1687
2023
eng
2
24
article
MDPI
1
--
--
--
Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells
The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C60 fullerene (C60), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C60 effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C60 in HeLa cells reached its maximum at 24 h (800 ± 66 ng/106 cells). Half of extranuclear C60 is localized within mitochondria. The efficiency of the C60 nanostructure’s sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C60 was found for HeLa cells. C60′s ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment.
International Journal of Molecular Sciences
1422-0067
urn:nbn:de:kobv:526-opus4-16877
@Articleijms24021020, AUTHOR = Radivoievych, Aleksandar and Kolp, Benjamin and Grebinyk, Sergii and Prylutska, Svitlana and Ritter, Uwe and Zolk, Oliver and Glökler, Jörn and Frohme, Marcus and Grebinyk, Anna, TITLE = Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells, JOURNAL = International Journal of Molecular Sciences, VOLUME = 24, YEAR = 2023, NUMBER = 2, ARTICLE-NUMBER = 1020, URL = https://www.mdpi.com/1422-0067/24/2/1020, ISSN = 1422-0067, ABSTRACT = The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C60 fullerene (C60), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C60 effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C60 in HeLa cells reached its maximum at 24 h (800 ± 66 ng/106 cells). Half of extranuclear C60 is localized within mitochondria. The efficiency of the C60 nanostructure’s sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C60 was found for HeLa cells. C60′s ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment., DOI = 10.3390/ijms24021020
md5:76c5aa0796f241835d6d5c549fa910cb
2023-01-09T08:15:26+00:00
/tmp/phptq6JS9
bibtex
63bbcd1e4ffdd2.46312195
https://doi.org/10.3390/ijms24021020
Radivoievych, A.; Kolp, B.; Grebinyk, S.; Prylutska, S.; Ritter, U.; Zolk, O.; Glökler, J.; Frohme, M.; Grebinyk, A. Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells. Int. J. Mol. Sci. 2023, 24, 1020. https://doi.org/10.3390/ijms24021020
Creative Commons - CC BY - Namensnennung 4.0 International
Aleksandar Radivoievych
Benjamin Kolp
Sergii Grebinyk
Svitlana Prylutska
Uwe Ritter
Oliver Zolk
Jörn Glökler
Marcus Frohme
Anna Grebinyk
eng
uncontrolled
ultrasound
eng
uncontrolled
C60 fullerene
eng
uncontrolled
sonodynamic therapy
eng
uncontrolled
HeLa cells
eng
uncontrolled
apoptosis
Biowissenschaften; Biologie
Krankheiten
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Publikationsfonds der TH Wildau
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1687/ijms-24-01020.pdf
1036
2018
eng
2047
2052
19
article
1
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--
--
HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification
The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions.
This data article refers to the research article “C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al.
Data in Brief
10.1016/j.dib.2018.06.089
2352-3409
urn:nbn:de:kobv:526-opus4-10368
Grebinyk, A., Grebinyk, S., Prylutska, S., Ritter, U., Matyshevska, O., Dandekar, T., et al. (2018). HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification Data in Brief. 19, 2047-2052. https://doi.org/10.1016/j.dib.2018.06.089
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Sergii Grebinyk
Svitlana Prylutska
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
DFG-geförderter Publikationsfonds
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1036/1-s2.0-S2352340918307406-main.pdf
1058
2018
eng
319
327
124
article
1
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--
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C60 fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy
Recent progress in nanobiotechnology has attracted interest to a biomedical application of the carbon nanostructure C60 fullerene since it possesses a unique structure and versatile biological activity. C60 fullerene potential application in the frame of cancer photodynamic therapy (PDT) relies on rapid development of new light sources as well as on better understanding of the fullerene interaction with cells.
The aim of this study was to analyze C60 fullerene effects on human leukemic cells (CCRF-CEM) in combination with high power single chip light-emitting diodes (LEDs) light irradiation of different wavelengths: ultraviolet (UV, 365 nm), violet (405 nm), green (515 nm) and red (632 nm). The time-dependent accumulation of fullerene C60 in CCRF-CEM cells up to 250 ng/106 cells at 24 h with predominant localization within mitochondria was demonstrated with immunocytochemical staining and liquid chromatography mass spectrometry. In a cell viability assay we studied photoexcitation of the accumulated C60 nanostructures with ultraviolet or violet LEDs and could prove that significant phototoxic effects did arise. A less pronounced C60 fullerene phototoxic effect was observed after irradiation with green, and no effect was detected with red light. A C60 fullerene photoactivation with violet light induced substantial ROS generation and apoptotic cell death, confirmed by caspase3/7 activation and plasma membrane phosphatidylserine externalization. Our work proved C60 fullerene ability to induce apoptosis of leukemic cells after photoexcitation with high power single chip 405 nm LED as a light source. This underlined the potential for application of C60 nanostructure as a photosensitizer for anticancer therapy.
Free Radical Biology and Medicine
10.1016/j.freeradbiomed.2018.06.022
1873-4596
urn:nbn:de:kobv:526-opus4-10588
Grebinyk, A. et al. (2018). C60 fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy Free Radical Biology and Medicine. 124, 319-327.
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Sergii Grebinyk
Svitlana Prylutska
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Hybrid Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1058/1-s2.0-S0891584918311043-main.pdf
1477
2019
eng
conferenceobject
1
--
--
--
LED-based portable light source for photodynamic therapy
Photodynamic therapy (PDT) employs light activation of tissue-localized photosensitizer in an oxygen-dependent process which initiates oxidative stress, inflammation, and cell death. Laser systems, which are mostly used in PDT as light sources can be costly and oversized. light-emitting diodes (LEDs) equipment has a high potential to simplify technical part of phototriggered therapies and to reduce its costs. We develop the LED-based system that includes the control and irradiation units. The system provides the same power density at any irradiation point. Among the advantages of the device is a possibility to change the irradiation area and tune the irradiation dose. PDT experiments with cancer cells in vitro treated with two different photosensitizers demonstrated a possibility to use the developed LED-based system as a low-cost light source in PDT.
urn:nbn:de:kobv:526-opus4-14778
2021-08-26T11:19:53+00:00
sword
sword
filename=phpCgvAlh
c2d415e2348cf5f248f3ea95b8aac99c
https://doi.org/10.1117/12.2541774
O. Chepurna, A. Grebinyk, Yu. Petrushko, S. Prylutska, S. Grebinyk, V. M. Yashchuk, O. Matyshevska, U. Ritter, T. Dandekar, M. Frohme, J. Qu, and T. Y. Ohulchanskyy, "LED-based portable light source for photodynamic therapy", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111901A (20 November 2019); https://doi.org/10.1117/12.2541774
Copyright 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Das Dokument ist urheberrechtlich geschützt
Oksana Chepurna
Anna Grebinyk
Yulia Petrushko
Svitlana Prylutska
Sergii Grebinyk
Valeriy Yashchuk
Olga Matyshevska
Uwe Ritter
Thomas Dandekar
Marcus Frohme
Junle Qu
Tymish Y. Ohulchanskyy
eng
uncontrolled
light source
eng
uncontrolled
high power single chip LED
eng
uncontrolled
photodynamic therapy
Angewandte Physik
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Import
Green Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1477/111901A.pdf
1822
2023
eng
11
15
article
MDPI
1
--
--
--
Comparison of Sonodynamic Treatment Set-Ups for Cancer Cells with Organic Sonosensitizers and Nanosonosensitizers
Cancer sonodynamic therapy (SDT) is the therapeutic strategy of a high-frequency ultrasound (US) combined with a special sonosensitizer that becomes cytotoxic upon US exposure. The growing number of newly discovered sonosensitizers and custom US in vitro treatment solutions push the SDT field into a need for systemic studies and reproducible in vitro experimental set-ups. In the current research, we aimed to compare two of the most used and suitable SDT in vitro set-ups—“sealed well” and “transducer in well”—in one systematic study. We assessed US pressure, intensity, and temperature distribution in wells under US irradiation. Treatment efficacy was evaluated for both set-ups towards cancer cell lines of different origins, treated with two promising sonosensitizer candidates—carbon nanoparticle C60 fullerene (C60) and herbal alkaloid berberine. C60 was found to exhibit higher sonotoxicity toward cancer cells than berberine. The higher efficacy of sonodynamic treatment with a “transducer in well” set-up than a “sealed well” set-up underlined its promising application for SDT in vitro studies. The “transducer in well” set-up is recommended for in vitro US treatment investigations based on its US-field homogeneity and pronounced cellular effects. Moreover, SDT with C60 and berberine could be exploited as a promising combinative approach for cancer treatment.
Pharmaceutics
1999-4923
urn:nbn:de:kobv:526-opus4-18223
2023-11-13T08:02:06+00:00
sword
sword
https://doi.org/10.3390/pharmaceutics15112616
Radivoievych, A.; Prylutska, S.; Zolk, O.; Ritter, U.; Frohme, M.; Grebinyk, A. Comparison of Sonodynamic Treatment Set-Ups for Cancer Cells with Organic Sonosensitizers and Nanosonosensitizers. Pharmaceutics 2023, 15, 2616. https://doi.org/10.3390/pharmaceutics15112616
Creative Commons - CC BY - Namensnennung 4.0 International
Aleksandar Radivoievych
Svitlana Prylutska
Oliver Zolk
Uwe Ritter
Marcus Frohme
Anna Grebinyk
eng
uncontrolled
ultrasound
eng
uncontrolled
C60 fullerene
eng
uncontrolled
berberine
eng
uncontrolled
sonodynamic therapy
eng
uncontrolled
apoptosis
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Import
Publikationsfonds der TH Wildau
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1822/pharmaceutics-15-02616.pdf
1879
2024
eng
5
25
article
MDPI
1
--
--
--
Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro
The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology–Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated.
International Journal of Molecular Sciences
1422-0067
urn:nbn:de:kobv:526-opus4-18790
2024-03-06T13:41:20+00:00
sword
sword
https://doi.org/10.3390/ijms25053069
Prylutska, S.; Grebinyk, A.; Ponomarenko, S.; Gövem, D.; Chumachenko, V.; Kutsevol, N.; Petrovsky, M.; Ritter, U.; Frohme, M.; Piosik, J.; et al. Toxicity of Water-Soluble D-g-PNIPAM Polymers in a Complex with Chemotherapy Drugs and Mechanism of Their Action In Vitro. Int. J. Mol. Sci. 2024, 25, 3069. https://doi.org/10.3390/ijms25053069
false
true
Creative Commons - CC BY - Namensnennung 4.0 International
Svitlana Prylutska
Anna Grebinyk
Stanislav Ponomarenko
Defne Gövem
Vasyl Chumachenko
Nataliya Kutsevol
Mykola Petrovsky
Uwe Ritter
Marcus Frohme
Jacek Piosik
Yuriy Prylutskyy
eng
uncontrolled
star-like copolymer
eng
uncontrolled
drug
eng
uncontrolled
Lewis lung carcinoma
eng
uncontrolled
cytotoxicity
Organische Chemie
Pharmakologie, Therapeutik
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Import
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1879/ijms-25-03069.pdf
927
2017
eng
124
12
article
1
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--
--
Cytotoxic Effects of Dimorfolido-N-Trichloroacetylphosphorylamide and Dimorfolido-N-Benzoylphosphorylamide in Combination with C60 Fullerene on Leukemic Cells and Docking Study of Their Interaction with DNA
Dimorfolido-N-trichloroacetylphosphorylamide (HL1) and dimorfolido-N-benzoylphosphorylamide (HL2) as representatives of carbacylamidophosphates were synthesized and identified by the methods of IR, 1H, and 31P NMR spectroscopy. In vitro HL1 and HL2 at 1 mM concentration caused cell specific and time-dependent decrease of leukemic cell viability. Compounds caused the similar gradual decrease of Jurkat cells viability at 72 h (by 35%). HL1 had earlier and more profound toxic effect as compared to HL2 regardless on leukemic cell line. Viability of Molt-16 and CCRF-CEM cells under the action of HL1 was decreased at 24 h (by 32 and 45%, respectively) with no substantial further reducing up to 72 h. Toxic effect of HL2 was detected only at 72 h of incubation of Jurkat and Molt-16 cells (cell viability was decreased by 40 and 45%, respectively).
It was shown that C60 fullerene enhanced the toxic effect of HL2 on leukemic cells. Viability of Jurkat and CCRF-CEM cells at combined action of C60 fullerene and HL2 was decreased at 72 h (by 20 and 24%, respectively) in comparison with the effect of HL2 taken separately.
In silico study showed that HL1 and HL2 can interact with DNA and form complexes with DNA both separately and in combination with C60 fullerene. More stable complexes are formed when DNA interacts with HL1 or C60 + HL2 structure. Strong stacking interactions can be formed between HL2 and C60 fullerene. Differences in the types of identified bonds and ways of binding can determine distinction in cytotoxic effects of studied compounds.
Nanoscale Research Letters
1556-276X
10.1186/s11671-017-1893-3
urn:nbn:de:kobv:526-opus4-9273
Prylutska et al. Nanoscale Research Letters (2017) 12:124. DOI 10.1186/s11671-017-1893-3
Creative Commons - CC BY - Namensnennung 4.0 International
Svitlana Prylutska
I. Grynyuk
Anna Grebinyk
V. Hurmach
Iu. Shatrava
T. Sliva
V. Amirkhanov
Yuriy Prylutskyy
Olga Matyshevska
M. Slobodyanik
Marcus Frohme
Uwe Ritter
eng
uncontrolled
Dimorfolido-N-trichloroacetylphosphorylamide
eng
uncontrolled
Dimorfolido-N-benzoylphosphorylamide
eng
uncontrolled
C60 fullerene
eng
uncontrolled
leukemic cells
eng
uncontrolled
DNA
eng
uncontrolled
computer modeling
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/927/s11671-017-1893-3.pdf
1293
2019
eng
11
11
article
MDPI
1
--
2019-11-08
--
C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells
A herbal alkaloid Berberine (Ber), used for centuries in Ayurvedic, Chinese, Middle-Eastern, and native American folk medicines, is nowadays proved to function as a safe anticancer agent. Yet, its poor water solubility, stability, and bioavailability hinder clinical application. In this study, we have explored a nanosized carbon nanoparticle—C60 fullerene (C60)—for optimized Ber delivery into leukemic cells. Water dispersions of noncovalent C60-Ber nanocomplexes in the 1:2, 1:1, and 2:1 molar ratios were prepared. UV–Vis spectroscopy, dynamic light scattering (DLS), and atomic force microscopy (AFM) evidenced a complexation of the Ber cation with the negatively charged C60 molecule. The computer simulation showed that π-stacking dominates in Ber and C60 binding in an aqueous solution. Complexation with C60 was found to promote Ber intracellular uptake. By increasing C60 concentration, the C60-Ber nanocomplexes exhibited higher antiproliferative potential towards CCRF-CEM cells, in accordance with the following order: free Ber < 1:2 < 1:1 < 2:1 (the most toxic). The activation of caspase 3/7 and accumulation in the sub-G1 phase of CCRF-CEM cells treated with C60-Ber nanocomplexes evidenced apoptosis induction. Thus, this study indicates that the fast and easy noncovalent complexation of alkaloid Ber with C60 improved its in vitro efficiency against cancer cells.
Pharmaceutics
1999-4923
10.3390/pharmaceutics11110586
urn:nbn:de:kobv:526-opus4-12932
sword
2019-12-12T23:05:01+00:00
attachment; filename=deposit.zip
d3b76ebd17631c1dd7d3f3f0bd6f6ea4
Grebinyk, A., Prylutska, S., Buchelnikov, A., Tverdokhleb, N., Grebinyk, S., Evstigneev, M., et al. (2019). C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells Pharmaceutics. 11 (11), 586.
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Svitlana Prylutska
Anatoliy Buchelnikov
Nina Tverdokhleb
Sergii Grebinyk
Maxim Evstigneev
Olga Matyshevska
Vsevolod Cherepanov
Yuriy Prylutskyy
Valeriy Yashchuk
Anton Naumovets
Uwe Ritter
Thomas Dandekar
Marcus Frohme
eng
uncontrolled
C60 fullerene
eng
uncontrolled
Berberine
eng
uncontrolled
noncovalent nanocomplex
eng
uncontrolled
UV–Vis
eng
uncontrolled
DLS and AFM measurements
eng
uncontrolled
drug release
eng
uncontrolled
leukemic cells
eng
uncontrolled
uptake
eng
uncontrolled
cytotoxicity
eng
uncontrolled
apoptosis
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Import
DFG-geförderter Publikationsfonds
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1293/pharmaceutics-11-00586-v2.pdf
1294
2019
eng
11
9
article
MDPI
1
--
2019-10-30
--
Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex
A nanosized drug complex was explored to improve the efficiency of cancer chemotherapy, complementing it with nanodelivery and photodynamic therapy. For this, nanomolar amounts of a non-covalent nanocomplex of Doxorubicin (Dox) with carbon nanoparticle C60 fullerene (C60) were applied in 1:1 and 2:1 molar ratio, exploiting C60 both as a drug-carrier and as a photosensitizer. The fluorescence microscopy analysis of human leukemic CCRF-CEM cells, in vitro cancer model, treated with nanocomplexes showed Dox’s nuclear and C60’s extranuclear localization. It gave an opportunity to realize a double hit strategy against cancer cells based on Dox’s antiproliferative activity and C60’s photoinduced pro-oxidant activity. When cells were treated with 2:1 C60-Dox and irradiated at 405 nm the high cytotoxicity of photo-irradiated C60-Dox enabled a nanomolar concentration of Dox and C60 to efficiently kill cancer cells in vitro. The high pro-oxidant and pro-apoptotic efficiency decreased IC50 16, 9 and 7 × 103-fold, if compared with the action of Dox, non-irradiated nanocomplex, and C60’s photodynamic effect, correspondingly. Hereafter, a strong synergy of therapy arising from the combination of C60-mediated Dox delivery and C60 photoexcitation was revealed. Our data indicate that a combination of chemo- and photodynamic therapies with C60-Dox nanoformulation provides a promising synergetic approach for cancer treatment.
Nanomaterials
2079-4991
10.3390/nano9111540
urn:nbn:de:kobv:526-opus4-12940
sword
2019-12-13T01:52:58+00:00
attachment; filename=deposit.zip
f988bbe021d54d197e59d010cfa1917a
Grebinyk, A., Prylutska, S., Chepurna, O., Grebinyk, S., Prylutskyy, Y., Ritter, U., et al. (2019). Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex Nanomaterials. 9 (11), 1540.
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Svitlana Prylutska
Oksana Chepurna
Sergii Grebinyk
Yuriy Prylutskyy
Uwe Ritter
Tymish Y. Ohulchanskyy
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
eng
uncontrolled
photodynamic chemotherapy
eng
uncontrolled
synergistic effect
eng
uncontrolled
C60 fullerene
eng
uncontrolled
Doxorubicin
eng
uncontrolled
nanocomplex
eng
uncontrolled
leukemic cells
eng
uncontrolled
apoptosis
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Import
DFG-geförderter Publikationsfonds
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1294/nanomaterials-09-01540.pdf
1474
2021
eng
24
12
article
BioMed Central
1
--
--
--
Antitumor efficiency of the natural alkaloid berberine complexed with C60 fullerene in Lewis lung carcinoma in vitro and in vivo
Background
Berberine (Ber) is a herbal alkaloid with pharmacological activity in general and a high anticancer potency in particular. However, due to its low bioavailability, the difficulty in reaching a target and choosing the right dose, there is a need to improve approaches of Ber use in anticancer therapy. In this study, Ber, noncovalently bound to a carbon nanostructure C60 fullerene (C60) at various molar ratios of the components, was explored against Lewis lung carcinoma (LLC).
Methods
C60–Ber noncovalent nanocomplexes were synthesized in 1:2, 1:1 and 2:1 molar ratios. Ber release from the nanocomplexes was studied after prolonged incubation at different pH with the liquid chromatography–mass spectrometry analysis of free Ber content. Biological effects of the free and C60-complaxated Ber were studied in vitro towards LLC cells with phase-contrast and fluorescence microscopy, flow cytometry, MTT reduction, caspase activity and wound closure assays. The treatment with C60–Ber nanocomplex was evaluated in vivo with the LLC-tumored C57Bl mice. The mice body weight, tumor size, tumor weight and tumor weight index were assessed for four groups, treated with saline, 15 mg C60/kg, 7.5 mg Ber/kg or 2:1 C60-Ber nanocomplex (15 mg C60/kg, 7.5 mg Ber/kg).
Results
Ber release from C60–Ber nanocomplexes was promoted with medium acidification. LLC cells treatment with C60–Ber nanocomplexes was followed by enhanced Ber intracellular uptake as compared to free Ber. The cytotoxicity of the studied agents followed the order: free Ber < 1:2 < 1:1 < 2:1 C60–Ber nanocomplex. The potency of cytotoxic effect of 2:1 C60–Ber nanocomplex was confirmed by 21.3-fold decrease of IC50 value (0.8 ± 0.3 µM) compared to IC50 for free Ber (17 ± 2 µM). C60–Ber nanocomplexes induced caspase 3/7 activation and suppressed the migration activity of LLC cells. The therapeutic potency of 2:1 C60–Ber nanocomplex was confirmed in a mouse model of LLC. The tumor growth in the group treated with 2:1 C60–Ber nanocomplex is suppressed by approximately 50% at the end of experiment, while in the tumor-bearing group treated with free Ber no therapeutic effect was detected.
Conclusions
This study indicates that complexation of natural alkaloid Ber with C60 may be a novel therapeutic strategy against lung carcinoma.
Cancer Nanotechnology
1868-6966
urn:nbn:de:kobv:526-opus4-14749
publish
Grebinyk, A., Prylutska, S., Grebinyk, S. et al. Antitumor efficiency of the natural alkaloid berberine complexed with C60 fullerene in Lewis lung carcinoma in vitro and in vivo. Cancer Nano 12, 24 (2021). https://doi.org/10.1186/s12645-021-00096-6
https://doi.org/10.1186/s12645-021-00096-6
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Svitlana Prylutska
Sergii Grebinyk
Maxim Evstigneev
Iryna Krysiuk
Tetiana Skaterna
Iryna Horak
Yanfang Sun
Liudmyla Drobot
Olga Matyshevska
Yuriy Prylutskyy
Uwe Ritter
Marcus Frohme
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Projekt DEAL
Publikationsfonds der TH Wildau
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1474/s12645-021-00096-6.pdf
1674
2022
eng
5077
5088
23
4
article
Royal Society of Chemistry (RSC)
1
--
--
--
Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer
The development of precision cancer medicine relies on novel formulation strategies for targeted drug delivery to increase the therapeutic outcome. Biocompatible polymer nanoparticles, namely dextran-graft-polyacrylamide (D-g-PAA) copolymers, represent one of the innovative non-invasive approaches for drug delivery applications in cancer therapy. In this study, the star-like D-g-PAA copolymer in anionic form (D-g-PAAan) was developed for pH-triggered targeted drug delivery of the common chemotherapeutic drugs – doxorubicin (Dox) and cisplatin (Cis). The initial D-g-PAA copolymer was synthesized by the radical graft polymerization method, and then alkaline-hydrolyzed to get this polymer in anionic form for further use for drug encapsulation. The acidification of the buffer promoted the release of loaded drugs. D-g-PAAan nanoparticles increased the toxic potential of the drugs against human and mouse lung carcinoma cells (A549 and LLC), but not against normal human lung cells (HEL299). The drug-loaded D-g-PAAan-nanoparticles promoted further oxidative stress and apoptosis induction in LLC cells. D-g-PAAan-nanoparticles improved Dox accumulation and drugs’ toxicity in a 3D LLC multi-cellular spheroid model. The data obtained indicate that the strategy of chemotherapeutic drug encapsulation within the branched D-g-PAAan nanoparticle allows not only to realize pH-triggered drug release but also to potentiate its cytotoxic, prooxidant and proapoptotic effects against lung carcinoma cells.
Nanoscale Advances
urn:nbn:de:kobv:526-opus4-16749
@ArticleD2NA00353H, author ="Grebinyk, Anna and Prylutska, Svitlana and Grebinyk, Sergii and Ponomarenko, Stanislav and Virych, Pavlo and Chumachenko, Vasyl and Kutsevol, Nataliya and Prylutskyy, Yuriy and Ritter, Uwe and Frohme, Marcus", title ="Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer", journal ="Nanoscale Adv.", year ="2022", volume ="4", issue ="23", pages ="5077-5088", publisher ="RSC", doi ="10.1039/D2NA00353H", url ="http://dx.doi.org/10.1039/D2NA00353H", abstract ="The development of precision cancer medicine relies on novel formulation strategies for targeted drug delivery to increase the therapeutic outcome. Biocompatible polymer nanoparticles, namely dextran-graft-polyacrylamide (D-g-PAA) copolymers, represent one of the innovative non-invasive approaches for drug delivery applications in cancer therapy. In this study, the star-like D-g-PAA copolymer in anionic form (D-g-PAAan) was developed for pH-triggered targeted drug delivery of the common chemotherapeutic drugs – doxorubicin (Dox) and cisplatin (Cis). The initial D-g-PAA copolymer was synthesized by the radical graft polymerization method, and then alkaline-hydrolyzed to get this polymer in anionic form for further use for drug encapsulation. The acidification of the buffer promoted the release of loaded drugs. D-g-PAAan nanoparticles increased the toxic potential of the drugs against human and mouse lung carcinoma cells (A549 and LLC), but not against normal human lung cells (HEL299). The drug-loaded D-g-PAAan-nanoparticles promoted further oxidative stress and apoptosis induction in LLC cells. D-g-PAAan-nanoparticles improved Dox accumulation and drugs’ toxicity in a 3D LLC multi-cellular spheroid model. The data obtained indicate that the strategy of chemotherapeutic drug encapsulation within the branched D-g-PAAan nanoparticle allows not only to realize pH-triggered drug release but also to potentiate its cytotoxic, prooxidant and proapoptotic effects against lung carcinoma cells."
md5:f6a8c3c8e0076062674cfacef4031ed7
2022-11-25T11:39:42+00:00
/tmp/phpshl2n6
bibtex
6380a97e77fba6.10639762
Grebinyk, A., Prylutska, S., Grebinyk, S., Ponomarenko, S., Virych, P., Chumachenko, V., … Frohme, M. (Eds.). (2022). Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer. Nanoscale Advances, 4(23), 5077–5088.
https://doi.org/10.1039/D2NA00353H
Creative Commons - CC BY 3.0 - Namensnennung 3.0 Unported
Anna Grebinyk
Svitlana Prylutska
Sergii Grebinyk
Stanislav Ponomarenko
Pavlo Virych
Vasyl Chumachenko
Nataliya Kutsevol
Yuriy Prylutskyy
Uwe Ritter
Marcus Frohme
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Publikationsfonds der TH Wildau
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1674/d2na00353h.pdf
1096
2019
eng
14
article
1
--
--
--
Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro
Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.
Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function.
The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.
Nanoscale Research Letters
1556-276X
urn:nbn:de:kobv:526-opus4-10966
Grebinyk, A., Prylutska, S., Grebinyk, S. et al. Nanoscale Res Lett (2019) 14: 61. https://doi.org/10.1186/s11671-019-2894-1
https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-11415
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Svitlana Prylutska
Sergii Grebinyk
Yuriy Prylutskyy
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
eng
uncontrolled
C60 fullerene
eng
uncontrolled
Doxorubicin
eng
uncontrolled
Noncovalent complex
eng
uncontrolled
Leukemic cells
eng
uncontrolled
Cytotoxicity
eng
uncontrolled
Accumulation
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Gold Open Access
Technische Hochschule Wildau
1141
2019
eng
14
article
1
--
--
--
Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro
Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.
Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function.
The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.
Nanoscale Research Letters
1556-276X
10.1186/s11671-019-2894-1
urn:nbn:de:kobv:526-opus4-11415
Grebinyk, A., Prylutska, S., Grebinyk, S. et al. Nanoscale Res Lett (2019) 14: 61. https://doi.org/10.1186/s11671-019-2894-1
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Svitlana Prylutska
Sergii Grebinyk
Yuriy Prylutskyy
Uwe Ritter
Olga Matyshevska
Thomas Dandekar
Marcus Frohme
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
DFG-geförderter Publikationsfonds
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1141/2019_NRL_Grebinyk.pdf
1051
2018
eng
1
9
2018
article
1
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C60 Fullerene Effects on Diphenyl-N-(trichloroacetyl)-amidophosphate Interaction with DNA In Silico and Its Cytotoxic Activity Against Human Leukemic Cell Line In Vitro
New representative of carbacylamidophosphates - diphenyl-N-(trichloroacetyl)-amidophosphate (HL), which contains two phenoxy substituents near the phosphoryl group, was synthesized, identified by elemental analysis and IR and NMR spectroscopy, and tested as a cytotoxic agent itself and in combination with C60 fullerene.
According to molecular simulation results, C60 fullerene and HL could interact with DNA and form a rigid complex stabilized by stacking interactions of HL phenyl groups with C60 fullerene and DNA G nucleotide, as well as by interactions of HL CCl3 group by ion-π bonds with C60 molecule and by electrostatic bonds with DNA G nucleotide.
With the use of MTT test, the cytotoxic activity of HL against human leukemic CCRF-CM cells with IC50 value detected at 10 μM concentration at 72 h of cells treatment was shown. Under combined action of 16 μM C60 fullerene and HL, the value of IC50 was detected at lower 5 μM HL concentration and at earlier 48 h period of incubation, besides the cytotoxic effect of HL was observed at a low 2.5 μM concentration at which HL by itself had no influence on cell viability. Binding of C60 fullerene and HL with minor DNA groove with formation of a stable complex is assumed to be one of the possible reasons of their synergistic inhibition of CCRF-CЕM cells proliferation.
Application of C60 fullerene in combination with 2.5 μM HL was shown to have no harmful effect on structural stability of blood erythrocytes membrane. Thus, combined action of C60 fullerene and HL in a low concentration potentiated HL cytotoxic effect against human leukemic cells and was not followed by hemolytic effect.
Nanoscale Research Letters
10.1186/s11671-018-2490-9
1556-276X
urn:nbn:de:kobv:526-opus4-10515
Grebinyk, A. et al. (2018). C60 Fullerene Effects on Diphenyl-N-(trichloroacetyl)-amidophosphate Interaction with DNA In Silico and Its Cytotoxic Activity Against Human Leukemic Cell Line In Vitro Nanoscale Research Letters. 13 (1), 1-9. https://doi.org/10.1186/s11671-018-2490-9
Creative Commons - CC BY - Namensnennung 4.0 International
Anna Grebinyk
Svitlana Prylutska
I. Grynyuk
Benjamin Kolp
V. Hurmach
T. Sliva
V. Amirkhanov
V. Trush
Olga Matyshevska
M. Slobodyanik
Yuriy Prylutskyy
Marcus Frohme
Uwe Ritter
eng
uncontrolled
Diphenyl-N-(trichloroacetyl)-amidophosphate (HL)
eng
uncontrolled
C60 fullerene
eng
uncontrolled
Leukemic CCRF-CEM cells
eng
uncontrolled
DNA
eng
uncontrolled
Molecular simulation
Biowissenschaften; Biologie
Fachbereich Ingenieur- und Naturwissenschaften
open_access
Gold Open Access
Technische Hochschule Wildau
https://opus4.kobv.de/opus4-th-wildau/files/1051/s11671-018-2490-9.pdf