. However, raster-scanning of the interrogation laser beam across the sensor can result in slow 3D image acquisition. To overcome this limitation, parallelized PA signal acquisition can be used for which FP sensors with uniform optical thickness are required. In this work, the optical thickness is tuned a) irreversibly through the use of a photopolymer host matrix and b) actively using embedded electro-optic (EO) chromophores. Polymer spacers (5 μm) were deposited using spin coating and sandwiched between two dielectric mirrors and transparent ITO electrodes. The employed polymer guest-host system consists of an EO chromophore (2-methyl-4-nitroaniline) and poly(vinyl cinnamate). EO tuneability was induced using contact poling and a tuneability of 68 pm was demonstrated. The optical thickness was homogenised by raster scanning a UV beam whilst varying the exposure time across a 4 mm2 detection aperture. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13769 SN - 1996-756X PB - Society of Photo-Optical Instrumentation Engineers (SPIE) ER - TY - JOUR A1 - Momanyi, Job A1 - Herzog, Michael A1 - Muchiri, Peter T1 - Analysis of Thermomechanical Properties of Selected Class of Recycled Thermoplastic Materials Based on Their Applications JF - Recycling N2 - Polypropylene and polystyrene are petroleum-based thermoplastics which are commonly used and disposed of in the environment after their service life, leading to environmental degradation. There is a need to recycle polypropylene and polystyrene, but the effect of recycling on thermo-mechanical properties is not well understood. This study aims to determine thermo-mechanical properties of the recycled polypropylene and recycled polystyrene and compare them with corresponding virgin polypropylene and newly produced polystyrene (general purpose polystyrene 1540 and high impact polystyrene 7240). The study was carried out by preparing bar-shaped samples of recycled polypropylene, recycled polystyrene, general purpose polystyrene 1540, and high impact polystyrene 7240 by compression molding using a hot press and thermally characterizing them to determine glass transition temperature and melting temperature using differential scanning calorimetry. The changes in Young’s modulus, tensile strength, hardness, and toughness due to recycling activities were determined at room temperature (24 °C), 40 °C, 60 °C, and 80 °C. The thermo-mechanical properties of recycled polystyrene (PS) were found to be comparable to those of high impact polystyrene (HIPS) 7240. The study revealed that the hardness and toughness for the recycled polymers were higher than those of corresponding virgin polymers. On the other hand, tensile strength and Young's modulus for the recycled polymers were lower than those of the virgin polymers. Understanding the thermo-mechanical properties of the recycled polymers will contribute to more industrial applications hence increase the rate of recycling, resulting in a reduction in environmental pollution. KW - polymer KW - thermoplastic KW - thermo-mechanical property KW - recycled polypropylene KW - recycled polystyrene Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12509 SN - 2313-4321 VL - 4 ER - TY - JOUR A1 - Sukiasyan, Anna A1 - Keshishyan, Ara A1 - Manukyan, Dezdemonia A1 - Melik-Andreasyan, Gayane A1 - Atshemyan, Liana A1 - Apresyan, Hripsime A1 - Strelkova, Margarita V. A1 - Frohme, Marcus A1 - Cortes, Sofia A1 - Kuhls, Katrin T1 - Re-Emerging Foci of Visceral Leishmaniasis in Armenia – First Molecular Diagnosis of Clinical Samples JF - Parasitology N2 - Visceral leishmaniasis (VL) was firstly reported in Armenia in 1913. Following a considerable increase of the number of cases until the mid 1950s, the diseases disappeared after 1969 and re-emerged in 1999. Scientific literature about VL in Armenia is available only in Russian or Armenian. This paper presents a historical overview about leishmaniasis in Armenia based on this literature as well as an epidemiological update since the re-emergence of the disease. In 1999- 2016, 116 indigenous VL cases were recorded mainly in children in 8 of the 11 districts, however VL is underreported because of lack of trained medical personal and diagnostic facilities. The aim of this work was to apply for the first time molecular diagnosis of VL in Armenia. Out of 25 VL suspected patients, 22 were positive by microscopy and PCR. Genotyping using ITS1-PCR-RFLP and sequencing identified the causative agent of VL in Armenia as Leishmania infantum. The present work is an important step towards the inclusion of molecular techniques in the current diagnosis of VL in Armenia and the establishment of local molecular diagnostic facilities. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12018 SN - 1469-8161 ER - TY - THES A1 - Radke, Lars T1 - Evaluierung der Potenz von glykosylierten Impfstoffen am Beispiel des RSV-F Proteins N2 - Trotz intensiver Forschung und immensen Bedarfs steht heutzutage kein RSV-Impfstoff zur Verfügung. Moderne Subunit-Vakzine bedürfen einer Adjuvantierung, um eine ausreichend starke Immunantwort zu generieren. Mit der Veränderung von Glykoslierungsmustern des RSV-F Proteins wird eine kovalent gekoppelte Adjuvantierung hergestellt, die über die Aktivierung des angeborenen Immunsystems eine umfassende Immunantwort generiert. In dieser Arbeit wurden rekombinant hergestellte Varianten des RSV-F Proteins mit defukosylierten oder xylosylierten N-Glykanen auf ihr Potential, das Immunsystem zu aktivieren, untersucht. Dazu wurden Zytokine aus Zellkulturüberständen quantifiziert und Methoden zur Genexpressionsanalyse stimulierter Zellen etabliert. In Vorbereitung auf Stimulationsexperimente mit PBMCs wurde der Einfluss der Kryokonservierung auf Zellen untersucht und die Zytokinexpression auf Gen- und Proteinebene korreliert. Im Rahmen der Stimulationsexperimente mit komplexen Zellgemischen (PBMC und mDC) wurden die stabil exprimierten Referenzgene SDHA, TBP und PPIA für die Etablierung einer validen Genexpressionsanalyse identifiziert. Dabei wurde eine mathematische Konvertierung quantitativer Transkriptzahlen aus dem Sonden-basierten direkten RNA-Nachweis (NanoString®) und der relativen Quantifizierung mittels qPCR entwickelt. Die Zytokinmuster in Zellkulturüberständen aus Langzeitstimulationsexperimenten, welche mit PBMC, mDC und den beiden Impfstoffvarianten in einem komplexen Perfusionsreaktorsystem (HuALN®) durchgeführt worden waren, wurden mittels automatisierter Bead-basierter Multiplexanalytik quantifiziert. Im Zusammenhang mit der Untersuchung umfangreicher Genpanels konnten verstärkte Immunreaktionen durch die glykosidisch veränderten RSV-F Proteine nachgewiesen werden. Die Defukosylierung führt zu einer breiten Aktivierung des zellulären und humoralen adaptiven Immunsystems. Das xylosylierte RSV-F aktiviert zusätzlich Komponenten des angeborenen Immunsystems. Zudem gibt es Hinweise darauf, dass durch den Grad der Defukosylierung das Verhältnis zwischen Th1 und Th2 basierter Antwort modifiziert werden kann. Mit den verwendeten und etablierten Methoden ist es möglich, die Potenz von Impfstoffkandidaten mit geringen strukturellen Veränderungen zu unterscheiden. Um die komplexen Stimulationsexperimente in einem höheren Durchsatz durchführen zu können, wurde an der Entwicklung eines miniaturisierten Bioreaktors mitgewirkt. Die Untersuchungen der Dichtheit des Reaktors mittels Fluoreszenzmikroskopie und der Biokompatibilität der einzelnen Komponenten hatten direkten Einfluss auf das Design des Reaktors, dessen Aufbau- und Verbindungstechnik sowie die Wahl der Materialien. Die Funktionalität konnte in verschiedenen 2D und 3D Kultivierungsansätzen über eine langanhaltende Zellviabilität bestätigt werden. Die verstärkte Modulierung der Immunantwort, hervorgerufen durch modifizierte Glykanstrukturen, zeugt von einer aussichtsreichen Methode, die auf andere Pathogene angewandt werden kann. Das höhere Durchsatzpotential des miniaturisierten Perfusionsreaktors (mehr Zeitpunkte, mehr Replikate) kann zukünftig einen präziseren Einblick in die immunologischen Vorgänge gewähren. N2 - Despite intense research and immense demand there is no RSV vaccine available today. Modern subunit vaccines require adjuvants to generate a sufficiently strong immune response. By editing the glycosylation pattern of the RSV-F Protein covalently coupled adjuvants are produced, which generate comprehensive immune responses via the activation of the innate immune system. In this work recombinantly produced variants of the RSV-F protein with defucosylated or xylosylated Nglycans were tested for their potential to activate the immune system. For that, cytokines from cell culture supernatants were quantified and methods for gene expression analysis of stimulated cells were established. In preliminary PBMC stimulation experiments the influence of cryopreservation on cells was examined and the expression of cytokines on gene and protein level was correlated. Within the scope of stimulation experiments with complex cell mixtures (PBMC and DC) the reference genes SDHA, TBP and PPIA were identified as stably expressed in order to establish a valid gene expression analysis. A mathematical conversion of quantitative transcript numbers from a probe-based direct RNA detection assay (NanoString®) and the relative quantification via qPCR was developed. The cytokine pattern in cell culture supernatants from long-term experiments performed with PBMC, mDC and each vaccine candidate in a highly complex perfusion reactor system (HuALN®) were quantified by automated bead-based multiplex analytics. In connection with the analysis of broad gene panels increased immune responses could be detected caused by the glycosidically altered RSV-F proteins. Defucosylation leads to a comprehensive activation of the immune system. Xylosylated RSV-F additionally activates components of the innate immune system. Furthermore, there is evidence that the ratio of the Th1 and Th2 based immune response can be modified by the grade of the defucosylation. The applied and established methods are suited to distinguish the potency of vaccine candidates with minor structural differences. In order to perform stimulation experiments in a higher throughput, this work contributed to the establishment of a miniaturized bioreactor. Leakage testing of the reactor via fluorescence microscopy and the examination of the biocompatibility of all single components directly influenced the reactor design, its assembly and connection techniques as well as the choice of materials. The reactors functionality was confirmed in different 2D and 3D cultivation approaches by means of a long-lasting high cell viability. The stronger modulated immune response evoked by modified glycosidic structures provides a promising method that can be applied to other pathogens, too. By its potential for higher throughput (more time points, more replicas) the miniaturized perfusion reactor could be able to provide a more precise insight into the immunological processes. KW - RSV-F KW - Glycosylation KW - vaccine KW - xylosylation KW - defucosylation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14162 ER - TY - CHAP A1 - Root, Konstantin A1 - Wiechers, Henning A1 - Azmitia, Alfredo A1 - Mohnke, Janett A1 - Müller, Christian ED - Barton, Thomas ED - Müller, Christian ED - Seel, Christian T1 - Reorganisation und Optimierung von Prozessen am Beispiel der Verwaltung von Abschlussarbeiten und des Qualitätsmonitorings von Studiengängen T2 - Hochschulen in Zeiten der Digitalisierung : Lehre, Forschung und Organisation N2 - Die internen Abläufe an Hochschulen folgen selten vordefinierten Regeln, sodass bei der Digitalisierung solcher Prozesse Standardlösungen vielfach nicht ausreichen. Für die Abbildung ehemals analoger Prozesse sind individuelle Softwarelösungen unabdingbar. In diesem Kapitel soll am Beispiel ausgewählter hochschulspezifischer Softwareanwendungen der Weg beschrieben werden, wie ein solcher Umbau im Rahmen bestehender Systeme vollzogen werden kann. Im Fokus dieser Beschreibung stehen Softwareanwendungen, die sowohl die Verwaltungsprozesse rund um die Erstellung von Thesisarbeiten – beginnend mit der Anmeldung bis zur Abgabe der Arbeit, der Durchführung von Abschlussprüfungen bis zur Publikation der Thesisarbeiten – als auch Akkreditierungsprozesse an der TH Wildau unterstützen, mit denen Modulhandbücher und Logbücher erstellt werden, die die Entwicklung von Studiengängen zwischen den Reakkreditierungen transparent nachvollziehbar machen. Ein besonderes Augenmerk wird auf Schnittstellen anderer Hochschulprozesse gelegt. Diese Anwendungen wurden mit agilen Methoden erstellt. Das Zusammenspiel von agiler Softwareentwicklung und entsprechenden Evaluationsschritten soll zeigen, wie diese Methoden an der Hochschule mit kleinen Teams effektiv eingesetzt werden können, um mit Blick auf die Verbesserung der Qualität und Einheitlichkeit von Studium und Lehre ein bestmögliches Optimierungspotenzial zu erreichen. KW - Hochschulprozess KW - Thesis KW - Qualitätssicherung KW - Akkreditierung KW - Monitoring KW - Implementierung Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18320 SN - 978-3-658-26617-2 SP - 139 EP - 153 PB - Springer Vieweg CY - Berlin ER - TY - JOUR A1 - de Vera, Jean-Pierre Paul A1 - Alawi, Mashal A1 - Backhaus, Theresa A1 - Baqué, Mickael A1 - Billi, Daniela A1 - Böttger, Ute A1 - Berger, Thomas A1 - Bohmeier, Maria A1 - Cockell, Charles A1 - Demets, René A1 - de la Torre Noetzel, Rosa A1 - Edwards, Howell A1 - Elsaesser, Andreas A1 - Fagliarone, Claudia A1 - Fiedler, Annelie A1 - Foing, Bernard A1 - Foucher, Frédéric A1 - Fritz, Jörg A1 - Hanke, Franziska A1 - Herzog, Thomas H. A1 - Horneck, Gerda A1 - Hübers, Heinz-Wilhelm A1 - Huwe, Björn A1 - Joshi, Jasmin A1 - Kozyrovska, Natalia A1 - Kruchten, Martha A1 - Lasch, Peter A1 - Lee, Natuschka A1 - Leuko, Stefan A1 - Leya, Thomas A1 - Lorek, Andreas A1 - Martínez-Frías, Jesús A1 - Meessen, Joachim A1 - Moritz, Sophie A1 - Moeller, Ralf A1 - Olsson-Francis, Karen A1 - Onofri, Silvano A1 - Ott, Sieglinde A1 - Pacelli, Claudia A1 - Podolich, Olga A1 - Rabbow, Elke A1 - Reitz, Günther A1 - Rettberg, Petra A1 - Reva, Oleg A1 - Rothschild, Lynn A1 - Garcia Sancho, Leo A1 - Schulze-Makuch, Dirk A1 - Selbmann, Laura A1 - Serrano, Paloma A1 - Szewzyk, Ulrich A1 - Verseux, Cyprien A1 - Wadsworth, Jennifer A1 - Wagner, Dirk A1 - Westall, Frances A1 - Wolter, David A1 - Zucconi, Laura T1 - Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS JF - Astrobiology N2 - BIOMEX (BIOlogy and Mars EXperiment) is an ESA/Roscosmos space exposure experiment housed within the exposure facility EXPOSE-R2 outside the Zvezda module on the International Space Station (ISS). The design of the multiuser facility supports—among others—the BIOMEX investigations into the stability and level of degradation of space-exposed biosignatures such as pigments, secondary metabolites, and cell surfaces in contact with a terrestrial and Mars analog mineral environment. In parallel, analysis on the viability of the investigated organisms has provided relevant data for evaluation of the habitability of Mars, for the limits of life, and for the likelihood of an interplanetary transfer of life (theory of lithopanspermia). In this project, lichens, archaea, bacteria, cyanobacteria, snow/permafrost algae, meristematic black fungi, and bryophytes from alpine and polar habitats were embedded, grown, and cultured on a mixture of martian and lunar regolith analogs or other terrestrial minerals. The organisms and regolith analogs and terrestrial mineral mixtures were then exposed to space and to simulated Mars-like conditions by way of the EXPOSE-R2 facility. In this special issue, we present the first set of data obtained in reference to our investigation into the habitability of Mars and limits of life. This project was initiated and implemented by the BIOMEX group, an international and interdisciplinary consortium of 30 institutes in 12 countries on 3 continents. Preflight tests for sample selection, results from ground-based simulation experiments, and the space experiments themselves are presented and include a complete overview of the scientific processes required for this space experiment and postflight analysis. The presented BIOMEX concept could be scaled up to future exposure experiments on the Moon and will serve as a pretest in low Earth orbit. KW - EXPOSE-R2 KW - BIOMEX KW - habitability KW - limits of life KW - extremophiles KW - Mars Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12048 SN - 1557-8070 VL - 19 IS - 2 ER - TY - JOUR A1 - Kagel, Heike A1 - Bier, Frank Fabian A1 - Frohme, Marcus A1 - Glökler, Jörn T1 - A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids JF - Scientific Reports N2 - Most biochemical reactions depend on the pH value of the aqueous environment and some are strongly favoured to occur in an acidic environment. A non-invasive control of pH to tightly regulate such reactions with defined start and end points is a highly desirable feature in certain applications, but has proven difficult to achieve so far. We report a novel optical approach to reversibly control a typical biochemical reaction by changing the pH and using acid phosphatase as a model enzyme. The reversible photoacid G-acid functions as a proton donor, changing the pH rapidly and reversibly by using high power UV LEDs as an illumination source in our experimental setup. The reaction can be tightly controlled by simply switching the light on and off and should be applicable to a wide range of other enzymatic reactions, thus enabling miniaturization and parallelization through non-invasive optical means. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12685 SN - 2045-2322 VL - 9 ER - TY - JOUR A1 - Kagel, Heike A1 - Jacobs, Hannes A1 - Bier, Frank Fabian A1 - Glökler, Jörn A1 - Frohme, Marcus T1 - A Novel Microtiter Plate Format High Power Open Source LED Array JF - Photonics N2 - Many photochemical or photobiological applications require the use of high power ultraviolet light sources, such as high-pressure mercury arc lamps. In addition, many photo-induced chemical, biochemical and biological applications require either a combinatorial setting or a parallel assay of multiple samples under the same environmental conditions to ensure reproducibility. To achieve this, alternative, controllable light sources, such as ultraviolet light emitting diodes (UV LEDs) with high power and spatial control are required. Preferably, LEDs are arranged in a suitable standardized 96-well microtiter plate format. We designed such an array and established the methods required for heat management and enabling stable, controllable illumination over time. KW - optics KW - photonics KW - light KW - LED array KW - High-power LED KW - 365 nm KW - microtiter plate Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11307 SN - 2304-6732 VL - 6 IS - 1 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Yashchuk, Valeriy A1 - Bashmakova, Nataliya A1 - Gryn, Dmytro A1 - Hagemann, Tobias A1 - Naumenko, Antonina A1 - Kutsevol, Nataliya A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - A New Triple System DNA-Nanosilver-Berberine for Cancer Therapy JF - Applied Nanoscience N2 - The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA.In the current work we have investigated the effects of Berberine on the human T-cell leukemia cell line in vitro.Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time-and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction.Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers.The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanopoarticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment. KW - berberine KW - apoptosis KW - nanosilver KW - DNA delivery system Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10628 SN - 2190-5517 ER - TY - THES A1 - Schröter, Valentin T1 - Prototypischer Bau eines Tischroboters als Plattform für zukünftige Entwicklungs- und Forschungsprojekte im Bereich des Ambient Assisted Livings und als Alternative zum NAO-Roboter T1 - Prototypal construction of a table robot as a future development and research platform in the area of Ambient Assisted Living and as an alternative for the NAO-Robot N2 - In dieser Bachelorarbeit wurde ein prototypischer Tischroboter für den Einsatz im Bereich des Ambient Assisted Livings konzeptioniert. Innerhalb eines festgelegten Budgets wurden die technische Umsetzbarkeit und die Eignung des Systems als Alternative zum im Telematik-Studiengang der Technische Hochschule Wildau verwendeten NAO-Roboter untersucht. Dabei wurde bewusst auf die Möglichkeit einer humanoiden Fortbewegung verzichtet. Das entwickelte Konzept basiert auf den in dieser Arbeit festgelegten Anforderungen an einen Tischroboter. Es umfasst die benötigten Hardwarekomponenten, wobei als Grundlage des Systems ein Jetson Nano Einplatinencomputer von Nvidia verwendet wird. Weiterhin wurde eine Softwarearchitektur erstellt, welche Front-End, Back-End und angeschlossene Hardwarekomponenten miteinander verknüpft. Im Front-End wird das Framework Electron.js verwendet, im Back-End kommt das Robot Operating System (ROS) in Verbindung mit der Key-Value-Datenbank Redis zum Einsatz. Für die Steuerung weiterer angeschlossener Hardwarekomponenten und der Energieversorgung werden Arduino Nano Boards eingesetzt. Die konzipierte Architektur des Gesamtsystems ist auf eine funktionale Erweiterbarkeit ausgelegt, um für die Erforschung und Realisierung von vielfältigen Anwendungsfällen in Folgeprojekten geeignet zu sein. In einer anschließenden Testphase wurde ein Prototyp entwickelt, mit dessen Hilfe die konzipierte Systemarchitektur umgesetzt und erfolgreich getestet wurde. Die Ergebnisse dieser Bachelorarbeit können somit als prototypische Grundlage für eine Alternative zum NAO-Roboter, aber auch als technische Basis für die Entwicklung anderer Roboter verwendet werden. N2 - This thesis presents the concept for a prototypical table robot for use in the field of Ambient Assisted Living. The technical feasibility, within a given budget, and the suitability of this concept as an alternative to the robot NAO, which is used in the Telematics course of the Technical University of Applied Science Wildau, has been investigated. The aspect of humanoid locomotion was deliberately excluded from the scope of this thesis. The developed concept is based on a set of requirements for a table robot that are defined as part of this thesis. The concept includes the required hardware components and uses a Jetson Nano single-board computer from Nvidia as core of the system. In addition, a software architecture has been created in order to connect front-end, back-end and hardware components. The front-end uses the Electron.js framework, in the back-end the Robot Operating System (ROS) is used, as well as the key-value-database Redis. To control further hardware and the energy supplement, Arduino Nano Boards are used. The resulting architecture of the entire system is designed to be functionally extensible. Therefore, it is suitable for the investigation and realization of versatile use cases in future follow up projects. As part of the following testing phase, a prototype of the system has been developed. The prototype allowed successful realization and testing of the conceptualized architecture. Thus, the results of this thesis can serve both as a prototypical basis for an alternative to the robot NAO, and more broadly also as a technical foundation for the development of other robots. KW - ROS-E KW - Roboter KW - Tischroboter KW - AAL Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-15860 ER - TY - CHAP A1 - Chepurna, Oksana A1 - Grebinyk, Anna A1 - Petrushko, Yulia A1 - Prylutska, Svitlana A1 - Grebinyk, Sergii A1 - Yashchuk, Valeriy A1 - Matyshevska, Olga A1 - Ritter, Uwe A1 - Dandekar, Thomas A1 - Frohme, Marcus A1 - Qu, Junle A1 - Ohulchanskyy, Tymish Y. T1 - LED-based portable light source for photodynamic therapy N2 - Photodynamic therapy (PDT) employs light activation of tissue-localized photosensitizer in an oxygen-dependent process which initiates oxidative stress, inflammation, and cell death. Laser systems, which are mostly used in PDT as light sources can be costly and oversized. light-emitting diodes (LEDs) equipment has a high potential to simplify technical part of phototriggered therapies and to reduce its costs. We develop the LED-based system that includes the control and irradiation units. The system provides the same power density at any irradiation point. Among the advantages of the device is a possibility to change the irradiation area and tune the irradiation dose. PDT experiments with cancer cells in vitro treated with two different photosensitizers demonstrated a possibility to use the developed LED-based system as a low-cost light source in PDT. KW - light source KW - high power single chip LED KW - photodynamic therapy Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14778 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Buchelnikov, Anatoliy A1 - Tverdokhleb, Nina A1 - Grebinyk, Sergii A1 - Evstigneev, Maxim A1 - Matyshevska, Olga A1 - Cherepanov, Vsevolod A1 - Prylutskyy, Yuriy A1 - Yashchuk, Valeriy A1 - Naumovets, Anton A1 - Ritter, Uwe A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells JF - Pharmaceutics N2 - A herbal alkaloid Berberine (Ber), used for centuries in Ayurvedic, Chinese, Middle-Eastern, and native American folk medicines, is nowadays proved to function as a safe anticancer agent. Yet, its poor water solubility, stability, and bioavailability hinder clinical application. In this study, we have explored a nanosized carbon nanoparticle—C60 fullerene (C60)—for optimized Ber delivery into leukemic cells. Water dispersions of noncovalent C60-Ber nanocomplexes in the 1:2, 1:1, and 2:1 molar ratios were prepared. UV–Vis spectroscopy, dynamic light scattering (DLS), and atomic force microscopy (AFM) evidenced a complexation of the Ber cation with the negatively charged C60 molecule. The computer simulation showed that π-stacking dominates in Ber and C60 binding in an aqueous solution. Complexation with C60 was found to promote Ber intracellular uptake. By increasing C60 concentration, the C60-Ber nanocomplexes exhibited higher antiproliferative potential towards CCRF-CEM cells, in accordance with the following order: free Ber < 1:2 < 1:1 < 2:1 (the most toxic). The activation of caspase 3/7 and accumulation in the sub-G1 phase of CCRF-CEM cells treated with C60-Ber nanocomplexes evidenced apoptosis induction. Thus, this study indicates that the fast and easy noncovalent complexation of alkaloid Ber with C60 improved its in vitro efficiency against cancer cells. KW - C60 fullerene KW - berberine KW - noncovalent nanocomplex KW - UV–Vis KW - DLS and AFM measurements KW - drug release KW - leukemic cell KW - uptake KW - cytotoxicity KW - apoptosis Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12932 SN - 1999-4923 VL - 11 IS - 11 PB - MDPI ER - TY - JOUR A1 - Grebinyk, Anna A1 - Yashchuk, Valeriy A1 - Bashmakova, Nataliya A1 - Gryn, Dmytro A1 - Hagemann, Tobias A1 - Naumenko, Antonina A1 - Kutsevol, Nataliya A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - A new triple system DNA-Nanosilver-Berberine for cancer therapy JF - Applied Nanoscience N2 - The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA. In the current work, we have investigated the effects of Berberine on the human T cell leukemia cell line in vitro. Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time- and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction. Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers. The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanoparticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16105 SN - 2190-5517 VL - 9 SP - 945 EP - 956 PB - Springer ER - TY - CHAP A1 - Pohle, Ulrike A1 - Baumann, Elisabeth A1 - Pulwer, Silvio A1 - Villringer, Claus A1 - Zhang, Edward A1 - Gerhardt, Holger A1 - Laufer, Jan T1 - Development of a backward-mode photoacoustic microscope using a Fabry-Pérot sensor N2 - Optical-resolution photoacoustic microscopy (PAM) has been shown to enable the acquisition of high resolution (μm) functional and anatomical images. For backward-mode operation, conventional piezoelectric ultrasound transducers need to be placed far away from the signal source due to their opacity and size. This can result in reduced acoustic sensitivity. Planar Fabry-Perot polymer film interferometer (FPI) sensors have the potential to overcome this limitation since they are transparent to the excitation wavelength, can be placed immediately adjacent to the signal source for high acoustic sensitivity, and offer a broadband frequency response (0 –50 MHz). In this study, we present a high frame rate, backward-mode OR-PAM system based on a planar FPI ultrasound sensor. A ns-pulsed laser provides excitation pulses (<200 nJ, maximum pulse repetition frequency = 200 kHz, 532 nm) to generate photoacoustic waves that are detected using a planar FPI sensor interrogated at 765-781 nm. For backwardmode operation and highest acoustic sensitivity, the excitation and interrogation beams are coaxially aligned and rasterscanned. The optical transfer function of the sensor, the spatial resolution and the detection sensitivity were determined to characterise the set-up. Images of a leaf phantom and first in vivo images of zebrafish larvae were acquired. This approach will enable fast 3D OR-PAM with high resolution and high sensitivity for functional and molecular imaging applications. FPI-based ultrasound detection also has the potential to enable dual-mode optical- and acousticresolution PAM and the integration of photoacoustic imaging with purely optical modalities such as multi-photon microscopy. KW - optical-resolution photoacoustic microscopy KW - Fabry-Perot ultrasound sensor KW - in vivo imaging Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-15349 SP - 594 EP - 598 PB - SPIE ER - TY - JOUR A1 - Marehn, David Thomas A1 - Wilhelm, Detlef A1 - Pospisil, Heike A1 - Pizzoferrato, Roberto T1 - Double entry method for the verification of data a chromatography data system receives JF - Journal of Sensors and Sensor Systems N2 - The importance of software validation increases since the need for high usability and suitability of software applications grows. In order to reduce costs and manage risk factors, more and more recommendations and rules have been established. In the field of pharmacy the vendors of so-called chromatography data systems (CDSs) had to implement the guidelines of the Code of Federal Regulations Title 21 (CFR 21) during the last few years in order to fulfill the increasing requirements. The CFR 21 part 11 deals with electronic records and signatures. This part is binding for each company in the regulated environment that wishes to create, edit and sign electronic information instead of printing them on paper. Subsection CFR 21 part 11.10(h) explains how to perform an input check for manual user entries as well as for data that will be collected from an external device. In this article we present an approach performing the double entry method on data provided by the hardware instrument in order to investigate possible influences on the raw data by the handling CDS. A software tool has been written which allows us to communicate with a high-performance liquid chromatography (HPLC) detector and acquire data from it. The communication is completely independent of a CDS which is started separately and connected to the same system. Using this configuration we made a parallel data acquisition of two instances at the same time possible. Two CDSs have been tested and for at least one of them it has been shown that a comparison of the acquired data can be done as with the double entry method for the data verification. For the second CDS we checked whether it would be applicable after a few modifications. The given approach could be either used for a live data verification of produced raw data or as a single test during a software operational qualification to verify the data acquisition functionality of the software. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12349 SN - 2194-878X VL - 8 SP - 207 EP - 214 ER - TY - GEN A1 - Schäfer, Marco A1 - Jetschni, Christian A1 - Wang, Chong A1 - Pospisil, Heike T1 - Optimierung des Rekonstruktions-Tools TSNI für genregulatorische Netzwerke mittels Parallelisierung und ein Vergleich mit Time Delay ARACNE T2 - Wissenschaftliche Beiträge 2019 N2 - Gegenstand dieser Veröfffentlichung ist eine Reimplementierung des TSNI (Time Series Network Identification)-Tools zur Rekonstruktion von genregulatorischen Netzwerken aus Zeitserien von Gen-Expressionsprofilen. Das auf gewöhnlichen Differentialgleichungen basierende Tool wurde algorithmisch verbessert und an die Verarbeitung großer Datensätze angepasst. Durch Ausschöpfung des Potentials moderner Multi-CPU und GPU-Architekturen wurden die erforderlichen Berechnungen erheblich beschleunigt. Bei der Überführung des mit Matlab implementierten Tools in die Programmiersprache C wurde zudem die ­Bedienbarkeit verbessert. Durch Einsatz der GPU-beschleunigten Mathematik-Bibliothek MAGMA konnten unter Verwendung eines normalen Desktop-Rechners Leistungssteigerungen um das 18,7-fache bei 11.000 Genen verzeichnet werden. Darüber hinaus rekonstruierte das verbesserte TSNI weitaus mehr biologisch richtige Beziehungen als vergleichbare Anwendungen. N2 - The purpose of this work was to optimize a tool for analyzing biological high-throughput data and to generate gene regulatory networks, which are based on ordinary differential equations. For this task the Time Series Network Indentification (TSNI) - tool has been selected. The TSNI-algorithm has been optimized and transferred into the programming language C. Computationally intensive sections were parallelized using modern multi-core CPU and GPU architectures as well as functions from the MAGMA-library for the solution and parallelization of more complex mathematical problems. The performance of the algorithm was significantly increased (e.g. the calculation of a network of more than 11.000 genes was 18.7-fold faster with the improved tool compared to the original algorithm). So, large regulatory networks can now be ­analysed in a moderate time. Additionally, more true positive network connections were predicted using the adjusted TSNI - tool. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10767 SN - 0949-8214 VL - 23 SP - 7 EP - 10 ER - TY - JOUR A1 - Kagel, Heike A1 - Frohme, Marcus A1 - Glökler, Jörn T1 - Photoacids in biochemical applications JF - Journal of Cellular Biotechnology N2 - BACKGROUND: After excitation with light photoacids can change the pH in a solution by release of a proton. They have been used mostly for excited state proton transfer studies. In this review the general functionality and mechanisms and the subdivision of photoacids is explained. STATE OF THE ART: Different uses of photoacids are described, covering a wide range of various biochemical topics, focusing on biochemical applications. Examples for the introduced subdivisions are covered. CONCLUSIONS AND OUTLOOK: The areas in which photoacids can be employed are diverse. Photoacids have a promising future in biotechnology and biochemistry and should be considered for upcoming applications, especially in non-invasive control of biochemical reactions. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16123 SN - 2352-3697 VL - 4 IS - 1-2 SP - 23 EP - 30 PB - IOS Press ER - TY - CHAP A1 - Pulwer, Silvio A1 - Fiebelkorn, Richard A1 - Zesch, Christoph A1 - Steglich, Patrick A1 - Villringer, Claus A1 - Villasmunta, Francesco A1 - Gedat, Egbert A1 - Handrich, Jan A1 - Schrader, Sigurd A1 - Vandenhouten, Ralf T1 - Endoscopic orientation by multimodal data fusion T2 - Proc. SPIE 10931, MOEMS and Miniaturized Systems XVIII N2 - To improve the feasibility of endoscopic inspection processes we developed a system that provides online information about position, orientation and viewing direction of endoscopes, to support the analysis of endoscopic images and to ease the operational handling of the equipment. The setup is based on an industrial endoscope consisting of a camera, various MEMS and multimodal data fusion. The software contains algorithms for feature and geometric structure recognition as well as Kalman filters. To track the distal end of the endoscope and to generate 3D point cloud data in real time the optical and photometrical characteristics of the system are registered and the movement of the endoscope is reconstructed by using image processing techniques. KW - endoscope KW - orientation KW - Kalman filter KW - visual odometry KW - mems KW - gyroscope KW - accelerometer Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11420 SN - 1996-756X ER - TY - CHAP A1 - Zesch, Christoph A1 - Schrader, Sigurd A1 - Prosposito, Paolo A1 - Lux, Oliver A1 - Eichler, Hans J. T1 - Raman lasers for trace gas detection N2 - The emission of commercial solid-state lasers is shifted by Raman lasers to selected wavelengths suitable for trace gas detection. High power Raman lasers based on Ba(NO3)2, diamond and silicon detect CO2, O3 and H2O. Raman lasers base on the physical effect of stimulated Raman scattering. Two new Raman crystals (Spodumene and LuAlO3) are investigated and their SRS-spectra is shown. KW - stimulated Raman scattering KW - Raman laser KW - Barium nitrate KW - Nd:YAG laser KW - trace gas detection Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-15351 SP - 114 EP - 119 PB - SPIE ER - TY - JOUR A1 - Bauer, Joachim A1 - Fursenko, Oksana A1 - Marschmeyer, Steffen A1 - Heinrich, Friedhelm A1 - Villasmunta, Francesco A1 - Villringer, Claus A1 - Zesch, Christoph A1 - Schrader, Sigurd T1 - Spectroscopic reflectometry for characterization of Through Silicon Via profile of Bosch etching process JF - Journal of Vacuum Science & Technology B N2 - Through Silicon Via (TSV) technology is a key in 3D integration of circuits by the creation of interconnects using vias, which go through the full silicon wafer. Typically, a highly-selective Bosch Si etch process is used. It is characterized by a high etch rate at a high aspect ratio, whereby scallops on the sidewalls are generated. In this work, square via arrays with dimensions from 3 to 50 μm and up to 300 μm depth were fabricated and analyzed by spectroscopic reflectometry. The reflectometric data are compared to simulations by a novel theoretical approach. In order to simulate the reflectance spectra of TSV arrays, a combination of 2D and 3D rigorous coupled wave analysis was applied. Besides the via depth, the sidewall angle and the corner radius of the bottom profile were considered in the model. The general requirements on spectral resolution in TSV metrology are discussed. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-16093 VL - 37 IS - 6 PB - American Vacuum Society (AVS) ER - TY - GEN A1 - Hartmann, Lutz A1 - Tatai, Tiberiu-Mihai A1 - Kaiser, Mario A1 - Dreyer, Christian T1 - Erwärmung von Materialien und Werkstoffen durch Anwendung von Mikrowellentechnologien T2 - Wissenschaftliche Beiträge 2019 N2 - Die Erwärmung unterschiedlicher Materialien im ­Kontext verschiedener Anwendungen kann anhand der Bestrahlung mit Mikrowellen in geeignet konstruierten Öfen erfolgen. Der vorliegende Beitrag beginnt mit einer Vorstellung der mikroskopischen Mechanismen der Erwärmung von Materie mittels Mikrowellen sowie einem Abriss ihrer theoretischen Beschreibung. Im Folgenden werden die breitbandige dielektrische Spektroskopie und die Simulation elektromagnetischer Felder als Hilfsmittel eingeführt, die Effizienz des mikrowellenbasierten Heizens für bestimmte Materialien vorab einschätzen zu können. Nach der Vorstellung zweier unterschiedlicher Mikrowellenanlagen, namentlich eines Kammer- und eines Durchlaufmikrowellenofens, wird die thermische Behandlung unterschiedlicher Materialien mittels Mikrowellen an drei Beispielen illustriert. N2 - The heating of various materials in the context of different applications can be achieved by irradiation with microwaves in properly constructed microwave ovens. This article starts with an introduction to the microscopic mechanisms of heating of matter followed by a survey of their theoretical description. Both, broadband dielectric spectroscopy and the simulation of electromagnetic fields will be presented as convenient tools to estimate the efficiency of microwave-based heating of certain materials prior to any experiment. In addition to an introduction of two different microwave ovens, namely a chamber oven and a throughput oven, the thermal treatment of different materials is illustrated by three examples. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10812 SN - 0949-8214 VL - 23 SP - 39 EP - 48 ER - TY - GEN A1 - Hotes, Andreas G. A1 - Schwahn, Klaus-Jürgen A1 - Fabienke, Henrike T1 - Betrachtung von neuartigen Antriebs- und Kraftstoffkonzepten mit dem Ziel der Konzeption ­einer Hybridtankstelle für den Flugplatz Schönhagen für die Versorgung von Luftfahrzeugen mit nachhaltigen Kraftstoffen T2 - Wissenschaftliche Beiträge 2019 N2 - Dieser wissenschaftliche Beitrag befasst sich zunächst mit der Betrachtung von neuartigen und nachhaltigen Antriebs- und Kraftstoffkonzepten für den Einsatz in der Luftfahrt, die zum Erreichen der hochgesteckten Klimaschutzziele auf nationaler, europäischer und internationaler Ebene beitragen sollen. Um die Energieversorgung dieser Luftfahrzeuge gewährleisten zu können, wurde ein Tankstellensystem beispielhaft für den Flugplatz Schönhagen konzipiert, welches Strom, Wasserstoff und Power-to-Liquid-Kraftstoff für Elektro-, Wasserstoff- und Luftfahrzeuge mit konventionellem Verbrennungsmotor sowie für Hybridflugzeuge zur Verfügung stellen soll. Ziel dieses Konzeptes ist die komplette Eigenproduktion der Kraftstoffe auf dem Flugplatz. Hierzu wurden rechtliche, wirtschaftliche und vor allem technische Voraussetzungen untersucht und ausgewertet sowie Berechnungen bezüglich der benötigten Kraftstoff- und Stromkapazitäten durchgeführt. N2 - This paper will initially focus on novel and sustainable propulsion and fuel concepts for use in aviation, which should contribute to the achievement of ambitious climate protection goals at national, European an international level. To ensure the energy supply of these aircrafts, a filling station system was designed as an example for the Schönhagen airfield, which provides electricity, hydrogen and power-to-liquid-fuel for electric and hydrogen driven aircrafts, aircrafts with conventional combustion engines as well as for hybrid aircrafts. The aim of this concept is the complete in-house production of fuels on the airfield. For this purpose, legal, economic, and above all technical requirements, were examined and evaluated and calculations were carried out regarding the required fuel and power capacities. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11939 SN - 0949-8214 VL - 23 SP - 49 EP - 55 ER - TY - GEN A1 - Gerhard, Christoph A1 - Letien, Emilie A1 - Cressent, Thomas A1 - Hofmann, Mandy T1 - Impact of the plasma power on plasma-induced increase in absorption of fused silica T2 - Wissenschaftliche Beiträge 2019 N2 - In the last decades, laser machining of fused silica has gained in importance where the main challenge is to overcome the high transmission of this medium in order to achieve surface absorption. One approach is plasma treatment using hydrogenous process gases. In doing so, a near-surface glass layer is chemically modified by a removal of oxygen and an implantation of hydrogen, resulting in the formation of optically active defects and an increase in absorption. Against this background, the impact of the plasma power on plasma-induced increase in absorption of fused silica was investigated in the present work. Here, the highest efficiency of the plasma treatment process was found at the lowest applied plasma power whereas for higher plasma powers, a notably lower increase in absorption was observed. This effect is attributed to the decomposition of plasma species required for initiating the formation of optically active defects within the glass. Such decomposition increases with rising power due to the corresponding increase in electric field strength within the plasma. This finding is of substantial interest for an extensive understanding of plasma-glass interactions. N2 - Die Laserbearbeitung von Quarzglas hat in den letzten Jahrzehnten an Bedeutung gewonnen. Dabei besteht die Herausforderung darin, die hohe Transmission dieses Mediums zu überwinden. Hierzu stellt eine Plasmabehandlung mittels wasserstoffhaltigen Prozessgasen einen Ansatz dar. Dabei wird eine oberflächennahe Glasschicht durch Entfernung von Sauerstoff und Implantation von Wasserstoff chemisch modifiziert, woraus die Ausbildung optisch aktiver Defekte und ein Anstieg der Absorption resultieren. Vor diesem Hintergrund wurde in dieser Arbeit der Einfluss der Plasmaleistung auf die plasmainduzierte Absorptionssteigerung untersucht. Die höchste Effizienz der Plasmabehandlung wurde bei der geringsten applizierten Leistung erzielt, wohingegen bei höheren Plasmaleistungen nennenswert geringere Absorptionssteigerungen beobachtet wurden. Dieser Effekt wird auf den Zerfall von Plasmaspezies, die für eine Ausbildung optisch aktiver Defekte benötigt werden, zurückgeführt, welcher mit ansteigender Leistung und dem damit einhergehenden Anstieg der elektrischen Feldstärke im Plasma anwächst. Diese Erkenntnis ist von hohem Interesse für ein umfassendes Verständnis von Plasma-Glas-Wechselwirkungen. N2 - Ces dernières décennies, les applications laser en usine composées de silice fondue ont gagné en importance, l'enjeu consistant à surmonter le problème de la transmission élevée de ce milieu dans le but d’obtenir l’absorption de surface. Une façon d’aborder le problème est d’appliquer le traitement au plasma en utilisant des procédés au gaz de dihydrogène. En agissant ainsi, une couche de verre proche de la surface est modifiée chimiquement par l’ablation d’oxygène et l’implantation d’hydrogène, ce qui provoque la formation de défauts optiquement actifs et l’augmentation de l’absorption. Dans ce contexte, l’impact de la puissance du plasma sur l’augmentation de l’absorption de silice fondue induite par le plasma a été étudié dans cet article. Ici, la meilleure efficacité du traitement au plasma a été constatée à la puissance de plasma la plus faible tandis que pour de plus fortes puissances, une augmentation bien plus faible en absorption a été observée. Cet effet est attribué à la décomposition des espèces de plasma requises dans la formation des défauts optiquement actifs à l’intérieur du verre. Une telle décomposition augmente avec la montée de puissance due à l'augmentation de force du champ électrique dans le plasma. Cette découverte est d’un intérêt considérable pour une compréhension plus poussée des interactions plasma-verre. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10802 SN - 0949-8214 VL - 23 SP - 33 EP - 37 ER - TY - GEN A1 - Handrich, Jan A1 - Vandenhouten, Ralf T1 - Erweiterung eines landmarkenbasierten Innenraumortungsverfahrens für Mobilgeräte mit ARCore T2 - Wissenschaftliche Beiträge 2019 N2 - Die Lokalisierung in Innenräumen, die sich nicht auf Satellitennavigation wie GPS verlassen kann, ist nach wie vor eine Herausforderung. Viele Methoden zur Lokalisierung in Innenräumen beruhen auf Funksignalen oder anderen Sendern (z.B. Infrarot), die eine kostenintensive Infrastruktur erfordern, die in einem Gebäude installiert werden muss. Außerdem sind diese Techniken ungenau, wenn Reflexionen der Signale in einem Gebäude auftreten. Die Forschungsgruppe Telematik der TH Wildau hat für diese Problemstellung ein optisches Verfahren zur Innenraumlokalisierung entwickelt, das die Kamera eines mobilen Geräts verwendet. Dieser Ansatz erfordert nur die Verfügbarkeit von mehreren identifizierbaren Landmarken, die kostengünstig in einem Gebäude installiert werden können. In diesem Beitrag soll eine Möglichkeit vorgestellt werden, dieses landmarkenbasierte Verfahren mit der Augmented Reality-Bibliothek ARCore von Google zu erweitern, um eine kontinuierliche Positionsermittlung zu ermöglichen, auch wenn vorübergehend keine Landmarken durch die Gerätekamera erfasst ­werden können. N2 - Indoor localization, which cannot rely on satellite navigation such as GPS, remains a challenge. Many indoor localization methods depend on radio signals or other transmitters (e.g. infrared) that require a costly infrastructure to be installed in a building. In addition, these techniques are inaccurate when reflections of the signals occur in a building. The telematics research group at TH Wildau has developed an optical method for indoor localization for this problem that uses the camera of a mobile device. This approach only requires the availability of several identifiable landmarks that can be installed cost-effectively in a building. In this article, a way is presented to extend this landmark based method with Google's Augmented Reality library ARCore to enable continuous position tracking even if no landmarks can be captured by the device ­camera temporarily. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10790 SN - 0949-8214 VL - 23 SP - 27 EP - 32 ER - TY - GEN A1 - Schwahn, Cheryl Nicoletta A1 - Angermann, Sven A1 - Rüther-Kindel, Wolfgang T1 - Untersuchungen zu Mensch-Maschine-Schnittstellen eines Pilotenassistenzsystems zur Begegnung potentieller Gefahrensituationen der Allgemeinen Luftfahrt T2 - Wissenschaftliche Beiträge 2019 N2 - Privatpiloten, die sich in der Allgemeinen Luftfahrt bewegen, sind häufig anderen Gefahren ausgesetzt, als Piloten großer Verkehrsflugzeuge. Gefahrensituationen bringen Piloten schnell an Wissenslücken und Kapazitätsgrenzen. Für Privatpiloten stellt dabei der Kontrollverlust im Flug die häufigste Unfallursache dar. Das Forschungsvorhaben Virtual Instructor for General Aviation (VIGA) des Fachgebiets Luftfahrttechnik leistet einen wesentlichen Beitrag zur Vermeidung dieser Unfälle. Ziel des Vorhabens ist es, ein Assistenzsystem zu entwickeln, das Privatpiloten frühzeitig mit Handlungsempfehlungen unterstützt, sobald diese in eine unkontrollierte Fluglage gelangen. Dieser wissenschaftliche Beitrag stellt die Grundlagen des Forschungsvorhabens VIGA dar und beschreibt eine Konzepterstellung zur Mensch-Maschine-Schnittstelle von Assistenzsystemen der zivilen Luftfahrt unter Betrachtung der psychologischen Belastung in Notsituationen. Belastungssituationen beeinträchtigen sowohl die Wahrnehmung der Piloten, beispielsweise durch selektive Taubheit oder optische Täuschungen als auch die variierende Reaktionszeit des Piloten auf verschiedene Reize. Ziel ist es, eine geeignete Mensch-Maschine-Schnittstelle zu entwickeln, die jederzeit die Aufmerksamkeit des Piloten erlangt und Handlungsempfehlungen zielgerecht an diesen ausgibt. Erkenntnisse aus diesem Beitrag finden direkte Anwendung im Vorhaben VIGA. N2 - Private pilots in the field of private transportation have a higher risk to experience an incident than pilots of air carriers. Incidents become hazardous as soon as pilots reach the boundaries of their knowledge and capacities. For private pilots Loss of Control Inflight is the most common cause for an incident. The research project Virtual Instructor for General Aviation (VIGA) is countering with this problem by creating an assistance system which transmits recommended actions in hazardous situations before a Loss of Control Inflight occurs. This paper summarizes the basic concept of the research project and comprises the concept of human-machine interfaces used in assistance systems in the field of civil aviation. During situations with a high workload the perception is changing. For example, Pilots experience selective auditory attention, visual illusions and a changing response time referring to the attraction. Therefore, it is the aim to create an appropriate human-machine interface which targets the attention and transmits recommended actions to the private pilot. The awareness is used as the foundation of the project VIGA. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11940 SN - 0949-8214 VL - 23 SP - 57 EP - 62 ER - TY - GEN A1 - Oeckel, Konrad A1 - Heimann, Jan A1 - Angermann, Sven A1 - Kerscher, Michael A1 - Frahm, Andreas A1 - Heilmann, Gunnar A1 - Rüther-Kindel, Wolfgang T1 - Vergleichende akustische Untersuchung von Drohnen-Propellern T2 - Wissenschaftliche Beiträge 2019 N2 - Die Reduzierung von Fluglärm ist eine der wesentlichen Herausforderungen der Luftfahrtindustrie. Dies betrifft ebenso unbemannte Luftfahrtsysteme, die zukünftig eine signifikante Rolle im alltäglichen Leben spielen werden. In diesem Zusammenhang wurde im Fachgebiet Luftfahrttechnik der TH Wildau das energetische Potential von Winglets an Propellern („Proplets“) untersucht, deren Einsatz sowohl eine Verbesserung der aerodynamischen Effizienz als auch eine Minderung der Geräuschemission versprachen. Daher musste der akustische Einfluss dieser Proplets präzise bestimmt werden. In diesem Beitrag wird erstmals die Geräuschemission eines mit Proplets ausgerüsteten Propellers mit einem Referenzpropeller verglichen. Beide Prüfstücke sind, abgesehen von den Blattspitzen, identisch. Neben normgerechten, herkömmlichen Messungen der Schallleistungspegel sowie psychoakustischer Eigenschaften werden beide Prüfstücke mit einem Mikrofon-Array („Akustische Kamera“) untersucht, um die Lärmquellen entlang der Propellerblätter zu visualisieren. Hierfür wird erstmals ein virtuell mitrotierendes Array angewendet, um die aero-akustischen Quellen am stehenden Blatt zu identifizieren. N2 - The reduction of aircraft noise is one of the major challenges of the aviation industry. This also concerns unmanned aerial vehicles (UAVs), since they will play an increasingly important role in everyday life. In the context of the presented project the energetic potentials of winglets at propeller tips ("proplets") were investigated in order to increase the aerodynamic efficiency and decrease the noise emission of propellers. Therefore it was necessary to determine the acoustic effects of the attached proplets. This paper compares the noise emission of a reference propeller with a proplet-equipped propeller for the first time. Except for the tips, they were identically designed. The acoustic power level of both propellers was determined and the results are assessed psycho-acoustically. Additionally an examination of the test pieces with a spaced microphone array ("Acoustic Camera") was conducted to visualize the localization of sound sources on the propellers. A rotational beamforming filter was applied to precisely identify the positions of the rotating aero-acoustic sources. KW - rotational beamforming KW - Fluglärm KW - Propellerlärm KW - Winglet KW - Proplet KW - Akustische Kamera KW - Schallquellenlokalisierung Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11957 SN - 0949-8214 VL - 23 SP - 63 EP - 69 ER - TY - GEN A1 - Riedel, Marc A1 - Schäfer, Daniel A1 - Parak, Wolfgang J. A1 - Ruff, Adrian A1 - Schuhmann, Wolfgang A1 - Lisdat, Fred T1 - Quantum Dot-modifizierte TiO2-Strukturen für die Licht-gesteuerte Bioelektrokatalyse T2 - Wissenschaftliche Beiträge 2019 N2 - Die funktionale Kopplung von photoaktiven Nanostrukturen mit Enzymen stellt eine neue Strategie zum Aufbau lichtgesteuerter biohybrider Systeme dar. Hier sind Untersuchungen zusammengefasst, welche die effiziente Kontaktierung der FAD-abhängigen Glukosedehydrogenase (FAD-GDH) mit Hilfe eines Osmium-Redoxpolymers (P Os ) an PbS-Quantum Dots (PbS QDs) zeigen, welche direkt auf dreidimensionalen TiO 2 -Elektrodenstrukturen synthetisiert wurden. Diese biohybriden Strukturen erlauben die Licht-induzierte Oxidation von Glukose. Dazu wird zunächst ein Verfahren vorgestellt, bei welchem durch den Aufbau invers-opaler TiO 2 (IO-TiO 2 ) Strukturen hohe Bindungskapazitäten für die Integration von QDs, Redoxpolymer und Enzym erreicht werden. In Folge wird gezeigt wie elektrochemische Signalketten durch Licht gesteuert werden können, indem Ladungsträger in den QDs unter Beleuchtung erzeugt werden. Diese Aktivierung ermöglicht dann die Ausbildung einer Elektrontransferkaskade vom Enzym über das Redoxpolymer zu den QDs und final zur IO-TiO 2 -Elektrode. Die resultierenden anodischen Photoströme können durch das Potential, die Lichtintensität und die Glukosekonzentration moduliert werden. So können in Anwesenheit von Glukose Photoströme von bis zu 207 μA/cm2 und erste Oxidationssignale bereits bei einem Potential von -540 mV vs Ag/AgCl, 1 M KCl erhalten werden. Dies entspricht einem Potentialgewinn von über 500 mV im Vergleich zu nicht lichtsensitiven Elektroden. Das vorgestellte biohybride System kombiniert Vorteile einer großen Oberfläche (durch IO-TiO 2 -Struktur), die effiziente Ladungsträgergenerierung und -trennung an der QD/TiO 2 -Schnittstelle sowie die effiziente Kontaktierung von FAD-GDH mit den QDs mit Hilfe eines Redoxpolymers. Die Ergebnisse verdeutlichen das Potential dieser leistungsfähigen Photobioanode für die Sensorik und die Erzeugung von Energie aus Licht und Glukose. N2 - The combination of photoactive nanostructures with enzymes represents a new strategy for the construction of light-directed biohybrid systems. The study demonstrates the efficient linkage of FAD-dependent glucose dehydrogenase (FAD-GDH) to PbS quantum dot (QD)-sensitized inverse opal TiO 2 (IO-TiO 2 ) electrodes using an osmium redox polymer (P Os ). How these biohybrid structures can oxidize glucose in a light-directed fashion is highlighted in detail. For this purpose, firstly a method for the construction of IO-TiO 2 electrodes is presented, which gives rise to high binding capacities for the integration of QDs, redox polymer and enzyme. It can be shown how electrochemical signal chains can be controlled with light, resulting in a generation of charge carriers within the QDs. The activation of the electron transfer cascade then allows for an electron transfer from the enzyme via the redox polymer to the QDs and finally to the IO-TiO 2 electrode. The resulting anodic photocurrents can be modulated by potential, light intensity and glucose concentration. In the presence of glucose, photocurrents of up to 207 μA/cm 2 can be generated and first electron withdrawal from the biocatalytic sugar oxidation already proceeds at a potential of -540 mV vs Ag/AgCl (1 M KCl). This corresponds to a potential gain of over 500 mV compared to light-insensitive electrodes. The proposed biohybrid system combines the advantages of a large surface area (IO-TiO 2 ), efficient charge carrier generation (PbS QDs) and separation at the TiO 2 /QDs interface and the efficient linkage of FAD-GDH to the QDs using a redox polymer. This illustrates the potential of this powerful photobioanode for sensing and power supply. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10773 SN - 0949-8214 VL - 23 SP - 11 EP - 17 ER - TY - CHAP A1 - Alarcón, Daniel A1 - Schneider, Sarah A1 - Kamenzky, Robert A1 - Blaschke, Peter T1 - Across the Picket Fence: Influence of Sampling Frequency in Automatic Impact Modal Testing T2 - Special Topics in Structural Dynamics, Volume 5 : Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018 N2 - Automatic impact modal testing is a technique gaining momentum in recent years thanks to the popularization of Scanning Laser Doppler Vibrometry. These systems allow automatizing the output measurement of thousands of degrees of freedom in a short time. The use of automatic impact modal hammers allows automatizing the excitation input and broadband excitation without loading a structure with an extra mass or other drawbacks. However, the impact force repeatability is a prominent concern among test engineers, especially those who work with materials with non-proportional force/response ratios. Assessing the impact force repeatability of a given automatic modal hammer or test rig is necessary in order to ensure the right response level is measured impact after impact. The assessment procedure can be misleading if not done right. Studying the automatic modal hammer repeatability under typical modal test conditions invariably leads to impact signals strongly distorted by the so called picket fence effect. This results in impacts sampled by only 3–4 data points; insufficient to accurately describe the actual impact force signals and the short contact times between hammer tip and structure. In the reality, the impacts are of larger magnitudes and shorter contact times than what is shown by the analyzer in typical test conditions. This work studies the influence of the sampling frequency and the test structures used on the repeatability assessment of automatic impact modal hammers. Impact force signals are acquired in this work with enough resolution to eliminate the picket fence effect and truly evaluate how repeatable and reproducible automatic impacts are. The practicality of the procedure, which involves very large datasets and long testing times, is discussed. Guidelines are offered at the end of the paper for a successful repeatability and reproducibility assessment of automatic impact modal hammers. KW - experimental modal analysis KW - sampling KW - automatic modal hammer KW - impact modal testing KW - validation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21515 PB - River Publishers ER - TY - CHAP A1 - Schneider, Sarah A1 - Mallareddy, Tarun Teja A1 - Alarcón, Daniel A1 - Kamenzky, Robert A1 - Blaschke, Peter T1 - Experimental Modal Analysis of Structures with Conventional Versus Contact-Free Suspension T2 - Special Topics in Structural Dynamics, Volume 5 : Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018 N2 - The existence of free boundary conditions is frequently assumed for Experimental Modal Analysis (EMA) of a structure. However, free-free conditions can only be approximated because the structure must be supported in some manner. Therefore, comparing simulated data with experimental data can be deceiving, because these suspensions falsify modal parameters especially structural damping and stiffness. The current scenario of structural analysis is more towards focusing on modal updating or correlation, rather than the simulation results (FE) or the experimental results. So it is imperative to bridge the gap between FE and EMA, by carefully studying various parameters. To overcome these drawbacks, levitation is suggested as a truly free-free suspension method. The levitation method was developed to allow a non-destructive, adaptable, and completely contactless approach for material testing: the structure under test is suspended on a thin film of pressurized air providing an aerodynamic bearing, levitating the specimen. Two suspension devices were constructed. Pressurized air is circulated into a casing with a single outlet (“air cushion”) or a fine grid of outlets (“air bed”). A study was performed to investigate the influence of the support conditions on the modal parameters eigenfrequency and damping. Tested specimens were a brass plate, a stainless steel plate and two composite material probes. The tested suspension methods were (a) foam mat, (b) air cushion and (c) air bed. Modal tests were performed using a Scanning Laser Doppler Vibrometer (SLDV) and an automatic modal hammer for excitation. Evaluations of the measurements were performed manually. The results showed that the detected eigenfrequencies of the metallic specimen have a variation below ±0.3% for the tested suspension methods. This variation is 10 times higher for the composite plates and lies between ±3%. The damping ratios of the levitation suspensions show the different material behavior of metallic and composite specimen: damping ratios of metallic specimen lie between 0.05–0.5% whereas damping ratios of composite plates are ten times higher and lie between 0.3% and 3%. The damping ratios measured with the air cushion are smaller than the damping ratios for the air bed supporting the hypothesis that a laminar air film under the specimen leads to less additional damping. The study shows that EMA can be performed on metallic and composite specimens using contact-less suspension methods. Especially for light-weight material specimens where EMA cannot be performed or where the results are not reliable, the contact-less suspension (levitation method) can be used. KW - modal analysis KW - levitation KW - free-free suspension KW - composite material KW - automatic modal hammer Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21533 PB - River Publishers ER - TY - CHAP A1 - Mallareddy, Tarun Teja A1 - Alarcón, Daniel A1 - Schneider, Sarah A1 - Blaschke, Peter T1 - The Influence of Geometrical Correlation in Modal Validation Using Automated 3D Metrology T2 - Rotating Machinery, Vibro-Acoustics & Laser Vibrometry, Volume 7 : Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018 N2 - Structural analysis is a major part of all manufacturing and testing industries. The need for high level accuracy of the results in the testing field has increased progressively, resulting in development of advanced state of art techniques. In order to acquire the vibrational characteristics of a structure, a detailed Finite Element Analysis (FEA) modelling is performed. Also, Experimental Modal Analysis (EMA) is conducted to extract the dynamic characteristics of a structure. The results obtained from both the processes are correlated for validation purposes. Based on the correlation (good or bad) the structural analysis is validated. In most cases the correlation is not satisfactory; it is mainly because of the boundary conditions that differ in FE and EMA. This research study explains in detail how important the boundary conditions are for modal validation. But the most imperative part, as the first step of correlation, is the geometry analysis. If the geometrical correlation is not accurate, the later part of correlation will turn out to be an assumption based on inaccuracies. Assumption of a geometrical correlation, without being sure of the differences, will lead to inaccurate results for validation. A reference plate is tested and simulated by using EMA and FEA techniques respectively. EMA is conducted by using a 3D SLDV for measuring the output response and the input force of excitation is induced by a Scalable Automatic Modal hammer (SAM). This plate is then scanned using ATOS Triple Scan II GOM 3D geometry scanner. The scanned results are compared with the FE model of the reference plate. The results presented show the importance of geometrical correlation for modal validation and provide results of deviations that were observed on a reference plate. With these conclusions, working on modal validation can be developed by reducing the inaccuracies for the presentation of correlation. KW - experimental modal analysis KW - impact modal testing KW - validation KW - geometrical correlation KW - 3D geometry scanner KW - FE KW - non-mass loaded impact Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21524 PB - River Publishers ER - TY - CHAP A1 - Mallareddy, Tarun Teja A1 - Schneider, Sarah A1 - Blaschke, Peter T1 - Advanced Hammer Excitation Technique for Impact Modal Testing on Lightweight Materials Using Scalable Automatic Modal Hammer T2 - Topics in Modal Analysis & Testing, Volume 9 : Proceedings of the 36th IMAC, A Conference and Exposition on Structural Dynamics 2018 N2 - Experimental Modal Analysis (EMA) on a lightweight material has proven to be very challenging in the recent past. The applications of these materials have increased invariably in various fields and so have a high demand for Research & Development (R&D). A lightweight material is very sensitive in terms of vibration. EMA on these materials in free - free boundary condition is very complicated as the hammer excitation becomes very difficult. In order to acquire valid results, the conditions are modified, and in consequence, obtain inaccurate dynamic characteristics. Some of the major challenges faced are: (a) material getting displaced from its original position after every hit, (b) difficulties in obtaining a single hit, (c) reproducing the same excitation force level for averaging output response. Overcoming these crucial challenges can result in reducing the inaccuracies in the results. Scalable Automatic Modal hammer (SAM) is developed to overcome these challenges and enables the ability to reproduce the same force level of excitation. This advanced hammer excitation technique has the capability to avoid the double hit, adjust the repeatability of force level and automatizes the entire excitation process. In this research paper, a light weight material is experimented under free-free boundary condition and the obtained results are analyzed. The input hammer excitation is provided by SAM and the output contactless response is measured by Scanning Laser Doppler Vibrometer (SLDV). The conclusions provided will reflect the importance of repeatability and reproducibility of hammer excitation force level in order to acquire accurate results. The controlling of SAM, by changing various parameters, in order to precisely excite lightweight structures will be demonstrated. KW - experimental modal analysis KW - impact modal testing KW - lightweight material testing KW - non-mass-loaded impact KW - validation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-21508 PB - River Publishers ER -