@article{JanowskiGrohmeFrohmeetal.2014, author = {Janowski, Susann and Grohme, Markus and Frohme, Marcus and Wink, Michael}, title = {Development of New Microsatellite (STR) Markers for Montagu's Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing}, series = {The Open Ornithology Journal}, volume = {7}, journal = {The Open Ornithology Journal}, issn = {1874-4532}, doi = {10.2174/1874453201407010011}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-5966}, pages = {11 -- 18}, year = {2014}, abstract = {During the last decades the ground-breeding Montagu's harrier ( Circus pygargus , Linnaeus, 1758) has changed its breeding habitats in Europe to agricultural areas in which many local populations would be close to extinction without a special nest protection regime. Although Montagu's harrier is a well-studied species in terms of ecology and breeding biology, its genetic structure and population genetics are almost unknown. As there is a lack of good genetic markers we developed a set of 19 microsatellite markers comprising 16 new STR markers which were identified by next-generation sequencing (NGS) using 454 shot-gun pyrosequencing of genomic DNA. The STR markers were arranged into three multiplex PCR sets for high throughput genotyping and characterised. The marker set provides a powerful tool for kinship analysis. The combined non-exclusion probability for parent pairs was 1.13* 10-11. Only three loci showed PIC values < 0.50. In total, 121 known family relationships were compared with genetically calculated ones to test the markers suitability for parentage analysis. In 97.5\% of all cases full-sibships were accurately determined and 97.6\% of all mothers were assigned correctly to their chicks. The present multiplex PCR panels can be used to investigate several hypotheses concerning breeding behaviour, kinship, exchange rates between populations and phylogeography.}, language = {en} }