639 2012 eng 25 9 7 article 1 -- -- -- Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state. PLoS ONE 1932-6203 urn:nbn:de:kobv:526-opus4-6392 Schokraie E, Warnken U, Hotz-Wagenblatt A, Grohme MA, Hengherr S, et al. (2012) Comparative proteome analysis ofMilnesium tardigradumin earlyembryonic stateversusadults in active and anhydrobiotic state. PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682 https://doi.org/10.1371/journal.pone.0045682 Creative Commons - CC BY - Namensnennung 2.0 Generic Elham Schokraie Uwe Warnken Agnes Hotz-Wagenblatt Markus Grohme Steffen Hengherr Frank Förster Ralph O. Schill Marcus Frohme Thomas Dandekar Martina Schnölzer Biowissenschaften; Biologie Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014) open_access Gold Open Access Technische Hochschule Wildau https://opus4.kobv.de/opus4-th-wildau/files/639/journal.pone.0045682.pdf