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  <doc>
    <id>1482</id>
    <completedYear>2015</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>conferenceobject</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles</title>
    <abstract language="eng">In the present study we report on the ability of gold nanoparticles (AuNP) to enhance the antimicrobial activity of the photosensitizer Rose Bengal (RB), a very effective singlet oxygen generator. Our experiments were conducted using a suspension of Escherichia Coli in the presence of either RB or a combination of RB and AuNP. Nanoparticles were synthesized by laser ablation in water, which allows high purity, biologically friendly AuNP production, as compared to traditional chemical methods. Several relative concentrations of bacteria, photosensitizes and AuNP were studied. Bacterial survival rates were determined before and after LED light illumination. The phototoxicity of RB with and without AuNP was checked following illumination for 10 and 20 minutes. As a control, the dark toxicity of RB was verified. The results show that the survival rate of bacteria decreases significantly with the increase of RB concentration and illumination time, which is in accordance with previous works. Interestingly, our results also indicate a significant increase in the lethal photosensitization of RB in the presence of AuNP. We propose this effect is due to plasmonic light enhancement, considering the superposition of RB and AuNP absorption spectra, which favors electric field enhancement effects in the presence of AuNP. Similar experiments using the photosensitizer Methylene Blue (MB) allowed us to test our hypothesis for MB did not show any difference in its phototoxicity in the presence of AuNP. We propose this observed synergistic effect could be an effective way for improving photodynamic inactivation of microorganisms.</abstract>
    <parentTitle language="eng">Proc. SPIE 9531, Biophotonics South America, 953138 (19 June 2015)</parentTitle>
    <identifier type="issn">1996-756X</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-14828</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="SourceTitle">Heike Kagel, Julia Honselmann Genannt Humme, Edvaldo Antonio Ribeiro Rosa, Rozane de Fátima Turchiello, and Arandi Ginane Bezerra Junior "Plasmonic enhancement in the photoinactivation of Escherichia Coli using rose bengal and gold nanoparticles", Proc. SPIE 9531, Biophotonics South America, 953138 (19 June 2015); https://doi.org/10.1117/12.2180947</enrichment>
    <enrichment key="CopyrightInfo">Copyright (2015) Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1117/12.2180947</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Heike Kagel</author>
    <author>Julia Honselmann Genannt Humme</author>
    <author>Edvaldo Antonio Ribeiro Rosa</author>
    <author>Rozane de Fátima Turchiello</author>
    <author>Arandi Ginane Bezerra Junior</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="2">Green Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1482/953138.pdf</file>
  </doc>
</export-example>
