<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>1999</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>423</pageFirst>
    <pageLast>430</pageLast>
    <pageNumber/>
    <edition/>
    <issue>4</issue>
    <volume>15</volume>
    <type>article</type>
    <publisherName>Springer</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Stress response in tardigrades: differential gene expression of molecular chaperones</title>
    <abstract language="eng">Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (Mt-sHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.</abstract>
    <parentTitle language="eng">Cell Stress and Chaperones</parentTitle>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-19994</identifier>
    <enrichment key="opus.import.date">2025-02-13T07:52:45+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s12192-009-0158-1</enrichment>
    <enrichment key="SourceTitle">Reuner, A., Hengherr, S., Mali, B., Förster, F., Arndt, D., Reinhardt, R., … Schill, R. O. (2010). Stress response in tardigrades: differential gene expression of molecular chaperones. Cell Stress and Chaperones, 15(4), 423–430. doi:10.1007/s12192-009-0158-1</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>Andy Reuner</author>
    <author>Steffen Hengherr</author>
    <author>Brahim Mali</author>
    <author>Frank Förster</author>
    <author>Detlev Arndt</author>
    <author>Richard Reinhardt</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <author>Franz Brümmer</author>
    <author>Ralph O. Schill</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>alpha-crystallin protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>anhydrobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cryptobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>heat-shock protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Milnesium tardigradum</value>
    </subject>
    <collection role="ddc" number="571">Physiologie und verwandte Themen</collection>
    <collection role="ddc" number="592">Evertebrata (Wirbellose)</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1999/1-s2.0-S1355814523005679-main.pdf</file>
  </doc>
  <doc>
    <id>615</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>69</pageFirst>
    <pageLast>96</pageLast>
    <pageNumber/>
    <edition/>
    <issue>6</issue>
    <volume>2012</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations</title>
    <abstract language="eng">Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum.&#13;
To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions.&#13;
ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum.&#13;
These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant.</abstract>
    <parentTitle language="eng">Bioinformatics and Biology Insights</parentTitle>
    <identifier type="issn">1177-9322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6153</identifier>
    <enrichment key="SourceTitle">Förster et al. Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations. Bioinformatics and Biology Insights 2012:6 69-96</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.4137/BBI.S9150</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Frank Förster</author>
    <author>Daniela Beisser</author>
    <author>Markus Grohme</author>
    <author>Chunguang Liang</author>
    <author>Brahim Mali</author>
    <author>Alexander Matthias Siegl</author>
    <author>Julia C. Engelmann</author>
    <author>Alexander Shkumatov</author>
    <author>Elham Schokraie</author>
    <author>Tobias Müller</author>
    <author>Martina Schnölzer</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <author>Thomas Dandekar</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>RNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>expressed sequence tag</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cluster</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>protein family</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>adaptation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>transcriptome</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/615/3147-BBI-Transcriptome-Analysis-in-Tardigrade-Species-Reveals-Specific-Molecula.pdf</file>
  </doc>
  <doc>
    <id>609</id>
    <completedYear>2013</completedYear>
    <publishedYear>2013</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>153</pageFirst>
    <pageLast>165</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>2013</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum</title>
    <abstract language="eng">Limno-terrestrial tardigrades are small invertebrates that are subjected to periodic drought of their micro-environment. They have evolved to cope with these unfavorable conditions by anhydrobiosis, an ametabolic state of low cellular water. During drying and rehydration, tardigrades go through drastic changes in cellular water content. By our transcriptome sequencing effort of the limno-terrestrial tardigrade Milnesium tardigradum and by a combination of cloning and targeted sequence assembly, we identified transcripts encoding eleven putative aquaporins. Analysis of these sequences proposed 2 classical aquaporins, 8 aquaglyceroporins and a single potentially intracellular unorthodox aquaporin. Using quantitative real-time PCR we analyzed aquaporin transcript expression in the anhydrobiotic context. We have identified additional unorthodox aquaporins in various insect genomes and have identified a novel common conserved structural feature in these proteins. Analysis of the genomic organization of insect aquaporin genes revealed several conserved gene clusters.</abstract>
    <parentTitle language="eng">Bioinformatics and Biology Insights</parentTitle>
    <identifier type="issn">1177-9322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6092</identifier>
    <enrichment key="SourceTitle">Grohme et al. The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum. Bioinformatics and Biology Insights 2013:7 153-165. DOI: 10.4137/BBI.S11497</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.4137/BBI.S11497</enrichment>
    <licence>Creative Commons - CC BY-NC 3.0 - Namensnennung-Nicht kommerziell 3.0 Unported</licence>
    <author>Markus Grohme</author>
    <author>Brahim Mali</author>
    <author>Weronika Wełnicz</author>
    <author>Stephanie Michel</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>unorthodox aquaporin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>anhydrobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/609/3696-BBI-The-Aquaporin-Channel-Repertoire-of-the-Tardigrade-Milnesium-tardigrad.pdf</file>
  </doc>
</export-example>
