@article{BoettcherHooglandDitsetal.2015, author = {B{\"o}ttcher, Ren{\´e} and Hoogland, A. Marije and Dits, Natasja and Verhoef, Esther and Kweldam, Charlotte and Waranecki, Piotr and Bangma, Chris H. and van Leenders, Geert J.L.H. and Jenster, Guido}, title = {Novel long non-coding RNAs are specific diagnostic and prognostic markers for prostate cancer}, series = {Oncotarget}, volume = {6}, journal = {Oncotarget}, number = {6}, issn = {1949-2553}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6329}, pages = {4036 -- 4050}, year = {2015}, abstract = {Current prostate cancer (PCa) biomarkers such as PSA are not optimal in distinguishing cancer from benign prostate diseases and predicting disease outcome. To discover additional biomarkers, we investigated PCa-specific expression of novel unannotated transcripts. Using the unique probe design of Affymetrix Human Exon Arrays, we identified 334 candidates (EPCATs), of which 15 were validated by RT-PCR. Combined into a diagnostic panel, 11 EPCATs classified 80\% of PCa samples correctly, while maintaining 100\% specificity. High specificity was confirmed by in situ hybridization for EPCAT4R966 and EPCAT2F176 (SChLAP1) on extensive tissue microarrays. Besides being diagnostic, EPCAT2F176 and EPCAT4R966 showed significant association with pT-stage and were present in PIN lesions. We also found EPCAT2F176 and EPCAT2R709 to be associated with development of metastases and PCa-related death, and EPCAT2F176 to be enriched in lymph node metastases. Functional significance of expression of 9 EPCATs was investigated by siRNA transfection, revealing that knockdown of 5 different EPCATs impaired growth of LNCaP and 22RV1 PCa cells. Only the minority of EPCATs appear to be controlled by androgen receptor or ERG. Although the underlying transcriptional regulation is not fully understood, the novel PCa-associated transcripts are new diagnostic and prognostic markers with functional relevance to prostate cancer growth.}, language = {en} } @misc{RiedelKartchemnikSchoeningetal.2015, author = {Riedel, Marc and Kartchemnik, Julia and Sch{\"o}ning, Michael J. and Lisdat, Fred}, title = {Impedimetrischer DNA Nachweis - Schritte in Richtung sensorischer Anwendung}, series = {Wissenschaftliche Beitr{\"a}ge 2015}, volume = {19}, journal = {Wissenschaftliche Beitr{\"a}ge 2015}, issn = {0949-8214}, doi = {10.15771/0949-8214_2015_1_3}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-3538}, pages = {21 -- 28}, year = {2015}, abstract = {Diese Studie beschreibt einen labelfreien impedimetrischen Sensor auf der Grundlage von kurzen einzelstr{\"a}ngigen DNA-Erkennungselementen f{\"u}r den Nachweis von Hybridisierungsereignissen. Der Fokus der Arbeit liegt auf der Aufkl{\"a}rung des Einflusses der Ziel-DNA-L{\"a}nge und der Erkennungssequenzposition auf die sensorische Leistungsf{\"a}higkeit. Die impedimetrischen Messungen werden in Anwesenheit des Redoxsystems Kaliumhexacyanoferrat (II/III) durchgef{\"u}hrt und zeigen einen Anstieg des Durchtrittswiderstandes nach der Hybridisierung mit komplement{\"a}rer Ziel-DNA mit einer Nachweisgrenze im unteren nanomolaren Bereich. Nach der Hybridisierung kann die Regeneration des Sensors mit deionisiertem Wasser durch die Einstellung effektiver Konvektionsbedingungen erreicht werden und erm{\"o}glicht somit eine Wiederverwendbarkeit des Sensors. Untersuchungen zu l{\"a}ngeren Ziel-DNA-Str{\"a}ngen mit einem zur L{\"o}sung exponierten {\"U}berhang demonstrieren die Anwendbarkeit des impedimetrischen Nachweises f{\"u}r l{\"a}ngere Sequenzen. Allerdings resultiert eine zunehmende {\"U}berhangl{\"a}nge in einer verringerten Durchtrittswiderstands{\"a}nderung. Um die Impedanz{\"a}nderung f{\"u}r l{\"a}ngere Ziel-DNA zu erh{\"o}hen, wird die Erkennungssequenzposition ver{\"a}ndert, sodass ein kleiner {\"U}berhang zur Elektrode ausgerichtet ist. Die Ergebnisse legen nahe, dass DNA in direkter N{\"a}he zur Elektrode einen gr{\"o}ßeren Einfluss auf das impedimetrische Signal besitzt als weiter entfernte DNA.}, language = {de} } @misc{SchneiderPaeschkeAlarconetal.2015, author = {Schneider, Torsten and Paeschke, Robert and Alarcon, Daniel and Blaschke, Peter}, title = {Strukturdynamik- und Maschinendiagnose an einem Cherenkov-Teleskop}, series = {Wissenschaftliche Beitr{\"a}ge 2015}, volume = {19}, journal = {Wissenschaftliche Beitr{\"a}ge 2015}, issn = {0949-8214}, doi = {10.15771/0949-8214_2015_1_9}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-3595}, pages = {67 -- 74}, year = {2015}, abstract = {Das Deutsche Elektronen-Synchrotron (DESY) in Zeuthen entwickelt zurzeit die 12m-Teleskope im Rahmen des internationalen Cherenkov-Telescope-Array (CTA)-Konsortiums. Es ist geplant, die Teleskope {\"u}ber einen Zeitraum von mindestens 30 Jahren kosteng{\"u}nstig zu betreiben. Dabei f{\"u}hrt der autarke Betrieb von Teleskopen an abgelegenen Standorten zu speziellen Anforderungen an die Ausfallsicherheit. Diese Anforderungen gelten sowohl f{\"u}r die Tragstruktur als auch f{\"u}r die Antriebskomponenten. Zustands{\"u}berwachung und Verschleißanalysen sind in diesem Anwendungsfall mit klassischen Methoden der Maschinen- und Struktur{\"u}berwachung nur eingeschr{\"a}nkt m{\"o}glich. Methoden der Mustererkennung aus dem Bereich der Psychoakustik konnten erfolgreich verwendet werden, um den Langsamlauf zu analysieren. In diesem Beitrag werden Ergebnisse von Schwingungsuntersuchungen an einem 1:1-Prototyp des Teleskops vorgestellt. Zus{\"a}tzlich wird die Frage beantwortet, wie ein kosteng{\"u}nstiges {\"U}berwachungssystem mit minimaler Sensoranzahl realisiert werden kann.}, language = {de} } @misc{BezrukGedatHolzheimeretal.2015, author = {Bezruk, Valeriy and Gedat, Egbert and Holzheimer, Katja and Vandenhouten, Ralf}, title = {Automated recognition of sleep stages by electroencephalograms}, series = {Wissenschaftliche Beitr{\"a}ge 2015}, volume = {19}, journal = {Wissenschaftliche Beitr{\"a}ge 2015}, issn = {0949-8214}, doi = {10.15771/0949-8214_2015_1_5}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-3552}, pages = {39 -- 43}, year = {2015}, abstract = {The assessment of different sleep stages and their disorders in diseases is an important part of telematic medicine. With an electroencephalogram, the different stages of sleep can be monitored and classified with respect to brain activity. By means of modern data management such as the patient monitor ixTrend, for example, the data can be recorded for long sleep phases and evaluated by a computer using appropriate software, such as Dataplore. Here, a new mathematical model for the automated classification of sleep stages is introduced. The statistical method of autocorrelation, applied to six known sleep stages, was extended by one new class for unknown signals. Due to this new class, it is not necessary to sort all recorded EEG signals into one of the known classes, thereby, minimising the probability of errors. Further, the dependence of the error probability on the duration of the analysed EEG signal was assessed. A minimal error probability of pmin = 0.15 was detected. Exemplary data for one patient are reported.}, language = {en} } @article{LuxSiemrothSgarlataetal.2015, author = {Lux, Helge and Siemroth, Peter and Sgarlata, Anna and Prosposito, Paolo and Schubert, Markus Andreas and Casalboni, Mauro and Schrader, Sigurd}, title = {Synthesis of graphene-like transparent conductive films on dielectric substrates using a modified filtered vacuum arc system}, series = {Journal of Applied Physics}, volume = {117}, journal = {Journal of Applied Physics}, number = {19}, issn = {1089-7550}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-9738}, year = {2015}, abstract = {Here, we present a reliable process to deposit transparent conductive films on silicon oxide, quartz, and sapphire using a solid carbon source. This layer consists of partially ordered graphene flakes with a lateral dimension of about 5 nm. The process does not require any catalytic metal and exploits a high current arc evaporation (Φ-HCA) to homogeneously deposit a layer of carbon on heated substrates. A gas atmosphere consisting of Argon or Argon/Hydrogen blend acting as a buffer influences the morphology of the growing film. scanning tunneling microscopy, transmission electron microscopy, and Raman spectra were used for a thorough characterization of the samples in order to optimize the growth parameters. The best carbon layers have a surface resistance of 5.7 × 103 Ω◻ whereas the optical transparency of the coatings is 88\% with an excellent homogeneity over areas of several cm2. Such results are compatible with most semiconductor fabrication processes and make this method very promising for various industrial applications.}, language = {en} } @article{KoellingLiskerHellwigetal.2015, author = {K{\"o}lling, Axel and Lisker, Roberto and Hellwig, Udo and Wildenauer, Franz-Xaver}, title = {Friction expander for the generation of electricity (FEGE)}, series = {Renewable Energy and Power Quality Journal (REPQJ)}, volume = {13}, journal = {Renewable Energy and Power Quality Journal (REPQJ)}, number = {4}, publisher = {Cultech Publishing}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-21257}, pages = {489 -- 493}, year = {2015}, abstract = {At present and in future the necessity of generating electricity using renewable resources globally is most important and clearly evident. Depending on how the scenarios are designed and disregarding fossil resources the contributions of renewable energy sources have to be increased by a factor of four to six as far as it concerns the installed capacity and the amount of electricity generated. The corresponding annual investment costs amount some hundred billion US-\$ and the CO₂ emission reduction equals one Gt. In any case heat to electricity conversion systems based on biomass, geothermal and process heat recovery are expected to provide significant contributions to this objective. Besides several others the conversion technology of a Rankine Cycle Process is most suitable over a wide range of capacities notwithstanding the possibilities to applicate working fluids of non-organic or organic character. Particularly for the heat recovery from the exhaust gas of biogas operated internal combustion engines the type of a newly developed friction expander was designed, constructed, built and evaluated. Based on preceding theoretical and numerical calculations as well as constructional and experimental studies using hexane and hexamethoxydisiloxane in the project reported here saturated water steam was used as working fluid. During the experiments carried out in a special test facility the electric power of the system amounted 1.4 kW representing two thirds of the conceptual design.}, language = {en} } @incollection{MietznerVandenhoutenHartmann2015, author = {Mietzner, Dana and Vandenhouten, Ralf and Hartmann, Frank}, title = {Collaborative Problem Solving for Regional Development? - A New Approach of Public Participation}, series = {Electronic Government and Electronic Participation}, booktitle = {Electronic Government and Electronic Participation}, editor = {Tambouris, Efthimios}, publisher = {IOS Press}, address = {Amsterdam}, isbn = {978-1-61499-570-8}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-9177}, publisher = {Technische Hochschule Wildau}, pages = {19 -- 26}, year = {2015}, abstract = {In this paper, we present a concept prototype for a socio-technical assistant (s.TAS) as a collaborative problem-solving approach to support the regional development in a selected rural district. The presented concept considers insides from citizen participation as an open government instrument and strongly incorporates collaborative value creation to address regional development issues. The presented approach incorporates citizens and other regional stakeholders (e.g., companies, organizations, associations) as problem-solvers and strongly enables regional stakeholders and the public administration to develop solutions jointly as an ongoing learning process. In this sense, s.TAS addresses participation approaches and elaborates participation to collaboration. For the development of this participatory problem-solving approach, we conducted (1) a broad literature review on participatory approaches and (2) we implemented a qualitative, collaborative research approach with participants from different stakeholder groups. In several workshop rounds, supported by interviews and surveys we developed a concept prototype for the socio-technical assistant s.TAS, which is a technical, smart system, which supports information, communication, participation and collaboration in the selected region.}, language = {en} } @article{VandenhoutenWendlandt2015, author = {Vandenhouten, Ralf and Wendlandt, Ole}, title = {High-precision Optical Position Measurement in Indoor Environments}, series = {Optik \& Photonik}, volume = {10}, journal = {Optik \& Photonik}, number = {3}, issn = {1863-1460}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-9305}, pages = {24 -- 27}, year = {2015}, abstract = {Indoor localization is still a challenge today. Conventional methods based on satellite navigation, like GPS, can only be used outdoors. On the other hand, radio signal architectures, like WiFi or NFC, require high cost infrastructure to be installed inside the building. In collaboration with Ixellence GmbH, the research group for telematics of the University of Applied Science Wildau is developing a new inexpensive solution based on the camera of a mobile device. This approach only requires the availability of multiple identifiable landmarks that can be installed cost-efficiently in an indoor environment. Our investigations show that this method is very accurate and can produce repeatable, robust results.}, language = {en} } @misc{HeinrichSeydewitzWildenaueretal.2015, author = {Heinrich, Moritz and Seydewitz, Tobias and Wildenauer, Franz-Xaver and Pospisil, Heike}, title = {Bioprozessautomatisierung einer Algenanlage mithilfe eines Single-Board-Computers}, series = {Wissenschaftliche Beitr{\"a}ge 2015}, volume = {19}, journal = {Wissenschaftliche Beitr{\"a}ge 2015}, issn = {0949-8214}, doi = {10.15771/0949-8214_2015_1_1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-3515}, pages = {7 -- 14}, year = {2015}, abstract = {F{\"u}r die effektive Haltung von Mikroorganismen, kleinen Pflanzen oder Algen in Bioreaktoren ist die Aufrechterhaltung optimaler Kultivierungsbedingungen, wie beispielsweise pH-Wert, Temperatur oder N{\"a}hrstoffgehalt, notwendig. Diese Parameter k{\"o}nnen sich w{\"a}hrend der Kultivierung {\"a}ndern, weshalb sie regelm{\"a}ßig kontrolliert und gegebenenfalls angepasst werden m{\"u}ssen. Wir pr{\"a}sentieren hier den technischen Aufbau und die Softwarerealisierung eines Automatisierungssystems zur autonomen Regulierung des pH-Wertes in Bioreaktoren, in denen die gr{\"u}ne Mikroalge Scenedesmus rubescens kultiviert wird. Dazu wurde ein System mit pH-Sensoren, Signalwandlern und Magnetventilen zur kontrollierten CO2-Begasung aufgebaut. F{\"u}r die Steuerung und die Datenaufzeichnung diente ein Single-Board-Computer (Raspberry Pi) mit Webeserver. Die Anlage war voll funktionsf{\"a}hig und konnte {\"u}ber mehrere Tage fehlerlos den pH-Wert auf einen vorgegebenen Wert regeln. Das System ist leicht auch auf Großanlagen und f{\"u}r andere Parameter erweiterbar. Durch die Nutzung eines Single-Board-Computers erfordert die Anlage nur minimalen Platz- und Energiebedarf und ist mit geringen Anschaffungskosten verbunden.}, language = {de} }