@article{KnihaDvořakHaladaetal.2020, author = {Kniha, Edwin and Dvoř{\´a}k, V{\´i}t and Halada, Petr and Milchram, Markus and Obwaller, Adelheid G. and Kuhls, Katrin and Schlegel, Susanne and K{\"o}hsler, Martina and Poeppl, Wolfgang and Bakran-Lebl, Karin and Fuehrer, Hans-Peter and Volfov{\´a}, Věra and Mooseder, Gerhard and Ivovic, Vladimir and Volf, Petr and Walochnik, Julia}, title = {Integrative Approach to Phlebotomus mascittii Grassi, 1908: First Record in Vienna with New Morphological and Molecular Insights}, series = {Pathogens}, volume = {9}, journal = {Pathogens}, number = {12}, publisher = {MDPI}, issn = {2076-0817}, doi = {10.3390/pathogens9121032}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-13600}, year = {2020}, abstract = {Sand flies (Diptera: Psychodidae: Phlebotominae) are blood-feeding insects that transmit the protozoan parasites Leishmania spp. and various arthropod-borne (arbo) viruses. While in Mediterranean parts of Europe the sand fly fauna is diverse, in Central European countries including Austria mainly Phlebotomus mascittii is found, an assumed but unproven vector of Leishmania infantum. To update the currently understudied sand fly distribution in Austria, a sand fly survey was performed and other entomological catches were screened for sand flies. Seven new trapping locations of Ph. mascittii are reported including the first record in Vienna, representing also one of the first findings of this species in a city. Morphological identification, supported by fluorescence microscopy, was confirmed by two molecular approaches, including sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) protein profiling. Sand fly occurrence and activity were evaluated based on surveyed locations, habitat requirements and climatic parameters. Moreover, a first comparison of European Ph. mascittii populations was made by two marker genes, cytochrome c oxidase subunit 1 (COI), and cytochrome b (cytb), as well as MALDI-TOF mass spectra. Our study provides new important records of Ph. mascittii in Austria and valuable data for prospective entomological surveys. MALDI-TOF MS protein profiling was shown to be a reliable tool for differentiation between sand fly species. Rising temperatures and globalization demand for regular entomological surveys to monitor changes in species distribution and composition. This is also important with respect to the possible vector competence of Ph. mascittii.}, language = {en} } @article{SchlegelKrumnowBoehmeetal.2021, author = {Schlegel, Susanne and Krumnow, Erik and B{\"o}hme, Andrea and Minieri, Marilena and Foitzik, Andreas}, title = {Miniaturized Blood Sampling System with Integrated Sample Preparation}, series = {Materials Science Forum}, volume = {1016}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18812}, pages = {1280 -- 1284}, year = {2021}, abstract = {Blood sampling as well as sample preparation are time consuming and requires a strict procedure, which is generally performed by medical trained personal. Not carrying out the procedure correctly could result in an infection of the patient or contamination of the sample itself. These limitations should be especially considered in case of pandemic outbreaks. In order to handle such a high number of patients a novel sample preparation system paired with modern blood sampling procedure is necessary. For this reason, a new device for blood sampling and preparation is designed containing an integrated microfluidic system. The fabrication is carried out by utilizing micro moulding of PDMS as well as micro milling. A first set of initial experiments as part of a first-generation study shows promising results. However, further steps of optimisation considering flow time and preparation cycle are part of a second-generation study.}, language = {en} }