<?xml version="1.0" encoding="utf-8"?>
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  <doc>
    <id>1053</id>
    <completedYear>2018</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>12</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>10</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes</title>
    <abstract language="eng">Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.</abstract>
    <parentTitle language="eng">Genome Medicine</parentTitle>
    <identifier type="issn">1756-994X</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-10538</identifier>
    <enrichment key="SourceTitle">Devulapally, P., Buerger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M., et al. (2018). Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes Genome Medicine. 10, 1-12. https://doi.org/10.1186/s13073-018-0542-5</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/s13073-018-0542-5</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Praneeth Reddy Devulapally</author>
    <author>Jörg Bürger</author>
    <author>Thorsten Mielke</author>
    <author>Zoltán Konthur</author>
    <author>Hans Lehrach</author>
    <author>Marie-Laure Yaspo</author>
    <author>Jörn Glökler</author>
    <author>Hans-Jörg Warnatz</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1053/s13073-018-0542-5.pdf</file>
  </doc>
</export-example>
