TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Seifert, Felix
A1  - Thiemann, Alexander
A1  - Pospisil, Heike
A1  - Scholten, Stefan
T1  - Re-annotation of the maize oligonucleotide array
JF  - Maydica
N2  - The microarray technology has become an established approach for large-scale gene expression analysis with mature protocols for sample, microarray, and data processing. The maize oligonucleotide array (maizearray) is one of the few microarray platforms designed for genome-wide gene expression analysis in Zea mays L. Many datas-ets addressing various genetic, physiological and developmental topics generated with this platform are available. The original 57,452 microarray probes were compiled based on expressed sequence tags (ESTs). Meanwhile the maize genome sequence became available providing the possibility for an improved annotation of the microar-ray probe set. In this study we determined the genome positions of all maizearray probes to obtain current gene annotations and generated current Gene Ontology (GO) annotations. These new data allow tracing redundancy of the probe set and interfering cross-hybridizations, and doubled the number of genes with functional GO data. Our re-annotation will largely improve the functional analysis of available and future datasets generated on this microarray platform.
AB  - The microarray technology has become an established approach for large-scale gene expression analysis with mature protocols for sample, microarray, and data processing. The maize oligonucleotide array (maizearray) is one of the few microarray platforms designed for genome-wide gene expression analysis in Zea mays L. Many datas-ets addressing various genetic, physiological and developmental topics generated with this platform are available. The original 57,452 microarray probes were compiled based on expressed sequence tags (ESTs). Meanwhile the maize genome sequence became available providing the possibility for an improved annotation of the microar-ray probe set. In this study we determined the genome positions of all maizearray probes to obtain current gene annotations and generated current Gene Ontology (GO) annotations. These new data allow tracing redundancy of the probe set and interfering cross-hybridizations, and doubled the number of genes with functional GO data. Our re-annotation will largely improve the functional analysis of available and future datasets generated on this microarray platform.
KW  - maize oligonucleotide array
KW  - microarray
KW  - annotation
Y1  - 2012
SN  - 0025-6153
SS  - 0025-6153
U6  - https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-6178
UN  - https://nbn-resolving.org/urn:nbn:de:kobv:526-opus4-6178
UR  - https://journals-crea.4science.it/index.php/maydica/article/view/628
VL  - 57
IS  - 1
SP  - 49
EP  - 55
ER  -