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  <doc>
    <id>1058</id>
    <completedYear>2018</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>319</pageFirst>
    <pageLast>327</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>124</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">C60 fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy</title>
    <abstract language="eng">Recent progress in nanobiotechnology has attracted interest to a biomedical application of the carbon nanostructure C60 fullerene since it possesses a unique structure and versatile biological activity. C60 fullerene potential application in the frame of cancer photodynamic therapy (PDT) relies on rapid development of new light sources as well as on better understanding of the fullerene interaction with cells.&#13;
&#13;
The aim of this study was to analyze C60 fullerene effects on human leukemic cells (CCRF-CEM) in combination with high power single chip light-emitting diodes (LEDs) light irradiation of different wavelengths: ultraviolet (UV, 365 nm), violet (405 nm), green (515 nm) and red (632 nm). The time-dependent accumulation of fullerene C60 in CCRF-CEM cells up to 250 ng/106 cells at 24 h with predominant localization within mitochondria was demonstrated with immunocytochemical staining and liquid chromatography mass spectrometry. In a cell viability assay we studied photoexcitation of the accumulated C60 nanostructures with ultraviolet or violet LEDs and could prove that significant phototoxic effects did arise. A less pronounced C60 fullerene phototoxic effect was observed after irradiation with green, and no effect was detected with red light. A C60 fullerene photoactivation with violet light induced substantial ROS generation and apoptotic cell death, confirmed by caspase3/7 activation and plasma membrane phosphatidylserine externalization. Our work proved C60 fullerene ability to induce apoptosis of leukemic cells after photoexcitation with high power single chip 405 nm LED as a light source. This underlined the potential for application of C60 nanostructure as a photosensitizer for anticancer therapy.</abstract>
    <parentTitle language="eng">Free Radical Biology and Medicine</parentTitle>
    <identifier type="issn">1873-4596</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-10588</identifier>
    <enrichment key="SourceTitle">Grebinyk, A. et al. (2018). C60 fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy Free Radical Biology and Medicine. 124, 319-327.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1016/j.freeradbiomed.2018.06.022</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Sergii Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Uwe Ritter</author>
    <author>Olga Matyshevska</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1058/1-s2.0-S0891584918311043-main.pdf</file>
  </doc>
  <doc>
    <id>2009</id>
    <completedYear>2025</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>5</issue>
    <volume>70</volume>
    <type>article</type>
    <publisherName>Institute of Physics Publishing (IOP)</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Demonstration of ultra-high dose rate electron irradiation at FLASHlab@PITZ</title>
    <abstract language="eng">Objective. The photo injector test facility at DESY in Zeuthen (PITZ) is building up an R&amp;D platform, known as FLASHlab@PITZ, for systematically studying the FLASH effect in cancer treatment with its high-brightness electron beams, which can provide a uniquely large dose parameter range for radiation experiments. In this paper, we demonstrate the capabilities by experiments with a reduced parameter range on a startup beamline and study the potential performance of the full beamline by simulations. Approach. To measure the dose, Gafchromic films are installed both in front of and after the samples; Monte Carlo simulations are conducted to predict the dose distribution during beam preparation and help understand the dose distribution inside the sample. Plasmid DNA is irradiated under various doses at conventional and ultra-high dose rate (UHDR) to study the DNA damage by radiations. Start-to-end simulations are performed to verify the performance of the full beamline. Main results. On the startup beamline, reproducible irradiation has been established with optimized electron beams and the delivered dose distributions have been measured with Gafchromic films and compared to FLUKA simulations. The functionality of this setup has been further demonstrated in biochemical experiments at conventional dose rate of 0.05 Gy s−1 and UHDR of several 105 Gy s−1 and a varying dose up to 60 Gy, with the UHDR experiments finished within a single RF pulse (less than 1 millisecond); the observed conformation yields of the irradiated plasmid DNA revealed its dose-dependent radiation damage. The upgrade to the full FLASHlab@PITZ beamline is justified by simulations with homogeneous radiation fields generated by both pencil beam scanning and scattering beams. Significance. With the demonstration of UHDR irradiation and the simulated performance of the new beamline, FLASHlab@PITZ will serve as a powerful platform for studying the FLASH effects in cancer treatment.</abstract>
    <parentTitle language="eng">Physics in Medicine &amp; Biology</parentTitle>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-20092</identifier>
    <enrichment key="opus.import.date">2025-02-25T12:19:32+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1088/1361-6560/adb276</enrichment>
    <enrichment key="SourceTitle">X-K Li et al 2025 Phys. Med. Biol. 70 055010</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Xiangkun Li</author>
    <author>Zohrab Amirkhanyan</author>
    <author>Anna Grebinyk</author>
    <author>Matthias Gross</author>
    <author>Yuliia Komar</author>
    <author>Felix Riemer</author>
    <author>Aida Asoyan</author>
    <author>Prach Boonpornprasert</author>
    <author>Paul Borchert</author>
    <author>Hakob Davtyan</author>
    <author>Dmytro Dmytriiev</author>
    <author>Marcus Frohme</author>
    <author>Andreas Hoffmann</author>
    <author>Mikhail Krasilnikov</author>
    <author>Gregor Loisch</author>
    <author>Zahra Lotfi</author>
    <author>Frieder Müller</author>
    <author>Michael Schmitz</author>
    <author>Frank Obier</author>
    <author>Anne Oppelt</author>
    <author>Sebastian Philipp</author>
    <author>Christopher Richard</author>
    <author>Grygorii Vashchenko</author>
    <author>Daniel Villani</author>
    <author>Steven Worm</author>
    <author>Frank Stephan</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ultra-high dose rate</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>FLASH effect</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>electron beams</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>FLASHlab@PITZ</value>
    </subject>
    <collection role="ddc" number="539">Moderne Physik</collection>
    <collection role="ddc" number="615">Pharmakologie, Therapeutik</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/2009/Li_2025_Phys._Med._Biol._70_055010.pdf</file>
  </doc>
  <doc>
    <id>1610</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>945</pageFirst>
    <pageLast>956</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>9</volume>
    <type>article</type>
    <publisherName>Springer</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>2019-07-01</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A new triple system DNA-Nanosilver-Berberine for cancer therapy</title>
    <abstract language="eng">The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA. In the current work, we have investigated the effects of Berberine on the human T cell leukemia cell line in vitro. Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time- and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction. Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers. The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanoparticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment.</abstract>
    <parentTitle language="eng">Applied Nanoscience</parentTitle>
    <identifier type="issn">2190-5517</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-16105</identifier>
    <enrichment key="opus.import.date">2022-05-12T12:02:28+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.file">filename=phpC64CwN</enrichment>
    <enrichment key="opus.import.checksum">96b3eedaa2d4689c61de21fc683c79fa</enrichment>
    <enrichment key="SourceTitle">Grebinyk, A., Yashchuk, V., Bashmakova, N. et al. A new triple system DNA-Nanosilver-Berberine for cancer therapy. Appl Nanosci 9, 945–956 (2019). https://doi.org/10.1007/s13204-018-0688-x</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s13204-018-0688-x</enrichment>
    <enrichment key="RelatedIdentifier">https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/1062</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Valeriy Yashchuk</author>
    <author>Nataliya Bashmakova</author>
    <author>Dmytro Gryn</author>
    <author>Tobias Hagemann</author>
    <author>Antonina Naumenko</author>
    <author>Nataliya Kutsevol</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1610/Grebinyk2019_Article.pdf</file>
  </doc>
</export-example>
