652 2009 eng 1287 1290 1 1 article 1 -- -- -- Several mutation positions have been chosen for introducing positively charged lysines in human cytochrome c (cyt c) with the aim of increasing the reaction rate with superoxide radicals (SO) and thus, the sensitivity of an electrochemical cyt c based SO biosensor. The impact of the mutations on structural and redox properties as well as on the reaction rate with SO are verified. Four mutants show a higher reaction rate with the radical compared to the wild type. These mutants are used for the construction of SO sensors based on thiol-modified gold electrodes and covalently fixed proteins. The E66K mutant electrode has a clearly higher sensitivity in comparison to the wildtype based sensor. Procedia Chemistry 1876-6196 urn:nbn:de:kobv:526-opus4-6523 F. Wegerich et al. Superoxide Biosensing with Engineered Cytochrome c. Procedia Chemistry 1 (2009) 1287–1290. doi:10.1016/j.proche.2009.07.321 https://doi.org/10.1016/j.proche.2009.07.321 Creative Commons - CC BY-NC-ND 3.0 - Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Unported F. Wegerich Paola Turano Marco Allegrozzi H. Möhwald Fred Lisdat eng uncontrolled biosensor eng uncontrolled cytochrome c eng uncontrolled superoxide eng uncontrolled protein engineering Chemie und zugeordnete Wissenschaften Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014) open_access Gold Open Access Technische Hochschule Wildau https://opus4.kobv.de/opus4-th-wildau/files/652/1-s2.0-S1876619609003222-main.pdf