@article{deVeraAlawiBackhausetal.2019, author = {de Vera, Jean-Pierre Paul and Alawi, Mashal and Backhaus, Theresa and Baqu{\´e}, Mickael and Billi, Daniela and B{\"o}ttger, Ute and Berger, Thomas and Bohmeier, Maria and Cockell, Charles and Demets, Ren{\´e} and de la Torre Noetzel, Rosa and Edwards, Howell and Elsaesser, Andreas and Fagliarone, Claudia and Fiedler, Annelie and Foing, Bernard and Foucher, Fr{\´e}d{\´e}ric and Fritz, J{\"o}rg and Hanke, Franziska and Herzog, Thomas H. and Horneck, Gerda and H{\"u}bers, Heinz-Wilhelm and Huwe, Bj{\"o}rn and Joshi, Jasmin and Kozyrovska, Natalia and Kruchten, Martha and Lasch, Peter and Lee, Natuschka and Leuko, Stefan and Leya, Thomas and Lorek, Andreas and Mart{\´i}nez-Fr{\´i}as, Jes{\´u}s and Meessen, Joachim and Moritz, Sophie and Moeller, Ralf and Olsson-Francis, Karen and Onofri, Silvano and Ott, Sieglinde and Pacelli, Claudia and Podolich, Olga and Rabbow, Elke and Reitz, G{\"u}nther and Rettberg, Petra and Reva, Oleg and Rothschild, Lynn and Garcia Sancho, Leo and Schulze-Makuch, Dirk and Selbmann, Laura and Serrano, Paloma and Szewzyk, Ulrich and Verseux, Cyprien and Wadsworth, Jennifer and Wagner, Dirk and Westall, Frances and Wolter, David and Zucconi, Laura}, title = {Limits of Life and the Habitability of Mars: The ESA Space Experiment BIOMEX on the ISS}, series = {Astrobiology}, volume = {19}, journal = {Astrobiology}, number = {2}, issn = {1557-8070}, doi = {10.1089/ast.2018.1897}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-12048}, year = {2019}, abstract = {BIOMEX (BIOlogy and Mars EXperiment) is an ESA/Roscosmos space exposure experiment housed within the exposure facility EXPOSE-R2 outside the Zvezda module on the International Space Station (ISS). The design of the multiuser facility supports—among others—the BIOMEX investigations into the stability and level of degradation of space-exposed biosignatures such as pigments, secondary metabolites, and cell surfaces in contact with a terrestrial and Mars analog mineral environment. In parallel, analysis on the viability of the investigated organisms has provided relevant data for evaluation of the habitability of Mars, for the limits of life, and for the likelihood of an interplanetary transfer of life (theory of lithopanspermia). In this project, lichens, archaea, bacteria, cyanobacteria, snow/permafrost algae, meristematic black fungi, and bryophytes from alpine and polar habitats were embedded, grown, and cultured on a mixture of martian and lunar regolith analogs or other terrestrial minerals. The organisms and regolith analogs and terrestrial mineral mixtures were then exposed to space and to simulated Mars-like conditions by way of the EXPOSE-R2 facility. In this special issue, we present the first set of data obtained in reference to our investigation into the habitability of Mars and limits of life. This project was initiated and implemented by the BIOMEX group, an international and interdisciplinary consortium of 30 institutes in 12 countries on 3 continents. Preflight tests for sample selection, results from ground-based simulation experiments, and the space experiments themselves are presented and include a complete overview of the scientific processes required for this space experiment and postflight analysis. The presented BIOMEX concept could be scaled up to future exposure experiments on the Moon and will serve as a pretest in low Earth orbit.}, language = {en} } @article{BalabanovWilhelmVenzetal.2013, author = {Balabanov, Stefan and Wilhelm, Thomas and Venz, Simone and Keller, Gunhild and Scharf, Christian and Pospisil, Heike and Braig, Melanie and Barett, Christine and Bokemeyer, Carsten and Walther, Reinhard and Br{\"u}mmendorf, Tim H. and Schuppert, Andreas}, title = {Combination of a Proteomics Approach and Reengineering of Meso Scale Network Models for Prediction of Mode-of-Action for Tyrosine Kinase Inhibitors}, series = {PLoS ONE}, volume = {8}, journal = {PLoS ONE}, number = {1}, issn = {1932-6203}, doi = {10.1371/journal.pone.0053668}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-6370}, pages = {14}, year = {2013}, abstract = {In drug discovery, the characterisation of the precise modes of action (MoA) and of unwanted off-target effects of novel molecularly targeted compounds is of highest relevance. Recent approaches for identification of MoA have employed various techniques for modeling of well defined signaling pathways including structural information, changes in phenotypic behavior of cells and gene expression patterns after drug treatment. However, efficient approaches focusing on proteome wide data for the identification of MoA including interference with mutations are underrepresented. As mutations are key drivers of drug resistance in molecularly targeted tumor therapies, efficient analysis and modeling of downstream effects of mutations on drug MoA is a key to efficient development of improved targeted anti-cancer drugs. Here we present a combination of a global proteome analysis, reengineering of network models and integration of apoptosis data used to infer the mode-of-action of various tyrosine kinase inhibitors (TKIs) in chronic myeloid leukemia (CML) cell lines expressing wild type as well as TKI resistance conferring mutants of BCR-ABL. The inferred network models provide a tool to predict the main MoA of drugs as well as to grouping of drugs with known similar kinase inhibitory activity patterns in comparison to drugs with an additional MoA. We believe that our direct network reconstruction approach, demonstrated on proteomics data, can provide a complementary method to the established network reconstruction approaches for the preclinical modeling of the MoA of various types of targeted drugs in cancer treatment. Hence it may contribute to the more precise prediction of clinically relevant on- and off-target effects of TKIs.}, language = {en} } @article{SmekhovaSzyjkaLaTorreetal.2024, author = {Smekhova, Alevtina and Szyjka, Thomas and La Torre, Enrico and Ollefs, Katharina and Eggert, Benedikt and Coester, Birte and Wilhelm, Fabrice and Bali, Rantej and Lindner, J{\"u}rgen and Rogalev, A. and T{\"o}bbens, Daniel Maria and Weschke, Eugen and Luo, Chen and Chen, Kai and Radu, Florin and Schmitz-Antoniak, Carolin and Wende, Heiko}, title = {Irradiation-induced enhancement of Fe and Al magnetic polarizations in Fe60Al40 films}, series = {New Journal of Physics}, volume = {26}, journal = {New Journal of Physics}, publisher = {Institute of Physics Publishing (IOP)}, issn = {1367-2630}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18381}, year = {2024}, abstract = {The rise of Fe magnetic moment, changes in Al electronic structure and a variation of Al magnetic polarization in thin films of transition metal aluminide Fe60Al40 have been probed through the order-disorder phase transition by soft X-ray absorption spectroscopy and X-ray resonant magnetic reflectivity in the extreme ultraviolet regime. In a course of the transition induced by 20 keV Ne+ irradiation with low fluences (1014 ions·cm-2), X-ray magnetic circular dichroism spectra taken at the Fe L2,3 absorption edges at room and low temperatures revealed a pronounced increase of Fe 3d states spin-polarization. X-ray resonant magnetic reflectivity applied to the Al L2,3 and Fe M2,3 edges allowed to detect the magnetic polarization of Al atoms in the films. The changes in Al electronic structure have been seen by alteration of Al K edge X-ray absorption near edge structure. A difference in anisotropy fields for films before and after irradiation has been observed by element-specific hysteresis loops recorded at low temperatures in absorption and reflection geometries at the Fe L2,3 and M2,3 edges, respectively. An attempt to reduce the top oxide layer by an inductively coupled hydrogen plasma has shown a possibility to recover the chemically ordered phase.}, language = {en} } @article{MarehnWilhelmPospisiletal.2019, author = {Marehn, David Thomas and Wilhelm, Detlef and Pospisil, Heike and Pizzoferrato, Roberto}, title = {Double entry method for the verification of data a chromatography data system receives}, series = {Journal of Sensors and Sensor Systems}, volume = {8}, journal = {Journal of Sensors and Sensor Systems}, issn = {2194-878X}, doi = {10.5194/jsss-8-207-2019}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-12349}, pages = {207 -- 214}, year = {2019}, abstract = {The importance of software validation increases since the need for high usability and suitability of software applications grows. In order to reduce costs and manage risk factors, more and more recommendations and rules have been established. In the field of pharmacy the vendors of so-called chromatography data systems (CDSs) had to implement the guidelines of the Code of Federal Regulations Title 21 (CFR 21) during the last few years in order to fulfill the increasing requirements. The CFR 21 part 11 deals with electronic records and signatures. This part is binding for each company in the regulated environment that wishes to create, edit and sign electronic information instead of printing them on paper. Subsection CFR 21 part 11.10(h) explains how to perform an input check for manual user entries as well as for data that will be collected from an external device. In this article we present an approach performing the double entry method on data provided by the hardware instrument in order to investigate possible influences on the raw data by the handling CDS. A software tool has been written which allows us to communicate with a high-performance liquid chromatography (HPLC) detector and acquire data from it. The communication is completely independent of a CDS which is started separately and connected to the same system. Using this configuration we made a parallel data acquisition of two instances at the same time possible. Two CDSs have been tested and for at least one of them it has been shown that a comparison of the acquired data can be done as with the double entry method for the data verification. For the second CDS we checked whether it would be applicable after a few modifications. The given approach could be either used for a live data verification of produced raw data or as a single test during a software operational qualification to verify the data acquisition functionality of the software.}, language = {en} }