<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>639</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>25</pageNumber>
    <edition/>
    <issue>9</issue>
    <volume>7</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state</title>
    <abstract language="eng">Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="issn">1932-6203</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6392</identifier>
    <enrichment key="SourceTitle">Schokraie E, Warnken U, Hotz-Wagenblatt A, Grohme MA, Hengherr S, et al. (2012) Comparative proteome analysis ofMilnesium tardigradumin earlyembryonic stateversusadults in active and anhydrobiotic state. PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1371/journal.pone.0045682</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Elham Schokraie</author>
    <author>Uwe Warnken</author>
    <author>Agnes Hotz-Wagenblatt</author>
    <author>Markus Grohme</author>
    <author>Steffen Hengherr</author>
    <author>Frank Förster</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/639/journal.pone.0045682.pdf</file>
  </doc>
  <doc>
    <id>615</id>
    <completedYear>2012</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>69</pageFirst>
    <pageLast>96</pageLast>
    <pageNumber/>
    <edition/>
    <issue>6</issue>
    <volume>2012</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations</title>
    <abstract language="eng">Tardigrades have unique stress-adaptations that allow them to survive extremes of cold, heat, radiation and vacuum.&#13;
To study this, encoded protein clusters and pathways from an ongoing transcriptome study on the tardigrade Milnesium tardigradum were analyzed using bioinformatics tools and compared to expressed sequence tags (ESTs) from Hypsibius dujardini, revealing major pathways involved in resistance against extreme environmental conditions.&#13;
ESTs are available on the Tardigrade Workbench along with software and databank updates. Our analysis reveals that RNA stability motifs for M. tardigradum are different from typical motifs known from higher animals. M. tardigradum and H. dujardini protein clusters and conserved domains imply metabolic storage pathways for glycogen, glycolipids and specific secondary metabolism as well as stress response pathways (including heat shock proteins, bmh2, and specific repair pathways). Redox-, DNA-, stress- and protein protection pathways complement specific repair capabilities to achieve the strong robustness of M. tardigradum.&#13;
These pathways are partly conserved in other animals and their manipulation could boost stress adaptation even in human cells. However, the unique combination of resistance and repair pathways make tardigrades and M. tardigradum in particular so highly stress resistant.</abstract>
    <parentTitle language="eng">Bioinformatics and Biology Insights</parentTitle>
    <identifier type="issn">1177-9322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6153</identifier>
    <enrichment key="SourceTitle">Förster et al. Transcriptome Analysis in Tardigrade Species Reveals Specific Molecular Pathways for Stress Adaptations. Bioinformatics and Biology Insights 2012:6 69-96</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.4137/BBI.S9150</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Frank Förster</author>
    <author>Daniela Beisser</author>
    <author>Markus Grohme</author>
    <author>Chunguang Liang</author>
    <author>Brahim Mali</author>
    <author>Alexander Matthias Siegl</author>
    <author>Julia C. Engelmann</author>
    <author>Alexander Shkumatov</author>
    <author>Elham Schokraie</author>
    <author>Tobias Müller</author>
    <author>Martina Schnölzer</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <author>Thomas Dandekar</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>RNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>expressed sequence tag</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cluster</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>protein family</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>adaptation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>tardigrada</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>transcriptome</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/615/3147-BBI-Transcriptome-Analysis-in-Tardigrade-Species-Reveals-Specific-Molecula.pdf</file>
  </doc>
  <doc>
    <id>620</id>
    <completedYear>2010</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>11</pageNumber>
    <edition/>
    <issue>168</issue>
    <volume>11</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradum in comparison with Hypsibius dujardini and Richtersius coronifer</title>
    <abstract language="eng">The phenomenon of desiccation tolerance, also called anhydrobiosis, involves the ability of an organism to survive the loss of almost all cellular water without sustaining irreversible damage. Although there are several physiological, morphological and ecological studies on tardigrades, only limited DNA sequence information is available. Therefore, we explored the transcriptome in the active and anhydrobiotic state of the tardigrade Milnesium tardigradum which has extraordinary tolerance to desiccation and freezing. In this study, we present the first overview of the transcriptome of M. tardigradum and its response to desiccation and discuss potential parallels to stress responses in other organisms.</abstract>
    <parentTitle language="eng">BMC Genomics</parentTitle>
    <identifier type="issn">1471-2164</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-6207</identifier>
    <enrichment key="SourceTitle">Mali et al.: Transcriptome survey of the anhydrobiotic tardigrade Milnesium tardigradumin comparison with Hypsibius dujardini and Richtersius coronifer. BMC Genomics 2010 11:168.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/1471-2164-11-168</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 2.0 Generic</licence>
    <author>Brahim Mali</author>
    <author>Markus Grohme</author>
    <author>Frank Förster</author>
    <author>Thomas Dandekar</author>
    <author>Martina Schnölzer</author>
    <author>Dirk Reuter</author>
    <author>Weronika Wełnicz</author>
    <author>Ralph O. Schill</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="576">Genetik und Evolution</collection>
    <collection role="institutes" number="">Fachbereich Ingenieurwesen / Wirtschaftsingenieurwesen (bis 8/2014)</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/620/2F1471-2164-11-168.pdf</file>
  </doc>
</export-example>
