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  <doc>
    <id>1612</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>23</pageFirst>
    <pageLast>30</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1-2</issue>
    <volume>4</volume>
    <type>article</type>
    <publisherName>IOS Press</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Photoacids in biochemical applications</title>
    <abstract language="eng">BACKGROUND:&#13;
After excitation with light photoacids can change the pH in a solution by release of a proton. They have been used mostly for excited state proton transfer studies. In this review the general functionality and mechanisms and the subdivision of photoacids is explained.&#13;
&#13;
STATE OF THE ART:&#13;
Different uses of photoacids are described, covering a wide range of various biochemical topics, focusing on biochemical applications. Examples for the introduced subdivisions are covered.&#13;
&#13;
CONCLUSIONS AND OUTLOOK:&#13;
The areas in which photoacids can be employed are diverse. Photoacids have a promising future in biotechnology and biochemistry and should be considered for upcoming applications, especially in non-invasive control of biochemical reactions.</abstract>
    <parentTitle language="eng">Journal of Cellular Biotechnology</parentTitle>
    <identifier type="issn">2352-3697</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-16123</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.3233/JCB-189004</enrichment>
    <enrichment key="SourceTitle">Kagel H, Frohme M, Glökler J. Photoacids in biochemical applications. Journal of Cellular Biotechnology. 2019;4(1-2):23-30. doi:10.3233/JCB-189004</enrichment>
    <licence>Creative Commons - CC BY-NC - Namensnennung - Nicht kommerziell 4.0 International</licence>
    <author>Heike Kagel</author>
    <author>Marcus Frohme</author>
    <author>Jörn Glökler</author>
    <collection role="ddc" number="572">Biochemie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1612/189004.pdf</file>
  </doc>
  <doc>
    <id>1610</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>945</pageFirst>
    <pageLast>956</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>9</volume>
    <type>article</type>
    <publisherName>Springer</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>2019-07-01</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A new triple system DNA-Nanosilver-Berberine for cancer therapy</title>
    <abstract language="eng">The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA. In the current work, we have investigated the effects of Berberine on the human T cell leukemia cell line in vitro. Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time- and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction. Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers. The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanoparticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment.</abstract>
    <parentTitle language="eng">Applied Nanoscience</parentTitle>
    <identifier type="issn">2190-5517</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-16105</identifier>
    <enrichment key="opus.import.date">2022-05-12T12:02:28+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
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    <enrichment key="SourceTitle">Grebinyk, A., Yashchuk, V., Bashmakova, N. et al. A new triple system DNA-Nanosilver-Berberine for cancer therapy. Appl Nanosci 9, 945–956 (2019). https://doi.org/10.1007/s13204-018-0688-x</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s13204-018-0688-x</enrichment>
    <enrichment key="RelatedIdentifier">https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/1062</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Valeriy Yashchuk</author>
    <author>Nataliya Bashmakova</author>
    <author>Dmytro Gryn</author>
    <author>Tobias Hagemann</author>
    <author>Antonina Naumenko</author>
    <author>Nataliya Kutsevol</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1610/Grebinyk2019_Article.pdf</file>
  </doc>
  <doc>
    <id>1477</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>conferenceobject</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">LED-based portable light source for photodynamic therapy</title>
    <abstract language="eng">Photodynamic therapy (PDT) employs light activation of tissue-localized photosensitizer in an oxygen-dependent process which initiates oxidative stress, inflammation, and cell death. Laser systems, which are mostly used in PDT as light sources can be costly and oversized. light-emitting diodes (LEDs) equipment has a high potential to simplify technical part of phototriggered therapies and to reduce its costs. We develop the LED-based system that includes the control and irradiation units. The system provides the same power density at any irradiation point. Among the advantages of the device is a possibility to change the irradiation area and tune the irradiation dose. PDT experiments with cancer cells in vitro treated with two different photosensitizers demonstrated a possibility to use the developed LED-based system as a low-cost light source in PDT.</abstract>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-14778</identifier>
    <enrichment key="opus.import.date">2021-08-26T11:19:53+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.file">filename=phpCgvAlh</enrichment>
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    <enrichment key="DOI_VoR">https://doi.org/10.1117/12.2541774</enrichment>
    <enrichment key="SourceTitle">O. Chepurna, A. Grebinyk, Yu. Petrushko, S. Prylutska, S. Grebinyk, V. M. Yashchuk, O. Matyshevska, U. Ritter, T. Dandekar, M. Frohme, J. Qu, and T. Y. Ohulchanskyy, "LED-based portable light source for photodynamic therapy", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111901A (20 November 2019); https://doi.org/10.1117/12.2541774</enrichment>
    <enrichment key="CopyrightInfo">Copyright 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Oksana Chepurna</author>
    <author>Anna Grebinyk</author>
    <author>Yulia Petrushko</author>
    <author>Svitlana Prylutska</author>
    <author>Sergii Grebinyk</author>
    <author>Valeriy Yashchuk</author>
    <author>Olga Matyshevska</author>
    <author>Uwe Ritter</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <author>Junle Qu</author>
    <author>Tymish Y. Ohulchanskyy</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>light source</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>high power single chip LED</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>photodynamic therapy</value>
    </subject>
    <collection role="ddc" number="621">Angewandte Physik</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="2">Green Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1477/111901A.pdf</file>
  </doc>
  <doc>
    <id>1294</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>11</issue>
    <volume>9</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>2019-10-30</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex</title>
    <abstract language="eng">A nanosized drug complex was explored to improve the efficiency of cancer chemotherapy, complementing it with nanodelivery and photodynamic therapy. For this, nanomolar amounts of a non-covalent nanocomplex of Doxorubicin (Dox) with carbon nanoparticle C60 fullerene (C60) were applied in 1:1 and 2:1 molar ratio, exploiting C60 both as a drug-carrier and as a photosensitizer. The fluorescence microscopy analysis of human leukemic CCRF-CEM cells, in vitro cancer model, treated with nanocomplexes showed Dox’s nuclear and C60’s extranuclear localization. It gave an opportunity to realize a double hit strategy against cancer cells based on Dox’s antiproliferative activity and C60’s photoinduced pro-oxidant activity. When cells were treated with 2:1 C60-Dox and irradiated at 405 nm the high cytotoxicity of photo-irradiated C60-Dox enabled a nanomolar concentration of Dox and C60 to efficiently kill cancer cells in vitro. The high pro-oxidant and pro-apoptotic efficiency decreased IC50 16, 9 and 7 × 103-fold, if compared with the action of Dox, non-irradiated nanocomplex, and C60’s photodynamic effect, correspondingly. Hereafter, a strong synergy of therapy arising from the combination of C60-mediated Dox delivery and C60 photoexcitation was revealed. Our data indicate that a combination of chemo- and photodynamic therapies with C60-Dox nanoformulation provides a promising synergetic approach for cancer treatment.</abstract>
    <parentTitle language="eng">Nanomaterials</parentTitle>
    <identifier type="issn">2079-4991</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-12940</identifier>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.date">2019-12-13T01:52:58+00:00</enrichment>
    <enrichment key="opus.import.file">attachment; filename=deposit.zip</enrichment>
    <enrichment key="opus.import.checksum">f988bbe021d54d197e59d010cfa1917a</enrichment>
    <enrichment key="SourceTitle">Grebinyk, A., Prylutska, S., Chepurna, O., Grebinyk, S., Prylutskyy, Y., Ritter, U., et al. (2019). Synergy of Chemo- and Photodynamic Therapies with C60 Fullerene-Doxorubicin Nanocomplex Nanomaterials. 9 (11), 1540.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.3390/nano9111540</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Oksana Chepurna</author>
    <author>Sergii Grebinyk</author>
    <author>Yuriy Prylutskyy</author>
    <author>Uwe Ritter</author>
    <author>Tymish Y. Ohulchanskyy</author>
    <author>Olga Matyshevska</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>photodynamic chemotherapy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>synergistic effect</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>C60 fullerene</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>doxorubicin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>nanocomplex</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>leukemic cell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>apoptosis</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="Funding" number="">DFG-geförderter Publikationsfonds</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1294/nanomaterials-09-01540.pdf</file>
  </doc>
  <doc>
    <id>1293</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>11</issue>
    <volume>11</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>2019-11-08</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells</title>
    <abstract language="eng">A herbal alkaloid Berberine (Ber), used for centuries in Ayurvedic, Chinese, Middle-Eastern, and native American folk medicines, is nowadays proved to function as a safe anticancer agent. Yet, its poor water solubility, stability, and bioavailability hinder clinical application. In this study, we have explored a nanosized carbon nanoparticle—C60 fullerene (C60)—for optimized Ber delivery into leukemic cells. Water dispersions of noncovalent C60-Ber nanocomplexes in the 1:2, 1:1, and 2:1 molar ratios were prepared. UV–Vis spectroscopy, dynamic light scattering (DLS), and atomic force microscopy (AFM) evidenced a complexation of the Ber cation with the negatively charged C60 molecule. The computer simulation showed that π-stacking dominates in Ber and C60 binding in an aqueous solution. Complexation with C60 was found to promote Ber intracellular uptake. By increasing C60 concentration, the C60-Ber nanocomplexes exhibited higher antiproliferative potential towards CCRF-CEM cells, in accordance with the following order: free Ber &lt; 1:2 &lt; 1:1 &lt; 2:1 (the most toxic). The activation of caspase 3/7 and accumulation in the sub-G1 phase of CCRF-CEM cells treated with C60-Ber nanocomplexes evidenced apoptosis induction. Thus, this study indicates that the fast and easy noncovalent complexation of alkaloid Ber with C60 improved its in vitro efficiency against cancer cells.</abstract>
    <parentTitle language="eng">Pharmaceutics</parentTitle>
    <identifier type="issn">1999-4923</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-12932</identifier>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.date">2019-12-12T23:05:01+00:00</enrichment>
    <enrichment key="opus.import.file">attachment; filename=deposit.zip</enrichment>
    <enrichment key="opus.import.checksum">d3b76ebd17631c1dd7d3f3f0bd6f6ea4</enrichment>
    <enrichment key="SourceTitle">Grebinyk, A., Prylutska, S., Buchelnikov, A., Tverdokhleb, N., Grebinyk, S., Evstigneev, M., et al. (2019). C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells Pharmaceutics. 11 (11), 586.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.3390/pharmaceutics11110586</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Anatoliy Buchelnikov</author>
    <author>Nina Tverdokhleb</author>
    <author>Sergii Grebinyk</author>
    <author>Maxim Evstigneev</author>
    <author>Olga Matyshevska</author>
    <author>Vsevolod Cherepanov</author>
    <author>Yuriy Prylutskyy</author>
    <author>Valeriy Yashchuk</author>
    <author>Anton Naumovets</author>
    <author>Uwe Ritter</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>C60 fullerene</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>berberine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>noncovalent nanocomplex</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>UV–Vis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DLS and AFM measurements</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>drug release</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>leukemic cell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>uptake</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cytotoxicity</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>apoptosis</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="Funding" number="">DFG-geförderter Publikationsfonds</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1293/pharmaceutics-11-00586-v2.pdf</file>
  </doc>
  <doc>
    <id>1268</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>9</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids</title>
    <abstract language="eng">Most biochemical reactions depend on the pH value of the aqueous environment and some are strongly favoured to occur in an acidic environment. A non-invasive control of pH to tightly regulate such reactions with defined start and end points is a highly desirable feature in certain applications, but has proven difficult to achieve so far. We report a novel optical approach to reversibly control a typical biochemical reaction by changing the pH and using acid phosphatase as a model enzyme. The reversible photoacid G-acid functions as a proton donor, changing the pH rapidly and reversibly by using high power UV LEDs as an illumination source in our experimental setup. The reaction can be tightly controlled by simply switching the light on and off and should be applicable to a wide range of other enzymatic reactions, thus enabling miniaturization and parallelization through non-invasive optical means.</abstract>
    <parentTitle language="eng">Scientific Reports</parentTitle>
    <identifier type="issn">2045-2322</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-12685</identifier>
    <enrichment key="SourceTitle">Kagel, H., Bier, F., Frohme, M., &amp; Glökler, J. (2019). A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids Scientific Reports. 9, 14372 (2019). DOI: 10.1038/s41598-019-50867-w</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1038/s41598-019-50867-w</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Heike Kagel</author>
    <author>Frank Fabian Bier</author>
    <author>Marcus Frohme</author>
    <author>Jörn Glökler</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Funding" number="">DFG-geförderter Publikationsfonds</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1268/s41598-019-50867-w.pdf</file>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1268/41598_2019_50867_MOESM1_ESM.pdf</file>
  </doc>
  <doc>
    <id>1238</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>5993</pageFirst>
    <pageLast>6003</pageLast>
    <pageNumber/>
    <edition/>
    <issue>23</issue>
    <volume>411</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Quantification of nitroaromatic explosives in contaminated soil using MALDI-TOF mass spectrometry</title>
    <abstract language="eng">Contamination from various sources is a global environmental and health threat, with mining and military activities in particular having spread nitroaromatic compounds, such as 2,4,6-trinitrotoluene and its degradation products and by-products, to the soil. The investigation and monitoring of large contaminated areas requires new detection methods since the established ones are expensive and time-consuming. Hence, we established a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI--TOF MS) method using 1,5-diaminonaphthalene as the matrix substance and an internal standard for quantification. Analyzing standard substances, we found specific signals for radical and fragment ions of different nitrotoluenes and nitrobenzenes with good reproducibility and detection limits down to 0.25 ng/μL. The analysis of soil sample extracts from a former production site showed clear signals for 2,4,6-trinitrotoluene and the primary degradation products aminodinitrotoluenes. Furthermore, quantification gave results comparable to those obtained by conventional liquid chromatography--tandem mass spectrometry analysis. The MALDI-TOF MS method has a comparatively lower reproducibility, with relative standard deviations of 6% to 20% for multiple measurements of standard solutions and soil sample extracts. Nevertheless, a comparison of both methods revealed the advantages of MALDI-TOF MS analysis of explosive-contaminated areas with regard to costs, time, and handling. Finally, our MALDI-TOF MS method fulfills all the needs for high sample throughput and can therefore be a valuable screening tool for explosive-contaminated areas.</abstract>
    <parentTitle language="eng">Analytical and Bioanalytical Chemistry</parentTitle>
    <identifier type="issn">1618-2650</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-12388</identifier>
    <enrichment key="opus.import.user">sword</enrichment>
    <enrichment key="opus.import.date">2019-07-09T11:39:29+00:00</enrichment>
    <enrichment key="opus.import.file">filename=phpd4tZMy</enrichment>
    <enrichment key="opus.import.checksum">35d67665c04d71330c075096977d1cae</enrichment>
    <enrichment key="SourceTitle">Kober, S.L., Hollert, H. &amp; Frohme, M. Anal Bioanal Chem (2019) 411: 5993. https://doi.org/10.1007/s00216-019-01976-y</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s00216-019-01976-y</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Liane Kober</author>
    <author>Henner Hollert</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>explosives</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>soil contamination</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TNT</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>matrix-assisted laser desorption/ionization quantification</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Import" number="import">Import</collection>
    <collection role="green_open_access" number="4">Hybrid Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1238/Kober2019_Article_QuantificationOfNitroaromaticE.pdf</file>
  </doc>
  <doc>
    <id>1201</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>20</pageNumber>
    <edition/>
    <issue/>
    <volume/>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Re-Emerging Foci of Visceral Leishmaniasis in Armenia – First Molecular Diagnosis of Clinical Samples</title>
    <abstract language="eng">Visceral leishmaniasis (VL) was firstly reported in Armenia in 1913. Following a considerable increase of the number of cases until the mid 1950s, the diseases disappeared after 1969 and re-emerged in 1999. Scientific literature about VL in Armenia  is available only in Russian or Armenian. This paper presents a historical overview about leishmaniasis in Armenia based on this literature as well as an epidemiological update since the re-emergence of the disease. In 1999- 2016, 116 indigenous VL cases were recorded mainly in children in 8 of the 11 districts, however VL is underreported because of lack of trained medical personal and diagnostic facilities. The aim of this work was to apply for the first time molecular diagnosis of VL in Armenia. Out of 25 VL suspected patients, 22 were positive by microscopy and PCR. Genotyping using ITS1-PCR-RFLP and sequencing identified the causative agent of VL in Armenia as Leishmania infantum. The present work is an important step towards the inclusion of molecular techniques in the current diagnosis of VL in Armenia and the establishment of local molecular diagnostic facilities.</abstract>
    <parentTitle language="eng">Parasitology</parentTitle>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-12018</identifier>
    <identifier type="issn">1469-8161</identifier>
    <enrichment key="SourceTitle">Sukiasyan, A., Keshishyan, A., Manukyan, D., Melik-Andreasyan, G., Atshemyan, L., Apresyan, H., . . . Kuhls, K. (n.d.). Re-Emerging foci of visceral leishmaniasis in Armenia – first molecular diagnosis of clinical samples. Parasitology, 1-8. doi:10.1017/S0031182019000064</enrichment>
    <enrichment key="CopyrightInfo">This article has been published in a revised form in Parasitology [http://doi.org/10.1017/S0031182019000064]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. © Katrin Kuhls.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1017/S0031182019000064</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>Anna Sukiasyan</author>
    <author>Ara Keshishyan</author>
    <author>Dezdemonia Manukyan</author>
    <author>Gayane Melik-Andreasyan</author>
    <author>Liana Atshemyan</author>
    <author>Hripsime Apresyan</author>
    <author>Margarita V. Strelkova</author>
    <author>Marcus Frohme</author>
    <author>Sofia Cortes</author>
    <author>Katrin Kuhls</author>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="2">Green Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1201/Sukiasyan_et_al_2019_accepted_version.pdf</file>
  </doc>
  <doc>
    <id>1141</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>14</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro</title>
    <abstract language="eng">Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.&#13;
&#13;
Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function.&#13;
&#13;
The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.</abstract>
    <parentTitle language="eng">Nanoscale Research Letters</parentTitle>
    <identifier type="issn">1556-276X</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-11415</identifier>
    <enrichment key="SourceTitle">Grebinyk, A., Prylutska, S., Grebinyk, S. et al. Nanoscale Res Lett (2019) 14: 61. https://doi.org/10.1186/s11671-019-2894-1</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1186/s11671-019-2894-1</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Sergii Grebinyk</author>
    <author>Yuriy Prylutskyy</author>
    <author>Uwe Ritter</author>
    <author>Olga Matyshevska</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>C60 fullerene</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>doxorubicin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>noncovalent complex</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>leukemic cell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cytotoxicity</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>accumulation</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="Funding" number="">DFG-geförderter Publikationsfonds</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1141/2019_NRL_Grebinyk.pdf</file>
  </doc>
  <doc>
    <id>1130</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>6</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A Novel Microtiter Plate Format High Power Open Source LED Array</title>
    <abstract language="eng">Many photochemical or photobiological applications require the use of high power ultraviolet light sources, such as high-pressure mercury arc lamps. In addition, many photo-induced chemical, biochemical and biological applications require either a combinatorial setting or a parallel assay of multiple samples under the same environmental conditions to ensure reproducibility. To achieve this, alternative, controllable light sources, such as ultraviolet light emitting diodes (UV LEDs) with high power and spatial control are required. Preferably, LEDs are arranged in a suitable standardized 96-well microtiter plate format. We designed such an array and established the methods required for heat management and enabling stable, controllable illumination over time.</abstract>
    <parentTitle language="eng">Photonics</parentTitle>
    <identifier type="issn">2304-6732</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-11307</identifier>
    <enrichment key="SourceTitle">Kagel, H.; Jacobs, H.; Bier, F.F.; Glökler, J.; Frohme, M. A Novel Microtiter Plate Format High Power Open Source LED Array. Photonics 2019, 6, 17.</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.3390/photonics6010017</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Heike Kagel</author>
    <author>Hannes Jacobs</author>
    <author>Frank Fabian Bier</author>
    <author>Jörn Glökler</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>optics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>photonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>light</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>LED array</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>High-power LED</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>365 nm</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>microtiter plate</value>
    </subject>
    <collection role="ddc" number="621">Angewandte Physik</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1130/photonics-06-00017.pdf</file>
  </doc>
  <doc>
    <id>1096</id>
    <completedYear>2019</completedYear>
    <publishedYear/>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>14</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro</title>
    <abstract language="eng">Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.&#13;
&#13;
Here, we studied the physicochemical properties and anticancer activity of C60 fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 fullerene considerable nanocarrier function.&#13;
&#13;
The results of this study indicated that C60 fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.</abstract>
    <parentTitle language="eng">Nanoscale Research Letters</parentTitle>
    <identifier type="issn">1556-276X</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-10966</identifier>
    <note>Das Dokument wurde zurückgezogen. Die korrigierte Version ist unter dem folgenden Link zu finden: https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-11415</note>
    <enrichment key="SourceTitle">Grebinyk, A., Prylutska, S., Grebinyk, S. et al. Nanoscale Res Lett (2019) 14: 61. https://doi.org/10.1186/s11671-019-2894-1</enrichment>
    <enrichment key="RelatedIdentifier">https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-11415</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Anna Grebinyk</author>
    <author>Svitlana Prylutska</author>
    <author>Sergii Grebinyk</author>
    <author>Yuriy Prylutskyy</author>
    <author>Uwe Ritter</author>
    <author>Olga Matyshevska</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>C60 fullerene</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>doxorubicin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>noncovalent complex</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>leukemic cell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cytotoxicity</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>accumulation</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="green_open_access" number="1">Gold Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
  </doc>
  <doc>
    <id>1062</id>
    <completedYear>2019</completedYear>
    <publishedYear>2019</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A New Triple System DNA-Nanosilver-Berberine for Cancer Therapy</title>
    <abstract language="eng">The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA.In the current work we have investigated the effects of Berberine on the human T-cell leukemia cell line in vitro.Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time-and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction.Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers.The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanopoarticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment.</abstract>
    <parentTitle language="eng">Applied Nanoscience</parentTitle>
    <identifier type="issn">2190-5517</identifier>
    <identifier type="urn">urn:nbn:de:kobv:526-opus4-10628</identifier>
    <enrichment key="SourceTitle">Grebinyk, A., Yashchuk, V., Bashmakova, N., Gryn, D., Hagemann, T., Naumenko, A., et al. (2018). A new triple system DNA-Nanosilver-Berberine for cancer therapy. In Applied Nanoscience. http://dx.doi.org/10.1007/s13204-018-0688-x.</enrichment>
    <enrichment key="RelatedIdentifier">https://opus4.kobv.de/opus4-th-wildau/frontdoor/index/index/docId/1610</enrichment>
    <enrichment key="DOI_VoR">https://doi.org/10.1007/s13204-018-0688-x</enrichment>
    <licence>Das Dokument ist urheberrechtlich geschützt.</licence>
    <author>Anna Grebinyk</author>
    <author>Valeriy Yashchuk</author>
    <author>Nataliya Bashmakova</author>
    <author>Dmytro Gryn</author>
    <author>Tobias Hagemann</author>
    <author>Antonina Naumenko</author>
    <author>Nataliya Kutsevol</author>
    <author>Thomas Dandekar</author>
    <author>Marcus Frohme</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>berberine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>apoptosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>nanosilver</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DNA delivery system</value>
    </subject>
    <collection role="ddc" number="570">Biowissenschaften; Biologie</collection>
    <collection role="institutes" number="">Fachbereich Ingenieur- und Naturwissenschaften</collection>
    <collection role="open_access" number="">open_access</collection>
    <collection role="green_open_access" number="2">Green Open Access</collection>
    <thesisPublisher>Technische Hochschule Wildau</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-th-wildau/files/1062/Grebinyk_preprinted.pdf</file>
  </doc>
</export-example>
