<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>49</id>
    <completedYear>2008</completedYear>
    <publishedYear>2008</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>35</pageFirst>
    <pageLast>39</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>13</volume>
    <type>articlewildau</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Genomic Analyses of Cryptobiotic Tardigrades</title>
    <abstract language="eng">Genomics technologies, especially transcriptional profiling, allows comparisons of gene expression within and across different organisms. By the use of the model group Tardigrada, also known as water bear, we try to understand the phenomenon of cryptobiosis. The dry organisms can survive for years without water. When re-exposed to water, the animals rehydrate and come back to life. The expression of genes in response to dehydratation and rehydratation is being examined in our laboratory through the generation of expressed sequence tags (ESTs), representational difference analysis (RDA) and subsequent microarray analysis. Molecular dissection of this complex phenomenom, including gene regulation, will allow the development of techniques for preservation and stabilisation of biological materials in a dried state.</abstract>
    <abstract language="deu">Techniken der Genomanalyse, speziell das transkriptionelle Profiling, erlauben uns, Genexpression innerhalb eines und zwischen verschiedenen Organismen zu untersuchen. Wir nutzen als Modell Tardigraden, auch bekannt als Bärtierchen, um das Phänomen der Kryptobiose besser zu verstehen. Die ausgetrockneten Tiere können Jahre überdauern. Wenn man Wasser hinzugibt, rehydrieren die Tiere und werden wieder zum Leben erweckt. In unserem Labor untersuchen wir die Genexpression als Antwort auf das Austrockenen und Rehydrieren mittels Bibliotheken Exprimierter Sequenz Tags (ESTs), Repräsentativer Differenz Analyse und Mikroarray-Untersuchungen. Die molekulare Analyse des komplexen Phänomens und seiner Regulation auf Ebene der Gene wird es ermöglichen, Techniken für die Konservierung und Stabilisierung von biologischem Material in trockenem Zustand zu entwickeln.</abstract>
    <parentTitle language="deu">Wissenschaftliche Beiträge 2008</parentTitle>
    <identifier type="issn">0949-8214</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus-672</identifier>
    <identifier type="opus3-id">67</identifier>
    <identifier type="doi">10.15771/0949-8214_2008_1_5</identifier>
    <enrichment key="InvalidVerification">123</enrichment>
    <enrichment key="ZSTiteliD">16238</enrichment>
    <licence>Creative Commons - CC BY-NC-ND 3.0 DE - Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschland</licence>
    <author>Brahim Mali</author>
    <author>Markus Grohme</author>
    <author>Weronika Wełnicz</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <author>Dirk Reuter</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="Publikationen_der_TH_Wildau" number="">Wissenschaftliche Beiträge</collection>
    <collection role="open_access" number="">open_access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/49/05_Mali_Frohme.pdf</file>
  </doc>
  <doc>
    <id>620</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>11</pageNumber>
    <edition/>
    <issue>168</issue>
    <volume>11</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradum in comparison with Hypsibius dujardini and Richtersius coronifer</title>
    <abstract language="eng">The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability of an organism to survive the loss of almost all cellular water without sustaining irreversible damage. Although there are several physiological, morphological and ecological studies on tardigrades, only limited DNA sequence information is available. Therefore, we explored the transcriptome in the active and anhydrobiotic state of the tardigrade Milnesium tardigradum which has extraordinary tolerance to desiccation and freezing. In this study, we present the first overview of the transcriptome of M. tardigradum and its response to desiccation and discuss potential parallels to stress responses in other organisms.</abstract>
    <parentTitle language="eng">BMC Genomics</parentTitle>
    <identifier type="issn">1471-2164</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6207</identifier>
    <enrichment key="SourceTitle">Mali et al.: Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradumin comparison with Hypsibius dujardini and Richtersius coronifer. BMC Genomics 2010 11:168.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/1471-2164-11-168</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Brahim Mali</author>
    <author>Markus Grohme</author>
    <author>Frank Förster</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <author>Dirk Reuter</author>
    <author>Weronika Wełnicz</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="576">Genetik und Evolution</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/620/2F1471-2164-11-168.pdf</file>
  </doc>
  <doc>
    <id>609</id>
    <completedYear>2013</completedYear>
    <publishedYear>2013</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>153</pageFirst>
    <pageLast>165</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>2013</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum</title>
    <abstract language="eng">Limno-terrestrial tardigrades are small invertebrates that are subjected to periodic drought of their micro-environment. They have evolved to cope with these unfavorable conditions by anhydrobiosis, an ametabolic state of low cellular water. During drying and rehydration, tardigrades go through drastic changes in cellular water content. By our transcriptome sequencing effort of the limno-terrestrial tardigrade Milnesium tardigradum and by a combination of cloning and targeted sequence assembly, we identified transcripts encoding eleven putative aquaporins. Analysis of these sequences proposed 2 classical aquaporins, 8 aquaglyceroporins and a single potentially intracellular unorthodox aquaporin. Using quantitative real-time PCR we analyzed aquaporin transcript expression in the anhydrobiotic context. We have identified additional unorthodox aquaporins in various insect genomes and have identified a novel common conserved structural feature in these proteins. Analysis of the genomic organization of insect aquaporin genes revealed several conserved gene clusters.</abstract>
    <parentTitle language="eng">Bioinformatics and Biology Insights</parentTitle>
    <identifier type="issn">1177-9322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6092</identifier>
    <enrichment key="SourceTitle">Grohme et al. The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum. Bioinformatics and Biology Insights 2013:7 153-165. DOI: 10.4137/BBI.S11497</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.4137/BBI.S11497</enrichment>
    <licence>Creative Commons - CC BY-NC 3.0 - Namensnennung-Nicht kommerziell 3.0 Unported</licence>
    <author>Markus Grohme</author>
    <author>Brahim Mali</author>
    <author>Weronika Wełnicz</author>
    <author>Stephanie Michel</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>unorthodox aquaporin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>anhydrobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/609/3696-BBI-The-Aquaporin-Channel-Repertoire-of-the-Tardigrade-Milnesium-tardigrad.pdf</file>
  </doc>
</export-example>
