@inproceedings{HollmannRegiererD'Eliaetal.2018, author = {Hollmann, Susanne and Regierer, Babette and D'Elia, Domenica and Frohme, Marcus and Gruden, Kristina and Pfeil, Juliane and Baebler, Spela and Sezerman, Ugur and Evelo, Chris T. and Erhart, Friederike and Huppertz, Berthold and Bongcam-Rudloff, Erik and Trefois, Christophe and Gruca, Aleksandra and Duca, Deborah and Colotti, Gianni and Merino-Martinez, Roxana and Ouzounis, Christos and Hunewald, Oliver and He, Feng and Kremer, Andreas}, title = {Standardisation in life-science research - Making the case for harmonization to improve communication and sharing of data amongst researchers}, editor = {Kalajdziski, Slobodan and Ackovska, Nevena}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-20118}, pages = {11}, year = {2018}, abstract = {Modern, high-throughput methods for the analysis of genetic information, gene and metabolic products and their interactions offer new opportunities to gain comprehensive information on life processes. The data and knowledge generated open diverse application possibilities with enormous innovation potential. To unlock that potential skills in generating but also properly annotating the data for further data integration and analysis are needed. The data need to be made computer readable and interoperable to allow integration with existing knowledge leading to actionable biological insights. To achieve this, we need common standards and standard operating procedures as well as workflows that allow the combination of data across standards. Currently, there is a lack of experts who understand the principles and possess knowledge of the principles and relevant tools. This is a major barrier hindering the implementation of FAIR (findable, accessible, interoperable and reusable) data principles and the actual reusability of data. This is mainly due to insufficient and unequal education of the scientists and other stakeholders involved in producing and handling big data in life science that is inherently varied and complex in nature, and large in volume. Due to the interdisciplinary nature of life science research, education within this field faces numerous hurdles including institutional barriers, lack of local availability of all required expertise, as well as lack of appropriate teaching material and appropriate adaptation of curricula.}, language = {en} } @article{SchlegelEngelsBifarettietal.2018, author = {Schlegel, Volker and Engels, Andreas and Bifaretti, Stefano and Foitzik, Andreas}, title = {Validation of Material Parameters of Collagen Compositions for the Application as a Bioink}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18764}, pages = {2437 -- 2441}, year = {2018}, abstract = {The novel approach of an individualized medicine affects nowadays various areas of therapeutic treatment. Primarily, these include the application of pharmaceuticals, private point-of-care solutions, surgical procedures as well as steps of rehabilitation. However, beside these existing strategies the development of patient specific models for the training of clinical personal is currently insufficient. Such models are essential to prepare a personalized approach of medical care. One possible solution to address this problem could be the adaption of the established 3d printing technology for the processing of suitable biopolymers. The presented work is focused on the development of a printing system utilizing collagen. Therefore, it is intended to design multiple extrusion heads in accordance with the material parameters. In order to achieve the aspired physiological properties of the final model, a first set of experiments will be performed with several compositions of collagen to validate the fundamental mechanical characteristics. Hereby, the elasticity, thermal stability, force resistance as well as the haptic behaviour are of most interest. Afterwards, these obtained experimental results should be used to simulate the extrusion process and to validate the extruder concepts. In case of a positive evaluation these concepts are realized by using rapid prototyping technologies. Finally, this novel 3d printer will be used to print first organic test structures with collagen.}, language = {en} } @article{LobsteinZinnMinierietal.2018, author = {Lobstein, Susanne and Zinn, Steffen and Minieri, Marilena and Foitzik, Andreas}, title = {Sample Analysis for Novel Medical Applications}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18749}, pages = {2518 -- 2521}, year = {2018}, abstract = {The sample preparation for biological and chemical probes involves following a strict workflow to eliminate any contamination to the sample beforehand. Furthermore, it is time consuming and must be carried out by trained personnel such as a nurse or other supervisors, making it therefore expensive. The development of novel sample preparation techniques paired with modern sample analysis systems is focused on improving the operability while keeping a constant quality of results. This is important to analyse samples, which cannot be determined with current screening conditions. The analysis of analytes is required to receive a more detailed picture of the patient and to fully understand its complexity. Possible samples for in-depth analysis of chemical origin can be cholesterol or glucose. More complex samples, such as blood or saliva, require a sophisticated system, which analyses the samples for their individual compounds.}, language = {en} } @article{MatuschekLiebscherSantoetal.2018, author = {Matuschek, Nelson and Liebscher, Thilo and Santo, Loredana and Foitzik, Andreas}, title = {Online Glucose Measurement in DMEM for In Vitro Cell Culture}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18734}, pages = {2501 -- 2506}, year = {2018}, abstract = {In-vitro cell culture offers the ability to grow individual cells and monitor their behaviour in a controlled environment over a certain time. During culture, different parameters have to be controlled to ensure a vital growth of cells. The most important parameters are temperature, pH-value, oxygen and carbon dioxide levels as well as the glucose concentration. All of these parameters influence the growth ability of the cell culture and should be monitored online. The online monitoring of glucose was achieved with a novel GOD based sensor which enabled a real-time measurement of glucose during cell culture. A first-time characterisation of the sensor was carried out in the culture medium DMEM. In addition, material properties of the culture system were investigated. At first to establish a baseline measurement, the sensor was placed in different glucose concentrations dissolved in Water-KCl mixture at 25 °C inside a batch chamber under constant stirring. Afterwards, the temperature was increased to 37 °C to simulate a cell culture environment. In addition, the system was transferred into a flow-through reaction chamber. The highest response signal and the most stable signal was achieved at 37 °C with DMEM during flow-through measurement. Based on these measurements online glucose monitoring in cell culture was possible to determine the glucose consumption for each cell which is important for future human-on-a-chip devices to prevent diabetic metabolisms.}, language = {en} } @article{GottschalkKrumnowLietzauetal.2018, author = {Gottschalk, Josefine Johanna and Krumnow, Erik and Lietzau, Kai-Henning and Foitzik, Andreas and Richetta, Maria}, title = {Monitoring of Cytotoxic Induced Cellular Displacements by Utilizing Electronic Speckle Pattern Interferometry}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18728}, pages = {2513 -- 2517}, year = {2018}, abstract = {Modern medical science delivers through innovative chemical or mechanical/physical means new strategies to treat patients mildly and fight diseases accurately. In line with this development a screening procedure for tissue samples under usage of the electronic speckle pattern interferometry is developed at the University of Applied Science Wildau. The paper at hand provides the corner stone for such a procedure in form of an incubation system that is adapted to the properties of an electronic speckle pattern interferometer and allows the incubation as well as study of samples over time. As a result the developed system can regulate its own temperature and is constructed for use in an electronic speckle pattern interferometry (ESPI) setup. Its design allows a simple modular approach for further development.}, language = {en} } @article{EngelsZinnBonaiutoetal.2018, author = {Engels, Andreas and Zinn, Steffen and Bonaiuto, Vincenzo and Foitzik, Andreas}, title = {Improved Syringe Extruder for Improved Processing of Biological Materials}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18701}, pages = {2442 -- 2447}, year = {2018}, abstract = {3 dimensional (3D) printing evolved during the last decade to a consumer friendly and affordable craft. Furthermore, implementations of this techniques in the field of biotechnological research and development within laboratories is a very expansive process. Bio-printers' prices cover a wide spectrum and most basic models are available for around 5000€. On the other end, high-end printer machines with a vast variety of features are available for several hundreds of thousands of euros. Thus, due to the immense potential in the field of Biotechnology the availability of this technology for research purpose should be enhanced. A developed ecological syringe extruder prototype for processing of biological based gels has been further improved. The original prototype was capable to processing multiple layers of agar with concentrations of 1\% and 2.5\%. Based on these results the prototype was revised regarding printing process parameter, which include among others applied forces to the substrate, air-ventilation, and heating of the substrate. The process behavior will be simulated with computational fluid dynamics for the processing of biological based substrate. After a concluding validation these results are intended to be implemented into a new design for improved processing of a variety of bioinks.}, language = {en} } @article{BoehmeSchuetzeSaueretal.2018, author = {B{\"o}hme, Andrea and Sch{\"u}tze, Felix and Sauer, Sabine and Foitzik, Andreas}, title = {Fabrication and Validation by Micromilling for Bioreactor Prototyping}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18699}, pages = {2448 -- 2453}, year = {2018}, abstract = {Bioreactor systems for cultivating cells in Life Sciences have been widely used for decades. Recently, there is a trend towards miniaturization, disposables and even micro platforms that fulfill increasing demands strongly aiming for production and testing of novel pharmaceutical products. Miniaturized bioreactors allow low power consumption, portability and reduced space requirements and utilize smaller volumes of reagents and samples [1,2]. A recursive strategy is necessary for optimizing the design and the manufacture of such miniaturized bioreactors. For the fabrication of these prototypes utilized micro-milling. Micro milling is a mechanical process which is commonly applied to create micro-structures in metals, e.g. aluminum and steel, or polymers, e.g. poly carbonate substrates. The structures and geometries are generated by utilizing computer aided design. By means of computer-aided manufacturing, the machining operations are implemented and then transferred to the machine tool. The machine tool moves the cutting tools with certain speeds, feeds and traverse ranges to the substrate. Micro milling has the advantage that the materials are generally not degraded by chemical substances, heating procedures or electromagnetic radiation.}, language = {en} } @article{SchneiderSeligKeiletal.2018, author = {Schneider, Sylvio and Selig, Martina and Keil, Verena and Lehmann, Matthias and Foitzik, Andreas and Casalboni, Mauro and Richetta, Maria}, title = {Development of a Smartphone Based Reader for the Quantitative Analysis of Lateral Flow Assays}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18688}, pages = {2522 -- 2527}, year = {2018}, abstract = {Smartphones are developing into all-purposes devices. In the present work, the employment/application of smartphones as medical devices in home care and point-of-care (POC) diagnostics are investigated in the analysis of Lateral Flow Assays (LFA). A smartphone-based LFA reader was developed for the quantitative analysis of D-Dimer - a biomarker indicating e.g. thrombotic event or danger of embolism.The proof-of-concept has been shown with multiple smartphones in establishing: (I) Optimal dimensions of the LFA cell of 72.11mm distance of smartphone to D-Dimer test leading to a coefficients of variances (CV) between 0.8\% and 4.2\%. (II) Inter-device investigations: CVs around 13.5\%; a limit of detection (LOD) of 100ng/ml (DDU) D-Dimer. (III) Inter-smartphone investigations: CV about 16\%, a limit of detection (LOD) at 66.4ng/ml (DDU). (IV) Calibrations: CV and LOD of three smartphones are comparable to the commercial available LFA reader. Further development to put the multiple smartphone-based LFA reader on the market.}, language = {en} } @article{GloesBoehmeLiebscheretal.2018, author = {Gl{\"o}s, Franziska and B{\"o}hme, Andrea and Liebscher, Thilo and Zinn, Steffen and Richetta, Maria and Foitzik, Andreas}, title = {Analyte Tracking for Novel Bio-Applications}, series = {Materials Science Forum}, volume = {941}, journal = {Materials Science Forum}, publisher = {Trans Tech Publications}, issn = {0255-5476}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-18676}, pages = {2454 -- 2457}, year = {2018}, abstract = {Modern cell culture as well as sophisticated bio-applications involve complex biochemical processes, which are required to induce growth, product development or material degradation. Tracking the reaction processes inside the application presents a major challenge due to its complexity. The development of new analysis and tracking mechanisms for such application presents a solution to fully understand the process. In addition, the applied sensors are required to monitor the reactions enable a live tracking of the process. Furthermore, this gives the opportunity to influence and manipulate reactions to further enhance the application of the process. Possible analytes for tracking during processes can be chemical origin such as glucose, cytokines, antibiotics and growth factors, which are included in the culture medium. Based on the complexity of the culture or bio-application the sensor tracking mechanism has to be adapted to ensure full process control. A variety of different approaches can be used for the tracking mechanism.}, language = {en} } @inproceedings{GilaniVillringerZhangetal.2018, author = {Gilani, Taravat Saeb and Villringer, Claus and Zhang, Edward and Gundlach, H. and Buchmann, Jens and Schrader, Sigurd and Laufer, Jan}, title = {Parallelised photoacoustic signal acquisition using a Fabry-Perot sensor and a camera-based interrogation scheme}, publisher = {SPIE}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-15335}, pages = {217 -- 222}, year = {2018}, abstract = {Tomographic photoacoustic (PA) images acquired using a Fabry-Perot (FP) based scanner offer high resolution and image fidelity but can result in long acquisition times due to the need for raster scanning. To reduce the acquisition times, a parallelised camera-based PA signal detection scheme is developed. The scheme is based on using a sCMOScamera and FPI sensors with high homogeneity of optical thickness. PA signals were acquired using the camera-based setup and the signal to noise ratio (SNR) was measured. A comparison of the SNR of PA signal detected using 1) a photodiode in a conventional raster scanning detection scheme and 2) a sCMOS camera in parallelised detection scheme is made. The results show that the parallelised interrogation scheme has the potential to provide high speed PA imaging.}, language = {en} } @article{KoelschHejaziStiegeretal.2018, author = {K{\"o}lsch, Adrian and Hejazi, Mahdi and Stieger, Kai Ralf and Feifel, Sven Christian and Kern, Jan F. and M{\"u}h, Frank and Lisdat, Fred and Lokstein, Heiko and Zouni, Athina}, title = {Insights into the binding behavior of native and non-native cytochromes to photosystem I from Thermosynechococcus elongatus}, series = {Journal of Biological Chemistry}, volume = {293}, journal = {Journal of Biological Chemistry}, number = {23}, issn = {1083-351X}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-12780}, pages = {9090 -- 9100}, year = {2018}, abstract = {The binding of photosystem I (PS I) from Thermosynechococcus elongatus to the native cytochrome (cyt) c6 and cyt c from horse heart (cyt cHH) was analyzed by oxygen consumption measurements, isothermal titration calorimetry (ITC), and rigid body docking combined with electrostatic computations of binding energies. Although PS I has a higher affinity for cyt cHH than for cyt c6, the influence of ionic strength and pH on binding is different in the two cases. ITC and theoretical computations revealed the existence of unspecific binding sites for cyt cHH besides one specific binding site close to P700. Binding to PS I was found to be the same for reduced and oxidized cyt cHH. Based on this information, suitable conditions for cocrystallization of cyt cHH with PS I were found, resulting in crystals with a PS I:cyt cHH ratio of 1:1. A crystal structure at 3.4-{\AA} resolution was obtained, but cyt cHH cannot be identified in the electron density map because of unspecific binding sites and/or high flexibility at the specific binding site. Modeling the binding of cyt c6 to PS I revealed a specific binding site where the distance and orientation of cyt c6 relative to P700 are comparable with cyt c2 from purple bacteria relative to P870. This work provides new insights into the binding modes of different cytochromes to PS I, thus facilitating steps toward solving the PS I-cyt c costructure and a more detailed understanding of natural electron transport processes.}, language = {en} } @article{vandeSandSchulzRitzmannetal.2018, author = {van de Sand, Ron and Schulz, Sebastian and Ritzmann, Kay and Reiff-Stephan, J{\"o}rg}, title = {Vernetzung physischer und virtueller Entit{\"a}ten im CPPS : Augmented Reality als Mensch-Maschine Schnittstelle}, series = {atp magazin}, volume = {9}, journal = {atp magazin}, issn = {2190-4111}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-12739}, pages = {36 -- 43}, year = {2018}, abstract = {Konventionelle Fertigungsstrategien werden k{\"u}nftig zunehmend durch komplexe cyberphysische Produktionssysteme (CPPS) ersetzt. Es entstehen durchgehend verbundene Wertsch{\"o}pfungsnetzwerke mit großer Informationsdichte, die den Menschen mit einer signifikant gestiegenen Datenmenge konfrontieren, auf deren Basis er agieren muss. Daher nimmt die Mensch-Maschine-Schnittstelle einen wichtigen Stellenwert innerhalb des Produktionsprozesses ein, wobei sich neuartige Visualisierungsverfahren wie Augmented Reality (AR) vielversprechend zeigen. F{\"u}r entsprechende Anwendungen bedarf es einer durchg{\"a}ngigen Kommunikation aus dem Feld bis hin zur Applikation. Der Artikel beschreibt, wie existierende Kommunikationsstandards daf{\"u}r nutzbar gemacht werden k{\"o}nnen und zeigt eine beispielhafte Umsetzung. Zudem wird eine Kommunikationsarchitektur vorgeschlagen, die auf Grundlage der Verwaltungsschale den interoperablen Informationsaustausch zwischen physischen und virtuellen Entit{\"a}ten erm{\"o}glicht.}, language = {de} } @article{SteglichMaiVillringeretal.2018, author = {Steglich, Patrick and Mai, Christian and Villringer, Claus and Pulwer, Silvio and Casalboni, Mauro and Schrader, Sigurd and Mai, Andreas}, title = {Quadratic electro-optic effect in silicon-organic hybrid slot-waveguides}, series = {Optics Letters}, volume = {43}, journal = {Optics Letters}, number = {15}, issn = {1539-4794}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-12148}, year = {2018}, abstract = {This Letter reports on the quadratic electro-optic effect of polymers, observed in a silicon slot-waveguide at low voltages. We demonstrate that in narrow slots, the electro-optic response with respect to refractive index change is strong enough for on-chip wavelength tuning and intensity modulation using voltages as low as 1 V. A silicon slot-waveguide embedded by a nonlinear optical polymer, consisting of the dye Disperse Red 1 in poly(methyl methacrylate), serves as the phase shifter in a racetrack ring resonator. As deduced from the experimental data, the third-order susceptibility of the utilized electro-optic polymer is about 2·10-19 m2/V2. The demonstrated low-voltage operation and inherently thermal stability show the potential for silicon-organic hybrid devices using the quadratic electro-optic effect.}, language = {en} } @unpublished{vandeSandSchulzRitzmannetal.2018, author = {van de Sand, Ron and Schulz, Sebastian and Ritzmann, Kay and Reiff-Stephan, J{\"o}rg}, title = {Vernetzung von physischen und virtuellen Entit{\"a}ten im cyberphysischen Produktionssystem}, series = {atp magazin}, volume = {9}, journal = {atp magazin}, issn = {2190-4111}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10611}, year = {2018}, abstract = {Konventionelle Fertigungsstrategien werden k{\"u}nftig zunehmend durch komplexe cyberphysische Produktionssysteme (CPPS) ersetzt. Es entstehen durchgehend verbundene Wertsch{\"o}pfungsnetzwerke mit großer Informationsdichte, die den Menschen mit einer signifikant gestiegenen Datenmenge konfrontieren, auf deren Basis er agieren muss. Daher nimmt die Mensch-Maschine-Schnittstelle einen wichtigen Stellenwert innerhalb des Produktionsprozesses ein, wobei sich neuartige Visualisierungsverfahren wie Augmented Reality (AR) vielversprechend zeigen. F{\"u}r entsprechende Anwendungen bedarf es einer durchg{\"a}ngigen Kommunikation aus dem Feld bis hin zur Applikation. Der Artikel beschreibt, wie existierende Kommunikationsstandards daf{\"u}r nutzbar gemacht werden k{\"o}nnen und zeigt eine beispielhafte Umsetzung. Zudem wird eine Kommunikationsarchitektur vorgeschlagen, die auf Grundlage der Verwaltungsschale den interoperablen Informationsaustausch zwischen physischen und virtuellen Entit{\"a}ten erm{\"o}glicht.}, language = {de} } @article{GrebinykGrebinykPrylutskaetal.2018, author = {Grebinyk, Anna and Grebinyk, Sergii and Prylutska, Svitlana and Ritter, Uwe and Matyshevska, Olga and Dandekar, Thomas and Frohme, Marcus}, title = {C60 fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy}, series = {Free Radical Biology and Medicine}, volume = {124}, journal = {Free Radical Biology and Medicine}, issn = {1873-4596}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10588}, pages = {319 -- 327}, year = {2018}, abstract = {Recent progress in nanobiotechnology has attracted interest to a biomedical application of the carbon nanostructure C60 fullerene since it possesses a unique structure and versatile biological activity. C60 fullerene potential application in the frame of cancer photodynamic therapy (PDT) relies on rapid development of new light sources as well as on better understanding of the fullerene interaction with cells. The aim of this study was to analyze C60 fullerene effects on human leukemic cells (CCRF-CEM) in combination with high power single chip light-emitting diodes (LEDs) light irradiation of different wavelengths: ultraviolet (UV, 365 nm), violet (405 nm), green (515 nm) and red (632 nm). The time-dependent accumulation of fullerene C60 in CCRF-CEM cells up to 250 ng/106 cells at 24 h with predominant localization within mitochondria was demonstrated with immunocytochemical staining and liquid chromatography mass spectrometry. In a cell viability assay we studied photoexcitation of the accumulated C60 nanostructures with ultraviolet or violet LEDs and could prove that significant phototoxic effects did arise. A less pronounced C60 fullerene phototoxic effect was observed after irradiation with green, and no effect was detected with red light. A C60 fullerene photoactivation with violet light induced substantial ROS generation and apoptotic cell death, confirmed by caspase3/7 activation and plasma membrane phosphatidylserine externalization. Our work proved C60 fullerene ability to induce apoptosis of leukemic cells after photoexcitation with high power single chip 405 nm LED as a light source. This underlined the potential for application of C60 nanostructure as a photosensitizer for anticancer therapy.}, language = {en} } @incollection{Steglich2018, author = {Steglich, Patrick}, title = {Silicon-on-Insulator Slot Waveguides: Theory and Applications in Electro-Optics and Optical Sensing}, series = {Emerging Waveguide Technology}, booktitle = {Emerging Waveguide Technology}, editor = {You, Kok Yeow}, publisher = {IntechOpen}, isbn = {978-1-78923-493-0}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10563}, publisher = {Technische Hochschule Wildau}, pages = {187 -- 210}, year = {2018}, abstract = {This chapter deals with the basic concept of silicon-on-insulator (SOI) slot waveguides,including slot waveguide theory, fabrication steps, and applications. First, in the theorysection, a modal field expression and the characteristic equation is derived, which is alsovalid for higher-order modes. SOI slot waveguide structures are simulated and character-istic values like the effective refractive indices and the field confinement factors are deter-mined. The fabrication section describes typical SOI fabrication steps and the limits ofcurrent fabrication techniques. Additionally, developments regarding loss reduction inSOI slot waveguides are given from the fabrication point of view. This is followed by thetheory and practice of slot waveguide based electro-optical modulators. Here, the SOI slotwaveguide is embedded in an organic nonlinear optical material in order to achieverecord-low voltage-length products. In the field of optical sensors, it is shown that slotwaveguides enable remarkable waveguide sensitivity for both refractive index sensingand surface sensing.}, language = {en} } @article{GerhardStappenbeck2018, author = {Gerhard, Christoph and Stappenbeck, Marco}, title = {Impact of the Polishing Suspension Concentration on Laser Damage of Classically Manufactured and Plasma Post-Processed Zinc Crown Glass Surfaces}, series = {Applied Sciences}, volume = {8}, journal = {Applied Sciences}, number = {9}, publisher = {MDPI}, issn = {2076-3417}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10556}, pages = {1 -- 15}, year = {2018}, abstract = {The laser-induced damage threshold of optics is an issue of essential importance in high-power laser applications. However, the complex and partially interacting mechanisms as well as the underlying reasons for laser damage of glass surfaces are not yet fully understood. The aim of the present work is to contribute to a better understanding of such damage mechanisms by providing original results on the impact of classical glass surface machining on the laser-induced damage threshold. For this purpose, glass samples were prepared with well-defined process conditions in terms of the used lapping and polishing agents and suspensions. Further, the samples were post-processed by atmospheric pressure plasma for precision cleaning. The laser-induced damage threshold and surface contamination by residues from the manufacturing process were determined before and after plasma post-processing. It is shown that the polishing suspension concentration has a certain impact on the laser-induced damage threshold and surface contamination by residues from used working materials. The highest damage threshold of 15.2 J/cm² is found for the lowest surface contamination by carbon which occurs in the case of the highest polishing suspension concentration. After plasma treatment for merely 60 s, this value was increased to 20.3 J/cm² due to the removal of surface-adherent carbon. The results thus imply that the laser-induced damage threshold can notably be increased by first choosing appropriate process parameters during classical manufacturing and second plasma post-processing for surface finishing.}, language = {en} } @incollection{BorndoerferKarbsteinLiebchenetal.2018, author = {Bornd{\"o}rfer, Ralf and Karbstein, Marika and Liebchen, Christian and Lindner, Niels}, title = {A Simple Way to Compute the Number of Vehicles That Are Required to Operate a Periodic Timetable}, series = {18th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2018)}, booktitle = {18th Workshop on Algorithmic Approaches for Transportation Modelling, Optimization, and Systems (ATMOS 2018)}, editor = {Bornd{\"o}rfer, Ralf and Storandt, Sabine}, publisher = {Schloss Dagstuhl - Leibniz-Zentrum f{\"u}r Informatik GmbH}, address = {Wadern}, isbn = {978-3-95977-096-5}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10549}, publisher = {Technische Hochschule Wildau}, pages = {16:1 -- 16:15}, year = {2018}, abstract = {We consider the following planning problem in public transportation: Given a periodic timetable, how many vehicles are required to operate it? In [Julius Paetzold et al., 2017], for this sequential approach, it is proposed to first expand the periodic timetable over time, and then answer the above question by solving a flow-based aperiodic optimization problem. In this contribution we propose to keep the compact periodic representation of the timetable and simply solve a particular perfect matching problem. For practical networks, it is very much likely that the matching problem decomposes into several connected components. Our key observation is that there is no need to change any turnaround decision for the vehicles of a line during the day, as long as the timetable stays exactly the same.}, language = {en} } @article{DevulapallyBuergerMielkeetal.2018, author = {Devulapally, Praneeth Reddy and B{\"u}rger, J{\"o}rg and Mielke, Thorsten and Konthur, Zolt{\´a}n and Lehrach, Hans and Yaspo, Marie-Laure and Gl{\"o}kler, J{\"o}rn and Warnatz, Hans-J{\"o}rg}, title = {Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes}, series = {Genome Medicine}, volume = {10}, journal = {Genome Medicine}, issn = {1756-994X}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10538}, pages = {1 -- 12}, year = {2018}, abstract = {Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.}, language = {en} } @article{GerhardGimpelTascheetal.2018, author = {Gerhard, Christoph and Gimpel, Thomas and Tasche, Daniel and Koch, Jennifer and Br{\"u}ckner, Stephan and Flachenecker, G{\"u}nter and Wieneke, Stephan and Schade, Wolfgang and Vi{\"o}l, Wolfgang}, title = {Atmospheric pressure plasma-assisted femtosecond laser engraving of aluminium}, series = {Journal of Physics D: Applied Physics}, volume = {51}, journal = {Journal of Physics D: Applied Physics}, issn = {1361-6463}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10521}, pages = {1 -- 7}, year = {2018}, abstract = {In this contribution, we report on the impact of direct dielectric barrier discharge argon plasma at atmospheric pressure on femtosecond laser engraving of aluminium. It is shown that the assisting plasma strongly affects the surface geometry and formation of spikes of both laser-engraved single lines and patterns of adjacent lines with an appropriate overlap. Further, it was observed that the overall ablation depth is significantly increased in case of large-scale patterning whereas no notable differences in ablation depth are found for single lines. Several possible mechanisms and underlying effects of this behaviour are suggested. The increase in ablation depth is supposed to be due to a plasma-induced removal of debris particles from the cutting point via charging and oxidation as supported by EDX analysis of the re-solidified debris. Furthermore, the impact of a higher degree of surface wrinkling as well as direct interactions of plasma species with the aluminium surface on the ablation process are discussed.}, language = {en} } @article{GrebinykPrylutskaGrynyuketal.2018, author = {Grebinyk, Anna and Prylutska, Svitlana and Grynyuk, I. and Kolp, Benjamin and Hurmach, V. and Sliva, T. and Amirkhanov, Volodymyr and Trush, V. and Matyshevska, Olga and Slobodyanik, M. and Prylutskyy, Yuriy and Frohme, Marcus and Ritter, Uwe}, title = {C60 Fullerene Effects on Diphenyl-N-(trichloroacetyl)-amidophosphate Interaction with DNA In Silico and Its Cytotoxic Activity Against Human Leukemic Cell Line In Vitro}, series = {Nanoscale Research Letters}, volume = {2018}, journal = {Nanoscale Research Letters}, issn = {1556-276X}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10515}, pages = {1 -- 9}, year = {2018}, abstract = {New representative of carbacylamidophosphates - diphenyl-N-(trichloroacetyl)-amidophosphate (HL), which contains two phenoxy substituents near the phosphoryl group, was synthesized, identified by elemental analysis and IR and NMR spectroscopy, and tested as a cytotoxic agent itself and in combination with C60 fullerene. According to molecular simulation results, C60 fullerene and HL could interact with DNA and form a rigid complex stabilized by stacking interactions of HL phenyl groups with C60 fullerene and DNA G nucleotide, as well as by interactions of HL CCl3 group by ion-π bonds with C60 molecule and by electrostatic bonds with DNA G nucleotide. With the use of MTT test, the cytotoxic activity of HL against human leukemic CCRF-CM cells with IC50 value detected at 10 μM concentration at 72 h of cells treatment was shown. Under combined action of 16 μM C60 fullerene and HL, the value of IC50 was detected at lower 5 μM HL concentration and at earlier 48 h period of incubation, besides the cytotoxic effect of HL was observed at a low 2.5 μM concentration at which HL by itself had no influence on cell viability. Binding of C60 fullerene and HL with minor DNA groove with formation of a stable complex is assumed to be one of the possible reasons of their synergistic inhibition of CCRF-CЕM cells proliferation. Application of C60 fullerene in combination with 2.5 μM HL was shown to have no harmful effect on structural stability of blood erythrocytes membrane. Thus, combined action of C60 fullerene and HL in a low concentration potentiated HL cytotoxic effect against human leukemic cells and was not followed by hemolytic effect.}, language = {en} } @article{PfeilFrohmeSchulze2018, author = {Pfeil, Juliane and Frohme, Marcus and Schulze, Katja}, title = {Mobile microscopy for the examination of blood samples}, series = {EMBnet.journal}, volume = {23}, journal = {EMBnet.journal}, issn = {2226-6089}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10509}, pages = {e904}, year = {2018}, abstract = {The analysis of blood is one of the best possibilities to diagnose and control diseases and deficiency symptoms. Common blood tests that are performed in medical laboratories are time-consuming and work-intensive. In under-developed areas, there is often also a lack of specialised staff and facilities. The development of a mobile microscopic system that contains an automated image analysis and that can be used via a smartphone, could represent a valuable help to improve the diagnostic care, especially in those areas. it aims to enable a very fast, cheap, location- and knowledge-independent application for many use cases.}, language = {en} } @article{RoeschTolkiehn2018, author = {R{\"o}sch, Olga and Tolkiehn, G{\"u}nter-Ulrich}, title = {Zum Diskurs {\"u}ber die Sprache in der Wissenschaftskommunikation}, series = {Die neue Hochschule : DNH}, journal = {Die neue Hochschule : DNH}, number = {4}, issn = {0340-448X}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10465}, pages = {26 -- 29}, year = {2018}, abstract = {Der Diskurs {\"u}ber die Wissenschaftssprache darf nicht von außerwissenschaftlichen Interessen bestimmt werden. Er muss in den Bereich der Wissenschaft zur{\"u}ckgeholt und dort freiheitlich mit wissenschaftlicher Rationalit{\"a}t gef{\"u}hrt werden.}, language = {de} } @article{GrebinykGrebinykPrylutskaetal.2018, author = {Grebinyk, Anna and Grebinyk, Sergii and Prylutska, Svitlana and Ritter, Uwe and Matyshevska, Olga and Dandekar, Thomas and Frohme, Marcus}, title = {HPLC-ESI-MS method for C60 fullerene mitochondrial content quantification}, series = {Data in Brief}, volume = {19}, journal = {Data in Brief}, issn = {2352-3409}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10368}, pages = {2047 -- 2052}, year = {2018}, abstract = {The presented dataset describes the quantification of carbon nanoparticle C60 fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography-electro spray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C60 fullerene. Then, human leukemic cells were incubated with C60 fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C60 fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions. This data article refers to the research article "C60 Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy" by Grebinyk et al.}, language = {en} } @article{LuxEdlingSiemrothetal.2018, author = {Lux, Helge and Edling, Matthias and Siemroth, Peter and Schrader, Sigurd}, title = {Fast and Cost-Effective Synthesis of High-Quality Graphene on Copper Foils Using High-Current Arc Evaporation}, series = {Materials}, volume = {11}, journal = {Materials}, number = {5}, issn = {1996-1944}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10359}, pages = {1 -- 10}, year = {2018}, abstract = {In this paper, we present an innovative and ultra-fast process for the deposition of high-quality graphene on different metal foils and thin metal films. The graphene layer can be homogeneously deposited in only 30 s process time. Due to the weak adhesion to the substrate material, the monolayer graphene is easy to transfer using the established processes. For the production, we use magnetic filtered high-current arc evaporation (Φ-HCA) with a solid, graphitic carbon source. This ultra-fast growth process can pave the way towards a cost-effective graphene synthesis for the mass production e.g., in a roll-to-roll process, avoiding time consuming established processes.}, language = {en} } @misc{Mueller2018, type = {Master Thesis}, author = {M{\"u}ller, Philipp}, title = {Mensch-Maschine-Kommunikation basierend auf Natural Language Processing: Evaluierung von M{\"o}glichkeiten f{\"u}r den Einsatz eines humanoiden Roboters im Kontext einer Hochschulbibliothek}, doi = {10.15771/ma_2018_1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10349}, school = {Technische Hochschule Wildau}, pages = {172}, year = {2018}, abstract = {Thematisiert wird der Weg, von einer theoretischen Betrachtung computerlinguistischer Grundlagen der Sprachverarbeitung, bis hin zu einer prototypischen Umsetzung einer Software zur nat{\"u}rlich sprachlichen Dialogkommunikation zwischen Mensch und Roboter. Die Arbeit widmet sich dabei zun{\"a}chst der Bereitstellung theoretischer Grundlagen (Definition, Geschichte, Funktionsweise, Herausforderungen, Aktuelle Lage) von Mensch-Maschine-Kommunikation unter Zuhilfenahme des Natural Language Processings. Darauf basierend werden Anforderungen aufgestellt, die eine softwarem{\"a}ßige Implementierung eines solchen Systems beschreiben. Dies geschieht unter der Beachtung von Rahmenbedingungen durch den themenstellenden Betrieb dieser Arbeit und den Grenzen des Robotersystems. Es folgt eine ausgiebige Evaluierung verschiedener NLP-Systeme anhand des Abgleichs von Anforderungen und zus{\"a}tzlicher Kennziffer-Messungen (Precision, Recall, F1 Score). In diesem Zusammenhang entsteht auch ein Testprogramm, welches ein automatisiertes Evaluierungsverfahren bereitstellt. Die beiden besten NLP-Systeme werden anschließend in ihrer Verwendung detailliert beschrieben. Eine prototypische Implementierung stellt die Verwendung eines NLP-Systems, zur Kommunikation von Mensch und Roboter, in einem konkreten Anwendungsfall unter Beweis.}, language = {de} } @misc{HotesFischer2018, author = {Hotes, Andreas G. and Fischer, Benjamin}, title = {Untersuchung der kapazitiven Auswirkungen einer Variation im Personaleinsatz in der Handgep{\"a}ck-Nachkontrolle einer Flughafen-Sicherheitskontrollspur am Beispiel des BER-Layouts}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_7}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10271}, pages = {53 -- 60}, year = {2018}, abstract = {Dieser wissenschaftliche Beitrag stellt die Untersuchung einer modernen Sicherheitskontrollspur f{\"u}r Passagiere und Handgep{\"a}ck dar, wie sie unter anderem am Flughafen Berlin-Brandenburg Willy Brandt (BER) Anwendung finden wird. Nach einer Beschreibung der Verfahrensabl{\"a}ufe einer Sicherheitskontrolle entsteht unter Verwendung realer Passagierparameter und analysierter Prozesszeiten mit der Simulationssoftware CAST Terminal ein detailliertes Modell, mit dem die Passagierkapazit{\"a}t der Kontrollspur untersucht und ein eventueller Engpass lokalisiert werden kann. Durch zielgerichtete Ver{\"a}nderungen in der Personalbesetzung der Handgep{\"a}ck-Nachkontrolle sollen daraus folgende kapazitive Auswirkungen bei einer Betrachtung unterschiedlicher Szenarien unter Variation der Alarmrate der Handgep{\"a}ckpr{\"u}fanlage untersucht werden. Mit steigender Alarmrate verschiebt sich der Kapazit{\"a}tsengpass aus der Gep{\"a}ckaufnahme in die Handgep{\"a}ck-Nachkontrolle, was den Einsatz eines zweiten Nachkontrolleurs erforderlich macht.}, language = {de} } @misc{HendrixHerzog2018, author = {Hendrix, Michael and Herzog, Michael}, title = {Schutzverhalten von weichballistischem ultrahochmolekularem Polyethylen}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_6}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10261}, pages = {47 -- 51}, year = {2018}, abstract = {Diese Publikation befasst sich mit dem Langzeitverhalten der Schutzeigenschaften von weichballistischem ultra-hochmolekularem Polyethylen. Vorrangig soll die Frage gekl{\"a}rt werden, ob dieses Material qualit{\"a}tsmindernde Erscheinungen im Schutzverhalten aufweist, die mit seinem fortschreitenden Alter einhergehen. Um diese Frage zu kl{\"a}ren, wurden anhand zahlreicher Beschussversuche an polizeilichen Schutzwesten und Pr{\"u}fmustern statistisch verl{\"a}ssliche Daten gesammelt und anschließend unter verschiedenen Blickwinkeln ausgewertet. Ein wesentliches Ergebnis der Untersuchungen ist, dass sich die Schutzeigenschaften von weichballistischem ultra-hochmolekularem Polyethylen zumindest {\"u}ber einen Zeitraum von 30 Jahren nicht nennenswert {\"a}ndern.}, language = {de} } @misc{RaguschJergovićGieseetal.2018, author = {Ragusch, Denny and Jergović, Michael and Giese, Lutz B. and Rolle, Siegfried}, title = {Einsatz der Brennstoffzelle als Netzersatzanlage sicherheitsrelevanter Digitalfunkstationen im Feldtest}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_5}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10251}, pages = {39 -- 45}, year = {2018}, abstract = {Es wird anhand des F{\"o}rderprojekts „Wasserstoff-Netzersatzanlagen der Beh{\"o}rden und Organisationen mit Sicherheitsaufgaben (BOS) des Landes Brandenburg" des Zentraldienstes der Polizei des Landes Brandenburg die Praxistauglichkeit von Brennstoffzellen-Netzersatzanlagen untersucht. Marktreife und kommerziell verf{\"u}gbare Brennstoffzellen wurden in 115 sicherheitsrelevanten Digitalfunkstationen im Fl{\"a}chenland Brandenburg als Ersatz von wartungsintensiven und umweltproblematischen Dieselgeneratoren f{\"u}r die (Not-)Stromversorgung installiert. Diese Netzersatzanlagen sind f{\"u}r eine {\"U}berbr{\"u}ckungszeit von 72 h unter realen Bedingungen ausgelegt und wurden im Feldtest, parallel zum realen Betrieb, {\"u}ber einen l{\"a}ngeren Einsatzzeitraum (ca. 2 Jahre) auf ihre Zuverl{\"a}ssigkeit und Verf{\"u}gbarkeit hin untersucht. Die Anforderungen an und die Realisierung der Brennstoffzellen-Netzersatzanlagen sowie die Feldtest-Versuchsplanung und deren Ergebnisse werden beschrieben und diskutiert. Die Praxistauglichkeit wird mit einer hohen Verf{\"u}gbarkeit {\"u}ber 97 \% und einer Funktionszuverl{\"a}ssigkeit von mehr als 95 \% erfolgreich best{\"a}tigt. Die Entwicklung weiterer Komponenten wird empfohlen.}, language = {de} } @misc{SteglichKsianzouVillringeretal.2018, author = {Steglich, Patrick and Ksianzou, Viachaslau and Villringer, Claus and Pulwer, Silvio and Schrader, Sigurd}, title = {Theorie der Modenlinienspektroskopie zur optischen Charakterisierung von Polymerschichten}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_4}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10248}, pages = {31 -- 38}, year = {2018}, abstract = {Die Modenlinienspektroskopie kann zur hochpr{\"a}zisen Charakterisierung von Polymerschichten bez{\"u}glich des Brechungsindex verwendet werden. Das vorliegende Manuskript behandelt die Theorie der Modenlinienspektroskopie und beginnt dabei mit den theoretischen Grundlagen eines planaren Wellenleiters, die zum Verst{\"a}ndnis der Modenlinienspektroskopie notwendig sind. Die hier pr{\"a}sentierten expliziten Herleitungen k{\"o}nnen genutzt werden, um die Modenlinienspektroskopie so zu modifizieren, dass auch die Messung des Pockelseffektes m{\"o}glich ist. Eine Anwendungsm{\"o}glichkeit soll in der zuk{\"u}nftigen Bestimmung des Pockelskoeffizienten bestehen, der f{\"u}r die Weiterentwicklung von polymerbasierten elektrooptischen Modulatoren ben{\"o}tigt wird. Zudem werden der Aufbau und die experimentelle Herangehensweisen beschrieben und die Brechungsindexbestimmung wird exemplarisch am Beispiel einer Wirt-Gast-Polymerschicht demonstriert.}, language = {de} } @misc{GedatFechnerFiebelkornetal.2018, author = {Gedat, Egbert and Fechner, Pascal and Fiebelkorn, Richard and Vandenhouten, Jan and Vandenhouten, Ralf}, title = {Image recognition of multi-perspective data for intelligent analysis of gestures and actions}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_3}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10230}, pages = {25 -- 30}, year = {2018}, abstract = {The BERMUDA project started in January 2015 and was successfully completed after less than three years in August 2017. A technical set-up and an image processing and analysis software were developed to record and evaluate multi-perspective videos. Based on two cameras, positioned relatively far from one another with tilted axes, synchronized videos were recorded in the laboratory and in real life. The evaluation comprised the background elimination, the body part classification, the clustering, the assignment to persons and eventually the reconstruction of the skeletons. Based on the skeletons, machine learning techniques were developed to recognize the poses of the persons and next for the actions performed. It was, for example, possible to detect the action of a punch, which is relevant in security issues, with a precision of 51.3 \% and a recall of 60.6 \%.}, language = {en} } @misc{StiegerFeifelLoksteinetal.2018, author = {Stieger, Kai Ralf and Feifel, Sven Christian and Lokstein, Heiko and Hejazi, Mahdi and Zouni, Athina and Lisdat, Fred}, title = {Biohybride Architekturen f{\"u}r eine effiziente Umwandlung von Licht in elektrische Energie durch Integration von Photosystem I in skalierbare mesopor{\"o}se 3D Elektroden}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_2}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10220}, pages = {17 -- 24}, year = {2018}, abstract = {Die Kombination von fortschrittlichen Materialien und kontrolliertem Oberfl{\"a}chendesign mit komplexen Proteinen aus der nat{\"u}rlichen Photosynthese ist derzeit eines der Hauptthemen bei der Entwicklung von Biohybridsystemen und Biophotovoltaik. In dieser Studie werden transparente makropor{\"o}se Indium-Zinn-Oxid-(μITO-) Elektroden mit dem trimeren Superkomplex Photosystem I (PSI) aus dem Cyanobakterium Thermosynechococcus elongatus sowie dem kleinen Redoxprotein Cytochrom c (Cyt c) kombiniert, um neuartige und effiziente biohybride Photokathoden herzustellen. Mit diesen bis zu 40 μm hohen 3D-Strukturen k{\"o}nnen beide Proteine in einer ann{\"a}hernden Monolage abgeschieden werden und die elektrische Kommunikation mit der Elektrode kann erzielt werden. Der generierte Photostrom folgt dabei linear der kontrollierbaren Schichtdicke der μITO-Elektrode, wobei Stromdichten von bis zu 150 μA cm -2 erhalten werden. Eine effiziente elektrische Kopplung der Proteine kann durch die hohe interne Quanteneffizienz von 30 \% gezeigt werden.}, language = {de} } @misc{KoberHanschkeMacholzetal.2018, author = {Kober, Liane and Hanschke, Christian and Macholz, Rainer and Frohme, Marcus}, title = {Die einfache Extraktion und Quantifizierung von sprengstofftypischen Verbindungen in Bodenproben {\"u}ber LC-MS/MS}, series = {Wissenschaftliche Beitr{\"a}ge 2018}, volume = {22}, journal = {Wissenschaftliche Beitr{\"a}ge 2018}, issn = {0949-8214}, doi = {10.15771/0949-8214_2018_1}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:526-opus4-10219}, pages = {7 -- 16}, year = {2018}, abstract = {Die Kontamination von Fl{\"a}chen mit R{\"u}stungsaltlasten durch sprengstofftypische Verbindungen (STV) wie 2,4,6-Trinitrotoluol (TNT) ist nach wie vor ein großes Risiko f{\"u}r Gesundheit und Umwelt. Die Hochdruckfl{\"u}ssigchromatographie (HPLC) ist in Kombination mit der Tandem-Massenspektrometrie (MS/MS) ein besonders sensitives und exaktes Verfahren zur Konzentrationsbestimmung derartiger Umweltkontaminanten. Jedoch ben{\"o}tigen die derzeit eingesetzten Methoden zur Extraktion und Quantifizierung viel Zeit und Bodenmaterial. Wir haben daher eine 20-min{\"u}tige LC-MS/MS-Methode entwickelt, die in einer Probe simultan acht STV qualitativ und quantitativ nachweisen kann. Im Vergleich zur EPA-Referenzmethode konnte bei der Extraktion die eingesetzte Bodenmenge von 10 g auf 2 g reduziert und die Extraktionsdauer von 18 h auf 30 min verk{\"u}rzt werden. Die abschließende Validierung zeigte eine gute Reproduzierbarkeit und mit etablierten Protokollen vergleichbare Detektions- (LOD) und Quantifizierungslimits (LOQ), so dass die optimierten Methoden f{\"u}r eine schnelle und zuverl{\"a}ssige Analyse von Realproben eingesetzt werden konnten.}, language = {de} }