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  <doc>
    <id>1470</id>
    <completedYear>2021</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>100293</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>24</volume>
    <type>article</type>
    <publisherName>Elsevier</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor</title>
    <abstract language="eng">Optical-resolution photoacoustic microscopy (OR-PAM) combines high spatial resolution and strong absorption-based contrast in tissue, which has enabled structural and spectroscopic imaging of endogenous chromophores, primarily hemoglobin. Conventional piezoelectric ultrasound transducers are typically placed far away from the photoacoustic source due to their opacity, which reduces acoustic sensitivity. Optical ultrasound sensors are an alternative as their transparency allows them to be positioned close to the sample with minimal source-detector distances. In this work, a backward-mode OR-PAM system based on a planar Fabry-Pérot ultrasound sensor and coaxially aligned excitation and interrogation beams was developed. Two 3D imaging modes, using raster-scanning for enhanced image quality and continuous-scanning for fast imaging, were implemented and tested on a leaf skeleton phantom. In fast imaging mode, a scan-rate of 100,000 A-lines/s was achieved. 3D images of a zebrafish embryo were acquired in vivo in raster-scanning mode. The transparency of the FP sensor in the visible and near-infrared wavelength region makes it suitable for combined functional and molecular imaging applications using OR-PAM and multi-photon fluorescence microscopy.</abstract>
    <parentTitle language="eng">Photoacoustics</parentTitle>
    <identifier type="issn">2213-5979</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-14701</identifier>
    <enrichment key="opus.import.date">2021-08-23T07:42:22+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.file">filename=php3kuEUb</enrichment>
    <enrichment key="opus.import.checksum">49d9fce876f7c09f19f7dab71f4c395c</enrichment>
    <enrichment key="SourceTitle">Baumann, E., Pohle, U., Zhang, E., Allen, T., Villringer, C., Pulwer, S., … Laufer, J. (2021). A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor. Photoacoustics, 24, 100293. doi:10.1016/j.pacs.2021.100293</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1016/j.pacs.2021.100293</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Elisabeth Baumann</author>
    <author>Ulrike Pohle</author>
    <author>Edward Zhang</author>
    <author>Thomas Allen</author>
    <author>Claus Villringer</author>
    <author>Silvio Pulwer</author>
    <author>Holger Gerhardt</author>
    <author>Jan Laufer</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>optical-resolution photoacoustic microscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>backward-mode imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>dual-wavelength</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>continuous scanning</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>in vivo imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>optical ultrasound sensing</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>planar Fabry-Pérot interferometer</value>
    </subject>
    <collection role="ddc" number="621">Angewandte Physik</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1470/1-s2.0-S2213597921000537-main.pdf</file>
  </doc>
  <doc>
    <id>1534</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>594</pageFirst>
    <pageLast>598</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>conferenceobject</type>
    <publisherName>SPIE</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Development of a backward-mode photoacoustic microscope using a Fabry-Pérot sensor</title>
    <abstract language="eng">Optical-resolution photoacoustic microscopy (PAM) has been shown to enable the acquisition of high resolution (μm) functional and anatomical images. For backward-mode operation, conventional piezoelectric ultrasound transducers need to be placed far away from the signal source due to their opacity and size. This can result in reduced acoustic sensitivity. Planar Fabry-Perot polymer film interferometer (FPI) sensors have the potential to overcome this limitation since they are transparent to the excitation wavelength, can be placed immediately adjacent to the signal source for high acoustic sensitivity, and offer a broadband frequency response (0 –50 MHz). In this study, we present a high frame rate, backward-mode OR-PAM system based on a planar FPI ultrasound sensor. A ns-pulsed laser provides excitation pulses (&lt;200 nJ, maximum pulse repetition frequency = 200 kHz, 532 nm) to generate photoacoustic waves that are detected using a planar FPI sensor interrogated at 765-781 nm. For backwardmode operation and highest acoustic sensitivity, the excitation and interrogation beams are coaxially aligned and rasterscanned. The optical transfer function of the sensor, the spatial resolution and the detection sensitivity were determined to characterise the set-up. Images of a leaf phantom and first in vivo images of zebrafish larvae were acquired. This approach will enable fast 3D OR-PAM with high resolution and high sensitivity for functional and molecular imaging applications. FPI-based ultrasound detection also has the potential to enable dual-mode optical- and acousticresolution PAM and the integration of photoacoustic imaging with purely optical modalities such as multi-photon microscopy.</abstract>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-15349</identifier>
    <enrichment key="opus.import.date">2021-09-17T11:06:23+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.file">filename=phpKmsZTn</enrichment>
    <enrichment key="opus.import.checksum">5ce6b8fda514c5661e139ec6556e337a</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1117/12.2525785</enrichment>
    <enrichment key="SourceTitle">Ulrike Pohle, Elisabeth Baumann, Silvio Pulwer, Claus Villringer, Edward Zhang, Holger Gerhardt, and Jan Laufer "Development of a backward-mode photoacoustic microscope using a Fabry-Pérot sensor", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108786L (6 March 2019); https://doi.org/10.1117/12.2525785</enrichment>
    <enrichment key="CopyrightInfo">Copyright 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Ulrike Pohle</author>
    <author>Elisabeth Baumann</author>
    <author>Silvio Pulwer</author>
    <author>Claus Villringer</author>
    <author>Edward Zhang</author>
    <author>Holger Gerhardt</author>
    <author>Jan Laufer</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>optical-resolution photoacoustic microscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fabry-Perot ultrasound sensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>in vivo imaging</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="2">Green Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1534/108786L.pdf</file>
  </doc>
</export-example>
