<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>582</id>
    <completedYear>2014</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>370</pageFirst>
    <pageLast>386</pageLast>
    <pageNumber/>
    <edition/>
    <issue>4</issue>
    <volume>4</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>2016-06-16</completedDate>
    <publishedDate>2014-10-22</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes</title>
    <abstract language="eng">Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently linked to the surface of MWCNTs while modification with p-phenylenediamine (PDA) converted the COOH-groups to positively charged amino groups. Fourier transform infrared spectroscopy (FTIR) measurements verified the p-phenylenediamine (PDA) modification of the polymer-CNT nanohybrids. The positively charged nanohybrid MWCNT-P(AMB-A)-PDA promoted direct electron transfer (DET) of CDH to the electrode and bioelectrocatalysis of lactose was observed. Amperometric measurements gave an electrochemical response with KMapp = 8.89 mM and a current density of 410 nA/cm2 (15 mM lactose). The catalytic response was tested at pH 3.5 and 4.5. Interference by ascorbic acid was not observed. The study proves that DET between the MWCNT-P(AMB-A)-PDA nanohybrids and CDH is efficient and allows the sensorial detection of lactose.</abstract>
    <parentTitle language="eng">Biosensors</parentTitle>
    <identifier type="issn">2079-6374</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-5826</identifier>
    <enrichment key="SourceTitle">Tanne, J.; Kracher, D.; Dietzel, B.; Schulz, B.; Ludwig, R.; Lisdat, F.; Scheller, F.W.; Bier, F.F.	Carboxylated or Aminated Polyaniline—Multiwalled Carbon Nanotubes Nanohybrids for Immobilization of Cellobiose Dehydrogenase on Gold Electrodes. Biosensors 2014, 4, 370-386.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.3390/bios4040370</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Johannes Tanne</author>
    <author>Daniel Kracher</author>
    <author>Birgit Dietzel</author>
    <author>Burkhard Schulz</author>
    <author>Roland Ludwig</author>
    <author>Fred Lisdat</author>
    <author>Frieder W. Scheller</author>
    <author>Frank Fabian Bier</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>multiwalled carbon nanotube</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>polyaniline</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>nanohybrids</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cellobiose dehydrogenase</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/582/bios4040370.pdf</file>
  </doc>
</export-example>
