@article{LemieszewskiHannebauerRemiszewski2024, author = {Lemieszewski, Łukasz and Hannebauer, Dina and Remiszewski, Grzegorz}, title = {Vulnerability of Wi-Fi wireless network to signal interference}, series = {Journal of Engineering 360 (JoE 360)}, volume = {1}, journal = {Journal of Engineering 360 (JoE 360)}, number = {1/24}, publisher = {The Jacob of Paradies University}, issn = {3071-7507}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-19742}, pages = {58 -- 65}, year = {2024}, abstract = {Wi-Fi networks are often susceptible to signal interference that can disrupt services, intercept sensitive data, and allow unauthorized access. Exploiting this vulnerability, attackers can launch Intentional Electromagnetic Interference (IEMI) attacks or protocol-based attacks. IEMI attacks include, but are not limited to, jamming, which involves the introduction of interference signals in the frequency band used by a given Wi-Fi network, thus disrupting its proper operation. The article presents research on different types of IEMI attacks, aimed at illustrating this type of threat to video transmission from Wi-Fi cameras and discusses methods of protection against this type of attack. The paper examines the methods of jamming the wireless network band, which are used on the distribution methods and the Sweeping Jammer methods that cyclically disrupt all available devices. Based on experiments, it has been confirmed that SOHO devices are highly susceptible to this type of simple attacks and implementing a method to minimize the effects of jamming yields poor results. The simplicity of this type of attack and the high availability of software and hardware tools on the market make the threat highly popular and still dangerous.}, language = {en} } @inproceedings{LindnerLiebchen2019, author = {Lindner, Niels and Liebchen, Christian}, title = {New Perspectives on PESP: T-Partitions and Separators}, series = {19th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2019)}, booktitle = {19th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2019)}, publisher = {Schloss Dagstuhl, Leibniz-Zentrum f{\"u}r Informatik}, address = {Dagstuhl}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-20074}, pages = {2:1 -- 2:18}, year = {2019}, abstract = {In the planning process of public transportation companies, designing the timetable is among the core planning steps. In particular in the case of periodic (or cyclic) services, the Periodic Event Scheduling Problem (PESP) is well-established to compute high-quality periodic timetables. We are considering algorithms for computing good solutions and dual bounds for the very basic PESP with no additional extra features as add-ons. The first of these algorithms generalizes several primal heuristics that have been proposed, such as single-node cuts and the modulo network simplex algorithm. We consider partitions of the graph, and identify so-called delay cuts as a structure that allows to generalize several previous heuristics. In particular, when no more improving delay cut can be found, we already know that the other heuristics could not improve either. This heuristic already had been proven to be useful in computational experiments [Ralf Bornd{\"o}rfer et al., 2019], and we locate it in the more general concept of what we denote T-partitions. With the second of these algorithms we propose to turn a strategy, that has been discussed in the past, upside-down: Instead of gluing together the network line-by-line in a bottom-up way, we develop a divide-and-conquer-like top-down approach to separate the initial problem into two easier subproblems such that the information loss along their cutset edges is as small as possible. We are aware that there may be PESP instances that do not fit well the separator setting. Yet, on the RxLy-instances of PESPlib in our experimental computations, we come up with good primal solutions and dual bounds. In particular, on the largest instance (R4L4), this new separator approach, which applies a state-of-the-art solver as subroutine, is able to come up with better dual bounds than purely applying this state-of-the-art solver in the very same time.}, language = {en} } @inproceedings{SteglichVillringerDuemeckeetal.2015, author = {Steglich, Patrick and Villringer, Claus and D{\"u}mecke, Silvio and Padilla-Michel, Yazmin and Casalboni, Mauro and Schrader, Sigurd}, title = {Silicon-On-Insulator Slot-Waveguide Design Trade-Offs}, series = {Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology : vol. 2}, booktitle = {Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology : vol. 2}, publisher = {SciTePress}, address = {Set{\´u}bal}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-20104}, pages = {47 -- 52}, year = {2015}, abstract = {Silicon-on-insulator slot-waveguide structures are designed and analysed numerically. We present our theoretical investigation of field confinement factors and effective nonlinear areas for different waveguide structures in order to find optimized geometrical dimensions. It is shown that a slot-waveguide with a height of 220 nm, a slot width of 180 nm and a silicon rail width of 180 nm provides a five times higher field confinement in the cladding region compared to conventional strip-waveguides which explains the high sensitivity of slotwaveguide based label-free bio-sensors.}, language = {en} } @inproceedings{MalisaReiffStephan2025, author = {Malisa, Viktorio and Reiff-Stephan, J{\"o}rg}, title = {Auswirkung der neuen gesetzlichen und normativen Regelungen auf Industrieroboter-Systeme}, series = {Tagungsband AALE 2025 : menschenzentrierte Automation im digitalen Zeitalter}, booktitle = {Tagungsband AALE 2025 : menschenzentrierte Automation im digitalen Zeitalter}, editor = {Reiff-Stephan, J{\"o}rg and J{\"a}kel, Jens and Reichelt, Dirk}, publisher = {Hochschule f{\"u}r Technik, Wirtschaft und Kultur}, address = {Leipzig}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-20130}, pages = {49 -- 59}, year = {2025}, abstract = {EU-Verordnungen und ISO-Normen ver{\"a}ndern die Nutzung von Industrieroboter und beeinflussen deren Integration in effiziente Produktionssysteme. Die rasante Entwicklung der Digitalisierung und die Notwendigkeit einer umfassenden Vernetzung haben Auswirkungen auf die Sicherheit von Maschinen, die Gestaltung von Arbeitspl{\"a}tzen und die Anforderungen an die Ausbildung der Besch{\"a}ftigten. Mit einer Reihe von Gesetzen und Richtlinien schafft die EU einen Ordnungsrahmen f{\"u}r die aktuellen Technologien und versucht, die Rahmenbedingungen f{\"u}r weitere Entwicklungen festzulegen. Gleichzeitig ist die Industrie gefordert, zus{\"a}tzliche Kompetenzen in bestimmten Bereichen der Digitalisierung aufzubauen. Einige Herausforderungen sind jedoch noch zu bew{\"a}ltigen, z. B. wie der Sicherheitsnachweis f{\"u}r in Maschinen integrierte K{\"u}nstliche Intelligenz f{\"u}r autonome mobile Roboter erbracht werden kann, wie die Zertifizierung von Software als Sicherheitselement und dessen Integration in die Automatisierungssysteme und wie die Behandlung von Industrierobotern als vollst{\"a}ndige und nicht mehr als unvollst{\"a}ndige Maschinen erfolgen wird.}, language = {de} } @inproceedings{PadillaMichelLucciCasalbonietal.2015, author = {Padilla-Michel, Yazmin and Lucci, Massimiliano and Casalboni, Mauro and Steglich, Patrick and Schrader, Sigurd}, title = {Mechanical Characterisation of the Four Most Used Coating Materials for Optical Fibres}, series = {Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology : vol. 1}, booktitle = {Proceedings of the 3rd International Conference on Photonics, Optics and Laser Technology : vol. 1}, editor = {Ribeiro, Paulo A.}, publisher = {SciTePress}, address = {Set{\´u}bal}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-20063}, pages = {96 -- 102}, year = {2015}, abstract = {Optical multimode fibres have a wide variety of applications ranging from industrial to medical use. Therefore, even if they are just used as waveguides or sensors, it is important to characterise the whole fingerprint, including the optical and mechanical properties of such fibres. Since the stiffness/elasticity of a material could influence the optical output of a fibre due to micro-bendings, in this paper we report the calculated Young's Modulus of acrylate, fluorinated acrylate, polyimide and silicone, which are the four most used coating materials for such optical components. The results demonstrate that Young's Modulus does have an impact on the attenuation of propagating light along the optical fibre. However, the refractive index of the coating materials still has a significant impact on the performance of optical fibres.}, language = {en} } @inproceedings{KnotheGeringGloddeetal.2022, author = {Knothe, Thomas and Gering, Patrick and Glodde, Ilona and Ahle, Ulrich and B{\"o}ttger, Jonas and D{\"o}beritz, Niklas}, title = {Model based Configuration of Platforms for Managing Cross- Organizational (Business) Processes}, series = {Interoperability for Enterprise Systems and Applications I-ESA workshops 2022}, booktitle = {Interoperability for Enterprise Systems and Applications I-ESA workshops 2022}, editor = {Zelm, Martin and Boza, Andr{\´e}s and Le{\´o}n, Ramona-Diana and Rodriguez-Rodriguez, Raul}, publisher = {RWTH Aachen}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18993}, pages = {1 -- 6}, year = {2022}, abstract = {In this contribution interoperability is considered from the perspective of platforms, which have to manage cross-organisational business processes. A model-based approach for configuring a cloud platform for managing complex processes and their dependencies across different organisations is provided. The approach is applied on using FIWARE, which provides a framework of open source software platform components. The core concept is to extend the open source core data model of FIWARE by using the artefacts of an Enterprise model, describing the dependencies of processes, roles, object data and application interfaces. Based on a given use case the principal configuration was applied and validated.}, language = {en} } @article{TillichWolterSchulzeetal.2014, author = {Tillich, Ulrich M. and Wolter, Nick and Schulze, Katja and Kramer, Dan and Br{\"o}del, Oliver and Frohme, Marcus}, title = {High-throughput cultivation and screening platform for unicellular phototrophs}, series = {BMC Microbiology}, volume = {14}, journal = {BMC Microbiology}, number = {239}, issn = {1471-2180}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6007}, pages = {13}, year = {2014}, abstract = {In this work we describe and test high-throughput methods with the model organism Synechocystis sp. PCC6803. The required technical automation for these processes was achieved with a Tecan Freedom Evo 200 pipetting robot. The cultivation was performed in 2.2 ml deepwell microtiter plates within a cultivation chamber outfitted with programmable shaking conditions, variable illumination, variable temperature, and an adjustable CO2 atmosphere. Each microtiter-well within the chamber functions as a separate cultivation vessel with reproducible conditions. The automated measurement of various parameters such as growth, full absorption spectrum, chlorophyll concentration, MALDI-TOF-MS, as well as a novel vitality measurement protocol, have already been established and can be monitored during cultivation. Measurement of growth parameters can be used as inputs for the system to allow for periodic automatic dilutions and therefore a semi-continuous cultivation of hundreds of cultures in parallel. The system also allows the automatic generation of mid and long term backups of cultures to repeat experiments or to retrieve strains of interest.}, language = {en} } @article{HerzogJaenchen2016, author = {Herzog, Thomas H. and J{\"a}nchen, Jochen}, title = {Adsorption Properties of Modified Zeolites for Operating Range Enhancement of Adsorption Heat Pumps through the Use of Organic Adsorptive Agents}, series = {Energy Procedia}, volume = {91}, journal = {Energy Procedia}, issn = {1876-6102}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6614}, pages = {155 -- 160}, year = {2016}, abstract = {Faujasite zeolites with the highest possible Al-content and different cations as well as a dealuminated zeolite Y have been chosen to study the impact on the adsorption behavior in view of differed adsorptive agents for heat pumps or thermochemical storages. Our results show advantages and disadvantages for water, methanol or ethanol adsorption due to the structure of the anion skeleton, kind of cations and size of the adsorbat molecule.}, language = {en} } @article{Michalk2013, author = {Michalk, Philip}, title = {Optimizing sales areas of combined transport chains}, series = {LogForum}, volume = {9}, journal = {LogForum}, number = {4}, issn = {1734-459X}, doi = {10.15771/1734-459X_2013_1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6252}, pages = {247 -- 253}, year = {2013}, abstract = {Background: Combined transport chains (such as intermodal transport), have certain advantages. The main advantage from customer points of view is the possibility to bundle freight and thereby decrease transport costs. On the other hand, a combined transport chain can cause longer transport times, due to the necessary transshipment processes. Methods: The area around a terminal, in which a combined service has favourable properties to a customer in comparison to a direct transport, can be understood as a sales-area, in which a combined transport product is marketable. The aim of this paper was to find a method to determine the best shape and size of this area. Results and conclusions: The paper at hand lined out a method in order to calculate such a sales area and determine which geographical points around a terminal have an advantage in comparison to a direct transport service.}, language = {en} } @article{Enders2016, author = {Enders, Peter}, title = {Historical Prospective: Boltzmann's versus Planck's State Counting—Why Boltzmann Did Not Arrive at Planck's Distribution Law}, series = {Journal of Thermodynamics}, volume = {2016}, journal = {Journal of Thermodynamics}, issn = {1687-9252}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6281}, pages = {13}, year = {2016}, abstract = {Why does Planck (1900), referring to Boltzmann's 1877 probabilistic treatment, obtain his quantum distribution function while Boltzmann did not? To answer this question, both treatments are compared on the basis of Boltzmann's 1868 three-level scheme (configuration—occupation—occupancy). Some calculations by Planck (1900, 1901, and 1913) and Einstein (1907) are also sketched. For obtaining a quantum distribution, it is crucial to stick with a discrete energy spectrum and to make the limit transitions to infinity at the right place. For correct state counting, the concept of interchangeability of particles is superior to that of indistinguishability.}, language = {en} } @article{BelinskyRinkCaietal.2015, author = {Belinsky, Martin G. and Rink, Lori and Cai, Kathy Q. and Capuzzi, Stephen J. and Hoang, Yen and Chien, Jeremy and Godwin, Andrew K. and von Mehren, Margaret}, title = {Somatic loss of function mutations in neurofibromin 1 and MYC associated factor X genes identified by exome-wide sequencing in a wild-type GIST case}, series = {BMC Cancer}, volume = {15}, journal = {BMC Cancer}, number = {887}, issn = {1471-2407}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6292}, year = {2015}, abstract = {Approximately 10-15 \% of gastrointestinal stromal tumors (GISTs) lack gain of function mutations in the KIT and platelet-derived growth factor receptor alpha (PDGFRA) genes. An alternate mechanism of oncogenesis through loss of function of the succinate-dehydrogenase (SDH) enzyme complex has been identified for a subset of these "wild type" GISTs.}, language = {en} } @article{MunchelHoangYueetal.2015, author = {Munchel, Sarah and Hoang, Yen and Yue, Zhao and Cottrell, Joseph and Klotzle, Brandy and Godwin, Andrew K. and Koestler, Devin and Beyerlein, Peter and Fan, Jian-Bing and Bibikova, Marina and Chien, Jeremy}, title = {Targeted or whole genome sequencing of formalin fixed tissue samples: potential applications in cancer genomics}, series = {Oncotarget}, volume = {6}, journal = {Oncotarget}, number = {28}, issn = {1949-2553}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6307}, pages = {25943 -- 25961}, year = {2015}, abstract = {Current genomic studies are limited by the poor availability of fresh-frozen tissue samples. Although formalin-fixed diagnostic samples are in abundance, they are seldom used in current genomic studies because of the concern of formalin-fixation artifacts. Better characterization of these artifacts will allow the use of archived clinical specimens in translational and clinical research studies. To provide a systematic analysis of formalin-fixation artifacts on Illumina sequencing, we generated 26 DNA sequencing data sets from 13 pairs of matched formalin-fixed paraffin-embedded (FFPE) and fresh-frozen (FF) tissue samples. The results indicate high rate of concordant calls between matched FF/FFPE pairs at reference and variant positions in three commonly used sequencing approaches (whole genome, whole exome, and targeted exon sequencing). Global mismatch rates and C·G > T·A substitutions were comparable between matched FF/FFPE samples, and discordant rates were low (<0.26\%) in all samples. Finally, low-pass whole genome sequencing produces similar pattern of copy number alterations between FF/FFPE pairs. The results from our studies suggest the potential use of diagnostic FFPE samples for cancer genomic studies to characterize and catalog variations in cancer genomes.}, language = {en} } @article{GrawMeierMinnetal.2015, author = {Graw, Stefan and Meier, Richard and Minn, Kay and Bloomer, Clark and Godwin, Andrew K. and Fridley, Brooke and Vlad, Anda and Beyerlein, Peter and Chien, Jeremy}, title = {Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples}, series = {Scientific Reports}, volume = {5}, journal = {Scientific Reports}, issn = {2045-2322}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6319}, pages = {11}, year = {2015}, abstract = {Current genomic studies are limited by the availability of fresh tissue samples. Here, we show that Illumina RNA sequencing of formalin-fixed diagnostic tumor samples produces gene expression that is strongly correlated with matched frozen tumor samples (r > 0.89). In addition, sequence variations identified from FFPE RNA show 99.67\% concordance with that from exome sequencing of matched frozen tumor samples. Because FFPE is a routine diagnostic sample preparation, the feasibility results reported here will facilitate the setup of large-scale research and clinical studies in medical genomics that are currently limited by the availability of fresh frozen samples.}, language = {en} } @article{KipkorirMuhohoMuliroetal.2015, author = {Kipkorir, R. and Muhoho, S. and Muliro, P. and Mugendi, Beatrice and Frohme, Marcus and Br{\"o}del, Oliver}, title = {Effects of Coffee Processing Technologies on Aroma Profiles and Sensory Quality of Ruiru 11 and SL 28 Kenyan Coffee Varieties}, series = {Asian Journal of Agriculture and Food Sciences}, volume = {3}, journal = {Asian Journal of Agriculture and Food Sciences}, number = {2}, issn = {2321-1571}, doi = {10.15771/2321-1571_2015_1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6482}, pages = {178 -- 188}, year = {2015}, abstract = {The study aimed at comparing the effects of three coffee processing methods on aroma profiles and sensory quality of Ruiru 11 and SL 28 coffee varieties. The processing methods varied on stages of processing and method of mucilage removal. The green coffee beans obtained from the three processing methods were graded and roasted, ground and analyzed for the aroma profiles and sensory quality. Headspace Solid phase Microextraction fibre (SPME) technique were used for the extraction of aroma compounds from coffee samples and characterization of the compounds with use of gas chromatography mass spectrometry (GC-MS). Sensory quality were analyzed by an expert panelist. Various volatile aroma compounds were identified in roasted coffee and classified into their chemical classes involving furans, ketones, pyrazines ketones pyridines, pyrroles and acids. The intensity of aroma compounds were compared in terms of their peak areas and variations were noted between the processing methods with the ecopulper showing higher levels of pyrazines such as 2-methylpyrazine, 2-ethyl-6-methylpyrazine, and wet pulper showing higher levels of furans such as furfuryl formate and furfuryl alcohol, acetate. The hand pulper was high in the level of acids and esters such as acetic acid and propanoic acid, ethyl ester. Similar aroma compounds were identified in headspace of Ruiru 11 and SL 28 and there were variations in the intensities of aroma groups such as pyrroles and pyridines. It was concluded that the eco-pulper and hand pulper methods gives better aroma quality than the wet pulper while SL 28 variety gives higher sensory quality than Ruiru 11.}, language = {en} } @article{HaiderAschauerSchmidt2014, author = {Haider, Christian and Aschauer, Gerald J. and Schmidt, Conrad}, title = {IT Based Attempt to Evaluate and Promote Intermodal Transport Solutions in Central and Southeast Europe}, series = {Transportation Research Procedia}, volume = {3}, journal = {Transportation Research Procedia}, issn = {2352-1465}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6535}, pages = {641 -- 650}, year = {2014}, abstract = {The transport sector is one of the main emitters of greenhouse gas emissions. The awareness about this is increasing because of heightened environmental sensitivity among customers, politicians and the media. Studies dealing with sustainability in logistics have been inducted to show that companies around the world are keen to promote "green solutions" through the management of logistics. From now, it is difficult to gauge how far these efforts reflect true desire to help the environment as opposed to enhance public relations. The paper addresses an IT based attempt to evaluate and promote intermodal transport procedures in the corridor of Central and Southeast Europe. Through the assessment of the relative importance of intermodal transport nodes the described tool is a useful instrument to demonstrate the most important advantages for environmental friendly transport solutions. In combination with accessibility as well as network scenario attempts stakeholders have full information available when preparing several transport plans due to transparent strengths of rail and inland waterway transports.}, language = {en} } @article{KaltdorfSchulzeHelmprobstetal.2017, author = {Kaltdorf, Kristin Verena and Schulze, Katja and Helmprobst, Frederik and Kollmannsberger, Philip and Dandekar, Thomas and Stigloher, Christian}, title = {FIJI Macro 3D ART VeSElecT: 3D Automated Reconstruction Tool for Vesicle Structures of Electron Tomograms}, series = {PLoS Computational Biology}, volume = {13}, journal = {PLoS Computational Biology}, number = {1}, issn = {1553-7358}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-9218}, year = {2017}, abstract = {Automatic image reconstruction is critical to cope with steadily increasing data from advanced microscopy. We describe here the Fiji macro 3D ART VeSElecT which we developed to study synaptic vesicles in electron tomograms. We apply this tool to quantify vesicle properties (i) in embryonic Danio rerio 4 and 8 days past fertilization (dpf) and (ii) to compare Caenorhabditis elegans N2 neuromuscular junctions (NMJ) wild-type and its septin mutant (unc-59(e261)). We demonstrate development-specific and mutant-specific changes in synaptic vesicle pools in both models. We confirm the functionality of our macro by applying our 3D ART VeSElecT on zebrafish NMJ showing smaller vesicles in 8 dpf embryos then 4 dpf, which was validated by manual reconstruction of the vesicle pool. Furthermore, we analyze the impact of C. elegans septin mutant unc-59(e261) on vesicle pool formation and vesicle size. Automated vesicle registration and characterization was implemented in Fiji as two macros (registration and measurement). This flexible arrangement allows in particular reducing false positives by an optional manual revision step. Preprocessing and contrast enhancement work on image-stacks of 1nm/pixel in x and y direction. Semi-automated cell selection was integrated. 3D ART VeSElecT removes interfering components, detects vesicles by 3D segmentation and calculates vesicle volume and diameter (spherical approximation, inner/outer diameter). Results are collected in color using the RoiManager plugin including the possibility of manual removal of non-matching confounder vesicles. Detailed evaluation considered performance (detected vesicles) and specificity (true vesicles) as well as precision and recall. We furthermore show gain in segmentation and morphological filtering compared to learning based methods and a large time gain compared to manual segmentation. 3D ART VeSElecT shows small error rates and its speed gain can be up to 68 times faster in comparison to manual annotation. Both automatic and semi-automatic modes are explained including a tutorial.}, language = {en} } @article{PraiaHerkenhoffBroedeletal.2022, author = {Praia, Ana Beatriz and Herkenhoff, Marcos Edgar and Br{\"o}del, Oliver and Frohme, Marcus and Saad, Susana Marta Isay}, title = {Sour Beer with Lacticaseibacillus paracasei subsp. paracasei F19: Feasibility and Influence of Supplementation with Spondias mombin L. Juice and/or By-Product}, series = {Foods}, volume = {11}, journal = {Foods}, number = {24}, publisher = {MDPI}, issn = {2304-8158}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16852}, year = {2022}, abstract = {This study aimed to evaluate the probiotic strain Lacticaseibacillus (L.) paracasei subsp. paracasei F19 (F19) with the yeast Saccharomyces cerevisiae US-05 (US-05), using Spondias mombin L. ('tapereb{\´a}' or 'caj{\´a}') juice and by-product, in four sour-type beer formulations: control, with bagasse, juice, and juice and bagasse. The viability of F19 was evaluated by pour-plating and PMA-qPCR. Fermentability, in addition to physicochemical and sensory parameters, and aroma and flavor, were evaluated during brewery by using Headspace Solid-Phase Microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). F19 was successful in fermenting bagasse in a MRS medium (9.28 log CFU/mL in 24 h) but had a low viability in hopped wort, growing better in formulations without bagasse or juice. No difference between formulations was observed regarding sensory acceptability, and the HS-SPME/GC-MS revealed different flavors and aroma compounds. In conclusion, the production of a potential probiotic sour beer with F19 and US-05 is feasible regarding probiotic viability. However, S. mombin, as juice or bagasse, threatened probiotic survival. Different flavors and aroma compounds were detected, whereas no difference between formulations was found regarding sensory acceptability. The moderate alcohol content achieved is important for bacterial survival and for the development of a probiotic beer with health claims.}, language = {en} } @article{RadivoievychKolpGrebinyketal.2023, author = {Radivoievych, Aleksandar and Kolp, Benjamin and Grebinyk, Sergii and Prylutska, Svitlana and Ritter, Uwe and Zolk, Oliver and Gl{\"o}kler, J{\"o}rn and Frohme, Marcus and Grebinyk, Anna}, title = {Silent Death by Sound: C60 Fullerene Sonodynamic Treatment of Cancer Cells}, series = {International Journal of Molecular Sciences}, volume = {24}, journal = {International Journal of Molecular Sciences}, number = {2}, publisher = {MDPI}, issn = {1422-0067}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16877}, year = {2023}, abstract = {The acoustic pressure waves of ultrasound (US) not only penetrate biological tissues deeper than light, but they also generate light emission, termed sonoluminescence. This promoted the idea of its use as an alternative energy source for photosensitizer excitation. Pristine C60 fullerene (C60), an excellent photosensitizer, was explored in the frame of cancer sonodynamic therapy (SDT). For that purpose, we analyzed C60 effects on human cervix carcinoma HeLa cells in combination with a low-intensity US treatment. The time-dependent accumulation of C60 in HeLa cells reached its maximum at 24 h (800 ± 66 ng/106 cells). Half of extranuclear C60 is localized within mitochondria. The efficiency of the C60 nanostructure's sonoexcitation with 1 MHz US was tested with cell-based assays. A significant proapoptotic sonotoxic effect of C60 was found for HeLa cells. C60′s ability to induce apoptosis of carcinoma cells after sonoexcitation with US provides a promising novel approach for cancer treatment.}, language = {en} } @unpublished{Liebchen2021, author = {Liebchen, Christian}, title = {Maximizing Bidirectional Green Waves for Major Road Axes}, doi = {10.15771/2827}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15449}, pages = {1 -- 20}, year = {2021}, abstract = {Both, from an environmental perspective and with respect to road traffic flow quality, planning so-called green waves along major road axes is a well-established target for traffic engineers. For one-way road axes (e.g. the Avenues in Manhattan), this is a trivial downstream task. For bidirectional arterials, the well-known necessary condition for establishing a green wave in both direction is that the driving times between two subsequent crossings must be an integer multiple of half of the cycle time of the signal programs at the nodes. In this paper, we propose an integer linear optimization model to establish fixed-time green waves in both directions that are as long and as wide as possible, even in the situation where the driving time condition is not fulfilled. In particular, we are considering an arterial along whose nodes separate left-turn signal groups are realized. In our computational results, we provide examples which illustrate that allowing for selecting among different strategies for the left-turn phases is beneficial. Moreover, we show that there is always a solution with green waves in both directions that are as long and as wide as possible, where absolute priority is put on just one direction. Only when considering prioritized parts of a green band (e.g. some first few seconds), then an ideal green wave into one direction can provide suboptimal quality compared to optimizing both directions together.}, language = {en} } @article{VillringerSteglichPulweretal.2021, author = {Villringer, Claus and Steglich, Patrick and Pulwer, Silvio and Schrader, Sigurd and Laufer, Jan}, title = {Electro-optical properties of doped polymers with high transparency in the visible wavelength range}, series = {Optical Materials Express}, volume = {11}, journal = {Optical Materials Express}, number = {11}, publisher = {Optica Publishing Group}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15480}, pages = {3801 -- 3811}, year = {2021}, abstract = {The electro-optical (EO) properties of poly(methyl methacrylate) and the photopolymer poly(vinyl cinnamate) doped with varying concentrations of the EO chromophore 2-Methyl-4-nitroaniline were measured. The EO polymers were embedded in Fabry-P{\´e}rot etalons for the simultaneous determination of the Pockels and Kerr coefficients from measurements of the fringe shift induced by an external electric field. It was found that the host polymer has a significant impact on the EO performance and that the undoped host polymers exhibit a significant Pockels effect. Moreover, the Kerr effect provides a substantial contribution of 27\% to the total change of the refractive index at relatively high electric field strengths of E = 91.2 MV m-1.}, language = {en} } @phdthesis{Arndt2010, author = {Arndt, Wulf-Holger}, title = {Optimierungspotenziale im Wirtschaftsverkehr durch bestellerseitige Kooperation}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15497}, school = {Technische Hochschule Wildau}, pages = {281}, year = {2010}, abstract = {Es wird ein neuer Ansatz zur Verkehrs- und Kostenreduzierung im Wirtschaftsverkehr (genauer Lieferverkehr) untersucht. Die virtuelle Zusammenfassung von Bestellungen z.B. {\"u}ber eine Internet-/Intranetplattform - eine Empf{\"a}nger/Bestellerkooperation kann m{\"o}glicherweise zur B{\"u}ndelung der Lieferverkehre f{\"u}hren. Aufgrund von neuen Produktionsmethoden und Managementstrategien zur St{\"a}rkung der Eigenverantwortlichkeit von Unternehmensbereichen und Abteilungen laufen Bestellprozesse oft separat und unkoordiniert ab. Eine Folge ist die Zersplitterung der Bestellungen und die geringe Auslastung von Lieferverkehren. Neben dieser Dezentralisierung f{\"u}hrt „Outsourcen" dazu, dass Bestellungen immer kleinteiliger und weniger zusammengefasst werden. Um trotzdem Kosten zu sparen versuchen Lieferanten B{\"u}ndelungen durchzuf{\"u}hren. Dies geschieht bei Spedition am ehesten unternehmensintern aber auch in Netzwerken oder bei der City-Logistik auch {\"u}berbetrieblich als Versenderkooperation. Dies ist aber nur die eine Seite des Beschaffungsprozesses. Außerdem ist der Versenderkooperation durch Konkurrenzsituationen Grenzen gesetzt. Auf der anderen Seite ist eine B{\"u}ndelung (der Bestellungen) ebenfalls denkbar, wenn sich daraus Vorteile f{\"u}r die Besteller ergeben, z.B. Preisreduktionen aufgrund gr{\"o}ßerer Bestellmengen. Das w{\"a}re eine Besteller- bzw. Empf{\"a}ngerkooperation. Die Besteller/Empf{\"a}nger schließen sich zusammen und nehmen durch die Bestellungsb{\"u}ndelung den Versendern und Transporteuren quasi die Abstimmungs-Probleme einer Kooperation untereinander teilweise ab. Werden Lieferprozesse mit in die Kooperation integriert, handelt es sich um eine Beschaffungs-kooperation. Fr{\"u}her war eine solche B{\"u}ndelung auf Bestellerseite durch große bestellende Einheiten (Werksgr{\"o}ße, Zentralisierung, hohe Produktionstiefe,...) per se gegeben. Diese Produktions-methoden sind nicht einfach wieder herstellbar. Eine virtuelle B{\"u}ndelung z.B. mittels E Procurement (u.a. Internet-Bestellplattform) w{\"a}re heute zeitgem{\"a}ß. Diese realisiert quasi eine virtuelle Zentralbestellung innerbetrieblich f{\"u}r dezentral organisierte Strukturen oder {\"u}berbetrieblich zwischen unabh{\"a}ngigen Unternehmen. Die haupts{\"a}chlichen Vorteile einer solchen Kooperation sind neben Prozessvereinfachung, Transparenzerh{\"o}hung sowie Verbesserung der Steuerung und Kontrolle bei der Beschaffung, Kosteneinsparung durch Aufwandsminimierung und Nutzung von Preisrabatten durch B{\"u}ndelung der Bestellmengen von mehreren Bestellern. Im Wirtschaftsverkehr sind Effizienzsteigerung und Verkehrsreduzierung durch Kooperationen noch wenig untersucht. Insbesondere Planungsinstrumente zur F{\"o}rderung solcher Kooperationen m{\"u}ssen entwickelt werden. Innerhalb dieser Arbeit fanden umfangreiche Erhebungen statt. In Felduntersuchungen an der Technische Universit{\"a}t Berlin, verschiedenen Gewerbegebieten, Handelseinrichtungen (Shop-in-Shop-Systemen) und B{\"u}roh{\"a}usern wurden Beschaffungsprozesse, Organisationsprobleme und Kooperationsneigungen analysiert. Auf Grundlage der Erkenntnisse aus diesen quantitativen Befragungen und zus{\"a}tzlichen qualitativen Experteninterviews zur Beschaffung sind verschiedene Varianten des Ansatzes entwickelt worden. In einer abschließenden deutschlandweiten Online-Unternehmensbefragung mit 1.700 auswertbaren Antworten, einer Conjoint-Analyse und weiteren Experteninterviews wurden verkehrliche Wirkungen untersucht, Kooperationsans{\"a}tze bewertet und weiter entwickelt.}, language = {de} } @article{RoosHoqueKlimešovaetal.2023, author = {Roos, Andreas Hult and Hoque, Ziaul and Klimešov{\´a}, Eva and Ben Ltaief, Ltaief and Medina, Cristian and Jurkovič, Matej and Albrecht, Martin and Finke, Ondřej and Hort, Ondřej and Nejdl, Jaroslav and Mudrich, Marcel and Andreasson, Jakob and Krikunova, Maria}, title = {Electron correlation dynamics in atomic Kr excited by XUV pulses and controlled by NIR laser pulses of variable intensity}, series = {New Journal of Physics}, volume = {25}, journal = {New Journal of Physics}, publisher = {Institute of Physics Publishing (IOP)}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16987}, year = {2023}, abstract = {We have investigated the possibility to track and control correlation dynamics of valence electrons in krypton (Kr) initiated by the absorption of one extreme ultraviolet (XUV) photon. In this investigation, pump-probe experiments have been performed where monochromatized single high-harmonics at photon energies 29.6, 32.8, and 35.9 eV have been used as pump to populate different intermediate excited states. A temporally delayed near-infrared (NIR) pulse probes the population of various decay channels via the detection of Kr²⁺ ion yields and its transient profiles. We observe that by varying the NIR pulse intensity within a range from 0.3 x 10¹³ to 2.6 x 10¹³ W cm⁻², the shape of the Kr²+ transient profile changes significantly. We show that by varying the intensity of the NIR pulse, it is possible—(i) to control the ratio between sequential and non-sequential double ionization of Kr; (ii) to selectively probe quantum beating oscillations between Kr+* satellite states that are coherently excited within the bandwidth of the XUV pulse; and (iii) to specifically probe the relaxation dynamics of doubly excited (Kr**) decay channels. Our studies show that the contribution of different ionization and decay channels (i)-(iii) can be altered by the NIR pulse intensity, thus demonstrating an efficient way to control the ionization dynamics in rare gas atoms.}, language = {en} } @article{AnandSchubertCorleyWiciaketal.2023, author = {Anand, Ketan and Schubert, Markus Andreas and Corley-Wiciak, Agnieszka Anna and Spirito, Davide and Corley-Wiciak, Cedric and Klesse, Wolfgang M. and Mai, Andreas and Tillack, Bernd and Yamamoto, Yuji}, title = {Lateral Selective SiGe Growth for Local Dislocation-Free SiGe-on-Insulator Virtual Substrate Fabrication}, series = {ECS Journal of Solid State Science and Technology}, volume = {12}, journal = {ECS Journal of Solid State Science and Technology}, publisher = {Electrochemical Society (ECS); IOP}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17013}, year = {2023}, abstract = {Dislocation free local SiGe-on-insulator (SGOI) virtual substrate is fabricated using lateral selective SiGe growth by reduced pressure chemical vapor deposition. The lateral selective SiGe growth is performed around a ∼1.25 μm square Si (001) pillar in a cavity formed by HCl vapor phase etching of Si at 850 °C from side of SiO2/Si mesa structure on buried oxide. Smooth root mean square roughness of SiGe surface of 0.14 nm, which is determined by interface roughness between the sacrificially etched Si and the SiO2 cap, is obtained. Uniform Ge content of ∼40\% in the laterally grown SiGe is observed. In the Si pillar, tensile strain of ∼0.65\% is found which could be due to thermal expansion difference between SiO2 and Si. In the SiGe, tensile strain of ∼1.4\% along 〈010〉 direction, which is higher compared to that along 〈110〉 direction, is observed. The tensile strain is induced from both [110] and [-110] directions. Threading dislocations in the SiGe are located only ∼400 nm from Si pillar and stacking faults are running towards 〈110〉 directions, resulting in the formation of a wide dislocation-free area in SiGe along 〈010〉 due to horizontal aspect ratio trapping.}, language = {en} } @article{MorlockSubramanianZounietal.2023, author = {Morlock, Sascha and Subramanian, Senthil Kumar and Zouni, Athina and Lisdat, Fred}, title = {Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes}, series = {Chemical Science}, volume = {14}, journal = {Chemical Science}, publisher = {Royal Society of Chemistry (RSC)}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17022}, pages = {1696 -- 1708}, year = {2023}, abstract = {One restriction for biohybrid photovoltaics is the limited conversion of green light by most natural photoactive components. The present study aims to fill the green gap of photosystem I (PSI) with covalently linked fluorophores, ATTO 590 and ATTO 532. Photobiocathodes are prepared by combining a 20 μm thick 3D indium tin oxide (ITO) structure with these constructs to enhance the photocurrent density compared to setups based on native PSI. To this end, two electron transfer mechanisms, with and without a mediator, are studied to evaluate differences in the behavior of the constructs. Wavelength-dependent measurements confirm the influence of the additional fluorophores on the photocurrent. The performance is significantly increased for all modifications compared to native PSI when cytochrome c is present as a redox-mediator. The photocurrent almost doubles from -32.5 to up to -60.9 μA cm-2. For mediator-less photobiocathodes, interestingly, drastic differences appear between the constructs made with various dyes. While the turnover frequency (TOF) is doubled to 10 e-/PSI/s for PSI-ATTO590 on the 3D ITO compared to the reference specimen, the photocurrents are slightly smaller since the PSI-ATTO590 coverage is low. In contrast, the PSI-ATTO532 construct performs exceptionally well. The TOF increases to 31 e-/PSI/s, and a photocurrent of -47.0 μA cm-2 is obtained. This current is a factor of 6 better than the reference made with native PSI in direct electron transfer mode and sets a new record for mediator-free photobioelectrodes combining 3D electrode structures and light-converting biocomponents.}, language = {en} } @article{KoenigKiffnerKuhlsetal.2023, author = {K{\"o}nig, Hannes J. and Kiffner, Christian and Kuhls, Katrin and Uthes, Sandra and Harms, Verena and Wieland, Ralf}, title = {Planning for wolf-livestock coexistence: landscape context predicts livestock depredation risk in agricultural landscapes}, series = {animal}, volume = {17}, journal = {animal}, number = {3}, publisher = {Elsevier}, issn = {1751-7311}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17032}, year = {2023}, abstract = {Extensive pastoral livestock systems in Central Europe provide multiple ecosystem services and support biodiversity in agricultural landscapes but their viability is challenged by livestock depredation (LD) associated with the recovery of wolf populations. Variation in the spatial distribution of LD depends on a suite of factors, most of which are unavailable at the appropriate scales. To assess if LD patterns can be predicted sufficiently with land use data alone at the scale of one federal state in Germany, we employed a machine-learning-supported resource selection approach. The model used LD monitoring data, and publicly available land use data to describe the landscape configuration at LD and control sites (resolution 4 km * 4 km). We used SHapley Additive exPlanations to assess the importance and effects of landscape configuration and cross-validation to evaluate the model performance. Our model predicted the spatial distribution of LD events with a mean accuracy of 74\%. The most influential land use features included grassland, farmland and forest. The risk of livestock depredation was high if these three landscape features co-occurred with a specific proportion. A high share of grassland, combined with a moderate proportion of forest and farmland, increased LD risk. We then used the model to predict the LD risk in five regions; the resulting risk maps showed high congruence with observed LD events. While of correlative nature and lacking specific information on wolf and livestock distribution and husbandry practices, our pragmatic modelling approach can guide spatial prioritisation of damage prevention or mitigation practices to improve livestock-wolf coexistence in agricultural landscapes.}, language = {en} } @article{FalkElGhayedvandeSandetal.2023, author = {Falk, Constantin and El Ghayed, Tarek and van de Sand, Ron and Reiff-Stephan, J{\"o}rg}, title = {A Data-Driven Approach Towards the Application of Reinforcement Learning Based HVAC Control}, series = {Journal of the Nigerian Society of Physical Sciences}, volume = {5}, journal = {Journal of the Nigerian Society of Physical Sciences}, number = {1}, publisher = {Department of Physics, Federal University Lafia Nasarawa State, Nigeria ; Nigerian Society of Physical Sciences}, issn = {2714-4704}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17066}, year = {2023}, abstract = {Refrigeration applications consume a significant share of total electricity demand, with a high indirect impact on global warming through greenhouse gas emissions. Modern technology can help reduce the high power consumption and optimize the cooling control. This paper presents a case study of machine-learning for controlling a commercial refrigeration system. In particular, an approach to reinforcement learning is implemented, trained and validated utilizing a model of a real chiller plant. The reinforcement-learning controller learns to operate the plant based on its interactions with the modeled environment. The validation demonstrates the functionality of the approach, saving around 7\% of the energy demand of the reference control. Limitations of the approach were identified in the discretization of the real environment and further model-based simplifications and should be addressed in future research.}, language = {en} } @article{AlekseevaNechyporenkoFrohmeetal.2023, author = {Alekseeva, Victoriia and Nechyporenko, Alina and Frohme, Marcus and Gargin, Vitaliy and Meniailov, Ievgen and Chumachenko, Dmytro}, title = {Intelligent Decision Support System for Differential Diagnosis of Chronic Odontogenic Rhinosinusitis Based on U-Net Segmentation}, series = {Electronics}, volume = {12}, journal = {Electronics}, number = {5}, publisher = {MDPI}, issn = {2079-9292}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17088}, year = {2023}, abstract = {The share of chronic odontogenic rhinosinusitis is 40\% among all chronic rhinosinusitis. Using automated information systems for differential diagnosis will improve the efficiency of decision-making by doctors in diagnosing chronic odontogenic rhinosinusitis. Therefore, this study aimed to develop an intelligent decision support system for the differential diagnosis of chronic odontogenic rhinosinusitis based on computer vision methods. A dataset was collected and processed, including 162 MSCT images. A deep learning model for image segmentation was developed. A 23 convolutional layer U-Net network architecture has been used for the segmentation of multi-spiral computed tomography (MSCT) data with odontogenic maxillary sinusitis. The proposed model is implemented in such a way that each pair of repeated 3 × 3 convolutions layers is followed by an Exponential Linear Unit instead of a Rectified Linear Unit as an activation function. The model showed an accuracy of 90.09\%. To develop a decision support system, an intelligent chatbot allows the user to conduct an automated patient survey and collect patient examination data from several doctors of various profiles. The intelligent information system proposed in this study made it possible to combine an image processing model with a patient interview and examination data, improving physician decision-making efficiency in the differential diagnosis of Chronic Odontogenic Rhinosinusitis. The proposed solution is the first comprehensive solution in this area.}, language = {en} } @article{HenningWagnerGedatetal.2023, author = {Henning, Thorsten and Wagner, Philipp and Gedat, Egbert and Kochlik, Bastian and Kusch, Paula and Sowoidnich, Kay and Vastag, Marko and Gleim, Jeannine and Braune, Marcel and Maiwald, Martin and Sumpf, Bernd and Grune, Tilman and Weber, Daniela}, title = {Evaluation of Modern Approaches for the Assessment of Dietary Carotenoids as Markers for Fruit and Vegetable Consumption}, series = {Nutrients}, volume = {15}, journal = {Nutrients}, number = {7}, publisher = {MDPI}, issn = {2072-6643}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17280}, year = {2023}, abstract = {The assessment of dietary carotenoids via blood measurements has been widely used as a marker for fruit and vegetable consumption. In the present study, modern, non-invasive approaches to assess dietary carotenoids, such as skin measurements and an app-based short dietary record (ASDR), were compared with conventional methods such as plasma status and handwritten 3-day dietary records. In an 8-week observational study, 21 healthy participants aged 50-65 years recorded their daily consumption of carotenoid-rich fruits and vegetables via a specially developed ASDR. Anthropometry, blood samplings and assessment of skin carotenoids via Raman and reflection spectroscopy were performed at baseline, after four weeks and at the end of the study. App-based intake data showed good correlations with plasma α-carotene (r = 0.74, p < 0.0001), β-carotene (r = 0.71, p < 0.0001), and total plasma carotenoids (r = 0.65, p < 0.0001); weak correlations with plasma lutein/zeaxanthin and β-cryptoxanthin (both r = 0.34, p < 0.05); and no correlation with plasma lycopene. Skin measurements via reflection and Raman spectroscopy correlated well with total plasma carotenoids (r = 0.81 and 0.72, respectively; both p < 0.0001), α-carotene (r = 0.75-0.62, p < 0.0001), and β-carotene (r = 0.79-0.71, p < 0.0001); moderately with plasma lutein/zeaxanthin (both r = 0.51, p < 0.0001); weakly with plasma β-cryptoxanthin (r = 0.40-0.31, p < 0.05); and showed no correlation with plasma lycopene. Skin measurements could provide a more convenient and noninvasive approach of estimating a person's fruit and vegetable consumption compared to traditional methods, especially in studies that do not intend blood sampling. ASDR records might function as a suitable, convenient tool for dietary assessment in nutritional intervention studies.}, language = {en} } @article{ChumachenkoButkevychLodeetal.2022, author = {Chumachenko, Dmytro and Butkevych, Mykola and Lode, Daniel and Frohme, Marcus and Schmailzl, Kurt J. G. and Nechyporenko, Alina}, title = {Machine Learning Methods in Predicting Patients with Suspected Myocardial Infarction Based on Short-Time HRV Data}, series = {Sensors}, volume = {22}, journal = {Sensors}, number = {18}, publisher = {MDPI}, issn = {1424-8220}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16521}, year = {2022}, abstract = {Diagnosis of cardiovascular diseases is an urgent task because they are the main cause of death for 32\% of the world's population. Particularly relevant are automated diagnostics using machine learning methods in the digitalization of healthcare and introduction of personalized medicine in healthcare institutions, including at the individual level when designing smart houses. Therefore, this study aims to analyze short 10-s electrocardiogram measurements taken from 12 leads. In addition, the task is to classify patients with suspected myocardial infarction using machine learning methods. We have developed four models based on the k-nearest neighbor classifier, radial basis function, decision tree, and random forest to do this. An analysis of time parameters showed that the most significant parameters for diagnosing myocardial infraction are SDNN, BPM, and IBI. An experimental investigation was conducted on the data of the open PTB-XL dataset for patients with suspected myocardial infarction. The results showed that, according to the parameters of the short ECG, it is possible to classify patients with a suspected myocardial infraction as sick and healthy with high accuracy. The optimized Random Forest model showed the best performance with an accuracy of 99.63\%, and a root mean absolute error is less than 0.004. The proposed novel approach can be used for patients who do not have other indicators of heart attacks.}, language = {en} } @article{ArangoPerez2019, author = {Arango P{\´e}rez, Andr{\´e}s}, title = {Implementing Augmented Reality in the Flight Deck for Single Pilot Operations}, series = {Anwendungen und Konzepte der Wirtschaftsinformatik}, volume = {9}, journal = {Anwendungen und Konzepte der Wirtschaftsinformatik}, publisher = {Arbeitskreis Wirtschaftsinformatik (AKWI)}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16649}, pages = {60 -- 72}, year = {2019}, abstract = {Single Pilot Operations is a current topic with the potential to significantly affect the future of commercial aviation. While financially attractive for airlines, Single Pilot Operations bring forth important safety concerns, especially regarding the lack of human redundancy in the flight deck, an increased workload for the single pilot, reduced situational awareness and a higher risk of human error. It is assumed that potential problems affecting Single Pilot Operations could be addressed by implementing an Augmented Reality (AR) device in the flight deck, by presenting additional information and supporting hints within the pilot's field of view. Concretely, AR could be used to help reduce the single pilot's workload, improve situational awareness and reduce the risk of human error. This paper sets out to demonstrate two use cases for augmented reality in the flight deck. A system, called Pilot Assist, was developed that allows pilots to conduct checklists interactively with a Microsoft HoloLens. The system also provides a holographic Head-up-Display. Pilot Assist was developed and demonstrated with a fixed base Airbus A320 simulator at the Technical University of Wildau. With the HoloLens' spatial mapping capabilities - scanning and recognizing the environment around the user - it was possible to create a system that guides the pilot through the conduction of checklists. This is done by prompting the user towards the location of each checklist item in the cockpit, where information regarding necessary actions is projected. Furthermore, Pilot Assist is integrated with the aircraft systems, making it possible to obtain aircraft status data in real time, thus allowing error-checking of the pilot's actions as well as automating the progress through checklists. The holographic Head-up-Display allows the user to look at the surrounding environment while presenting critical flight data within the user's field of view. The holographic Head-up-Display is intended to contribute to the pilot's situational awareness. Experts in the aviation field, including pilots, researchers and engineers had the chance to qualitatively assess the Pilot Assist tool. They pointed to limitations of both Pilot Assist and the HoloLens itself, but shared optimism as to how this technology and similar applications could indeed impact the future of flight operations. Concerns regarding the HoloLens' weight, comfort and narrow field of view were expressed. However, continued development of head mounted devices (e.g. HoloLens 2) is expected in the coming years. Further research into augmented reality applications in the flight deck is needed to advance this and other use cases. Nonetheless, the experts agreed Pilot Assist provides beneficial support during single pilot operation considering the current prototypical nature of the system.}, language = {en} } @article{PeuschkeBischof2019, author = {Peuschke-Bischof, Tobias}, title = {Der Mensch als Einflussfaktor beim manuellen Fahren}, series = {Anwendungen und Konzepte der Wirtschaftsinformatik}, volume = {9}, journal = {Anwendungen und Konzepte der Wirtschaftsinformatik}, publisher = {Arbeitskreis Wirtschaftsinformatik (AKWI)}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16655}, pages = {83 -- 87}, year = {2019}, abstract = {Dieser Ausschnitt aus einer Masterarbeit dient der Betrachtung der Aufnahme und Verarbeitung von Informationen durch den Menschen im Straßenverkehr. Dazu werden unter anderem die Prozesse der Informationsaufnahme, Informationsverarbeitung und die {\"U}berf{\"u}hrung der Informationen in eine Handlung genauer betrachtet, um erkennen zu k{\"o}nnen, welchen Nutzen Fahrerassistenzsysteme erzielen k{\"o}nnen, indem sie dem Menschen die Fahraufgabe entweder teilweise oder sogar vollst{\"a}ndig abnehmen.}, language = {de} } @article{MaiMaiSteglich2022, author = {Mai, Andreas and Mai, Christian and Steglich, Patrick}, title = {From Lab-on-chip to Lab-in-App: Challenges towards silicon photonic biosensors product developments}, series = {Results in Optics}, volume = {9}, journal = {Results in Optics}, publisher = {Elsevier}, issn = {2666-9501}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16687}, year = {2022}, abstract = {This work presents and evaluates different approaches of integrated optical sensors based on photonic integrated circuit (PIC) technologies for refractive index sensing. Bottlenecks in the fabrication flow towards an applicable system are discussed that hinder a cost-effective mass-production for disposable sensor chips. As sensor device, a waveguide coupled micro-ring based approach is chosen which is manufactured in an 8" wafer level process. We will show that the co-integration with a reproducible, scalable and low-cost microfluidic interface is the main challenge which needs to be overcome for future application of silicon technology based PIC sensor chips.}, language = {en} } @article{SiegmannMelzerHeinrich2017, author = {Siegmann, J{\"u}rgen and Melzer, Klaus-Martin and Heinrich, Ingo}, title = {Planbarer Umgang mit der außerplanm{\"a}ßigen Instandhaltung von Eisenbahnfahrzeugen - Paradoxon oder Chance?}, series = {Eisenbahntechnische Rundschau (ETR)}, volume = {66}, journal = {Eisenbahntechnische Rundschau (ETR)}, number = {12}, publisher = {DVV Media Group}, issn = {0013-2845}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16699}, pages = {55 -- 63}, year = {2017}, abstract = {Wettbewerb pr{\"a}gt den liberalisierten Eisenbahnmarkt in Europa und macht unternehmerisches Denken und Handeln der Eisenbahnverkehrsunternehmen im Fern-, Nah- und G{\"u}terverkehr erforderlich. Dabei ist der Umgang mit der Fahrzeuginstandhaltung ein wichtiger Erfolgsfaktor. Der vorliegende Artikel fokussiert sich auf die außerplanm{\"a}ßige Instandhaltung und beschreibt die methodische Herleitung eines mathematischen Modells um den Sch{\"a}digungszustand, also den Grad der Sch{\"a}digung einer Flotte von Eisenbahnfahrzeugen, in eine valide Korrelation mit vorhandenen betrieblichen Ressourcen zu bringen. Diese Wechselbeziehung wird in eine allgemeing{\"u}ltige Kennzahl, dem Schadindex {\"u}berf{\"u}hrt und mittels eines geeigneten Prognosemodells in seinem zeitlichen Verlauf vorhersagbar dargestellt. Die Prognose ber{\"u}cksichtigt auch die M{\"o}glichkeit zur iterativen Anpassung der betrieblichen Ressourcen. So kann beispielsweise das zuk{\"u}nftige Sch{\"a}digungsverhalten einer Eisenbahnfahrzeugflotte in Abh{\"a}ngigkeit einer ver{\"a}nderten Fahrzeugzahl oder einer Umstellung der Mitarbeiterbesetztzeiten in den Instandhaltungswerken simuliert werden. Mit einer systematischen Anwendung des Schadindex erhalten Eisenbahnverkehrsunternehmen mehr Handlungsspielr{\"a}ume zur Verfolgung ihrer strategischen und wirtschaftlichen Ziele. Aus einem Reagieren auf unerwartete Fahrzeugausf{\"a}lle, wird ein Agieren bereits im Vorfeld dieser Ausf{\"a}lle.}, language = {de} } @article{Liebchen2017, author = {Liebchen, Christian}, title = {Quo vadis Taktfahrplanoptimierung im Eisenbahnverkehr?}, series = {Eisenbahntechnische Rundschau (ETR)}, volume = {66}, journal = {Eisenbahntechnische Rundschau (ETR)}, number = {1+2}, publisher = {DVV Media Group}, issn = {0013-2845}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16701}, pages = {31 -- 35}, year = {2017}, abstract = {Von 2005 bis 2008 war es gelungen, f{\"u}r mehrere Netze des Schienenpersonenverkehrs durch den Einsatz mathematischer Optimierungsverfahren Verbesserungen in den Fahrpl{\"a}nen zu erzielen. Seither stehen weitere solche Eins{\"a}tze aus. Der vorliegende Beitrag reflektiert daher, ob die eingesetzten Methoden und nicht zuletzt ggf. sogar die Zielgruppe(n) einer Justierung bed{\"u}rfen.}, language = {de} } @article{KebdaniLehnertMichael2021, author = {Kebdani, Zaki and Lehnert, Martin and Michael, Sabrina}, title = {Hygienisch sicher reisen? - Der {\"o}ffentliche Verkehr in der Pandemie -}, series = {Eisenbahntechnische Rundschau (ETR)}, volume = {2021}, journal = {Eisenbahntechnische Rundschau (ETR)}, number = {9}, publisher = {DVV Media Group}, issn = {0013-2845}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16716}, pages = {16 -- 21}, year = {2021}, abstract = {Entsprechend aktueller wissenschaftlicher Studien ist das Risiko, sich mit SARS-CoV-2 im {\"o}ffentlichen Verkehr zu infizieren, nicht h{\"o}her als im h{\"a}uslichen Umfeld. Besonders in Verkehrsmittelinnenr{\"a}umen kann durch eine ausreichende Frischluftzufuhr, Umluftfilterung, Einhaltung von Abst{\"a}nden und dem richtigen Tragen von geeigneten Schutzmasken das Ansteckungsrisiko effektiv reduziert werden.}, language = {de} } @article{OsdobaLehnert2022, author = {Osdoba, Anne-Katrin and Lehnert, Martin}, title = {Welche Erwartungen hat die Eisenbahnbranche an neueingestellte Mitarbeitende?}, series = {Eisenbahntechnische Rundschau (ETR)}, volume = {2022}, journal = {Eisenbahntechnische Rundschau (ETR)}, number = {4}, publisher = {DVV Media Group}, issn = {0013-2845}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16725}, pages = {23 -- 27}, year = {2022}, abstract = {Dieser Fragestellung geht das EU-Projekt ASTONRail in einem ersten Projektteil nach. In einer Umfrage wurde die Eisenbahnbranche in Deutschland und Europa hinsichtlich ihrer Erwartungen an die Hochschulausbildung neueingestellter Eisenbahningenieurinnen und -ingenieure befragt. Erkenntnisse und Schlussfolgerungen f{\"u}r die Hochschulausbildung werden nachfolgend vorgestellt.}, language = {de} } @article{GrebinykPrylutskaGrebinyketal.2022, author = {Grebinyk, Anna and Prylutska, Svitlana and Grebinyk, Sergii and Ponomarenko, Stanislav and Virych, Pavlo and Chumachenko, Vasyl and Kutsevol, Nataliya and Prylutskyy, Yuriy and Ritter, Uwe and Frohme, Marcus}, title = {Drug delivery with a pH-sensitive star-like dextran-graft polyacrylamide copolymer}, series = {Nanoscale Advances}, volume = {4}, journal = {Nanoscale Advances}, number = {23}, publisher = {Royal Society of Chemistry (RSC)}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16749}, pages = {5077 -- 5088}, year = {2022}, abstract = {The development of precision cancer medicine relies on novel formulation strategies for targeted drug delivery to increase the therapeutic outcome. Biocompatible polymer nanoparticles, namely dextran-graft-polyacrylamide (D-g-PAA) copolymers, represent one of the innovative non-invasive approaches for drug delivery applications in cancer therapy. In this study, the star-like D-g-PAA copolymer in anionic form (D-g-PAAan) was developed for pH-triggered targeted drug delivery of the common chemotherapeutic drugs - doxorubicin (Dox) and cisplatin (Cis). The initial D-g-PAA copolymer was synthesized by the radical graft polymerization method, and then alkaline-hydrolyzed to get this polymer in anionic form for further use for drug encapsulation. The acidification of the buffer promoted the release of loaded drugs. D-g-PAAan nanoparticles increased the toxic potential of the drugs against human and mouse lung carcinoma cells (A549 and LLC), but not against normal human lung cells (HEL299). The drug-loaded D-g-PAAan-nanoparticles promoted further oxidative stress and apoptosis induction in LLC cells. D-g-PAAan-nanoparticles improved Dox accumulation and drugs' toxicity in a 3D LLC multi-cellular spheroid model. The data obtained indicate that the strategy of chemotherapeutic drug encapsulation within the branched D-g-PAAan nanoparticle allows not only to realize pH-triggered drug release but also to potentiate its cytotoxic, prooxidant and proapoptotic effects against lung carcinoma cells.}, language = {en} } @article{MorlockSubramanianZounietal.2022, author = {Morlock, Sascha and Subramanian, Senthil Kumar and Zouni, Athina and Lisdat, Fred}, title = {Bio-inorganic hybrid structures for direct electron transfer to photosystem I in photobioelectrodes}, series = {Biosensors and Bioelectronics}, volume = {204}, journal = {Biosensors and Bioelectronics}, publisher = {Elsevier}, issn = {1873-4235}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16770}, year = {2022}, abstract = {Synthetic materials can be combined with biological components in many ways. One example that provides scientists with multiple challenges is a photobioelectrode that converts sunlight into electrons in a biohybrid approach. In the present study several key parameters are evaluated concerning their influence on the direct electron transfer from a 3D indium tin oxide (ITO) electrode material to photosystem I (PSI) as a light-harvesting biomolecule. In contrast to previous investigations, no mediating molecule is added to shuttle the electrons to the luminal side of PSI. Thus, this setup is less complex than foregoing ones. The solution composition drastically influences the interaction of PSI with the ITO surface. Here, the application of higher buffer concentrations and the addition of salts are advantageous, whereas the nature of the buffer ions plays a minor role. The artificial electrode material's thickness is adjustable since a spin-coating procedure is used for preparation. With a 30 μm thick structure and immobilized PSI cathodic photocurrents up to 10.1 μA cm-2 are obtained at 100 mW cm-2 illumination intensity and an applied potential of -0.1V vs. Ag/AgCl. Over a period of three days the photobioelectrodes are illuminated for a total of 90 min and stored between the measurements at ambient temperature. The stability of the setup is noteworthy as still about 90\% of the photocurrent is retained. The photocathode described here offers many positive features, including a high onset potential for the photocurrent starting sligthly above the redox potentail of P700, and applicability in a wide pH range from pH 5 to 8.}, language = {en} } @article{HagenYoonZhangetal.2022, author = {Hagen, Dirk J. and Yoon, Jiho and Zhang, Haojie and Kalkofen, Bodo and Silinskas, Mindaugas and B{\"o}rrnert, Felix and Han, Hyeon and Parkin, Stuart S. P.}, title = {Atomic Layer Deposition of the Conductive Delafossite PtCoO2}, series = {Advanced Materials Interfaces}, volume = {9}, journal = {Advanced Materials Interfaces}, number = {12}, publisher = {Wiley}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16076}, year = {2022}, abstract = {The first atomic layer deposition process for a ternary oxide is reported, which contains a metal of the platinum group, the delafossite PtCoO2. The deposition with the precursors trimethyl-Pt-methylcyclopentadienyl, Co-bis(N-t-butyl-N′-ethylpropanimidamidate), and oxygen plasma results in a process with a nearly constant growth rate and stoichiometric composition over a wide temperature window from 100 to 320 °C. Annealing of the as-deposited amorphous films in an oxygen atmosphere in a temperature window from 700 to 800 °C leads to the formation of the delafossite phase. Very thin films show a pronounced preferred orientation with the Pt sheets being almost parallel to the substrate surface while arbitrary orientation is observed for thicker films. The conformal coating of narrow trenches highlights the potential of this atomic-layer-deposition process. Moreover, heterostructures with magnetic films are fabricated to demonstrate the potential of PtCoO2 for spintronic applications.}, language = {en} } @inproceedings{ElGhayedFalkWilbersetal.2023, author = {El Ghayed, Tarek and Falk, Constantin and Wilbers, Simon and van de Sand, Ron and Reiff-Stephan, J{\"o}rg}, title = {Modellansatz zur Prozessoptimierung beim hydroadh{\"a}siven Greifen}, series = {Tagungsband AALE 2023 : Mit Automatisierungstechnik gegen den Klimawandel}, booktitle = {Tagungsband AALE 2023 : Mit Automatisierungstechnik gegen den Klimawandel}, publisher = {Hochschule f{\"u}r Technik, Wirtschaft und Kultur}, address = {Leipzig}, isbn = {978-3-910103-01-6}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-17150}, pages = {1 -- 10}, year = {2023}, abstract = {Der Produktlebenszyklus von Kleidung ist vor allem durch lange Lieferketten von Niedriglohnl{\"a}ndern in Hochlohnl{\"a}nder gekennzeichnet. Die Eliminierung solcher Transportwege k{\"o}nnte dazu f{\"u}hren, dass der komplette Lebenszyklus von Kleidungsware nachhaltiger und umweltfreundlicher wird. Der Aufbau von Produktionsst{\"a}tten in Hochlohnl{\"a}ndern wird aktuell durch technische Herausforderungen des Produktionsprozesses von Kleidung bzw. allgemein biegeschlaffen Materialien verhindert. Aufgrund der Eigenschaften biegeschlaffer Materialien k{\"o}nnen die Entnahme bzw. definierte Ablage von Textilteilen heutzutage nur teilautomatisiert bzw. mit einem hohen Anteil manueller T{\"a}tigkeiten gel{\"o}st werden. Das Hydroadh{\"a}sive Greifen k{\"o}nnte eine L{\"o}sung sein, die oben genannten Prozesse zu automatisieren und die Produktion f{\"u}r Hochlohnl{\"a}nder zu verg{\"u}nstigen. Jedoch verf{\"u}gt das Verfahren noch {\"u}ber lange Zykluszeiten und die Haltekr{\"a}fte sind nicht reproduzierbar. Weiterhin ist die Findung von Einstellparametern sehr zeitaufwendig und komplex. Die L{\"o}sung k{\"o}nnte eine intelligente Steuerung sein, dessen Kernst{\"u}ck ein datengetriebenes Modell f{\"u}r die Parameteroptimierung und -findung ist. Im Rahmen der Forschungsarbeiten wird ein Modellansatz zur Optimierung der Taktzeit beim hydroadh{\"a}siven Greifen erarbeitet. Dabei wird die Gewinnung der Datengrundlage, das zu entwickelnde Modell sowie die eigentliche Validierung diskutiert.}, language = {de} } @article{KoberSchaeferHollertetal.2023, author = {Kober, Liane and Schaefer, Paul and Hollert, Henner and Frohme, Marcus}, title = {A novel strategy for high-throughput sample collection, analysis and visualization of explosives' concentrations for contaminated areas}, series = {International Journal of Environmental Science and Technology}, volume = {20}, journal = {International Journal of Environmental Science and Technology}, number = {2}, publisher = {Springer Nature}, issn = {1735-2630}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15981}, pages = {1399 -- 1410}, year = {2023}, abstract = {The use of explosives has led to a widespread distribution of 2,4,6-trinitrotoluene (TNT) and its by- and degradation products in the soil on former production and testing sites. The investigation of those large contaminated sites is so far based on a few selected soil samples, due to high costs of conventional HPLC and GC analysis, although huge differences in concentrations can already be found in small areas and different collection depths. We introduce a novel high-throughput screening system for those areas, which combines a smartphone-based collection of GPS data and soil characteristics with a fast MALDI-TOF MS quantification of explosives in soil sample extracts and finally a heatmap visualization of the explosives' spread in soil and an analysis of correlation between concentrations and soil characteristics. The analysis of a 400 m2 area presented an extensive contamination with TNT and lower concentrations of the degradation and by-products aminodinitrotoluenes (ADNT) and dinitrotoluenes (DNT) next to a former production facility for TNT. The contamination decreased in deeper soil levels and depended on the soil type. Pure humus samples showed significantly lower contaminations compared to sand and humus/sand mixtures, which is likely to be caused by an increased binding potential of the humic material. No correlation was found between the vegetation and the concentration of explosives. Since the results were obtained and visualized within several hours, the MALDI-TOF MS based comprehensive screening and heatmap analysis might be valuable for a fast and high-throughput characterization of contaminated areas.}, language = {en} } @article{SteglichSchasfoort2022, author = {Steglich, Patrick and Schasfoort, Richard B. M.}, title = {Surface Plasmon Resonance Imaging (SPRi) and Photonic Integrated Circuits (PIC) for COVID-19 Severity Monitoring}, series = {COVID}, volume = {2}, journal = {COVID}, number = {3}, publisher = {MDPI}, issn = {2673-8112}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16004}, pages = {389 -- 397}, year = {2022}, abstract = {Direct optical detection methods such as surface plasmon resonance imaging (SPRi) and photonic-integrated-circuits (PIC)-based biosensors provide a fast label-free detection of COVID-19 antibodies in real-time. Each technology, i.e., SPRi and PIC, has advantages and disadvantages in terms of throughput, miniaturization, multiplexing, system integration, and cost-effective mass production. However, both technologies share similarities in terms of sensing mechanism and both can be used as high-content diagnostics at or near to point of care, where the analyte is not just quantified but comprehensively characterized. This is significant because recent results suggest that not only the antibody concentration of the three isotypes IgM, IgG, and IgA but also the strength of binding (affinity) gives an indication of potential COVID-19 severity. COVID-19 patients with high titers of low affinity antibodies are associated with disease severity. In this perspective, we provide some insights into how SPR and PIC technologies can be effectively combined and complementarily used for a comprehensive COVID-19 severity monitoring. This opens a route toward an immediate therapy decision to provide patients a treatment in an early stage of the infection, which could drastically lowers the risk of a severe disease course.}, language = {en} } @article{SteglichLecciMai2022, author = {Steglich, Patrick and Lecci, Giulia and Mai, Andreas}, title = {Surface Plasmon Resonance (SPR) Spectroscopy and Photonic Integrated Circuit (PIC) Biosensors: A Comparative Review}, series = {Sensors}, volume = {22}, journal = {Sensors}, number = {8}, publisher = {MDPI}, issn = {1424-8220}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16018}, year = {2022}, abstract = {Label-free direct-optical biosensors such as surface-plasmon resonance (SPR) spectroscopy has become a gold standard in biochemical analytics in centralized laboratories. Biosensors based on photonic integrated circuits (PIC) are based on the same physical sensing mechanism: evanescent field sensing. PIC-based biosensors can play an important role in healthcare, especially for point-of-care diagnostics, if challenges for a transfer from research laboratory to industrial applications can be overcome. Research is at this threshold, which presents a great opportunity for innovative on-site analyses in the health and environmental sectors. A deeper understanding of the innovative PIC technology is possible by comparing it with the well-established SPR spectroscopy. In this work, we shortly introduce both technologies and reveal similarities and differences. Further, we review some latest advances and compare both technologies in terms of surface functionalization and sensor performance.}, language = {en} } @article{LindnerLiebchen2022, author = {Lindner, Niels and Liebchen, Christian}, title = {Timetable merging for the Periodic Event Scheduling Problem}, series = {EURO Journal on Transportation and Logistics}, volume = {11}, journal = {EURO Journal on Transportation and Logistics}, publisher = {Elsevier}, issn = {2192-4376}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-16054}, year = {2022}, abstract = {We propose a new mixed integer programming based heuristic for computing new benchmark primal solutions for instances of the PESPlib. The PESPlib is a collection of instances for the Periodic Event Scheduling Problem (PESP), comprising periodic timetabling problems inspired by real-world railway timetabling settings, and attracting several international research teams during the last years. We describe two strategies to merge a set of good periodic timetables. These make use of the instance structure and minimum weight cycle bases, finally leading to restricted mixed integer programming formulations with tighter variable bounds. Implementing this timetable merging approach in a concurrent solver, we improve the objective values of the best known solutions for the smallest and largest PESPlib instances by 1.7 and 4.3 percent, respectively.}, language = {en} } @article{DeRocchisRoitschFranken2022, author = {De Rocchis, Vincenzo and Roitsch, Thomas and Franken, Philipp}, title = {Extracellular Glycolytic Activities in Root Endophytic Serendipitaceae and Their Regulation by Plant Sugars}, series = {Microorganisms}, volume = {10}, journal = {Microorganisms}, number = {2}, editor = {Strobel, Gary A.}, publisher = {MDPI}, issn = {2076-2607}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15774}, year = {2022}, abstract = {Endophytic fungi that colonize the plant root live in an environment with relative high concentrations of different sugars. Analyses of genome sequences indicate that such endophytes can secrete carbohydrate-related enzymes to compete for these sugars with the surrounding plant cells. We hypothesized that typical plant sugars can be used as carbon source by root endophytes and that these sugars also serve as signals to induce the expression and secretion of glycolytic enzymes. The plant-growth-promoting endophytes Serendipita indica and Serendipita herbamans were selected to first determine which sugars promote their growth and biomass formation. Secondly, particular sugars were added to liquid cultures of the fungi to induce intracellular and extracellular enzymatic activities which were measured in mycelia and culture supernatants. The results showed that both fungi cannot feed on melibiose and lactose, but instead use glucose, fructose, sucrose, mannose, arabinose, galactose and xylose as carbohydrate sources. These sugars regulated the cytoplasmic activity of glycolytic enzymes and also their secretion. The levels of induction or repression depended on the type of sugars added to the cultures and differed between the two fungi. Since no conventional signal peptide could be detected in most of the genome sequences encoding the glycolytic enzymes, a non-conventional protein secretory pathway is assumed. The results of the study suggest that root endophytic fungi translocate glycolytic activities into the root, and this process is regulated by the availability of particular plant sugars.}, language = {en} } @article{PfeilNechyporenkoFrohmeetal.2022, author = {Pfeil, Juliane and Nechyporenko, Alina and Frohme, Marcus and Hufert, Frank T. and Schulze, Katja}, title = {Examination of blood samples using deep learning and mobile microscopy}, series = {BMC Bioinformatics}, volume = {23}, journal = {BMC Bioinformatics}, publisher = {BioMed Central}, issn = {1471-2105}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15802}, year = {2022}, abstract = {Microscopic examination of human blood samples is an excellent opportunity to assess general health status and diagnose diseases. Conventional blood tests are performed in medical laboratories by specialized professionals and are time and labor intensive. The development of a point-of-care system based on a mobile microscope and powerful algorithms would be beneficial for providing care directly at the patient's bedside. For this purpose human blood samples were visualized using a low-cost mobile microscope, an ocular camera and a smartphone. Training and optimisation of different deep learning methods for instance segmentation are used to detect and count the different blood cells. The accuracy of the results is assessed using quantitative and qualitative evaluation standards.}, language = {en} } @article{HerkenhoffBovolentaBroedeletal.2021, author = {Herkenhoff, Marcos Edgar and Bovolenta, Luiz A. and Br{\"o}del, Oliver and dos Santos, Lucilene Delazari and de Oliveira, Arthur C. and Chuffa, Luiz G.A. and Ribeiro, Amanda O. and Lupi, Luiz A. and Dias, Marco A.D. and Hilsdorf, Alexandre W.S. and Frohme, Marcus and Pinhal, Danillo}, title = {Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus)}, series = {Genomics}, volume = {113}, journal = {Genomics}, number = {6}, publisher = {Elsevier}, issn = {0888-7543}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15571}, pages = {4303 -- 4312}, year = {2021}, abstract = {Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed.}, language = {en} } @article{WielandKuhlsLentzetal.2021, author = {Wieland, Ralf and Kuhls, Katrin and Lentz, Hartmut H.K. and Conraths, Franz and Kampen, Helge and Werner, Doreen}, title = {Combined climate and regional mosquito habitat model based on machine learning}, series = {Ecological Modelling}, volume = {452}, journal = {Ecological Modelling}, address = {Elsevier}, issn = {0304-3800}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15649}, year = {2021}, abstract = {Besides invasive mosquito species also several native species are proven or suspected vectors of arboviruses as West Nile or Usutu virus in Western Europe. Habitat models of these native vectors can be a helpful tool for assessing the risk of autochthonous occurrence, outbreaks and spread of diseases caused by such arboviruses. Modelling native mosquitoes is complicated because of the perfect adaptation to the climatic and landscape conditions and their high abundance in contrast to invasive species. Here we present a new approach for such a habitat model for native mosquito species in Germany, which are considered as vectors of West Nile virus (WNV). Epizootic emergence of WNV was registered in Germany since 2018. The models are based on surveillance data of mosquitoes from the German citizen science project "M{\"u}ckenatlas" complemented by data from systematic trap monitoring in Germany, and on data freely available from the Deutscher Wetterdienst (DWD) and OpenStreetMap (OSM). While climatic factors still play an important role, we could show that habitat suitability is predictable only by the combination of the climate model with a regional model. Both models were based on a machine-learning approach using XGBoost. Evaluation of the accuracy of the models was done by statistical analysis, determining among others feature importances using the SHAP-Library. Final output of the combined climatic and regional models are maps showing the superposed habitat suitability which are generated through a number of steps described in detail. These maps also include the registered cases of WNV infections in the selected region of Germany.}, language = {en} } @article{GerhardDobis2021, author = {Gerhard, Christoph and Dobis, Alexander}, title = {Manufacturing-induced surface contaminations}, series = {Vakuum in Forschung und Praxis}, volume = {33}, journal = {Vakuum in Forschung und Praxis}, number = {1}, publisher = {Wiley}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15656}, pages = {33 -- 37}, year = {2021}, abstract = {Summary In the course of classical optics manufacturing glass components are in permanent direct contact with aqueous operating materials. Such contact leads to a certain absorption of water and hydrogenous compounds that may induce severe glass defects. In this contribution, absorption of hydrogen and other contaminants during grinding of glass was observed and qualitatively detected via laser-induced breakdown spectroscopy. It is shown that hydrogen, calcium, magnesium, and carbon are implanted into the glass surface where the contaminant concentration increases over grinding time or contact time of the glass surface with water and the grinding tool, respectively. The contaminants hydrogen, calcium, and magnesium can be attributed to the water used as lubricant. In contrast, carbon most likely originates from wear debris of the used silicon carbide grinding pads. Several possible mechanisms that lead to such surface contamination of glasses during grinding - diffusion, accumulation in micro cracks as well as the formation of hydrated silica - are finally introduced.}, language = {en} } @article{BauerFursenkoHeinrichetal.2022, author = {Bauer, Joachim and Fursenko, Oksana and Heinrich, Friedhelm and Gutke, Marko and Kornejew, Eckhart and Br{\"o}del, Oliver and Dietzel, Birgit and Kaltenbach, Alexander and Burkhardt, Martin and Edling, Matthias and Steglich, Patrick and Herzog, Michael and Schrader, Sigurd}, title = {Determination of optical constants and scattering properties of transparent polymers for use in optoelectronics}, series = {Optical Materials Express}, volume = {12}, journal = {Optical Materials Express}, number = {1}, publisher = {Optica Publishing Group}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15666}, pages = {204 -- 224}, year = {2022}, abstract = {Knowledge of optical constants, i.e. refractive index n and extinction coefficient k, and light scattering properties of optical polymers are required to optimize micro-optics for light-emitting diodes in terms of efficiency, color properties and light distribution. We present here a model-based diagnostic approach to determine the optical properties of polymers, which should be particularly useful in the development of plastics for optical applications. Optical constants and scattering coefficients were obtained from transmission and reflection measurements in a wavelength range from UV to NIR taking into account scattering effects due to rough surfaces and volume inhomogeneity. Based on the models for the dielectric function, the molecular optical transition energies Eg, critical point energies, Urbach energies and exciton transition energies were determined. Rayleigh and Mie scattering model and van de Hulst\&\#x0027;s anomalous diffraction theory were applied to characterize scattering due to volume inhomogeneities. Scalar diffraction theory was applied to account for surface roughness scattering. Atomic force microscopy with nanomechanical characterization was used to characterize domains in size and shape and to assign optical scattering to a suitable morphological model. The combined optical and mechanical characterization help to improve the qualification of new polymer materials for optical applications.}, language = {en} } @article{DrostMarschmeyerFraschkeetal.2022, author = {Drost, Martin and Marschmeyer, Steffen and Fraschke, Mirko and Fursenko, Oksana and B{\"a}rwolf, Florian and Costina, Ioan and Kalishettyhalli Mahadevaiah, Mamathamba and Lisker, Marco}, title = {Etch mechanism of an Al₂O₃ hard mask in the Bosch process}, series = {Micro and Nano Engineering}, volume = {14}, journal = {Micro and Nano Engineering}, publisher = {Elsevier}, issn = {2590-0072}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15678}, year = {2022}, abstract = {The etching of high aspect ratio structures in silicon via the Bosch process is essential in modern technologies such as microelectromechanical systems (MEMS) and through‑silicon vias (TSV) fabrication. The process can be very demanding on the mask selectivity due to long etching times, and it has been shown that an Al2O3 hard mask is very suitable in this regard, as it offers significantly higher selectivity compared to the conventional SiO2 or resist masks. In this work, we employ a combination of Scanning Electron Microscopy (SEM), Spectroscopic Ellipsometry (SE) and X-Ray Photoelectron Spectroscopy (XPS) depth profiling to scrutinize the Al2O3 mask etching mechanism and therefore the origin of the extraordinary high selectivity. We demonstrate that by increasing the passivation step time, a thicker fluorocarbon polymer layer is formed on the Al2O3, and Al2O3 is then removed with a minuscule average etch rate of 0.01 nm/min. XPS depth profiling reveals that during Deep Reactive Ion Etching (DRIE) using the Bosch process, an AlFx layer is formed between the polymer and Al2O3. As AlFx is non-volatile, it requires sputtering to be removed. If the polymer layer is thick enough to attenuate the incoming ions such that their energy is not sufficient to lead to desorption of AlFx, such as when using a longer passivation time, the mask is not eroded. By investigating the surface after different amounts of DRIE cycles, we also obtained information about the formation rate of AlFx and the changes in the Al2O3 and polymer thicknesses over the course of a DRIE process. These findings further expand the knowledge of DRIE and can help process engineers to tailor the processes accordingly.}, language = {en} } @article{HeinsohnNiedlAnielskietal.2022, author = {Heinsohn, Natascha Katharina and Niedl, Robert Raimund and Anielski, Alexander and Lisdat, Fred and Beta, Carsten}, title = {Electrophoretic µPAD for Purification and Analysis of DNA Samples}, series = {Biosensors}, volume = {12}, journal = {Biosensors}, number = {2}, publisher = {MDPI}, issn = {2079-6374}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15763}, year = {2022}, abstract = {In this work, the fabrication and characterization of a simple, inexpensive, and effective microfluidic paper analytic device (µPAD) for monitoring DNA samples is reported. The glass microfiber-based chip has been fabricated by a new wax-based transfer-printing technique and an electrode printing process. It is capable of moving DNA effectively in a time-dependent fashion. The nucleic acid sample is not damaged by this process and is accumulated in front of the anode, but not directly on the electrode. Thus, further DNA processing is feasible. The system allows the DNA to be purified by separating it from other components in sample mixtures such as proteins. Furthermore, it is demonstrated that DNA can be moved through several layers of the glass fiber material. This proof of concept will provide the basis for the development of rapid test systems, e.g., for the detection of pathogens in water samples.}, language = {en} }