TY - JOUR A1 - Glös, Franziska A1 - Böhme, Andrea A1 - Liebscher, Thilo A1 - Zinn, Steffen A1 - Richetta, Maria A1 - Foitzik, Andreas T1 - Analyte Tracking for Novel Bio-Applications JF - Materials Science Forum N2 - Modern cell culture as well as sophisticated bio-applications involve complex biochemical processes, which are required to induce growth, product development or material degradation. Tracking the reaction processes inside the application presents a major challenge due to its complexity. The development of new analysis and tracking mechanisms for such application presents a solution to fully understand the process. In addition, the applied sensors are required to monitor the reactions enable a live tracking of the process. Furthermore, this gives the opportunity to influence and manipulate reactions to further enhance the application of the process. Possible analytes for tracking during processes can be chemical origin such as glucose, cytokines, antibiotics and growth factors, which are included in the culture medium. Based on the complexity of the culture or bio-application the sensor tracking mechanism has to be adapted to ensure full process control. A variety of different approaches can be used for the tracking mechanism. KW - bio application KW - polymer material KW - sample tracking Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18676 SN - 0255-5476 SN - 1662-9752 VL - 941 SP - 2454 EP - 2457 PB - Trans Tech Publications ER - TY - JOUR A1 - Matuschek, Nelson A1 - Liebscher, Thilo A1 - Santo, Loredana A1 - Foitzik, Andreas T1 - Online Glucose Measurement in DMEM for In Vitro Cell Culture JF - Materials Science Forum N2 - In-vitro cell culture offers the ability to grow individual cells and monitor their behaviour in a controlled environment over a certain time. During culture, different parameters have to be controlled to ensure a vital growth of cells. The most important parameters are temperature, pH-value, oxygen and carbon dioxide levels as well as the glucose concentration. All of these parameters influence the growth ability of the cell culture and should be monitored online. The online monitoring of glucose was achieved with a novel GOD based sensor which enabled a real-time measurement of glucose during cell culture. A first-time characterisation of the sensor was carried out in the culture medium DMEM. In addition, material properties of the culture system were investigated. At first to establish a baseline measurement, the sensor was placed in different glucose concentrations dissolved in Water-KCl mixture at 25 °C inside a batch chamber under constant stirring. Afterwards, the temperature was increased to 37 °C to simulate a cell culture environment. In addition, the system was transferred into a flow-through reaction chamber. The highest response signal and the most stable signal was achieved at 37 °C with DMEM during flow-through measurement. Based on these measurements online glucose monitoring in cell culture was possible to determine the glucose consumption for each cell which is important for future human-on-a-chip devices to prevent diabetic metabolisms. KW - biosensor KW - cell culture KW - cell KW - micro-reactor KW - online glucose monitoring Y1 - 2018 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-18734 SN - 0255-5476 SN - 1662-9752 VL - 941 SP - 2501 EP - 2506 PB - Trans Tech Publications ER -