@article{WegerichTuranoAllegrozzietal.2009, author = {Wegerich, F. and Turano, Paola and Allegrozzi, Marco and M{\"o}hwald, H. and Lisdat, Fred}, title = {Superoxide Biosensing with Engineered Cytochrome c}, series = {Procedia Chemistry}, volume = {1}, journal = {Procedia Chemistry}, number = {1}, issn = {1876-6196}, doi = {10.1016/j.proche.2009.07.321}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6523}, pages = {1287 -- 1290}, year = {2009}, abstract = {Several mutation positions have been chosen for introducing positively charged lysines in human cytochrome c (cyt c) with the aim of increasing the reaction rate with superoxide radicals (SO) and thus, the sensitivity of an electrochemical cyt c based SO biosensor. The impact of the mutations on structural and redox properties as well as on the reaction rate with SO are verified. Four mutants show a higher reaction rate with the radical compared to the wild type. These mutants are used for the construction of SO sensors based on thiol-modified gold electrodes and covalently fixed proteins. The E66K mutant electrode has a clearly higher sensitivity in comparison to the wildtype based sensor.}, language = {en} }