TY - JOUR A1 - Glös, Franziska A1 - Böhme, Andrea A1 - Liebscher, Thilo A1 - Zinn, Steffen A1 - Richetta, Maria A1 - Foitzik, Andreas T1 - Analyte Tracking for Novel Bio-Applications JF - Materials Science Forum N2 - Modern cell culture as well as sophisticated bio-applications involve complex biochemical processes, which are required to induce growth, product development or material degradation. Tracking the reaction processes inside the application presents a major challenge due to its complexity. The development of new analysis and tracking mechanisms for such application presents a solution to fully understand the process. In addition, the applied sensors are required to monitor the reactions enable a live tracking of the process. Furthermore, this gives the opportunity to influence and manipulate reactions to further enhance the application of the process. Possible analytes for tracking during processes can be chemical origin such as glucose, cytokines, antibiotics and growth factors, which are included in the culture medium. Based on the complexity of the culture or bio-application the sensor tracking mechanism has to be adapted to ensure full process control. A variety of different approaches can be used for the tracking mechanism. KW - bio application KW - polymer material KW - sample tracking Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18676 SN - 0255-5476 SN - 1662-9752 VL - 941 SP - 2454 EP - 2457 PB - Trans Tech Publications ER - TY - JOUR A1 - Lobstein, Susanne A1 - Zinn, Steffen A1 - Minieri, Marilena A1 - Foitzik, Andreas T1 - Sample Analysis for Novel Medical Applications JF - Materials Science Forum N2 - The sample preparation for biological and chemical probes involves following a strict workflow to eliminate any contamination to the sample beforehand. Furthermore, it is time consuming and must be carried out by trained personnel such as a nurse or other supervisors, making it therefore expensive. The development of novel sample preparation techniques paired with modern sample analysis systems is focused on improving the operability while keeping a constant quality of results. This is important to analyse samples, which cannot be determined with current screening conditions. The analysis of analytes is required to receive a more detailed picture of the patient and to fully understand its complexity. Possible samples for in-depth analysis of chemical origin can be cholesterol or glucose. More complex samples, such as blood or saliva, require a sophisticated system, which analyses the samples for their individual compounds. Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18749 SN - 0255-5476 SN - 1662-9752 VL - 941 SP - 2518 EP - 2521 PB - Trans Tech Publications ER - TY - JOUR A1 - Engels, Andreas A1 - Zinn, Steffen A1 - Bonaiuto, Vincenzo A1 - Foitzik, Andreas T1 - Improved Syringe Extruder for Improved Processing of Biological Materials JF - Materials Science Forum N2 - 3 dimensional (3D) printing evolved during the last decade to a consumer friendly and affordable craft. Furthermore, implementations of this techniques in the field of biotechnological research and development within laboratories is a very expansive process. Bio-printers’ prices cover a wide spectrum and most basic models are available for around 5000€. On the other end, high-end printer machines with a vast variety of features are available for several hundreds of thousands of euros. Thus, due to the immense potential in the field of Biotechnology the availability of this technology for research purpose should be enhanced. A developed ecological syringe extruder prototype for processing of biological based gels has been further improved. The original prototype was capable to processing multiple layers of agar with concentrations of 1% and 2.5%. Based on these results the prototype was revised regarding printing process parameter, which include among others applied forces to the substrate, air-ventilation, and heating of the substrate. The process behavior will be simulated with computational fluid dynamics for the processing of biological based substrate. After a concluding validation these results are intended to be implemented into a new design for improved processing of a variety of bioinks. KW - biofabrication KW - bio-ink KW - bioprinting KW - Open Source KW - process parameter improvement Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18701 SN - 0255-5476 SN - 1662-9752 VL - 941 SP - 2442 EP - 2447 PB - Trans Tech Publications ER - TY - JOUR A1 - Rogge, Christian A1 - Zinn, Steffen A1 - Prosposito, Paolo A1 - Francini, Roberto A1 - Foitzik, Andreas T1 - Transmitted light pH optode for small sample volumes JF - Journal of Sensors and Sensor Systems N2 - An innovative concept of a low-cost pH optode with working volumes of less than 150 µL is presented. The pH monitoring is based on the color changing effect of pH indicators. The optode includes an RGB color sensor patch TCS34725 from Adafruit, a controllable LED and reactor slides and is addressed by a self-written LabVIEW© software. Utilizing the hue value of the HSV color model, it is possible to analyze the color change of the indicator and estimate the pH value of the analyzed samples by exploiting sigmoidal fit models. Measurements carried out with phenol red and DMEM (Dulbecco's Modified Eagle's Medium) reported a standard error of calibration in the physiologic pH range (6.5–7.5) of ±0.04 pH units. Y1 - 2017 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-10093 SN - 2194-878X VL - 6 IS - 2 SP - 351 EP - 359 ER -