<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>1999</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>423</pageFirst>
    <pageLast>430</pageLast>
    <pageNumber/>
    <edition/>
    <issue>4</issue>
    <volume>15</volume>
    <type>article</type>
    <publisherName>Springer</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Stress response in tardigrades: differential gene expression of molecular chaperones</title>
    <abstract language="eng">Semi-terrestrial tardigrades exhibit a remarkable tolerance to desiccation by entering a state called anhydrobiosis. In this state, they show a strong resistance against several kinds of physical extremes. Because of the probable importance of stress proteins during the phases of dehydration and rehydration, the relative abundance of transcripts coding for two α-crystallin heat-shock proteins (Mt-sHsp17.2 and Mt-sHsp19.5), as well for the heat-shock proteins Mt-sHsp10, Mt-Hsp60, Mt-Hsp70 and Mt-Hsp90, were analysed in active and anhydrobiotic tardigrades of the species Milnesium tardigradum. They were also analysed in the transitional stage (I) of dehydration, the transitional stage (II) of rehydration and in heat-shocked specimens. A variable pattern of expression was detected, with most candidates being downregulated. Gene transcripts of one Mt-hsp70 isoform in the transitional stage I and Mt-hsp90 in the anhydrobiotic stage were significantly upregulated. A high gene expression (778.6-fold) was found for the small α-crystallin heat-shock protein gene Mt-sHsp17.2 after heat shock. We discuss the limited role of the stress-gene expression in the transitional stages between the active and anhydrobiotic tardigrades and other mechanisms which allow tardigrades to survive desiccation.</abstract>
    <parentTitle language="eng">Cell Stress and Chaperones</parentTitle>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-19994</identifier>
    <enrichment key="opus.import.date">2025-02-13T07:52:45+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s12192-009-0158-1</enrichment>
    <enrichment key="SourceTitle">Reuner, A., Hengherr, S., Mali, B., Förster, F., Arndt, D., Reinhardt, R., … Schill, R. O. (2010). Stress response in tardigrades: differential gene expression of molecular chaperones. Cell Stress and Chaperones, 15(4), 423–430. doi:10.1007/s12192-009-0158-1</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>Andy Reuner</author>
    <author>Steffen Hengherr</author>
    <author>Brahim Mali</author>
    <author>Frank Förster</author>
    <author>Detlev Arndt</author>
    <author>Richard Reinhardt</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <author>Franz Brümmer</author>
    <author>Ralph O. Schill</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>alpha-crystallin protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>anhydrobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cryptobiosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>heat-shock protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Milnesium tardigradum</value>
    </subject>
    <collection role="ddc" number="571">Physiologie und verwandte Themen</collection>
    <collection role="ddc" number="592">Evertebrata (Wirbellose)</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1999/1-s2.0-S1355814523005679-main.pdf</file>
  </doc>
  <doc>
    <id>621</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>37</pageNumber>
    <edition/>
    <issue>3</issue>
    <volume>5</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Proteomic Analysis of Tardigrades: Towards a Better Understanding of Molecular Mechanisms by Anhydrobiotic Organisms</title>
    <abstract language="eng">Tardigrades are small, multicellular invertebrates which are able to survive times of unfavourable environmental conditions using their well-known capability to undergo cryptobiosis at any stage of their life cycle. Milnesium tardigradum has become a powerful model system for the analysis of cryptobiosis. While some genetic information is already available for Milnesium tardigradum the proteome is still to be discovered.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="issn">1932-6203</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6214</identifier>
    <enrichment key="SourceTitle">Schokraie E, Hotz-Wagenblatt A, Warnken U, Mali B, Frohme M, et al. (2010) Proteomic Analysis of Tardigrades: Towards a Better Understanding ofMolecular Mechanisms by Anhydrobiotic Organisms. PLoS ONE 5(3): e9502. doi:10.1371/journal.pone.0009502</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1371/journal.pone.0009502</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Elham Schokraie</author>
    <author>Agnes Hotz-Wagenblatt</author>
    <author>Uwe Warnken</author>
    <author>Brahim Mali</author>
    <author>Marcus Frohme</author>
    <author>Frank Förster</author>
    <author>Thomas Dandekar</author>
    <author>Steffen Hengherr</author>
    <author>Ralph O. Schill</author>
    <author>Martina Schnölzer</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/621/journal.pone.0009502.pdf</file>
  </doc>
  <doc>
    <id>639</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>25</pageNumber>
    <edition/>
    <issue>9</issue>
    <volume>7</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state</title>
    <abstract language="eng">Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="issn">1932-6203</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6392</identifier>
    <enrichment key="SourceTitle">Schokraie E, Warnken U, Hotz-Wagenblatt A, Grohme MA, Hengherr S, et al. (2012) Comparative proteome analysis ofMilnesium tardigradumin earlyembryonic stateversusadults in active and anhydrobiotic state. PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1371/journal.pone.0045682</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Elham Schokraie</author>
    <author>Uwe Warnken</author>
    <author>Agnes Hotz-Wagenblatt</author>
    <author>Markus Grohme</author>
    <author>Steffen Hengherr</author>
    <author>Frank Förster</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/639/journal.pone.0045682.pdf</file>
  </doc>
  <doc>
    <id>614</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>13</pageNumber>
    <edition/>
    <issue>72</issue>
    <volume>6</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Integrated pathway modules using time-course metabolic profiles and EST data from Milnesium tardigradum</title>
    <abstract language="eng">Tardigrades are multicellular organisms, resistant to extreme environmental changes such as heat, drought, radiation and freezing. They outlast these conditions in an inactive form (tun) to escape damage to cellular structures and cell death. Tardigrades are apparently able to prevent or repair such damage and are therefore a crucial model organism for stress tolerance. Cultures of the tardigrade Milnesium tardigradum were dehydrated by removing the surrounding water to induce tun formation. During this process and the subsequent rehydration, metabolites were measured in a time series by GC-MS. Additionally expressed sequence tags are available, especially libraries generated from the active and inactive state. The aim of this integrated analysis is to trace changes in tardigrade metabolism and identify pathways responsible for their extreme resistance against physical stress.</abstract>
    <parentTitle language="eng">BMC Systems Biology</parentTitle>
    <identifier type="issn">1752-0509</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6146</identifier>
    <enrichment key="SourceTitle">Beisser et al.:Integrated pathway modules using time-course metabolic profiles and EST data from Milnesium tardigradum. BMC Systems Biology 2012 6:72.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/1752-0509-6-72</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Daniela Beisser</author>
    <author>Markus Grohme</author>
    <author>Joachim Kopka</author>
    <author>Marcus Frohme</author>
    <author>Ralph O. Schill</author>
    <author>Steffen Hengherr</author>
    <author>Thomas Dandekar</author>
    <author>Gunnar W. Klau</author>
    <author>Marcus Dittrich</author>
    <author>Tobias Müller</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>integrated network analysis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>functional module</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>metabolic profile</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>metabolic pathway</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>trend test</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/614/2F1752-0509-6-72.pdf</file>
  </doc>
</export-example>
