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  <doc>
    <id>1999</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>423</pageFirst>
    <pageLast>430</pageLast>
    <pageNumber/>
    <edition/>
    <issue>4</issue>
    <volume>15</volume>
    <type>article</type>
    <publisherName>Springer</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Stress response in tardigrades: differential gene expression of molecular chaperones</title>
    <abstract language="eng">Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (Mt-sHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.</abstract>
    <parentTitle language="eng">Cell Stress and Chaperones</parentTitle>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-19994</identifier>
    <enrichment key="opus.import.date">2025-02-13T07:52:45+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s12192-009-0158-1</enrichment>
    <enrichment key="SourceTitle">Reuner, A., Hengherr, S., Mali, B., Förster, F., Arndt, D., Reinhardt, R., … Schill, R. O. (2010). Stress response in tardigrades: differential gene expression of molecular chaperones. Cell Stress and Chaperones, 15(4), 423–430. doi:10.1007/s12192-009-0158-1</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>Andy Reuner</author>
    <author>Steffen Hengherr</author>
    <author>Brahim Mali</author>
    <author>Frank Förster</author>
    <author>Detlev Arndt</author>
    <author>Richard Reinhardt</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <author>Franz Brümmer</author>
    <author>Ralph O. Schill</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>alpha-crystallin protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>anhydrobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cryptobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>heat-shock protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Milnesium tardigradum</value>
    </subject>
    <collection role="ddc" number="571">Physiologie und verwandte Themen</collection>
    <collection role="ddc" number="592">Evertebrata (Wirbellose)</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1999/1-s2.0-S1355814523005679-main.pdf</file>
  </doc>
  <doc>
    <id>49</id>
    <completedYear>2008</completedYear>
    <publishedYear>2008</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>35</pageFirst>
    <pageLast>39</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>13</volume>
    <type>articlewildau</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Genomic Analyses of Cryptobiotic Tardigrades</title>
    <abstract language="eng">Genomics technologies, especially transcriptional profiling, allows comparisons of gene expression within and across different organisms. By the use of the model group Tardigrada, also known as water bear, we try to understand the phenomenon of cryptobiosis. The dry organisms can survive for years without water. When re-exposed to water, the animals rehydrate and come back to life. The expression of genes in response to dehydratation and rehydratation is being examined in our laboratory through the generation of expressed sequence tags (ESTs), representational difference analysis (RDA) and subsequent microarray analysis. Molecular dissection of this complex phenomenom, including gene regulation, will allow the development of techniques for preservation and stabilisation of biological materials in a dried state.</abstract>
    <abstract language="deu">Techniken der Genomanalyse, speziell das transkriptionelle Profiling, erlauben uns, Genexpression innerhalb eines und zwischen verschiedenen Organismen zu untersuchen. Wir nutzen als Modell Tardigraden, auch bekannt als Bärtierchen, um das Phänomen der Kryptobiose besser zu verstehen. Die ausgetrockneten Tiere können Jahre überdauern. Wenn man Wasser hinzugibt, rehydrieren die Tiere und werden wieder zum Leben erweckt. In unserem Labor untersuchen wir die Genexpression als Antwort auf das Austrockenen und Rehydrieren mittels Bibliotheken Exprimierter Sequenz Tags (ESTs), Repräsentativer Differenz Analyse und Mikroarray-Untersuchungen. Die molekulare Analyse des komplexen Phänomens und seiner Regulation auf Ebene der Gene wird es ermöglichen, Techniken für die Konservierung und Stabilisierung von biologischem Material in trockenem Zustand zu entwickeln.</abstract>
    <parentTitle language="deu">Wissenschaftliche Beiträge 2008</parentTitle>
    <identifier type="issn">0949-8214</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus-672</identifier>
    <identifier type="opus3-id">67</identifier>
    <identifier type="doi">10.15771/0949-8214_2008_1_5</identifier>
    <enrichment key="InvalidVerification">123</enrichment>
    <enrichment key="ZSTiteliD">16238</enrichment>
    <licence>Creative Commons - CC BY-NC-ND 3.0 DE - Namensnennung - Nicht-kommerziell - Keine Bearbeitung 3.0 Deutschland</licence>
    <author>Brahim Mali</author>
    <author>Markus Grohme</author>
    <author>Weronika Wełnicz</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <author>Dirk Reuter</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="Publikationen_der_TH_Wildau" number="">Wissenschaftliche Beiträge</collection>
    <collection role="open_access" number="">open_access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/49/05_Mali_Frohme.pdf</file>
  </doc>
  <doc>
    <id>621</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>37</pageNumber>
    <edition/>
    <issue>3</issue>
    <volume>5</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Proteomic Analysis of Tardigrades: Towards a Better Understanding of Molecular Mechanisms by Anhydrobiotic Organisms</title>
    <abstract language="eng">Tardigrades are small, multicellular invertebrates which are able to survive times of unfavourable environmental conditions using their well-known capability to undergo cryptobiosis at any stage of their life cycle. Milnesium tardigradum has become a powerful model system for the analysis of cryptobiosis. While some genetic information is already available for Milnesium tardigradum the proteome is still to be discovered.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="issn">1932-6203</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6214</identifier>
    <enrichment key="SourceTitle">Schokraie E, Hotz-Wagenblatt A, Warnken U, Mali B, Frohme M, et al. (2010) Proteomic Analysis of Tardigrades: Towards a Better Understanding ofMolecular Mechanisms by Anhydrobiotic Organisms. PLoS ONE 5(3): e9502. doi:10.1371/journal.pone.0009502</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1371/journal.pone.0009502</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Elham Schokraie</author>
    <author>Agnes Hotz-Wagenblatt</author>
    <author>Uwe Warnken</author>
    <author>Brahim Mali</author>
    <author>Marcus Frohme</author>
    <author>Frank Förster</author>
    <author>Thomas Dandekar</author>
    <author>Steffen Hengherr</author>
    <author>Ralph O. Schill</author>
    <author>Martina Schnölzer</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/621/journal.pone.0009502.pdf</file>
  </doc>
  <doc>
    <id>639</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>25</pageNumber>
    <edition/>
    <issue>9</issue>
    <volume>7</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state</title>
    <abstract language="eng">Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="issn">1932-6203</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6392</identifier>
    <enrichment key="SourceTitle">Schokraie E, Warnken U, Hotz-Wagenblatt A, Grohme MA, Hengherr S, et al. (2012) Comparative proteome analysis ofMilnesium tardigradumin earlyembryonic stateversusadults in active and anhydrobiotic state. PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1371/journal.pone.0045682</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Elham Schokraie</author>
    <author>Uwe Warnken</author>
    <author>Agnes Hotz-Wagenblatt</author>
    <author>Markus Grohme</author>
    <author>Steffen Hengherr</author>
    <author>Frank Förster</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/639/journal.pone.0045682.pdf</file>
  </doc>
  <doc>
    <id>615</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>69</pageFirst>
    <pageLast>96</pageLast>
    <pageNumber/>
    <edition/>
    <issue>6</issue>
    <volume>2012</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations</title>
    <abstract language="eng">Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum.&#13;
To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions.&#13;
ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum.&#13;
These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant.</abstract>
    <parentTitle language="eng">Bioinformatics and Biology Insights</parentTitle>
    <identifier type="issn">1177-9322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6153</identifier>
    <enrichment key="SourceTitle">Förster et al. Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations. Bioinformatics and Biology Insights 2012:6 69-96</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.4137/BBI.S9150</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Frank Förster</author>
    <author>Daniela Beisser</author>
    <author>Markus Grohme</author>
    <author>Chunguang Liang</author>
    <author>Brahim Mali</author>
    <author>Alexander Matthias Siegl</author>
    <author>Julia C. Engelmann</author>
    <author>Alexander Shkumatov</author>
    <author>Elham Schokraie</author>
    <author>Tobias Müller</author>
    <author>Martina Schnölzer</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <author>Thomas Dandekar</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>RNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>expressed sequence tag</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cluster</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>protein family</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>adaptation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>transcriptome</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/615/3147-BBI-Transcriptome-Analysis-in-Tardigrade-Species-Reveals-Specific-Molecula.pdf</file>
  </doc>
  <doc>
    <id>646</id>
    <completedYear>2009</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>10</pageNumber>
    <edition/>
    <issue>469</issue>
    <volume>10</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Tardigrade workbench: comparing stress-related proteins, sequence-similar and functional protein clusters as well as RNA elements in tardigrades</title>
    <abstract language="eng">Background&#13;
Tardigrades represent an animal phylum with extraordinary resistance to environmental stress.&#13;
&#13;
Results&#13;
To gain insights into their stress-specific adaptation potential, major clusters of related and similar proteins are identified, as well as specific functional clusters delineated comparing all tardigrades and individual species (Milnesium tardigradum, Hypsibius dujardini, Echiniscus testudo, Tulinus stephaniae, Richtersius coronifer) and functional elements in tardigrade mRNAs are analysed. We find that 39.3% of the total sequences clustered in 58 clusters of more than 20 proteins. Among these are ten tardigrade specific as well as a number of stress-specific protein clusters. Tardigrade-specific functional adaptations include strong protein, DNA- and redox protection, maintenance and protein recycling. Specific regulatory elements regulate tardigrade mRNA stability such as lox P DICE elements whereas 14 other RNA elements of higher eukaryotes are not found. Further features of tardigrade specific adaption are rapidly identified by sequence and/or pattern search on the web-tool tardigrade analyzer http://waterbear.bioapps.biozentrum.uni-wuerzburg.de. The work-bench offers nucleotide pattern analysis for promotor and regulatory element detection (tardigrade specific; nrdb) as well as rapid COG search for function assignments including species-specific repositories of all analysed data.&#13;
&#13;
Conclusion&#13;
Different protein clusters and regulatory elements implicated in tardigrade stress adaptations are analysed including unpublished tardigrade sequences.</abstract>
    <parentTitle language="eng">BMC Genomics</parentTitle>
    <identifier type="issn">1471-2164</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6461</identifier>
    <enrichment key="SourceTitle">F. Förster et al. Tardigrade workbench: comparing stress-related proteins, sequence-similar and functional protein clusters as well as RNA elements in tardigrades. BMC Genomics 2009, 10:469</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/1471-2164-10-469</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Frank Förster</author>
    <author>Chunguang Liang</author>
    <author>Alexander Shkumatov</author>
    <author>Daniela Beisser</author>
    <author>Julia C. Engelmann</author>
    <author>Martina Schnölzer</author>
    <author>Marcus Frohme</author>
    <author>Tobias Müller</author>
    <author>Ralph O. Schill</author>
    <author>Thomas Dandekar</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/646/2F1471-2164-10-469.pdf</file>
  </doc>
  <doc>
    <id>620</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>11</pageNumber>
    <edition/>
    <issue>168</issue>
    <volume>11</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradum in comparison with Hypsibius dujardini and Richtersius coronifer</title>
    <abstract language="eng">The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability of an organism to survive the loss of almost all cellular water without sustaining irreversible damage. Although there are several physiological, morphological and ecological studies on tardigrades, only limited DNA sequence information is available. Therefore, we explored the transcriptome in the active and anhydrobiotic state of the tardigrade Milnesium tardigradum which has extraordinary tolerance to desiccation and freezing. In this study, we present the first overview of the transcriptome of M. tardigradum and its response to desiccation and discuss potential parallels to stress responses in other organisms.</abstract>
    <parentTitle language="eng">BMC Genomics</parentTitle>
    <identifier type="issn">1471-2164</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6207</identifier>
    <enrichment key="SourceTitle">Mali et al.: Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradumin comparison with Hypsibius dujardini and Richtersius coronifer. BMC Genomics 2010 11:168.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/1471-2164-11-168</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Brahim Mali</author>
    <author>Markus Grohme</author>
    <author>Frank Förster</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <author>Dirk Reuter</author>
    <author>Weronika Wełnicz</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="576">Genetik und Evolution</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/620/2F1471-2164-11-168.pdf</file>
  </doc>
  <doc>
    <id>602</id>
    <completedYear>2014</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>15</pageNumber>
    <edition/>
    <issue>3</issue>
    <volume>9</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>2014-03-20</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Towards Decrypting Cryptobiosis—Analyzing Anhydrobiosis in the Tardigrade Milnesium tardigradum Using Transcriptome Sequencing</title>
    <abstract language="eng">Many tardigrade species are capable of anhydrobiosis; however, mechanisms underlying their extreme desiccation resistance remain elusive. This study attempts to quantify the anhydrobiotic transcriptome of the limno-terrestrial tardigrade Milnesium tardigradum.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="issn">1932-6203</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6029</identifier>
    <enrichment key="SourceTitle">Wang C, Grohme MA, Mali B, Schill RO, Frohme M (2014) Towards Decrypting Cryptobiosis—Analyzing Anhydrobiosis in the Tardigrade Milnesium tardigradum Using Transcriptome Sequencing. PLoS ONE 9(3): e92663. doi:10.1371/journal.pone.0092663</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1371/journal.pone.0092663</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Chong Wang</author>
    <author>Markus Grohme</author>
    <author>Brahim Mali</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/602/journal.pone.0092663.pdf</file>
  </doc>
  <doc>
    <id>609</id>
    <completedYear>2013</completedYear>
    <publishedYear>2013</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>153</pageFirst>
    <pageLast>165</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>2013</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum</title>
    <abstract language="eng">Limno-terrestrial tardigrades are small invertebrates that are subjected to periodic drought of their micro-environment. They have evolved to cope with these unfavorable conditions by anhydrobiosis, an ametabolic state of low cellular water. During drying and rehydration, tardigrades go through drastic changes in cellular water content. By our transcriptome sequencing effort of the limno-terrestrial tardigrade Milnesium tardigradum and by a combination of cloning and targeted sequence assembly, we identified transcripts encoding eleven putative aquaporins. Analysis of these sequences proposed 2 classical aquaporins, 8 aquaglyceroporins and a single potentially intracellular unorthodox aquaporin. Using quantitative real-time PCR we analyzed aquaporin transcript expression in the anhydrobiotic context. We have identified additional unorthodox aquaporins in various insect genomes and have identified a novel common conserved structural feature in these proteins. Analysis of the genomic organization of insect aquaporin genes revealed several conserved gene clusters.</abstract>
    <parentTitle language="eng">Bioinformatics and Biology Insights</parentTitle>
    <identifier type="issn">1177-9322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6092</identifier>
    <enrichment key="SourceTitle">Grohme et al. The Aquaporin Channel Repertoire of the Tardigrade Milnesium tardigradum. Bioinformatics and Biology Insights 2013:7 153-165. DOI: 10.4137/BBI.S11497</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.4137/BBI.S11497</enrichment>
    <licence>Creative Commons - CC BY-NC 3.0 - Namensnennung-Nicht kommerziell 3.0 Unported</licence>
    <author>Markus Grohme</author>
    <author>Brahim Mali</author>
    <author>Weronika Wełnicz</author>
    <author>Stephanie Michel</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>unorthodox aquaporin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>anhydrobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/609/3696-BBI-The-Aquaporin-Channel-Repertoire-of-the-Tardigrade-Milnesium-tardigrad.pdf</file>
  </doc>
  <doc>
    <id>614</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>13</pageNumber>
    <edition/>
    <issue>72</issue>
    <volume>6</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Integrated pathway modules using time-course metabolic profiles and EST data from Milnesium tardigradum</title>
    <abstract language="eng">Tardigrades are multicellular organisms, resistant to extreme environmental changes such as heat, drought, radiation and freezing. They outlast these conditions in an inactive form (tun) to escape damage to cellular structures and cell death. Tardigrades are apparently able to prevent or repair such damage and are therefore a crucial model organism for stress tolerance. Cultures of the tardigrade Milnesium tardigradum were dehydrated by removing the surrounding water to induce tun formation. During this process and the subsequent rehydration, metabolites were measured in a time series by GC-MS. Additionally expressed sequence tags are available, especially libraries generated from the active and inactive state. The aim of this integrated analysis is to trace changes in tardigrade metabolism and identify pathways responsible for their extreme resistance against physical stress.</abstract>
    <parentTitle language="eng">BMC Systems Biology</parentTitle>
    <identifier type="issn">1752-0509</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6146</identifier>
    <enrichment key="SourceTitle">Beisser et al.:Integrated pathway modules using time-course metabolic profiles and EST data from Milnesium tardigradum. BMC Systems Biology 2012 6:72.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/1752-0509-6-72</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Daniela Beisser</author>
    <author>Markus Grohme</author>
    <author>Joachim Kopka</author>
    <author>Marcus Frohme</author>
    <author>Ralph O. Schill</author>
    <author>Steffen Hengherr</author>
    <author>Thomas Dandekar</author>
    <author>Gunnar W. Klau</author>
    <author>Marcus Dittrich</author>
    <author>Tobias Müller</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>integrated network analysis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>functional module</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>metabolic profile</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>metabolic pathway</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>trend test</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/614/2F1752-0509-6-72.pdf</file>
  </doc>
</export-example>
