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    <id>1929</id>
    <completedYear>2024</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>160</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
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    <title language="eng">Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures</title>
    <abstract language="eng">The pattern of the activity of proteases is related to distinct physiological states of living organisms. Often activity changes of a certain protease can be assigned to a specific disease. Hence, they are useful biomarkers and a simple and fast determination method of their activity could be a valuable tool for the efficient monitoring of numerous diseases. Here, two different methods for the qualitative and quantitative determination of protease activity are demonstrated using the model system of proteinase K. The first test system is based on a protein-modified and colored 3D silica structure that changes color when exposed to the enzyme. This method has also been used for the detection of matrix metallo-protease 2 (MMP2) with gelatine as protease substrate on the plates. The second detection system uses the decrease in the voltammetric signal of a cytochrome c/DNA multilayer electrode after incubation with a protease to quantitatively determine its proteolytic activity. While activities down to 0.15 U/ml can be detected with the first method, the second one provides detection limits of about 0.03 U/ml (for proteinase K.) The functionality of both systems can be demonstrated and ways for further enhancement of sensitivity have been elucidated.</abstract>
    <parentTitle language="eng">Bioelectrochemistry</parentTitle>
    <identifier type="issn">1567-5394</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-19294</identifier>
    <enrichment key="opus.import.data">@articleGöbel2024, address = "Amsterdam", publisher = "Elsevier", issn = "1567-5394", eissn = "1878-562X", abstract = "The pattern of the activity of proteases is related to distinct physiological states of living organisms. Often activity changes of a certain protease can be assigned to a specific disease. Hence, they are useful biomarkers and a simple and fast determination method of their activity could be a valuable tool for the efficient monitoring of numerous diseases. Here, two different methods for the qualitative and quantitative determination of protease activity are demonstrated using the model system of proteinase K. The first test system is based on a protein-modified and colored 3D silica structure that changes color when exposed to the enzyme. This method has also been used for the detection of matrix metallo-protease 2 (MMP2) with gelatine as protease substrate on the plates. The second detection system uses the decrease in the voltammetric signal of a cytochrome c/DNA multilayer electrode after incubation with a protease to quantitatively determine its proteolytic activity. While activities down to 0.15 U/ml can be detected with the first method, the second one provides detection limits of about 0.03U/ml (for proteinase K.) The functionality of both systems can be demonstrated and ways for further enhancement of sensitivity have been elucidated.", author = "Göbel, Gero and Müller, Florian and Talke, Anja and Ahnert, Uwe and Lisdat, Fred", doi = "10.1016/j.bioelechem.2024.108775", journal = "Bioelectrochemistry", keywords = "Multilayer electrode, Protease activity, Proteinase K, Silica gel", language = "eng", pages = "108775", title = "Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures", volume = "160", year = "2024"</enrichment>
    <enrichment key="opus.import.dataHash">md5:123024a853cf042176dbcce315a469b8</enrichment>
    <enrichment key="opus.import.date">2024-07-18T07:25:21+00:00</enrichment>
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    <enrichment key="opus.import.id">6698c3616655e2.92728366</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1016/j.bioelechem.2024.108775</enrichment>
    <enrichment key="SourceTitle">Göbel, G., Müller, F., Talke, A., Ahnert, U., &amp; Lisdat, F. (2024). Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures. Bioelectrochemistry, 160, 108775. doi:10.1016/j.bioelechem.2024.108775</enrichment>
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    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Gero Göbel</author>
    <author>Florian Müller</author>
    <author>Anja Talke</author>
    <author>Uwe Ahnert</author>
    <author>Fred Lisdat</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>multilayer electrode</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>protease activity</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>proteinase K</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>silica gel</value>
    </subject>
    <collection role="ddc" number="572">Biochemie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1929/1-s2.0-S1567539424001373-main.pdf</file>
  </doc>
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