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  <doc>
    <id>1474</id>
    <completedYear>2021</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>24</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>12</volume>
    <type>article</type>
    <publisherName>BioMed Central</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Antitumor efficiency of the natural alkaloid berberine complexed with C60 fullerene in Lewis lung carcinoma in vitro and in vivo</title>
    <abstract language="eng">Background&#13;
Berberine (Ber) is a herbal alkaloid with pharmacological activity in general and a high anticancer potency in particular. However, due to its low bioavailability, the difficulty in reaching a target and choosing the right dose, there is a need to improve approaches of Ber use in anticancer therapy. In this study, Ber, noncovalently bound to a carbon nanostructure C60 fullerene (C60) at various molar ratios of the components, was explored against Lewis lung carcinoma (LLC).&#13;
&#13;
Methods&#13;
C60–Ber noncovalent nanocomplexes were synthesized in 1:2, 1:1 and 2:1 molar ratios. Ber release from the nanocomplexes was studied after prolonged incubation at different pH with the liquid chromatography–mass spectrometry analysis of free Ber content. Biological effects of the free and C60-complaxated Ber were studied in vitro towards LLC cells with phase-contrast and fluorescence microscopy, flow cytometry, MTT reduction, caspase activity and wound closure assays. The treatment with C60–Ber nanocomplex was evaluated in vivo with the LLC-tumored C57Bl mice. The mice body weight, tumor size, tumor weight and tumor weight index were assessed for four groups, treated with saline, 15 mg C60/kg, 7.5 mg Ber/kg or 2:1 C60-Ber nanocomplex (15 mg C60/kg, 7.5 mg Ber/kg).&#13;
&#13;
Results&#13;
Ber release from C60–Ber nanocomplexes was promoted with medium acidification. LLC cells treatment with C60–Ber nanocomplexes was followed by enhanced Ber intracellular uptake as compared to free Ber. The cytotoxicity of the studied agents followed the order: free Ber &lt; 1:2 &lt; 1:1 &lt; 2:1 C60–Ber nanocomplex. The potency of cytotoxic effect of 2:1 C60–Ber nanocomplex was confirmed by 21.3-fold decrease of IC50 value (0.8 ± 0.3 µM) compared to IC50 for free Ber (17 ± 2 µM). C60–Ber nanocomplexes induced caspase 3/7 activation and suppressed the migration activity of LLC cells. The therapeutic potency of 2:1 C60–Ber nanocomplex was confirmed in a mouse model of LLC. The tumor growth in the group treated with 2:1 C60–Ber nanocomplex is suppressed by approximately 50% at the end of experiment, while in the tumor-bearing group treated with free Ber no therapeutic effect was detected.&#13;
&#13;
Conclusions&#13;
This study indicates that complexation of natural alkaloid Ber with C60 may be a novel therapeutic strategy against lung carcinoma.</abstract>
    <parentTitle language="eng">Cancer Nanotechnology</parentTitle>
    <identifier type="issn">1868-6966</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-14749</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="SourceTitle">Grebinyk, A., Prylutska, S., Grebinyk, S. et al. Antitumor efficiency of the natural alkaloid berberine complexed with C60 fullerene in Lewis lung carcinoma in vitro and in vivo. Cancer Nano 12, 24 (2021). https://doi.org/10.1186/s12645-021-00096-6</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/s12645-021-00096-6</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Sergii Grebinyk</author>
    <author>Maxim Evstigneev</author>
    <author>Iryna Krysiuk</author>
    <author>Tetiana Skaterna</author>
    <author>Iryna Horak</author>
    <author>Yanfang Sun</author>
    <author>Liudmyla Drobot</author>
    <author>Olga Matyshevska</author>
    <author>Yuriy Prylutskyy</author>
    <author>Uwe Ritter</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Funding" number="">Projekt DEAL</collection>
    <collection role="Funding" number="">Publikationsfonds der TH Wildau</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1474/s12645-021-00096-6.pdf</file>
  </doc>
  <doc>
    <id>1687</id>
    <completedYear>2023</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>2</issue>
    <volume>24</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells</title>
    <abstract language="eng">The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C60 fullerene (C60), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C60 effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C60 in HeLa cells reached its maximum at 24 h (800 ± 66 ng/106 cells). Half of extranuclear C60 is localized within mitochondria. The efficiency of the C60 nanostructure’s sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C60 was found for HeLa cells. C60′s ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment.</abstract>
    <parentTitle language="eng">International Journal of Molecular Sciences</parentTitle>
    <identifier type="issn">1422-0067</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-16877</identifier>
    <enrichment key="opus.import.data">@Articleijms24021020, AUTHOR = Radivoievych, Aleksandar and Kolp, Benjamin and Grebinyk, Sergii and Prylutska, Svitlana and Ritter, Uwe and Zolk, Oliver and Glökler, Jörn and Frohme, Marcus and Grebinyk, Anna, TITLE = Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells, JOURNAL = International Journal of Molecular Sciences, VOLUME = 24, YEAR = 2023, NUMBER = 2, ARTICLE-NUMBER = 1020, URL = https://www.mdpi.com/1422-0067/24/2/1020, ISSN = 1422-0067, ABSTRACT = The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C60 fullerene (C60), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C60 effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C60 in HeLa cells reached its maximum at 24 h (800 &amp;plusmn; 66 ng/106 cells). Half of extranuclear C60 is localized within mitochondria. The efficiency of the C60 nanostructure&amp;rsquo;s sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C60 was found for HeLa cells. C60&amp;prime;s ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment., DOI = 10.3390/ijms24021020</enrichment>
    <enrichment key="opus.import.dataHash">md5:76c5aa0796f241835d6d5c549fa910cb</enrichment>
    <enrichment key="opus.import.date">2023-01-09T08:15:26+00:00</enrichment>
    <enrichment key="opus.import.file">/tmp/phptq6JS9</enrichment>
    <enrichment key="opus.import.format">bibtex</enrichment>
    <enrichment key="opus.import.id">63bbcd1e4ffdd2.46312195</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.3390/ijms24021020</enrichment>
    <enrichment key="SourceTitle">Radivoievych, A.; Kolp, B.; Grebinyk, S.; Prylutska, S.; Ritter, U.; Zolk, O.; Glökler, J.; Frohme, M.; Grebinyk, A. Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells. Int. J. Mol. Sci. 2023, 24, 1020. https://doi.org/10.3390/ijms24021020</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Aleksandar Radivoievych</author>
    <author>Benjamin Kolp</author>
    <author>Sergii Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Uwe Ritter</author>
    <author>Oliver Zolk</author>
    <author>Jörn Glökler</author>
    <author>Marcus Frohme</author>
    <author>Anna Grebinyk</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ultrasound</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>C60 fullerene</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>sonodynamic therapy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>HeLa cells</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>apoptosis</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="ddc" number="616">Krankheiten</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Funding" number="">Publikationsfonds der TH Wildau</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1687/ijms-24-01020.pdf</file>
  </doc>
  <doc>
    <id>1674</id>
    <completedYear>2022</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>5077</pageFirst>
    <pageLast>5088</pageLast>
    <pageNumber/>
    <edition/>
    <issue>23</issue>
    <volume>4</volume>
    <type>article</type>
    <publisherName>Royal Society of Chemistry (RSC)</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer</title>
    <abstract language="eng">The development of precision cancer medicine relies on novel formulation strategies for targeted drug delivery to increase the therapeutic outcome. Biocompatible polymer nanoparticles, namely dextran-graft-polyacrylamide (D-g-PAA) copolymers, represent one of the innovative non-invasive approaches for drug delivery applications in cancer therapy. In this study, the star-like D-g-PAA copolymer in anionic form (D-g-PAAan) was developed for pH-triggered targeted drug delivery of the common chemotherapeutic drugs – doxorubicin (Dox) and cisplatin (Cis). The initial D-g-PAA copolymer was synthesized by the radical graft polymerization method, and then alkaline-hydrolyzed to get this polymer in anionic form for further use for drug encapsulation. The acidification of the buffer promoted the release of loaded drugs. D-g-PAAan nanoparticles increased the toxic potential of the drugs against human and mouse lung carcinoma cells (A549 and LLC), but not against normal human lung cells (HEL299). The drug-loaded D-g-PAAan-nanoparticles promoted further oxidative stress and apoptosis induction in LLC cells. D-g-PAAan-nanoparticles improved Dox accumulation and drugs’ toxicity in a 3D LLC multi-cellular spheroid model. The data obtained indicate that the strategy of chemotherapeutic drug encapsulation within the branched D-g-PAAan nanoparticle allows not only to realize pH-triggered drug release but also to potentiate its cytotoxic, prooxidant and proapoptotic effects against lung carcinoma cells.</abstract>
    <parentTitle language="eng">Nanoscale Advances</parentTitle>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-16749</identifier>
    <enrichment key="opus.import.data">@ArticleD2NA00353H, author ="Grebinyk, Anna and Prylutska, Svitlana and Grebinyk, Sergii and Ponomarenko, Stanislav and Virych, Pavlo and Chumachenko, Vasyl and Kutsevol, Nataliya and Prylutskyy, Yuriy and Ritter, Uwe and Frohme, Marcus", title ="Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer", journal ="Nanoscale Adv.", year ="2022", volume ="4", issue ="23", pages ="5077-5088", publisher ="RSC", doi ="10.1039/D2NA00353H", url ="http://dx.doi.org/10.1039/D2NA00353H", abstract ="The development of precision cancer medicine relies on novel formulation strategies for targeted drug delivery to increase the therapeutic outcome. Biocompatible polymer nanoparticles, namely dextran-graft-polyacrylamide (D-g-PAA) copolymers, represent one of the innovative non-invasive approaches for drug delivery applications in cancer therapy. In this study, the star-like D-g-PAA copolymer in anionic form (D-g-PAAan) was developed for pH-triggered targeted drug delivery of the common chemotherapeutic drugs – doxorubicin (Dox) and cisplatin (Cis). The initial D-g-PAA copolymer was synthesized by the radical graft polymerization method, and then alkaline-hydrolyzed to get this polymer in anionic form for further use for drug encapsulation. The acidification of the buffer promoted the release of loaded drugs. D-g-PAAan nanoparticles increased the toxic potential of the drugs against human and mouse lung carcinoma cells (A549 and LLC), but not against normal human lung cells (HEL299). The drug-loaded D-g-PAAan-nanoparticles promoted further oxidative stress and apoptosis induction in LLC cells. D-g-PAAan-nanoparticles improved Dox accumulation and drugs’ toxicity in a 3D LLC multi-cellular spheroid model. The data obtained indicate that the strategy of chemotherapeutic drug encapsulation within the branched D-g-PAAan nanoparticle allows not only to realize pH-triggered drug release but also to potentiate its cytotoxic, prooxidant and proapoptotic effects against lung carcinoma cells."</enrichment>
    <enrichment key="opus.import.dataHash">md5:f6a8c3c8e0076062674cfacef4031ed7</enrichment>
    <enrichment key="opus.import.date">2022-11-25T11:39:42+00:00</enrichment>
    <enrichment key="opus.import.file">/tmp/phpshl2n6</enrichment>
    <enrichment key="opus.import.format">bibtex</enrichment>
    <enrichment key="opus.import.id">6380a97e77fba6.10639762</enrichment>
    <enrichment key="SourceTitle">Grebinyk, A., Prylutska, S., Grebinyk, S., Ponomarenko, S., Virych, P., Chumachenko, V., … Frohme, M. (Eds.). (2022). Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer. Nanoscale Advances, 4(23), 5077–5088.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1039/D2NA00353H</enrichment>
    <licence>Creative Commons - CC BY 3.0 - Namensnennung 3.0 Unported</licence>
    <author>Anna Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Sergii Grebinyk</author>
    <author>Stanislav Ponomarenko</author>
    <author>Pavlo Virych</author>
    <author>Vasyl Chumachenko</author>
    <author>Nataliya Kutsevol</author>
    <author>Yuriy Prylutskyy</author>
    <author>Uwe Ritter</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Funding" number="">Publikationsfonds der TH Wildau</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1674/d2na00353h.pdf</file>
  </doc>
</export-example>
