TY - JOUR A1 - Baumann, Elisabeth A1 - Pohle, Ulrike A1 - Zhang, Edward A1 - Allen, Thomas A1 - Villringer, Claus A1 - Pulwer, Silvio A1 - Gerhardt, Holger A1 - Laufer, Jan T1 - A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor JF - Photoacoustics N2 - Optical-resolution photoacoustic microscopy (OR-PAM) combines high spatial resolution and strong absorption-based contrast in tissue, which has enabled structural and spectroscopic imaging of endogenous chromophores, primarily hemoglobin. Conventional piezoelectric ultrasound transducers are typically placed far away from the photoacoustic source due to their opacity, which reduces acoustic sensitivity. Optical ultrasound sensors are an alternative as their transparency allows them to be positioned close to the sample with minimal source-detector distances. In this work, a backward-mode OR-PAM system based on a planar Fabry-Pérot ultrasound sensor and coaxially aligned excitation and interrogation beams was developed. Two 3D imaging modes, using raster-scanning for enhanced image quality and continuous-scanning for fast imaging, were implemented and tested on a leaf skeleton phantom. In fast imaging mode, a scan-rate of 100,000 A-lines/s was achieved. 3D images of a zebrafish embryo were acquired in vivo in raster-scanning mode. The transparency of the FP sensor in the visible and near-infrared wavelength region makes it suitable for combined functional and molecular imaging applications using OR-PAM and multi-photon fluorescence microscopy. KW - optical-resolution photoacoustic microscopy KW - backward-mode imaging KW - dual-wavelength KW - continuous scanning KW - in vivo imaging KW - optical ultrasound sensing KW - planar Fabry-Pérot interferometer Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:526-opus4-14701 SN - 2213-5979 VL - 24 SP - 100293 PB - Elsevier ER -