TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Chepurna, Oksana A1 - Grebinyk, Sergii A1 - Prylutskyy, Yuriy A1 - Ritter, Uwe A1 - Ohulchanskyy, Tymish Y. A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex JF - Nanomaterials N2 - A nanosized drug complex was explored to improve the efficiency of cancer chemotherapy, complementing it with nanodelivery and photodynamic therapy. For this, nanomolar amounts of a non-covalent nanocomplex of Doxorubicin (Dox) with carbon nanoparticle C60 fullerene (C60) were applied in 1:1 and 2:1 molar ratio, exploiting C60 both as a drug-carrier and as a photosensitizer. The fluorescence microscopy analysis of human leukemic CCRF-CEM cells, in vitro cancer model, treated with nanocomplexes showed Dox’s nuclear and C60’s extranuclear localization. It gave an opportunity to realize a double hit strategy against cancer cells based on Dox’s antiproliferative activity and C60’s photoinduced pro-oxidant activity. When cells were treated with 2:1 C60-Dox and irradiated at 405 nm the high cytotoxicity of photo-irradiated C60-Dox enabled a nanomolar concentration of Dox and C60 to efficiently kill cancer cells in vitro. The high pro-oxidant and pro-apoptotic efficiency decreased IC50 16, 9 and 7 × 103-fold, if compared with the action of Dox, non-irradiated nanocomplex, and C60’s photodynamic effect, correspondingly. Hereafter, a strong synergy of therapy arising from the combination of C60-mediated Dox delivery and C60 photoexcitation was revealed. Our data indicate that a combination of chemo- and photodynamic therapies with C60-Dox nanoformulation provides a promising synergetic approach for cancer treatment. KW - photodynamic chemotherapy KW - synergistic effect KW - C60 fullerene KW - doxorubicin KW - nanocomplex KW - leukemic cell KW - apoptosis Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12940 SN - 2079-4991 VL - 9 IS - 11 PB - MDPI ER - TY - JOUR A1 - Rösch, Olga A1 - Tolkiehn, Günter-Ulrich A1 - Lehnert, Ralph T1 - Die Landessprache in der Lehre – welche Bedeutung kommt ihr bei der Internationalisierung zu? JF - Die neue Hochschule : DNH N2 - Der Artikel setzt sich kritisch mit dem Trend auseinander, ganze Studiengänge von Landessprache auf Englisch umzustellen und dies als "Internationaliserung" zu bezeichnen. KW - Landessprache KW - Lehre KW - Englisch KW - Internationalisierung Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12984 SN - 0340-488X VL - 2019 IS - 6 SP - 12 EP - 15 ER - TY - JOUR A1 - Steglich, Patrick A1 - Mai, Christian A1 - Villringer, Claus A1 - Mai, Andreas T1 - Direct observation and simultaneous use of linear and quadratic electro-optical effects JF - Journal of Physics D: Applied Physics N2 - We report on the direct observation and simultaneous use of the linear and quadratic electro-optical effect and propose a method by which higher-order susceptibilities of electro-optical materials can be determined. The evaluation is based on the separation of the second- and third-order susceptibilities and the experimental technique uses a slot waveguide ring resonator fabricated in integrated photonic circuit technology, which is embedded by a guest-host polymer system consisting of the azobenzene dye Disperse Red 1 in a poly(methyl methacrylate) matrix as an active electro-optical material. The contribution of both effects on the electro-optical response under the influence of static and time-varying electrical fields is investigated. We show that the quadratic electro-optical effect has a significant influence on the overall electro-optical response even with acentric molecular orientated molecules. Our findings have important implications for developing electro-optical devices based on polymer-filled slot waveguides and give rise to advanced photonic circuits. Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-13032 SN - 1361-6463 VL - 53 IS - 12 ER - TY - JOUR A1 - Lux, Helge A1 - Edling, Matthias A1 - Lucci, Massimiliano A1 - Kitzmann, Julia A1 - Villringer, Claus A1 - Siemroth, Peter A1 - De Matteis, Fabio A1 - Schrader, Sigurd T1 - The Role of Substrate Temperature and Magnetic Filtering for DLC by Cathodic Arc Evaporation JF - Coatings N2 - Diamond-like carbon (DLC) films were deposited using two different types of high current arc evaporation. The first process used a magnetic particle filter to remove droplets from the plasma. For the second process, the samples were put into a metallic cage which was placed directly above the plasma source. For both processes, we varied the substrate temperature from 21 to 350 °C in order to investigate the temperature effect. The samples were characterized using SEM, AFM, XPS, Raman Spectroscopy, Ellipsometry, Photometry, and Nano Indentation in order to compare both methods of deposition and provide a careful characterization of such DLC films. We found that the sp3 content and the hardness can be precisely adjusted by changing the substrate temperature. Furthermore, in the case of unfiltered deposition, the optical constants can be shifted in the direction of higher absorbance in order to produce black and hard carbon coatings. KW - DLC KW - ta-C KW - arc evaporation KW - substrate temperature KW - hardness KW - optical constant KW - ellipsometry Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12295 SN - 2079-6412 VL - 9 IS - 5 SP - 345 ER - TY - JOUR A1 - Steglich, Patrick A1 - Villringer, Claus A1 - Dietzel, Birgit A1 - Mai, Christian A1 - Schrader, Sigurd A1 - Casalboni, Mauro A1 - Mai, Andreas T1 - On-Chip Dispersion Measurement of the Quadratic Electro-Optic Effect in Nonlinear Optical Polymers Using a Photonic Integrated Circuit Technology JF - IEEE Photonics Journal N2 - A novel method to determine the dispersion of the quadratic electro-optic effect in nonlinear optical materials by using a silicon-on-insulator microring resonator is presented. The microring consists of a silicon slot waveguide enabling large dc electric field strength at low applied voltages. The dispersion of third-order hyperpolarizability of a linear conjugated dye is approximated by using a two-level model for the off-resonant spectral region. As an example, the dispersion of the resonance wavelength of the resonator filled with a dye doped polymer was measured in dependence of the applied dc voltage. The polymer was poly (methylmethacrylate) doped with 5 wt% disperse red 1 (DR1), and the measurements have been carried out at the telecommunication wavelength band around 1550 nm (optical C-band). The described measurements represent a new technique to determine the dispersion of the third-order susceptibility and molecular hyperpolarizability of the material filled into the slot of the ring-resonator. KW - electro-optical device KW - nonlinear optics KW - integrated optics KW - integrated optics material KW - optical material KW - integrated optics device KW - polymer active device Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12307 SN - 1943-0655 VL - 11 IS - 3 ER - TY - JOUR A1 - Mikayelyan, Eduard A1 - Grodd, Linda A1 - Ksianzou, Viachaslau A1 - Wesner, Daniel A1 - Rodygin, Alexander I. A1 - Schönherr, Holger A1 - Luponosov, Yuriy N. A1 - Ponomarenko, Sergei A. A1 - Ivanov, Dimitri A. A1 - Pietsch, Ullrich A1 - Grigorian, Souren T1 - Phase Transitions and Formation of a Monolayer-Type Structure in Thin Oligothiophene Films: Exploration with a Combined In Situ X-ray Diffraction and Electrical Measurements JF - Nanoscale Research Letters N2 - A combination of in situ electrical and grazing-incidence X-ray diffraction (GIXD) is a powerful tool for studies of correlations between the microstructure and charge transport in thin organic films. The information provided by such experimental approach can help optimizing the performance of the films as active layers of organic electronic devices. In this work, such combination of techniques was used to investigate the phase transitions in vacuum-deposited thin films of a common organic semiconductor dihexyl-quarterthiophene (DH4T). A transition from the initial highly crystalline phase to a mesophase was detected upon heating, while only a partial backward transition was observed upon cooling to room temperature. In situ electrical conductivity measurements revealed the impact of both transitions on charge transport. This is partly accounted for by the fact that the initial crystalline phase is characterized by inclination of molecules in the plane perpendicular to the π-π stacking direction, whereas the mesophase is built of molecules tilted in the direction of π-π stacking. Importantly, in addition to the two phases of DH4T characteristic of the bulk, a third interfacial substrate-stabilized monolayer-type phase was observed. The existence of such interfacial structure can have important implications for the charge mobility, being especially favorable for lateral two-dimensional charge transport in the organic field-effect transistors geometry. KW - thin film KW - interfacial monolayer KW - oligomer KW - quarterthiophene KW - in situ GIXD KW - phase transition KW - mesophase Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12314 SN - 1556-276X VL - 14 ER - TY - JOUR A1 - Zhao, Shuang A1 - Caruso, Frank A1 - Dähne, Lars A1 - Decher, Gero A1 - De Geest, Bruno G. A1 - Fan, Jinchen A1 - Feliu, Neus A1 - Gogotsi, Yury A1 - Hammond, Paula T. A1 - Hersam, Mark C. A1 - Khademhosseini, Ali A1 - Kotov, Nicholas A1 - Leporatti, Stefano A1 - Li, Yan A1 - Lisdat, Fred A1 - Liz-Marzán, Luis M. A1 - Moya, Sergio A1 - Mulvaney, Paul A1 - Rogach, Andrey L. A1 - Roy, Sathi A1 - Shchukin, Dmitry G. A1 - Skirtach, Andre G. A1 - Stevens, Molly M. A1 - Sukhorukov, Gleb B. A1 - Weiss, Paul S. A1 - Yue, Zhao A1 - Zhu, Dingcheng A1 - Parak, Wolfgang J. T1 - The Future of Layer-by-Layer Assembly: A Tribute to ACS Nano Associate Editor Helmuth Möhwald JF - ACS Nano N2 - Layer-by-layer (LbL) assembly is a widely used tool for engineering materials and coatings. In this Perspective, dedicated to the memory of ACS Nano associate editor Prof. Dr. Helmuth Möhwald, we discuss the developments and applications that are to come in LbL assembly, focusing on coatings, bulk materials, membranes, nanocomposites, and delivery vehicles. Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12356 SN - 1936-086X VL - 13 IS - 6 SP - 6151 EP - 6169 ER - TY - JOUR A1 - Kober, Liane A1 - Hollert, Henner A1 - Frohme, Marcus T1 - Quantification of nitroaromatic explosives in contaminated soil using MALDI-TOF mass spectrometry JF - Analytical and Bioanalytical Chemistry N2 - Contamination from various sources is a global environmental and health threat, with mining and military activities in particular having spread nitroaromatic compounds, such as 2,4,6-trinitrotoluene and its degradation products and by-products, to the soil. The investigation and monitoring of large contaminated areas requires new detection methods since the established ones are expensive and time-consuming. Hence, we established a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI--TOF MS) method using 1,5-diaminonaphthalene as the matrix substance and an internal standard for quantification. Analyzing standard substances, we found specific signals for radical and fragment ions of different nitrotoluenes and nitrobenzenes with good reproducibility and detection limits down to 0.25 ng/μL. The analysis of soil sample extracts from a former production site showed clear signals for 2,4,6-trinitrotoluene and the primary degradation products aminodinitrotoluenes. Furthermore, quantification gave results comparable to those obtained by conventional liquid chromatography--tandem mass spectrometry analysis. The MALDI-TOF MS method has a comparatively lower reproducibility, with relative standard deviations of 6% to 20% for multiple measurements of standard solutions and soil sample extracts. Nevertheless, a comparison of both methods revealed the advantages of MALDI-TOF MS analysis of explosive-contaminated areas with regard to costs, time, and handling. Finally, our MALDI-TOF MS method fulfills all the needs for high sample throughput and can therefore be a valuable screening tool for explosive-contaminated areas. KW - explosives KW - soil contamination KW - TNT KW - matrix-assisted laser desorption/ionization quantification Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12388 SN - 1618-2650 VL - 411 IS - 23 SP - 5993 EP - 6003 ER - TY - JOUR A1 - Gottschalk, Josefine Johanna A1 - Stollfuß, Carsten A1 - Lietzau, Kai-Henning A1 - Foitzik, Andreas A1 - Richetta, Maria T1 - Conceptual study for long-term monitoring of chemotherapeutic induced cell reactions by ESPI JF - tm - Technisches Messen N2 - During the last years, various approaches on an individualized drug therapy for benign cells have been researched. However, due to the complex topic a universal approach has not been found up until this point. Commonly, the effect of cytotoxic drugs on benign cells is in most cases the same compared to regular cells while the actual effect on patient still can't be predicted. In order to reduce unwanted side effects or unspecific drug reactions a test system for patients which allows to analyse the interaction between cytotoxic agents and the targeted cells is needed. Furthermore, this should also include an adequate measurement system which is capable to work in a natural environment and without any additional preparation. In terms of this work, a first proof of concept with different benign cells and cytotoxic agents is presented while monitoring the obtained displacement using electronic speckle pattern interferometry (ESPI). KW - interferometry KW - ESPI KW - anticancer drug KW - cellular displacement Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10676 SN - 2196-7113 VL - 85 IS - 2 SP - 111 EP - 118 ER - TY - JOUR A1 - Bondarenko, Siegfried A1 - Villringer, Claus A1 - Steglich, Patrick T1 - Comparative Study of Nano-Slot Silicon Waveguides Covered by Dye Doped and Undoped Polymer Cladding JF - Applied Sciences N2 - Nonlinear optical dyes doped in optical polymer matrices are widely used for electro-optical devices. Linear optical properties change with dye concentration, which leads to a change in modal properties, especially in nano-structured integrated waveguides such as silicon slot-waveguides. Here, we investigate the influence of a nonlinear optical dye on the performance of a silicon-organic hybrid slot-waveguide. A simulation study of the modal and optical confinement properties is carried out and dependence of the structural parameters of the slot-waveguide and the organic cladding material is taken into account. As cladding material, a guest-host polymer system is employed comprising the nonlinear optical dye Disperse Red 1 (DR1) doped in a poly[methyl methacrylate] (PMMA) matrix. The refractive indices of doped and undoped PMMA were deduced from ellipsometric data. We present a guideline for an optimized slot-waveguide design for the fabrication in silicon-on-insulator technology giving rise to scalable, high-performance integrated electro-optical modulators. KW - silicon photonics KW - modeling and simulation at the nanoscale KW - nonlinear optics at the nanoscale KW - slot waveguide KW - silicon-on-insulator technology Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10716 SN - 2076-3417 VL - 9 IS - 1 PB - MDPI ER - TY - CHAP A1 - Rösch, Olga ED - Rösch, Olga T1 - Zum Thema Technik und Kultur - Anstelle eines Vorwortes T2 - Technik und Kultur N2 - Das Thema der Tagung »Technik und Kultur« hat insbesondere im Kontext einer technischen Hochschule eine unbestreitbare Aktualität. Die technologische Prägung der modernen Welt und der Gegenwartsgesellschaft ist evident: Von den modernen Kommunikationsmedien über die Verkehrsinstrumente, die Errungenschaften der Medizin und Hygiene, die Sicherheitselemente, den Umweltschutz bis hin zur Energie- bzw. Wasserversorgung, zur Ernährung und zur Alltagskultur ist unsere Lebenswelt mittlerweile so sehr von technischen Faktoren geprägt und auch abhängig, dass wir die eigene Geschichte verleugneten, wollten wir diese Dimensionen der Gegenwartskultur(en) ignorieren. KW - Technik und Kultur KW - Interkulturalität KW - Interkulturelle Kommunikation Y1 - 2008 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12071 SN - 978-3-936527-12-4 SP - 10 EP - 15 PB - News & Media CY - Berlin ER - TY - JOUR A1 - Steglich, Patrick A1 - Hülsemann, Marcel A1 - Dietzel, Birgit A1 - Mai, Andreas T1 - Optical Biosensors Based on Silicon-On-Insulator Ring Resonators: A Review JF - Molecules N2 - Recent developments in optical biosensors based on integrated photonic devices are reviewed with a special emphasis on silicon-on-insulator ring resonators. The review is mainly devoted to the following aspects: (1) Principles of sensing mechanism, (2) sensor design, (3) biofunctionalization procedures for specific molecule detection and (4) system integration and measurement set-ups. The inherent challenges of implementing photonics-based biosensors to meet specific requirements of applications in medicine, food analysis, and environmental monitoring are discussed. KW - biosensor KW - biophotonics KW - integrated optical sensor KW - aptamer KW - biomaterial KW - optical sensor KW - silicon photonics KW - ring resonator KW - lab-on-a-chip Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10822 SN - 1420-3049 VL - 24 IS - 3 ER - TY - JOUR A1 - Rösch, Olga T1 - Internationalisierung der Hochschule - was sind unsere Ziele? JF - Die Neue Hochschule N2 - Als ein komplexes Feld lässt sich das Thema Internationalisierung der Hochschulen nach Kehm (2003, S. 8 f.) auf mehreren Bezugsebenen verorten: - Internationalisierung von Studium und Lehre. Hier geht es um die Entwicklung von interkultureller Kompetenz der Beteiligten, um neue Ansätze in der Lehre sowie um Fragen der Qualität. - Herstellung international transparenter und kompatibler Strukturen und Standards. Damit sind die Umstellungen von Studienprogrammen auf gestufte Abschlüsse, qualitative Vergleichbarkeit von Studienleistungen und Akkreditierung von Studiengängen gemeint. - Anpassungen in der Organisation der Hochschulen. Es handelt sich hierbei um die Umstrukturierungen von Akademischen Auslandsämtern und die Einführung einer Leitungsfunktion für Internationale Angelegenheiten. - Internationalisierung des Politikfeldes Hochschulen. Hiermit sind die externen Einwirkungen auf die nationale Bildungspolitik gemeint, beispielsweise durch Organisationen wie OECD, UNESCO, World Bank und Europäische Union mit ihren jeweils „eigenen Definitionen der Funktionen von Hochschulen und der Ziele von Hochschulbildung“ (Kehm 2003, S. 9). Mit diesen Ebenen der Internationalisierung kommen die Lehrenden in unterschiedlicher Intensität in Berührung. Y1 - 2015 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10879 UR - https://hlb.de/fileadmin/hlb-global/downloads/dnh/full/DNH_2015-1.pdf SN - 0340-448x VL - 2015 IS - 1 SP - 18 EP - 24 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Grebinyk, Sergii A1 - Prylutskyy, Yuriy A1 - Ritter, Uwe A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro JF - Nanoscale Research Letters N2 - Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function. The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells. KW - C60 fullerene KW - doxorubicin KW - noncovalent complex KW - leukemic cell KW - cytotoxicity KW - accumulation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10966 SN - 1556-276X N1 - Das Dokument wurde zurückgezogen. Die korrigierte Version ist unter dem folgenden Link zu finden: https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-11415 VL - 14 ER - TY - JOUR A1 - Grebinyk, Anna A1 - Prylutska, Svitlana A1 - Grebinyk, Sergii A1 - Prylutskyy, Yuriy A1 - Ritter, Uwe A1 - Matyshevska, Olga A1 - Dandekar, Thomas A1 - Frohme, Marcus T1 - Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro JF - Nanoscale Research Letters N2 - Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function. The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells. KW - C60 fullerene KW - doxorubicin KW - noncovalent complex KW - leukemic cell KW - cytotoxicity KW - accumulation Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11415 SN - 1556-276X VL - 14 ER - TY - JOUR A1 - Ida, Jeunice A1 - Chan, Soo Khim A1 - Glökler, Jörn A1 - Lim, Yee Ying A1 - Choong, Yee Siew A1 - Lim, Theam Soon T1 - G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing JF - Molecules N2 - G-quadruplexes are made up of guanine-rich RNA and DNA sequences capable of forming noncanonical nucleic acid secondary structures. The base-specific sterical configuration of G-quadruplexes allows the stacked G-tetrads to bind certain planar molecules like hemin (iron (III)-protoporphyrin IX) to regulate enzymatic-like functions such as peroxidase-mimicking activity, hence the use of the term DNAzyme/RNAzyme. This ability has been widely touted as a suitable substitute to conventional enzymatic reporter systems in diagnostics. This review will provide a brief overview of the G-quadruplex architecture as well as the many forms of reporter systems ranging from absorbance to luminescence readouts in various platforms. Furthermore, some challenges and improvements that have been introduced to improve the application of G-quadruplex in diagnostics will be highlighted. As the field of diagnostics has evolved to apply different detection systems, the need for alternative reporter systems such as G-quadruplexes is also paramount. KW - G-quadruplex KW - DNAzyme KW - colorimetric KW - fluorescence KW - luminescence KW - diagnostics Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-11438 SN - 1420-3049 VL - 24 IS - 6 ER - TY - CHAP A1 - Rösch, Olga ED - Rösch, Olga T1 - Deutsche und russische Spiegelbilder T2 - Deutsche und russische Spiegelbilder : Was halten, was erwarten wir voneinander? N2 - „Deutsche und russische Spiegelbilder – eine Reflexion“, in: Publikation des Deutsch-Russischen Forums e.V. zu den 10. Potsdamer Begegnungen: „Deutsche und russische Spiegelbilder. Was halten, was erwarten wir voneinander?“ Y1 - 2008 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12177 SP - 91 EP - 96 PB - Dt.-Russ. Forum CY - Berlin ER - TY - CHAP A1 - Rösch, Olga ED - Rösch, Olga T1 - Neue Identität Polens T2 - Identitäten und interkulturelle Verständigung im Wirtschaftsleben N2 - Eine neue Selbstdefinition Polens in einem veränderten gesellschaftlich-politischen Kontext der erweiterten Europäischen Union, ist eine schlüssige Folge nach den grundlegenden Transformationen der letzten zwei Jahrzehnte in Osteuropa. Die im Land vorherrschende Aufbruchstimmung steht im Einklang mit einem gewachsenen Selbstvertrauen der Polen, was sich u.a. auch in einer nach außen neuen Darstellung der eigenen Identität äußert. Allerdings scheint der Wahrnehmung dieses Wandels auf der deutschen Seite etwas Zwiespältiges anzuhaften. Der Beitritt Polens zur EU hat hierzulande in der Tat ein neues bzw. anderes Interesse für den östlichen Nachbarn geweckt: Polen - als ein Kooperationspartner in politisch ruhigen Zeiten, ein Partner, der einen ganz besonderen Wert darauf legt, in jeder Hinsicht als Gleichberechtigter behandelt zu werden. Das Gebot der Symmetrie in der Kommunikation ist zwar für alle Teilnehmer als Ideal erstrebenswert. Die Realität in der Geschäftskommunikation, vor allem im Wirtschaftsbereich, entspricht verständlicherweise diesem Denkbild noch nicht. Was die deutsch-polnische Kooperation betrifft, so können wir leider nicht sagen, daß die Beziehung so unbeschwert ist wie z.B. zu Portugal oder traditionell so gut funktioniert wie z.B. zu Ungarn. Die Tragödien des 20. Jahrhunderts haben das heutige Selbstverständnis der Deutschen und der Polen nachhaltig geprägt. Y1 - 2004 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12186 SN - 3-932231-10-4 SP - 56 EP - 62 PB - SSIP CY - Berlin ER - TY - CHAP A1 - Rösch, Olga A1 - von Hoerschelmann, Axel ED - Rösch, Olga T1 - Über kulturelle Grenzen – Nachdenken vor der EU-Erweiterung T2 - Osterweiterung der Europäischen Union und kulturelle Grenzen : zur Ausgangslage N2 - In den gesellschaftspolitischen wie akademischen Debatten über Globalisierung ist oft von der zunehmenden Bedeutungslosigkeit der Grenzen von Nationalstaaten die Rede. Diese Feststellung erscheint aus der (welt)- wirtschaftlichen Perspektive auch manifest. Die neuen Informationstechnologien und globalisierten Wirtschaftstätigkeiten führten in den letzten Jahrzehnten dazu, dass – in Worten von Ralf Dahrendorf – „Grenzen fielen, Grenzen der Zeit und des Raumes“. Y1 - 2003 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12206 SN - 3-936527-06-7 SP - 8 EP - 18 PB - Verl. News und Media CY - Berlin ER - TY - JOUR A1 - Rösch, Olga T1 - Anredeformen im Russischen: soziale Domänen und soziale Bedeutungen JF - Fremdsprachenunterricht : fsu ; die Zeitschrift für das Lehren und Lernen fremder Sprachen N2 - Die Vermittlung der fremdkulturellen Kompetenz ist ein integraler Bestandteil des kommunikativen Fremdsprachenunterrichts. Sie beginnt bereits mit dem Erlernen der in der Zielsprache vorhandenen Begrüßungs- und Anredeformen, in denen sich teilweise die Spezifik einer Kultur (Mentalität, soziale Hierarchien, verhaltensbestimmende Wertesysteme, durch Religion geprägte Traditionen) sprachlich manifestiert. Das Anredeverhalten in verschiedenen Sprechergemeinschaften wird von Linguisten und Kommunikationswissenschaftlern vielfach zum Untersuchungsgegenstand gemacht. Die Beschäftigung mit dem Phänomen der Andere und des Begrüßungsrituals der Zielsprache ist aus sprach- und kulturkontrastiver Sicht auch für den Fremdsprachenunterricht von nicht zu unterschätzender Bedeutung. Y1 - 1996 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12248 SN - 0016-0989 VL - 4 SP - 292 EP - 296 ER - TY - CHAP A1 - Rösch, Olga ED - Rösch, Olga T1 - Deutsch-Deutsch-Deutsches : Rußlanddeutsche in Berlin T2 - Deutsch in Osteuropa : ein Tagungsbericht N2 - Die vereinigte, aber noch nicht völlig geinte Berlin ist neben den Problemen des deutsch-deutschen Zusammenwachsens auch mit der Integration eines bemerkbaren Migrantenstroms konfrontiert. Einen nicht geringen Teil der neuen Zuwanderer machen die in der Stadt vor kurzem noch wenig bekannten Rußlanddeutschen aus. Ihre wunschgemäße Zuordnung zum deutschen Kulturkreis ist für einen erheblichen Teil der Berliner problematisch, denn vor allem die sprachlichen Differenzen bis Defizite bei Aussiedlern in bezug auf das Hochdeutsche indizieren, daß es andere, also "fremde" Deutsche sind. Den Aussiedlern fällt es ihrerseits nicht leicht, sich sowohl in der Varietäten des Deutschen als auch in den beiden deutschen Identitäten zurechtzufinden und dabei mit dem Wandlungen ihrer eigenen deutschen - ethnischen, kulturellen und sprachlichen - Selbsverständnissen klarzukommen. Y1 - 1994 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12257 SP - 43 EP - 68 PB - PHVT, Provinciale Hogeschool voor Vertalers en Tolken CY - Gent ER - TY - CHAP A1 - Rösch, Olga ED - Rösch, Olga T1 - Gibt es ein Medium im Deutschen der Gegenwart? T2 - Neue Fragen der Linguistik N2 - Das deutsche verbale Genussystem ist in der Grammatikschreibung zweigliedrig. Den grammatischen Verbformen Aktiv und Passiv werden entsprechend aktivische und passivische Bedeutungen zugeordnet, und somit gelten die semantischen Kategorien Aktiv und Passiv im Gegenwartdeutsch als grammatikalisiert. Der semantischen Kategorie Medium entsprechen im Deutschen keine grammatischen Formparadigmen. Aber auch die Darstellungen des semantischen Begriffes Medium selbst werden in der Literatur eher universalgrammatisch und im engeren Sinne sogar universalsemantisch als einzelsprachlich verstanden. In meiner Untersuchung geht es jedoch nicht primär um das Verständnis des Mediums, sondern um das Verständnis einer bestimmten Konstruktion, die einige Berührungspunkte mit dem früheren Medium aufweist. Die Rede ist von einer formal gekennzeichneten Klasse der Funktionsverbgefüge (FVG), die vielfach als passivische bzw. passivwertige (pw) FVG bezeichnet werden. Y1 - 1991 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-12265 SN - 3-484-30270-4 SP - 253 EP - 261 PB - Niemeyer CY - Tübingen ER - TY - JOUR A1 - Hornemann, Andrea A1 - Sinning, Denise A1 - Cortes, Sofia A1 - Campino, Lenea A1 - Emmer, Peggy A1 - Kuhls, Katrin A1 - Ulm, Gerhard A1 - Frohme, Marcus A1 - Beckhoff, Burkhard T1 - A pilot study on fingerprinting Leishmania species from the Old World using Fourier transform infrared spectroscopy JF - Analytical and Bioanalytical Chemistry N2 - Leishmania species are protozoan parasites and the causative agents of leishmaniasis, a vector borne disease that imposes a large health burden on individuals living mainly in tropical and subtropical regions. Different Leishmania species are responsible for the distinct clinical patterns, such as cutaneous, mucocutaneous, and visceral leishmaniasis, with the latter being potentially fatal if left untreated. For this reason, it is important to perform correct species identification and differentiation. Fourier transform infrared spectroscopy (FTIR) is an analytical spectroscopic technique increasingly being used as a potential tool for identification of microorganisms for diagnostic purposes. By employing mid-infrared (MIR) spectral data, it is not only possible to assess the chemical structures but also to achieve differentiation supported by multivariate statistic analysis. This work comprises a pilot study on differentiation of Leishmania species of the Old World (L. major, L. tropica, L. infantum, and L. donovani) as well as hybrids of distinct species by using vibrational spectroscopic fingerprints. Films of intact Leishmania parasites and their deoxyribonucleic acid (DNA) were characterized comparatively with respect to their biochemical nature and MIR spectral patterns. The strains’ hyperspectral datasets were multivariately examined by means of variance-based principal components analysis (PCA) and distance-based hierarchical cluster analysis (HCA). With the implementation of MIR spectral datasets we show that a phenotypic differentiation of Leishmania at species and intra-species level is feasible. Thus, FTIR spectroscopy can be further exploited for building up spectral databases of Leishmania parasites in view of high-throughput analysis of clinical specimens. KW - Fourier transform infrared spectroscopy KW - hierarchical cluster analysis (HCA) KW - principal components analysis (PCA) KW - Leishmania KW - DNA KW - multivariate differentiation Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10080 SN - 1432-1130 ER -